Eyelid plays a vital role in protecting the eye from injury or infection.Inflammation related eyelid diseases,such as blepharitis,are the most common ocular disorders that affect human’s vision and quality of life.Du...Eyelid plays a vital role in protecting the eye from injury or infection.Inflammation related eyelid diseases,such as blepharitis,are the most common ocular disorders that affect human’s vision and quality of life.Due to the physiological barriers and anatomical structures of the eye,the bioavailability of topical administrated therapeutics is typically less than 5%.Herein,we developed a bio-responsive hydrogel drug delivery system using a generally recognized as safe compound,triglycerol monostearate(TG-18),for in-situ eyelid injection with sustained therapeutics release.In vitro,drug release and disassembly time of Rosiglitazone loaded hydrogel(Rosi-hydrogel)were estimated in the presence or absence of MMP-9,respectively.Moreover,the disassembly of TG-18 hydrogel was evaluated with 9-month-old and 12-month-old mice in vivo.Owing to the bio-responsive nature of Rosi-hydrogel,the on-demand Rosiglitazone release is achieved in response to local enzymes.These findings are proved by further evaluation in the age-related meibomian gland dysfunction mice model,and the bio-responsive hydrogel is used as an in-situ injection to treat eyelid diseases.Taken together,the in-situ eyelid injection with sustained drug release opens a window for the therapy of inflammation related eyelid diseases.展开更多
Inflammation is a common disease involved in the pathogenesis,complications,and sequelae of a large number of related diseases,and therefore considerable research has been directed toward developing anti-inflammatory ...Inflammation is a common disease involved in the pathogenesis,complications,and sequelae of a large number of related diseases,and therefore considerable research has been directed toward developing anti-inflammatory drugs for the prevention and treatment of these diseases.Traditional Chinese medicine(TCM)has been used to treat inflammatory and related diseases since ancient times.According to the re-view of abundant modern scientific researches,it is suggested that TCM exhibit anti-inflammatory effects at different levels,and via multiple pathways with various targets,and recently a series of in vitro and in vivo anti-inflammatory models have been developed for anti-inflammation research in TCM.Currently,the reported classic mechanisms of TCM and experimental models of its anti-inflammatory effects pro-vide reference points and guidance for further research and development of TCM.Importantly,the research clearly confirms that TCM is now and will continue to be an effective form of treatment for many types of inflammation and inflammation-related diseases.展开更多
基金This study was supported by the National Natural Science Foundation of China(81570812,81770888)the Shanghai Municipal Education Commission:Gaofeng Clinical Medicine Grant Support(20161421)+1 种基金“Two-hundred”Talent(20191820)the Science and Technology Commission of Shanghai(20DZ2270800).
文摘Eyelid plays a vital role in protecting the eye from injury or infection.Inflammation related eyelid diseases,such as blepharitis,are the most common ocular disorders that affect human’s vision and quality of life.Due to the physiological barriers and anatomical structures of the eye,the bioavailability of topical administrated therapeutics is typically less than 5%.Herein,we developed a bio-responsive hydrogel drug delivery system using a generally recognized as safe compound,triglycerol monostearate(TG-18),for in-situ eyelid injection with sustained therapeutics release.In vitro,drug release and disassembly time of Rosiglitazone loaded hydrogel(Rosi-hydrogel)were estimated in the presence or absence of MMP-9,respectively.Moreover,the disassembly of TG-18 hydrogel was evaluated with 9-month-old and 12-month-old mice in vivo.Owing to the bio-responsive nature of Rosi-hydrogel,the on-demand Rosiglitazone release is achieved in response to local enzymes.These findings are proved by further evaluation in the age-related meibomian gland dysfunction mice model,and the bio-responsive hydrogel is used as an in-situ injection to treat eyelid diseases.Taken together,the in-situ eyelid injection with sustained drug release opens a window for the therapy of inflammation related eyelid diseases.
基金supported by the China Postdoctoral Science Foundation (no. 2020M670599)
文摘Inflammation is a common disease involved in the pathogenesis,complications,and sequelae of a large number of related diseases,and therefore considerable research has been directed toward developing anti-inflammatory drugs for the prevention and treatment of these diseases.Traditional Chinese medicine(TCM)has been used to treat inflammatory and related diseases since ancient times.According to the re-view of abundant modern scientific researches,it is suggested that TCM exhibit anti-inflammatory effects at different levels,and via multiple pathways with various targets,and recently a series of in vitro and in vivo anti-inflammatory models have been developed for anti-inflammation research in TCM.Currently,the reported classic mechanisms of TCM and experimental models of its anti-inflammatory effects pro-vide reference points and guidance for further research and development of TCM.Importantly,the research clearly confirms that TCM is now and will continue to be an effective form of treatment for many types of inflammation and inflammation-related diseases.
