目的:通过检测桃红四物汤对创伤性股骨头坏死(trauma-induced osteonecrosis of the femoral head,TIONFH)模型大鼠骨组织BMP-4和Osterix表达的影响,探讨桃红四物汤延缓TIONFH病程的作用机制。方法:将35只SD大鼠适应性喂养1周后随机分...目的:通过检测桃红四物汤对创伤性股骨头坏死(trauma-induced osteonecrosis of the femoral head,TIONFH)模型大鼠骨组织BMP-4和Osterix表达的影响,探讨桃红四物汤延缓TIONFH病程的作用机制。方法:将35只SD大鼠适应性喂养1周后随机分为空白组(7只)和造模组(28只)。造模组按照经典股骨头血供剥离法进行造模,造模4周后随机抽取空白组1只、造模组4只进行对比,确认造模成功后,将造模组随机分为桃红四物汤高、中、低剂量组及模型组,共4组,每组6只。依据人与动物体表面积换算公式,桃红四物汤组分别灌服相应剂量的汤剂,另外两组灌服等量0.9%氯化钠注射液,每日2次,持续6周。干预6周后取材,观察并记录股骨头大体形态,进行骨组织HE染色,采用Real-time-PCR法和Western Blot法分别检测股骨头组织中BMP-4和Osterix的基因和蛋白表达情况。结果:经桃红四物汤干预后,桃红四物汤高、中剂量组股骨头病理情况优于模型组,次于空白组。Western Blot结果显示,与空白组比较,模型组大鼠股骨头组织BMP-4、Osterix蛋白表达水平明显降低(P<0.01);与模型组比较,桃红四物汤各剂量组BMP-4蛋白表达水平明显升高(P<0.05,P<0.01),桃红四物汤高、中剂量组Osterix蛋白表达水平明显升高(P<0.01),桃红四物汤低剂量组Osterix蛋白表达水平与模型组比较,差异无统计学意义(P>0.05)。Real-time-PCR结果显示,与空白组比较,模型组大鼠股骨头组织BMP-4、Osterix mRNA表达量明显降低(P<0.01);与模型组比较,桃红四物汤高、中剂量组BMP-4、Osterix mRNA表达量明显升高(P<0.01);桃红四物汤低剂量组BMP-4、Osterix mRNA表达与模型组比较,差异无统计学意义(P>0.05)。结论:桃红四物汤能改善股骨头病理形态,其机制可能与桃红四物汤提高成骨相关因子BMP-4、Osterix的表达从而促进骨修复有关。展开更多
Human Wharton's jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, ...Human Wharton's jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, a Wnt/13 catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that after induction, the spindleshaped or fibroblastlike Wharton's jelly mesenchymal stem cells changed into bulbous cells with numerous processes. Immunofluorescent cytochemical stain ing and reversetranscription PCR showed positive expression of retinal progenitor cell markers, Pax6 and Rx, as well as weakly downregulated nestin expression. These results demonstrate that Wharton's jelly mesenchymal stem cells are capable of differentiating into retinal progenitor cells in vitro.展开更多
基金supported by 2010 Com-advanced School Young Diaph Support Project of Heilongjiang Province,China, No. 1155G60
文摘Human Wharton's jelly mesenchymal stem cells were isolated from fetal umbilical cord. Cells were cultured in serumfree neural stem cellconditioned medium or neural stem cellconditioned medium supplemented with Dkk1, a Wnt/13 catenin pathway antagonist, and LeftyA, a Nodal signaling pathway antagonist to induce differentiation into retinal progenitor cells. Inverted microscopy showed that after induction, the spindleshaped or fibroblastlike Wharton's jelly mesenchymal stem cells changed into bulbous cells with numerous processes. Immunofluorescent cytochemical stain ing and reversetranscription PCR showed positive expression of retinal progenitor cell markers, Pax6 and Rx, as well as weakly downregulated nestin expression. These results demonstrate that Wharton's jelly mesenchymal stem cells are capable of differentiating into retinal progenitor cells in vitro.
基金Supported by the National Natural Science Foundation of China(31071413)the Key Scientific and Technological Project of Henan Province(122102110189)+2 种基金the Natural Science Foundation of Henan Province(2011B210002)the Project for the Youth of Henan Institute of Education(20090103)the Key Research Project of Institution of Higher Education(15A210020)~~