Urinary 8-hydroxy-2 -deoxyguanosine(8-OHdG) is an excellent marker of oxidative DNA damage.In this study,employing guanosine as dummy template a novel molecularly imprinted(MIP) monolithic capillary column had been sy...Urinary 8-hydroxy-2 -deoxyguanosine(8-OHdG) is an excellent marker of oxidative DNA damage.In this study,employing guanosine as dummy template a novel molecularly imprinted(MIP) monolithic capillary column had been synthesized,and that was used as medium of in-tube solid phase microextraction(SPME).Coupled with capillary electrophoresis-electrochemical detection(CE-ECD),the system of extraction and detection of 8-OHdG in urinary sample had been developed.Because of its greater phase ratio combined with conv...展开更多
[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) com...[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS). The main compounds were alcohols( 54. 88%) and aldehydes( 19. 55%). [Results] The top five components with the highest relative content were phenylethyl alcohol( 12. 69%),geraniol( 9. 85%),citronellol( 8. 80%),nerol( 7. 84%) and 2-n-pentylfuran( 7. 45%). [Conclusions] Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) can provide basis for further development and utilization of R. davurica.展开更多
该研究优化了顶空固相微萃取-气相色谱双质谱(Headspace Solid Phase Microextraction with Gas Chromatography Coupled to Tandem Mass Spectrometry,HS-SPME-GC-MS/MS)联用技术检测新疆库车小白杏挥发性化合物组成的方法,并基于相对...该研究优化了顶空固相微萃取-气相色谱双质谱(Headspace Solid Phase Microextraction with Gas Chromatography Coupled to Tandem Mass Spectrometry,HS-SPME-GC-MS/MS)联用技术检测新疆库车小白杏挥发性化合物组成的方法,并基于相对香气活性值(Relative Odor Activity Value,ROAV)鉴定其关键性香气物质,对小白杏呈香属性指纹进行分析。结果表明:使用50/30μm聚二乙烯基苯/碳吸附剂/聚二甲基硅氧烷(Divinylbenzene/Carboxen/Polydimethylsiloxane,DVB/CAR/PDMS)的萃取纤维,萃取温度为65℃,萃取时间为50 min时,萃取效率最高。在该条件下,共鉴定出小白杏中含有的46种挥发性物质,其中醇类物质含量最高(34.57%),其次是酯类(33.77%)和醛类物质(24.77%)。ROAV分析表明,β-紫罗兰酮、γ-癸内酯、二氢-β-紫罗兰酮、芳樟醇、月桂烯以及α-紫罗兰酮为关键香气物质(ROAV>1),赋予了小白杏浓郁的果香、花香以及木香。呈香属性指纹分析表明,与吊干杏相比,小白杏的青草香、果香、椰子香、柑橘香以及花香更为强烈,只有木香较弱。该研究确定了新疆库车小白杏中含有的关键香气成分,可为探索具有小白杏风味的产品研发提供参考依据。展开更多
为研究不同加工工艺紫娟白茶的关键香气化合物,采用顶空固相微萃取-气相色谱-质谱联用技术(Headspace solid-phase micro extraction and gas chromatography mass spectrometry,HS-SPME-GC-MS),结合感官审评、PCA验证、OPLS-DA分析以...为研究不同加工工艺紫娟白茶的关键香气化合物,采用顶空固相微萃取-气相色谱-质谱联用技术(Headspace solid-phase micro extraction and gas chromatography mass spectrometry,HS-SPME-GC-MS),结合感官审评、PCA验证、OPLS-DA分析以及相对香气活度值(Relative odor activity value,rOAV),分析筛选紫娟白茶的关键香气化合物。结果表明:共检测出82种主要香气组分,以醇类、酯类、杂环及芳香族化合物为主,芳樟醇、水杨酸甲酯、苯甲醇、β-紫罗兰酮等相对含量较高,rOAV法分析结果显示:1-辛烯-3-醇、α-紫罗兰酮、苯乙醛、水杨酸甲酯等9种挥发性有机化合物对自然萎凋的紫娟白茶(TZW)花果香馥郁且带毫香、辛香、药香的香气形成具有较大贡献,1-辛烯-3-醇、α-紫罗兰酮、β-紫罗兰酮、苯甲醛等9种挥发性有机化合物对复式萎凋紫娟白茶(CZW)果香浓郁且带花香、辛香的香气形成具有较大贡献。OPLS-DA分析结合rOAV发现1-辛烯-3-醇和水杨酸甲酯是TZW与CZW香气差异的关键化合物。综上所述,自然萎凋方式加工的紫娟白茶花果香更加浓郁,可以有效提高紫娟白茶的香气品质。展开更多
基金the support of the National Natural Science Foundation of China(No.20575051).
文摘Urinary 8-hydroxy-2 -deoxyguanosine(8-OHdG) is an excellent marker of oxidative DNA damage.In this study,employing guanosine as dummy template a novel molecularly imprinted(MIP) monolithic capillary column had been synthesized,and that was used as medium of in-tube solid phase microextraction(SPME).Coupled with capillary electrophoresis-electrochemical detection(CE-ECD),the system of extraction and detection of 8-OHdG in urinary sample had been developed.Because of its greater phase ratio combined with conv...
基金Supported by Key Science and Technology Project of Gansu Province(1302NKDA028)Science and Technology Planning Project of Lanzhou(2010-1-239+2 种基金 2016-3-4)Talent Project of Lanzhou Science and Technology Bureau(2015-RC-87)Project of Science and Technology Cooperation between Gansu Academy of Agricultural Sciences and Local Areas(2017GAAS63)
文摘[Objectives] To determine the aromatic components of Rosa davurica Pall. [Methods] 42 kinds of aromatic components were identified from the flowers of R. davurica by headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS). The main compounds were alcohols( 54. 88%) and aldehydes( 19. 55%). [Results] The top five components with the highest relative content were phenylethyl alcohol( 12. 69%),geraniol( 9. 85%),citronellol( 8. 80%),nerol( 7. 84%) and 2-n-pentylfuran( 7. 45%). [Conclusions] Headspace solid phase microextraction( HS-SPME) combined with gas chromatography-mass spectrometry( GC-MS) can provide basis for further development and utilization of R. davurica.
文摘该研究优化了顶空固相微萃取-气相色谱双质谱(Headspace Solid Phase Microextraction with Gas Chromatography Coupled to Tandem Mass Spectrometry,HS-SPME-GC-MS/MS)联用技术检测新疆库车小白杏挥发性化合物组成的方法,并基于相对香气活性值(Relative Odor Activity Value,ROAV)鉴定其关键性香气物质,对小白杏呈香属性指纹进行分析。结果表明:使用50/30μm聚二乙烯基苯/碳吸附剂/聚二甲基硅氧烷(Divinylbenzene/Carboxen/Polydimethylsiloxane,DVB/CAR/PDMS)的萃取纤维,萃取温度为65℃,萃取时间为50 min时,萃取效率最高。在该条件下,共鉴定出小白杏中含有的46种挥发性物质,其中醇类物质含量最高(34.57%),其次是酯类(33.77%)和醛类物质(24.77%)。ROAV分析表明,β-紫罗兰酮、γ-癸内酯、二氢-β-紫罗兰酮、芳樟醇、月桂烯以及α-紫罗兰酮为关键香气物质(ROAV>1),赋予了小白杏浓郁的果香、花香以及木香。呈香属性指纹分析表明,与吊干杏相比,小白杏的青草香、果香、椰子香、柑橘香以及花香更为强烈,只有木香较弱。该研究确定了新疆库车小白杏中含有的关键香气成分,可为探索具有小白杏风味的产品研发提供参考依据。
文摘为研究不同加工工艺紫娟白茶的关键香气化合物,采用顶空固相微萃取-气相色谱-质谱联用技术(Headspace solid-phase micro extraction and gas chromatography mass spectrometry,HS-SPME-GC-MS),结合感官审评、PCA验证、OPLS-DA分析以及相对香气活度值(Relative odor activity value,rOAV),分析筛选紫娟白茶的关键香气化合物。结果表明:共检测出82种主要香气组分,以醇类、酯类、杂环及芳香族化合物为主,芳樟醇、水杨酸甲酯、苯甲醇、β-紫罗兰酮等相对含量较高,rOAV法分析结果显示:1-辛烯-3-醇、α-紫罗兰酮、苯乙醛、水杨酸甲酯等9种挥发性有机化合物对自然萎凋的紫娟白茶(TZW)花果香馥郁且带毫香、辛香、药香的香气形成具有较大贡献,1-辛烯-3-醇、α-紫罗兰酮、β-紫罗兰酮、苯甲醛等9种挥发性有机化合物对复式萎凋紫娟白茶(CZW)果香浓郁且带花香、辛香的香气形成具有较大贡献。OPLS-DA分析结合rOAV发现1-辛烯-3-醇和水杨酸甲酯是TZW与CZW香气差异的关键化合物。综上所述,自然萎凋方式加工的紫娟白茶花果香更加浓郁,可以有效提高紫娟白茶的香气品质。
