The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physi...The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on.Hemp seed disinfection used 75% alcohol for 2 min and sterilized in 1‰ Hg Cl2 for 5min. The best combinations of plant growth regulators were 1.0 mg/L 6-BA and 0.5mg/L NAA for the induction of callus, and the best combinations of hormones were1.0 mg/L KT and 0.5 mg/L NAA for differentiation rate of adventitious bud.展开更多
Industrial hemp (Cannabis sativa) has made a remarkable impact worldwide due to the plant’s beneficial properties and versatile use. Vegetative cuttings (clones) of C. sativa are the preferred propagation technique t...Industrial hemp (Cannabis sativa) has made a remarkable impact worldwide due to the plant’s beneficial properties and versatile use. Vegetative cuttings (clones) of C. sativa are the preferred propagation technique to be the most effective in retaining the same genetic information and reducing hybridization and mutations. The objective of this project was to assess cannabinoid profile concentrations of successively cloned generations of 5 varieties: Cherry, Cherry Blossom, Cherry × Workhorse, Sour Space Candy, and The Wife. This research project focused on the idea that every cloned plant contains the exact same genetic information and, therefore, should have the same metabolic profile of cannabinoids through all the successive generations grown, which is shown to be true. Plants were cloned for multiple generations using stem cuttings and a commercial cloner. As plants matured, they were set in environmental conditions to stimulate flowering and buds were harvested and analyzed for cannabinoid contents using HPLC. Several generations of each variety were successfully cloned. As many as 17 different cannabinoids were analyzed and the results of this study show that there is not a significant difference in cannabinoids over successive generations, showing no major trends.展开更多
Cannabis sativa has a long history of medication,which is often medicated with seeds.Moreover,cannabidiol(CBD),the active ingredient extracted from the flowers and leaves of industrial hemp,has high medicinal value.Wi...Cannabis sativa has a long history of medication,which is often medicated with seeds.Moreover,cannabidiol(CBD),the active ingredient extracted from the flowers and leaves of industrial hemp,has high medicinal value.With the acceptance of CBD by the World Health Organization(WHO)in 2017 and being in the international list as a non-controlled substance,the international strict policy on industrial hemp has been slowly liberalized.It has been proved that the industrial hemp has great economic value and application potential in many aspects.In order to make better use of the unique efficacy of industrial hemp,the definition of industrial hemp and its current laws in the domestic and overseas are systematically studied and summarized in this paper.At the same time,the research status of hemp seed oil and hemp leaves extractive in cosmetics is expounded,which is helpful to the comprehensive development and application of industrial hemp.展开更多
Though there are many industries where China now commands a leading presence,cannabis production probably isn’t the first that comes to mind.The South China Morning Post recently shone a light on the relatively low-k...Though there are many industries where China now commands a leading presence,cannabis production probably isn’t the first that comes to mind.The South China Morning Post recently shone a light on the relatively low-key hemp industry,and how China now accounts for approximately half of the world’s legal展开更多
基金Supported by Heilongjiang Academy of Sciences Youth Innovation Fund~~
文摘The internodes of the new cultivar Long-ma No.1 of Cannabis sativa L.（Industrial hemp） were used as explants for tissue culture. The paper studied the key factors of industrial hemp tissue culture, such as the physiological state of aseptic seedlings, the selection and concentration of plant growth regulators and so on.Hemp seed disinfection used 75% alcohol for 2 min and sterilized in 1‰ Hg Cl2 for 5min. The best combinations of plant growth regulators were 1.0 mg/L 6-BA and 0.5mg/L NAA for the induction of callus, and the best combinations of hormones were1.0 mg/L KT and 0.5 mg/L NAA for differentiation rate of adventitious bud.
文摘Industrial hemp (Cannabis sativa) has made a remarkable impact worldwide due to the plant’s beneficial properties and versatile use. Vegetative cuttings (clones) of C. sativa are the preferred propagation technique to be the most effective in retaining the same genetic information and reducing hybridization and mutations. The objective of this project was to assess cannabinoid profile concentrations of successively cloned generations of 5 varieties: Cherry, Cherry Blossom, Cherry × Workhorse, Sour Space Candy, and The Wife. This research project focused on the idea that every cloned plant contains the exact same genetic information and, therefore, should have the same metabolic profile of cannabinoids through all the successive generations grown, which is shown to be true. Plants were cloned for multiple generations using stem cuttings and a commercial cloner. As plants matured, they were set in environmental conditions to stimulate flowering and buds were harvested and analyzed for cannabinoid contents using HPLC. Several generations of each variety were successfully cloned. As many as 17 different cannabinoids were analyzed and the results of this study show that there is not a significant difference in cannabinoids over successive generations, showing no major trends.
文摘Cannabis sativa has a long history of medication,which is often medicated with seeds.Moreover,cannabidiol(CBD),the active ingredient extracted from the flowers and leaves of industrial hemp,has high medicinal value.With the acceptance of CBD by the World Health Organization(WHO)in 2017 and being in the international list as a non-controlled substance,the international strict policy on industrial hemp has been slowly liberalized.It has been proved that the industrial hemp has great economic value and application potential in many aspects.In order to make better use of the unique efficacy of industrial hemp,the definition of industrial hemp and its current laws in the domestic and overseas are systematically studied and summarized in this paper.At the same time,the research status of hemp seed oil and hemp leaves extractive in cosmetics is expounded,which is helpful to the comprehensive development and application of industrial hemp.
文摘Though there are many industries where China now commands a leading presence,cannabis production probably isn’t the first that comes to mind.The South China Morning Post recently shone a light on the relatively low-key hemp industry,and how China now accounts for approximately half of the world’s legal
