Fluorescent in situ hybridization (FISH) was applied to investigate the phylogenetic relationship among Dendranthema spp. Genomic DNA of wild species which was used as probe did not give specific signals, while 18S-26...Fluorescent in situ hybridization (FISH) was applied to investigate the phylogenetic relationship among Dendranthema spp. Genomic DNA of wild species which was used as probe did not give specific signals, while 18S-26S rDNA from Arabidopsis, which was used as control probe, showed the loci on the target chromosomes clearly. Satisfied results of FISH were gotten when denaturing digoxingenen-labeled probe and chromosome together in oven at 80℃ for 1015min. There is little influence on the result by the stringency of washing when rDNA was used as probe. The result also indicates the limitation of genomic in situ hybridization (GISH) when used as an approach to analyze the phylogenetic relationship among Dendranthema spp. and the origin of cultivated chrysanthemum.展开更多
As the significance of the gut microbiota has become increasingly realized,a large number of related studies have emerged.With respect to the gut microbial composition of fish,the predominant gut microbes and core gut...As the significance of the gut microbiota has become increasingly realized,a large number of related studies have emerged.With respect to the gut microbial composition of fish,the predominant gut microbes and core gut microbiota have been reported by many researchers.Our understanding of fish gut microbiota,especially its functional roles,has fallen far behind that of terrestrial vertebrates,although previous studies using gnotobiotic zebrafish models have revealed that the gut microbiota performs a significant role in gut development,nutrient metabolism and immune responses.Given that environmental factors of marine habitats are very different from those of freshwater habitats,a distinct difference may exist in the gut microbiota between freshwater and marine fish.Therefore,this review aims to address the advances in marine fish gut microbiota in terms of methodologies,the gut microbial composition,and gnotobiotic models of marine fish,the important factors(host genotype and three environmental factors:temperature,salinity and diet)that drive marine fish gut microbiota,and significant roles of the gut microbiota in marine fish.展开更多
基金National Natural Science Foundation of China (Grant No. 39600119)
文摘Fluorescent in situ hybridization (FISH) was applied to investigate the phylogenetic relationship among Dendranthema spp. Genomic DNA of wild species which was used as probe did not give specific signals, while 18S-26S rDNA from Arabidopsis, which was used as control probe, showed the loci on the target chromosomes clearly. Satisfied results of FISH were gotten when denaturing digoxingenen-labeled probe and chromosome together in oven at 80℃ for 1015min. There is little influence on the result by the stringency of washing when rDNA was used as probe. The result also indicates the limitation of genomic in situ hybridization (GISH) when used as an approach to analyze the phylogenetic relationship among Dendranthema spp. and the origin of cultivated chrysanthemum.
基金This work was financially supported by National Key R&D Program of China(No.2018YFD0900400)National Natural Science Foundation of China(Nos.31872577,41576137)China Agriculture Researches System(Grant No.CARS 47-G10).
文摘As the significance of the gut microbiota has become increasingly realized,a large number of related studies have emerged.With respect to the gut microbial composition of fish,the predominant gut microbes and core gut microbiota have been reported by many researchers.Our understanding of fish gut microbiota,especially its functional roles,has fallen far behind that of terrestrial vertebrates,although previous studies using gnotobiotic zebrafish models have revealed that the gut microbiota performs a significant role in gut development,nutrient metabolism and immune responses.Given that environmental factors of marine habitats are very different from those of freshwater habitats,a distinct difference may exist in the gut microbiota between freshwater and marine fish.Therefore,this review aims to address the advances in marine fish gut microbiota in terms of methodologies,the gut microbial composition,and gnotobiotic models of marine fish,the important factors(host genotype and three environmental factors:temperature,salinity and diet)that drive marine fish gut microbiota,and significant roles of the gut microbiota in marine fish.
