共表达分子伴侣蛋白提高III型普鲁兰水解酶在短小芽孢杆菌中的分泌表达及发酵优化

本研究旨在通过共表达分子伴侣蛋白以及优化发酵条件来提高III型普鲁兰水解酶(TK-PUL)在短小芽孢杆菌中的分泌表达水平。通过构建共表达TK-PUL和分子伴侣蛋白的多种重组短小芽孢杆菌,并以胞外酶活为指标对其进行筛选,确定最有利于TK-PUL分泌表达的分子伴侣蛋白及对应的重组短小芽孢杆菌。在此基础上采用单因素实验和响应面法优化重组短小芽孢杆菌的发酵条件。结果表明,共表达分子伴侣蛋白PrsA^(Ba)的重组短小芽孢杆菌的胞外酶活达到98.79 U/mL,提高了0.31倍。该重组短小芽孢杆菌的最佳培养基包括19.65 g/L葡萄糖、21.46 g/L酵母提取物、12.01 g/L MgCl_(2)·6H_(2)O、9.02 g/L脯氨酸、0.01 g/L FeSO4·7H_(2)O、0.01 g/L MnSO4·4H_(2)O、0.001 g/L ZnSO4·7H_(2)O。在发酵温度为35℃、起始发酵pH为7.0的条件下,该重组短小芽孢杆菌于最佳培养基中培养66 h时,其胞外酶活达到192.68 U/mL,提高了1.56倍。本研究通过共表达分子伴侣蛋白和发酵优化实现了TK-PUL在短小芽孢杆菌的高效分泌表达,为TK-PUL的应用提供了基础。

混合牙列期骨性III类错合与骨性I类错合基骨及牙弓对比研究

目的:通过测量混合牙列期骨性III类错合患者与骨性I类错合患者的锥形束计算机断层扫描(Cone beam computed tomography, CBCT),对比分析上下颌牙弓、基骨弓长度与宽度差异及协调性,以期为替牙期骨性III类错合畸形治疗提供参考依据。方法:选择替牙列期骨性III类错合与骨性I类错合各30例,通过Dolphin软件处理收集到的DICOM (Digital Imaging and Communications in Medicine)医学图像数据,行三维重建并测量上下颌牙弓、基骨弓的长度与宽度,计算同一牙段牙弓宽度与基骨弓宽度之间的差值,比较骨性III类与骨性I类错牙合畸形牙弓与基骨弓宽度差值的差异。结果:1) 骨性III类错合组上颌牙弓及基骨弓长度均小于骨性I类错合组,下颌基骨弓长度大于骨性I类错合组,且差异有统计学意义(P 0.05)。2) 骨性III类错合组下颌前段及中段基骨弓宽度大于骨性I类错合组,差异有统计学意义(P 0.05)。3) 骨性III类错合组上颌牙弓与基骨弓宽度的差值均大于骨性I类错合组,下颌牙弓与基骨弓宽度的差值均小于骨性I类错合组,其中上颌后段牙弓宽度与基骨弓宽度差值差异有统计学意义(P < 0.05)。结论:混合牙列期骨性III类错合存在牙弓、基骨弓不调,上颌可扩大基骨弓,下颌可扩大牙弓,以期改善牙弓与基骨弓的协调性。

III型胶原蛋白在乳腺癌中的研究进展

乳腺癌(Breast cancer, BC)于2020年在全球新增癌症病例成为了最常见癌症,且发病率逐年升高。胶原蛋白是构成细胞外基质(Extracellular Matrix, ECM)的主要成分,近年来的研究发现III型胶原蛋白(Collagen Type III, COLIII)在乳腺癌的发生、发展过程中发挥重要作用,并对肿瘤微环境(Tumor micro-environment, TME)的维持有重要意义。其中,癌症相关成纤维细胞(Cancer-associated fibroblasts, CAFs)在建立和重塑ECM结构中扮演关键角色,调控肿瘤细胞通过TME入侵的进程。但由于该体系庞大,通过分泌细胞因子和趋化因子与癌细胞相互作用的机制复杂,各成分及其亚型在癌症进展中的角色尚存争议。本文基于TME在乳腺恶性肿瘤中的研究现状以及作为ECM中重要成分的COLIII的性质结构、形态分布,分析COLIII通过改变ECM的机械力与刚度影响乳腺癌行为的机制,同时充分讨论特殊结构、来源的COLIII在微环境中的不同功能及作用方式,评估其应用于癌症治疗的可能性。

清解扶正颗粒方药治疗湿热下注型III型前列腺炎的临床疗效观察和机制探讨

本研究旨在观察清解扶正颗粒方药治疗湿热下注型III型前列腺炎的临床疗效,并探讨其作用机制。方法 选取晋江市中医院泌尿男科门诊的80例湿热下注型III型前列腺炎患者作为研究对象,分为治疗组、对照组,每组40例。治疗组接受清解扶正颗粒方药治疗,对照组接受常规治疗。观察周期为4周,观察指标包括症状评估、前列腺液分析和血清炎症因子分析。结果 治疗组的症状积分指数显著降低,卵磷脂小体数量增加,前列腺液PH值升高,白细胞数目减少,血清炎症因子水平降低,氧化应激产物一氧化氮减少,超氧化物歧化酶等抗氧化物质活性提高,嗜铬粒蛋白A水平降低,肝肾功能无明显差异。治疗组的症状改善程度显著优于对照组和联合组,差异有统计学意义。结论 清解扶正颗粒方药治疗湿热下注型III型前列腺炎具有良好的临床疗效。其作用机制可能包括抗菌、消肿止痛和免疫调节等多种作用。清解扶正颗粒方药具有较低的毒副作用和良好的安全性。进一步的研究和临床实践是必要的,以验证其长期疗效和复发率,并进一步揭示其作用机制。

Phase composition,conductivity,and sensor properties of cerium-doped indium oxide

The hydrothermal synthesis of In_(2)O_(3)and CeO_(2)–In_(2)O_(3)is investigated as well as the properties of sensor layers based on these compounds.During the synthesis of In_(2)O_(3),intermediate products In(OH)_(3)and InOOH are formed,which are the precursors of stable cubic(c-In_(2)O_(3))and metastable rhombohedral(rh-In_(2)O_(3))phases,respectively.A transition from c-In_(2)O_(3)to rh-In_(2)O_(3)is observed with the addition of CeO_(2).The introduction of cerium into rh-In_(2)O_(3)results in a decrease in the sensor response to hydrogen,while it increases in composites based on c-In_(2)O_(3).The data on the sensor activity of the composites correlate with XPS results in which CeO_(2)causes a decrease in the concentrations of chemisorbed oxygen and oxygen vacancies in rh-In_(2)O_(3).The reverse situation is observed in composites based on c-In_(2)O_(3).Compared to In_(2)O_(3)and CeO_(2)–In_(2)O_(3)obtained by other methods,the synthesized composites demonstrate maximum response to H_(2)at low temperatures by 70–100℃,and have short response time(0.2–0.5 s),short recovery time(6–7 s),and long-term stability.A model is proposed for the dependence of sensitivity on the direction of electron transfer between In_(2)O_(3)and CeO_(2).

Soil Profile Concentration Distributions of Aluminum, Gallium, Indium and Thallium across Southeastern Missouri

The soil chemistry of gallium, indium, and thallium is not well defined, particularly with emerging evidence that these elements have toxic properties and may influence food safety. The purpose of this investigation was to estimate the soil concentrations of gallium, indium, and thallium and determine if these elements have a soil chemistry like aluminum and therefore demonstrate significant concentration correlations with aluminum. Twenty-seven soil series were selected, and the elemental concentrations were determined using aqua regia digestion with analytical determination performed using inductively coupled plasma emission-mass spectroscopy. The concentrations of gallium, indium, and thallium generally compared with the known literature. Aluminum-gallium and aluminum-thallium exhibited significant concentration correlations across the soil horizons of the sampled soils. Aluminum, gallium, and thallium did demonstrate concentration increases in soil horizons having illuviation of phyllosilicates, implying these phyllosilicates have adsorption and isomorphic substitution behaviors involving these elements.

Target-induced Trivalent G-quadruplex/hemin DNAzyme for Colorimetric Detection of Hg^(2+) Based on an Exonuclease III Assisted Catalytic Hairpin Assembly

Mercury ion(Hg^(2+)),a highly noxious of heavy metalion,has detrimental effects on the ecological environment and human health.Herein,we have developed an exonuclease III(Exo III)assisted catalytic hairpin assembly formation of a trivalent G-quadruplex/hemin DNAzyme for colorimetric detection of Hg^(2+).A hairpin DNA(Hr)was designed with thymine-Hg^(2+)-thymine pairs that catalyzed by Exo III is prompted to happen upon binding Hg^(2+).A released DNA fragment triggers the catalytic assembly of other three hairpins(H1,H2,and H3)to form many trivalent G-quadruplex/hemin DNA enzymes for signal output.The developed sensor shows a dynamic range from 2 pM to 2μM,with an impressively low detection limit of 0.32 pM for Hg^(2+)detection.Such a sensor also has good selectivity toward Hg^(2+)detection in the presence of other common metal ions.This strategy shows the great potential for visual detection with portable type.

中青年视网膜静脉阻塞与同型半胱氨酸、抗凝血酶III相关研究

目的:研究分析中青年视网膜静脉阻塞(Retinal Vein Occlusion, RVO)患者同型半胱氨酸(Homocysteine, Hcy)及抗凝血酶III (Antithrombin III, AT-III)水平,两者是否作为致病危险因素参与RVO发病。分析视网膜中央静脉阻塞(CRVO)和视网膜分支静脉阻塞(BRVO)危险因素的差异,为治疗和预防该病提供客观依据。方法:1) 所有研究对象选自2021年10月至2023年12月期间就诊于内蒙古自治区人民医院眼科的年龄 P > 0.05)。三组患者之间在Hcy、AT-III、胆固醇(CHOL)、叶酸、维生素B12、D-二聚体(D-D)、纤维蛋白降解产物(FDP)水平差异均有统计学意义(P P P < 0.001)。结论:1) Hcy水平升高和AT-III活性缺乏为中青年CRVO和BRVO的危险因素。2) 相对于BRVO患者,CRVO组中Hcy水平升高和AT-III缺乏的值更为显著。

影响II-III期结直肠癌患者生存的因素分析

分析影响II-III期结直肠癌患者生存的因素。方法 回顾性分析柳州市鹿寨县人民医院进行治疗的II-III期结直肠癌患者(110例),2017年5月至2021年8月为病例选取时间,并对所有纳入人员进行为期2年的随访,以分析影响其生存时间的因素。结果 性别、年龄、文化程度、烟酒史、家族史、肿瘤位置均P>0.05,非影响此类患者生存的高危指标;肿瘤分化程度、TNM分期、脉管侵犯、神经侵犯及手术方式均P<0.05,为影响此类患者生存的高危指标;COX逐步回归法结果显示,低肿瘤分化程度、TNM Ⅲ期、姑息性切除术、有脉管侵犯及神经侵犯均可作为影响II-III期结直肠癌患者生存的独立危险因素,P<0.05。结论 在II-III期结直肠癌患者中,低肿瘤分化程度、TNM Ⅲ期、姑息性切除术、有脉管侵犯及神经侵犯均可作为此疾病患者生存时间的影响因素,并可作为临床评估此疾病的相关指标。

改良前正中入路微型钢板内固定治疗II、III型尺骨冠状突骨折

对II、III型尺骨冠状突骨折通过改良前正中入路复位冠状突骨折块,微型钢板内固定的临床效果进行探讨。方法 分析我院骨伤科2018年8月至2020年8月的12例接受改良前正中入路,微型钢板内固定II、III型尺骨冠状突骨折患者的术中、术后等情况,并通过梅奥肘关节功能评分进行评定,以探讨该入路方式的临床效果。结果 手术后的随访时间平均超过6个月,达到平均8.2个月。所有患者的手术切口均成功愈合,骨折愈合时间在7-11周之间,平均为8.1周。肘部的活动度表现良好,平均屈曲活动度达到135.4°(110°~144°),伸直活动度平均为6°(-4°~13°),前臂的旋前和旋后活动度分别平均为76.2°(65°~86°)和80.3°(72°~84°)。根据梅奥肘关节功能评分,平均分为87.2分(74~100),其中有7例评为优,4例为良,1例为可,优良率达到91.7%。改良前正中入路治疗尺骨冠状突骨折表现出创伤小、暴露充分、安全性高、操作简单、关节功能恢复良好等优势,值得在临床中推广和应用。

Oxone-Mediated Oxidative Esterification of Heterocyclic Aldehydes Using Indium(III) Triflate

Recent investigations have shown the oxone-mediated oxidative methyl esterification of benzaldehyde derivatives using methanol. The reactions were accelerated in the presence of indium(III) triflate, a trivalent indium reagent, in many cases. Based on this method of methyl esterification of benzaldehyde derivatives, we further explored an application to heterocyclic aldehydes. The reactions were examined using methanol as well as other alcohols in order to establish a suitable range.

锑氧化菌Pseudomonas sp.AO-1的分离鉴定及其对Sb(III)的氧化性能

采用抗性筛选法从锡矿山筛选出一株锑氧化菌,并利用分子生物学技术对其进行鉴定;考察了其氧化Sb()Ⅲ的性能和氧化次生矿物的特征.结果表明:锑氧化菌属于假单胞菌属(Pseudomonas),将其命名为Pseudomonas sp.AO-1(简称:AO-1);影响AO-1氧化Sb(Ⅲ)的因素主要有溶液pH值、溶解氧和铁锰氧化物(单质铁、FeCl_(3)和MnO_(2))等;AO-1在好氧和缺氧条件下均能氧化Sb(Ⅲ),好氧氧化Sb(Ⅲ)的米门常数Km和最大氧化速率Vmax值分别为393.05µmol/L和0.271µmol/(L·min),体现了较强的锑氧化性;AO-1和铁锰氧化物的耦合作用能促进Sb(Ⅲ)的氧化,且铁锰氧化物促进AO-1氧化Sb(Ⅲ)的速率依次为:FeCl_(3)>MnO_(2)>单质铁;AO-1和铁锰氧化物耦合氧化Sb(Ⅲ)生成含Sb(Ⅴ)的次生矿物,次生矿物会加速Sb(Ⅲ)的氧化以及影响锑在环境中的迁移转化.菌株AO-1的锑氧化性能良好,对于锑的生物化学转化和土壤微生物修复的应用有重要意义.

Highly-Efficient Conversion of Primary Amides to Nitriles Using Indium(III) Triflate as the Catalyst

Indium(III) triflate, a trivalent indium reagent, was shown to be a highly-efficient catalyst for the conversion of primary amides to the corresponding nitriles. The successful reactions required 5 mol% of indium(III) triflate, and toluene was proved to be the most suitable solvent. Various amides were subjected to this method, and each produced the corresponding nitriles in excellent yields.

Oxone-Mediated Preparation of Ester Derivatives Using Indium(III) Triflate and Various Alcohols

Esters are known as one of the most fundamental chemical moieties and also essentially useful components, especially for medicinal agents. Herein, using benzaldehyde and its derivatives as starting materials, Oxone-mediated preparation of ester derivatives in the presence of a catalytic amount of indium(III) triflate is described. Alcohols with various chain lengths, which functioned as solvents and substrates, were examined. Overall, the oxidative esterification starting with benzaldehyde derivatives possessing electron withdrawing groups proceeded smoothly and gave sufficient yields, in comparison to the reactions with the derivatives having electron donating groups.

Synthesis and Structure of a Hydrogen-bonding Assembled Three-dimensional Supramolecular Indium(III) Compound Involving One-dimensional Helices

The title compound [In（H2ip）（pdc）（H2O）] （H3ip = 5-hydroxyisophthalic acid, H2Pdc = pyridine-2,6-dicarboxylic acid） has been synthesized and characterized by single-crystal X-ray diffraction analysis. It crystallizes in monoclinic, space group P21/c with a = 13.830（8）, b = 6.488（4）, c = 17.632（10）A^°, β = 92.510（10）°, C15H10InNO10, Mr= 479.06, V = 1580.6（15）A^°3, Z = 4, De= 2.013 g/cm^3, F（000） = 944,μ = 1.557 mm^-1, the final R = 0.0413 and wR = 0.0793 for 2950 observed reflections with I 〉 2σ（I）. The In（Ⅲ） ion is seven-coordinated in a slightly distorted penta-bipyramidal geometry. The mixed ligands connect the In（Ⅲ） ions into 21 helical chains along the [010] direction, and the hydrogen bonds assemble the chains into a three-dimensional supramolecular network.

重组III型人源化胶原蛋白修护贴敷料联合光子嫩肤对不适症状以及肤质改善的研究

评价重组III型人源化胶原蛋白修护贴敷料在光子嫩肤术后缓解不适症状以及对肤质改善的作用。方法 选择2022年7月—2023年2月于本医院门诊接受光子嫩肤治疗的120例患者,根据1:1简单随机分配原则把患者分为联合组与对照组各60例采用光子嫩肤治疗。联合组给予重组III型人源化胶原蛋白修护贴敷料联合光子嫩肤治疗,对照组用保湿贴敷料代替重组III型人源化胶原蛋白修护贴敷料,同样联合光子嫩肤治疗,两组都治疗观察4周。结果 1、联合组和对照组在术后第2、6、10天的不适症状评分均呈逐渐下降趋势,其中联合组改善程度更为明显。对光子术后即刻与术后第8天评分差值对比,联合组相较对照组具有统计学差异。2、首次进行光子术前,第二次光子术前,第三次光子术前,第12周结束对肤质情况信息录取。联合组和对照组,肤质均有所改善。第12周较光子术前改善对比,联合组改善更为明显,且P<0.05,差异有统计学意义。结论 1、重组III型人源化胶原蛋白修护贴敷料可明显缓解光子术后面部皮肤产生的不适症状,有良好的辅助治疗作用。2、重组III型人源化胶原蛋白修护贴敷料联合光子嫩肤能更好的改善肤质,起到肤质改善的作用。

Dehydrosulfurization of Aromatic Thioamides to Nitriles Using Indium(III) Triflate

The efficient dehydrosulfurization of thioamides to nitriles was carried out using indium(III) triflate as a catalyst. Based on the results of the initial study, the optimal reaction conditions required 5 mol% of indium(III) triflate with toluene as the practical solvent. Various thioamides were successfully converted to nitriles in high yields.

DFR、抗凝血酶III概况及其在急性肺栓塞中的应用

急性肺栓塞是多种因素共同作用下发生的、可严重危及生命的一种疾病。因其临床表现、体征缺乏特异性,单一检测往往不足以满足诊断需要,误诊率及漏诊率大,病死率高。故在疾病初期采取何种有效的方式进行诊断已成为临床研究热题。D-二聚体为反映纤溶亢进的指标,纤维蛋白原为凝血的指标,DFR作为D-D与Fbg的比值,在一定程度上可以反映纤溶与凝血之间的平衡。抗凝血酶III具有显著的抗凝血作用,其水平下降影响机体抗凝作用,促进血栓形成。

Gartland III型伸直型儿童肱骨髁上骨折的治疗进展

Gartland III型伸直型儿童肱骨髁上骨折是临床上一种较为严重肘部骨折,常合并神经血管损伤,如果治疗不当,会导致严重的并发症,对患儿的身心健康造成不利的影响。由于其骨折端之间骨皮质完全断裂,有明显的侧方和(或)旋转移位,相对不稳定,所以闭合复位相对难度较大。本文将通过查阅近年来关于Gartland III型伸直型儿童肱骨髁上骨折治疗研究进展的相关文献作一综述,旨在加深对该型骨折临床特征的认识并系统阐述其诊治方式的发展现状,为临床决策提供科学依据。

Study on the occurrence state of indium in sphalerite of Dulong Sn–Zn–In polymetallic deposit,Southwest China

The Dulong deposit,located in the Laojunshan area of southeastern Yunnan,China,is an important polymetallic deposit due to its high reserves of tin,zinc,and indium.The occurrence state of indium is critical for understanding its supernormal enrichment mechanism.Previous studies investigated the occurrence state of indium(including the valence state)based on the indium content in sphalerite and the correlation between metal concentrations.However,more evidence is needed to better constrain indium occurrence at the micro-,nano-,or even atomic scale.In this study,EPMA-FIB-SEM-TEM and XPS techniques were employed to investigate the indium distribution characteristics and occurrence state in sphalerite from the Dulong Sn–Zn–In polymetallic deposit.The maximum concentration of indium in the indium-rich sphalerite samples is 0.37%,and the results of the EPMA analysis showed a relatively homogeneous distribution of indium in sphalerite.The FIB-SEM-TEM results demonstrated that the lattice stripes of sphalerite were periodically and continuously distributed at the nanoscale,confirming that sphalerite in the deposit was an excellent single crystal structure,and the peak heights of the various characteristic peaks of indium in the EDX spectra were relatively close to each other,with no distinct peaks of high indium content.In addition,the XPS results indicate that the element valence state of indium in sphalerite is In^(3+),and it combines with S^(2-)to form a bond.These results indicate that indium in sphalerite of the Dulong deposit is uniformly distributed at both the micro-and nanoscale,and there is no indium-independent mineral.In^(3+)enters the crystal lattice of sphalerite by replacing Zn2+in the form of isomorphic substitution.