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Molecular cloning and functional analyses of low-temperature induced genes from Ammopiptanthus mongolicus
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作者 CAO Peng-xiu1,SONG Jian1,ZHOU Chun-jiang1,WENG Man-li1,JIN De-min1,ZHAO Feng1,LIU Jing2,FENG Dian-qi2,WANG Bin1(1.The State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,CAS,Beijing,100101,China 2.Taishan Branch of Shandong Academy of Forestry Sciences,Taian 271000,China) 《湖南农业大学学报(自然科学版)》 CAS CSCD 北大核心 2007年第S1期240-,共1页
Studies on the cold-responsive genes and cold signaling of woody species drop far behind in comparison to herbaceous plants.Due to similar lignified structure,perennial characteristic,and enhanced tolerance,it seems m... Studies on the cold-responsive genes and cold signaling of woody species drop far behind in comparison to herbaceous plants.Due to similar lignified structure,perennial characteristic,and enhanced tolerance,it seems much easier to find strongly antifreeze genes and obtain effective results in transgenic woody plants.In this study,Ammopiptanthus mongolicus,an evergreen,broadleaf and cold-resist leguminous shrub growing in the desert of Inner Mongolia,was used as a material for low-temperature induced gene isolation.Through differential expression analysis induced by low-temperature,thirteen up-regulated cDNAs were identified.One of them,AmEBP1,(accession number:DQ519359)confers enhanced cold-tolerance to both transgenic E.coli and transgenic Arabidopsis.Results suggest that AmEBP1 can stimulate the synthesis of ribosome and the dephosphyration of the α-subunit of initiation factor 2(eIF2α),and subsequently promote the translation process.By which the transgenic plants obtained increased cold-resistant ability. 展开更多
关键词 Molecular cloning and functional analyses of low-temperature induced genes from Ammopiptanthus mongolicus
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Interfacial Charge Transfer Induced Electronic Property Tuning of MoS_2 by Molecular Functionalization
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作者 Si-Han Zhou Chun-Wei Zhou +3 位作者 Xiang-Dong Yang Yang Li Jian-Qiang Zhong Hong-Ying Mao 《Chinese Physics Letters》 SCIE CAS CSCD 2021年第5期94-98,共5页
The modulation of electrical properties of MoS_2 has attracted extensive research interest because of its potential applications in electronic and optoelectronic devices.Herein,interfacial charge transfer induced elec... The modulation of electrical properties of MoS_2 has attracted extensive research interest because of its potential applications in electronic and optoelectronic devices.Herein,interfacial charge transfer induced electronic property tuning of MoS_2 are investigated by in situ ultraviolet photoelectron spectroscopy and x-ray photoelectron spectroscopy measurements.A downward band-bending of MoS_2-related electronic states along with the decreasing work function,which are induced by the electron transfer from Cs overlayers to MoS_2,is observed after the functionalization of MoS_2 with Cs,leading to n-type doping.Meanwhile,when MoS_2 is modified with 2,3,5,6-tetrafluoro-7,7,8,8-tetracyanoquinodimethane(F_4-TCNQ),an upward band-bending of MoS_2-related electronic states along with the increasing work function is observed at the interfaces.This is attributed to the electron depletion within MoS_2 due to the strong electron withdrawing property of F_4-TCNQ,indicating p-type doping of MoS_2.Our findings reveal that surface transfer doping is an effective approach for electronic property tuning of MoS_2 and paves the way to optimize its performance in electronic and optoelectronic devices. 展开更多
关键词 Interfacial Charge Transfer induced Electronic Property Tuning of MoS_2 by Molecular functionalization
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TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions 被引量:2
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作者 Manoj Muthukuru Richard P Darveau 《Bone Research》 SCIE CAS 2014年第4期218-230,共13页
Toll-like receptor (TLR)-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR si... Toll-like receptor (TLR)-mediated inflammatory response could negatively affect bone metabolism. In this study, we determined how osteogenesis is regulated during inflammatory responses that are downstream of TLR signaling. Human primary osteoblasts were cultured in collagen gels. Pam3CSK4 (P3C) and Escherichia coli lipopolysaccharide (EcLPS) were used as TLR2 and TLR4 ligand respectively. Porphyromonas gingivalis LPS having TLR2 activity with either TLR4 agonism (Pg1690) or TLR4 antagonism (Pg1449) and mutant E. coli LPS (LPxE/LPxF/WSK) were used. IL-lp, SH2-containing inositol phosphatase-1 (SHIP1) that has regulatory roles in osteogenesis, alkaline phosphatase and mineralization were analyzed. 3α-Aminocholestane (3AC) was used to inhibit SHIP1. Our results suggest that osteoblasts stimulated by P3C, poorly induced IL-1β but strongly upregulated SHIP1 and enhanced osteogenic mediators. On the contrary, EcLPS significantly induced IL-1β and osteogenic mediators were not induced. While Pg1690 downmodulated osteogenic mediators, Pg1449 enhanced osteogenic responses, suggesting that TLR4 signaling annuls osteogenesis even with TLR2 activity. Interestingly, mutant E. coli LPS that induces weak inflammation upregulated osteogenesis, but SHIP1 was not induced. Moreover, inhibiting SHIP1 significantly upregulated TLR2-mediated inflammatory response and downmodulated osteogenesis. In conclusion, these results suggest that induction of weak inflammatory response through TLR2 (with SHIP1 activity) and mutant TLR4 ligands could enhance osteogenesis. 展开更多
关键词 TLR signaling that induces weak inflammatory response and SHIP1 enhances osteogenic functions SHIP FIGURE
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In-situ atomic-scale electrically induced ion migration and structural evolution of functional oxides
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《Science Foundation in China》 CAS 2017年第4期17-,共1页
With the support of the National Natural Science Foundation of China,two original studies by the research group led by Prof.Gu Lin(谷林)and Prof.Zhang Qinghua(张庆华)from the Institute of Physics,Chinese Academy of Sc... With the support of the National Natural Science Foundation of China,two original studies by the research group led by Prof.Gu Lin(谷林)and Prof.Zhang Qinghua(张庆华)from the Institute of Physics,Chinese Academy of Sciences demonstrate the in-situ atomic-scale electrically induced 展开更多
关键词 In-situ atomic-scale electrically induced ion migration and structural evolution of functional oxides Nature
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