Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution a...Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca^2+-ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca^2+- ATPase activity in tender leaves were higher compared with those in the control (CK). Calcium nitrate and calcium chloride (ALe-Ca) and calcium phosphate and calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. The percentage of active calcium, calcium pectate, and water-soluble calcium increased, whereas the percentage of calcium phosphate and calcium carbonate decreased. When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The results indicated that IBA increased the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of stored Ca in plants. IBA promoted Ca^2+-ATPase activity and Ca^2+ uptake in tender shoots of M. hupehensis. It can improve the total Ca contents and the relative percentage of Ca.展开更多
In order to evaluate the effects of exogenous indole butyric acid (IBA) and callus formation on the antioxidant activity, total phenolics, and anthocyanin constituents of Morus nigra L. and M. alba L. cuttings, we i...In order to evaluate the effects of exogenous indole butyric acid (IBA) and callus formation on the antioxidant activity, total phenolics, and anthocyanin constituents of Morus nigra L. and M. alba L. cuttings, we investigated the variations before and after the treatment. The results indicate that anti-oxidant ability, total phenolic, and anthocyanin constituents of the callus stems of both Morus species were higher than those of non-callus forming species. There were also increases observed in anti-oxidant ability, total phenolic, and anthocyanin constituents of calli treated with IBA (1 000-3 000 mg/L).展开更多
基金The study was supported financially by the National Natural Science Foundation of China(30170655&30270923).
文摘Calcium (Ca) plays an important role in the metabolism of higher plants. Recently, research on Ca^2+ in plants has been focused especially at the cellular and molecular levels. Uptake, transport, and distribution are also very important for Ca to accomplish its function at the whole-plant level. In this experiment, one-year-old apple seedlings (M. hupehensis Rehd.) were investigated to determine the distribution of stored Ca, the different forms of Ca, and Ca^2+-ATPase activity after treatment with indole butyric acid (IBA). The results showed that the total Ca measured in mature leaves and Ca^2+- ATPase activity in tender leaves were higher compared with those in the control (CK). Calcium nitrate and calcium chloride (ALe-Ca) and calcium phosphate and calcium carbonate (HAC-Ca) decreased in both mature leaves and shoots, whereas water-soluble calcium (H2O-Ca), calcium pectate (NaCl-Ca), and calcium oxalate (HCl-Ca) increased. The percentage of active calcium, calcium pectate, and water-soluble calcium increased, whereas the percentage of calcium phosphate and calcium carbonate decreased. When treated with IBA, calcium fractions and percentage of the different forms of Ca was enhanced in 40 part per million (ppm) IBA compared with 20 ppm IBA and water. The results indicated that IBA increased the percentage of both active calcium (NaCl-Ca and H2O-Ca) in tender shoots and boosted the transportation of stored Ca in plants. IBA promoted Ca^2+-ATPase activity and Ca^2+ uptake in tender shoots of M. hupehensis. It can improve the total Ca contents and the relative percentage of Ca.
基金Publication of this paper is supported by the National Natural Science Foundation of China (30424813) and Science Publication Foundation of the Chinese Academy of Sciences.
文摘In order to evaluate the effects of exogenous indole butyric acid (IBA) and callus formation on the antioxidant activity, total phenolics, and anthocyanin constituents of Morus nigra L. and M. alba L. cuttings, we investigated the variations before and after the treatment. The results indicate that anti-oxidant ability, total phenolic, and anthocyanin constituents of the callus stems of both Morus species were higher than those of non-callus forming species. There were also increases observed in anti-oxidant ability, total phenolic, and anthocyanin constituents of calli treated with IBA (1 000-3 000 mg/L).