Isolating cDNA clones from rice induced by Magnaporthe grisea using PCR based differential screening method

The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and time-consuming.Second,the amount of phageDNAs transferred onto the two filters may notbe equivalent,which leads to an inaccurate se-lection of a positive clone.Third,isolation ofphage DNA is slow and cumbersome.Here,aPCR based differential screening method that

Screening-current-induced magnetic fields and strains in a compact REBCO coil in self field and background field

REBa_(2)Cu_(3)O_(7−x)(REBCO)coated conductors,owing to its high tensile strength and current‐carrying ability in a background field,are widely regarded a promising candidate in high‐field applications.Despite the great potentials,recent studies have highlighted the challenges posed by screening currents,which are featured by a highly nonuniform current distribution in the superconducting layer.In this paper,we report a comprehensive study on the behaviors of screening currents in a compact REBCO coil,specifically the screeningcurrent‐induced magnetic fields and strains.Experiments were carried out in the self‐generated magnetic field and a background field,respectively.In the self‐field condition,the full hysteresis of the magnetic field was obtained by applying current sweeps with repeatedly reversed polarity,as the nominal center field reached 9.17 T with a maximum peak current of 350 A.In a background field of 23.15 T,the insert coil generated a center field of 4.17 T with an applied current of 170 A.Ultimately,a total center field of 32.58 T was achieved before quench.Both the sequential model and the coupled model considering the perpendicular field modification due to conductor deformation are applied.The comparative study shows that,for this coil,the electromagnetic–mechanical coupling plays a trivial role in self‐field conditions up to 9 T.In contrast,with a high axial field dominated by the background field,the coupling effect has a stronger influence on the predicted current and strain distributions.Further discussions regarding the role of background field on the strains in the insert suggest potential design strategies to maximize the total center field.

Screening of Epstein-Barr Virus Early Antigen Expression Inducers from Chinese Medicinal Herbs and Plants

Ether extrilcls of 1693 Chinesc medicinal herbs and plilnts from 268 families werestudied for the induction of Epstcin-Barr viral (EBV ) early antigcn (EA ) expression in theRaji cell line. Fifty-two from 18 families were found to have inducing activity. Twenty-fiveand seven of them were from Euphorbiaccae and Thymclaeaceae, respectively. Some ofthem, such as Croton tiglium, Euphorbia kansui, Daphnc genkwa, Wikstrocmia chamacdaphen, Wikstroemia indica, Prunus mandshurica Koehne and Achyranthes bidentata arecommonly used drugs. The significance of these herbs in the activation of EBV in vivo andtheir relation to the development of nasopharyngeal carcinoma were discussed.