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题名水母雪莲细胞悬浮培养合成黄酮及抗氧化活性(英文)
被引量:12
- 1
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作者
刘永刚
高敏
崔建云
刘春朝
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机构
中国科学院过程工程研究所生化工程国家重点实验室
中国农业大学食品科学与营养工程学院
中国科学院研究生院
中国农业大学食品科学与营养工程学院
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出处
《西北植物学报》
CAS
CSCD
北大核心
2005年第7期1421-1427,共7页
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文摘
对水母雪莲细胞悬浮培养过程中细胞生长、黄酮积累和底物消耗的动力学过程进行了研究。经15d液体培养可获得最大生物量干重和黄酮产量分别为17.2g·L-1和607.8mg·L-1,通过调控基本培养基种类和有机添加物可提高雪莲细胞的生长和黄酮积累。获得的水母雪莲细胞培养物具有明显的抗氧化活性,其抗氧化活性与雪莲细胞中的黄酮含量相关。
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关键词
水母雪莲
黄酮
悬浮细胞
抗氧化活性
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Keywords
Saussurea medusa
flavonoid
suspended cells
antioxidant Saussurea medusa Maxim.is a valuable and traditional Chinese herb of the Compositae family.The whole plant can be used in treating rheumatoid arthritis,regulating menstrual function,and inhibiting ageing and the growth of cancer cells.Phytochemical analysis of S.medusa shows that it is a rich source of total flavonoids including jaceosidin,hispidulin,quercelin and various novel compounds that are difficult to obtain from other plants.These flavonoids are the main medicinally active constituents of S.medusa which in particular display effective anti-tumor and anti-inflammation properties and delay senility^([1]).However,clinical demand for this plant and its total flavonoids products are hardly met due to a very limited supply.The wild S.medusa only grows at rocky beaches of 3 900^(5 100 m) above sea level with a special climate and cultivation of this species in a normal climate has been unsuccessful so far.Owing to over-exploitation of the wild plants for commercial purposes and the difficulty of cultivation, S.medusa is now almost extinct in China.It was listed as the second grade national protected wild plant in China.To solve these problems,large-scale cultivation of plant cells would be a promising alternative to produce useful phytochemicals of S.medusa ,such as flavonoids^([2^4]). The objective of the current study was to obtain a systemic understanding of cell growth,flavonoid accumulation and nutriment exhaustion in the established S.medusa cell suspension cultures,and to set a foundation for efficient large-scale cultivation for biosynthesis of flavonoids.Furthermore,the antioxidant activity of S.medusa cell suspension cultures using the DPPH scavenging assay system was firstly evaluated for further medicinal applications.
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分类号
Q946.8
[生物学—植物学]
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题名郑州市常色叶植物应用情况调查及综合评价
- 2
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作者
孙飞
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机构
郑州市城市园林科学研究所
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出处
《绿色科技》
2022年第11期110-113,125,共5页
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文摘
对郑州市城市绿化中应用的常色叶植物进行了调查,应用层次分析法从观赏表现性、抗逆性、生长性状3个方面11个相关指标进行了综合评价并划分为4个等级。结果表明:常色叶植物共计41种,其中乔木占比14.63%,灌木占比39.02%,草本占比36.59%,藤本占比9.76%;综合评价结果为:Ⅰ级包括16个品种,Ⅱ级包括14个品种,Ⅲ级包括8个品种,Ⅳ级包括3个品种。
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关键词
城市绿化
常色叶植物
应用调查
层次分析法
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Keywords
urban greening
evergreen plants
including 3 species of grade iv
application survey
Analytic Hierarchy Process
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分类号
S685.12
[农业科学—观赏园艺]
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