High-starchy carbohydrate diet aggravates NAFLD by increasing fatty acids influx mediated by NOX2

Nonalcoholic fatty liver disease(NAFLD)is a high-incidence lipid disorder that affects more than a quarter of the population worldwide,and dietary intervention is the recognized treatment.Starch is the main component of staple foods that are consumed daily,and the effects,metabolic pathway,and molecular mechanism of starch in the context of NAFLD remain unclear.Our study showed that a high-starch carbohydrate diet(HCD)led to the occurrence and exacerbation of NAFLD in mice.Transcriptomics and metabonomic analyses showed that the increased fatty acid influx mediated by NADPH oxidase 2(NOX2)exacerbated NAFLD.Knocking down NOX2 specifically alleviated HCD-induced NAFLD in vivo and in vitro.Moreover,the large amounts of ROS produced by NOX2 further exacerbated insulin resistance and increased lipolysis in perirenal white adipose tissue(periWAT),thereby providing fatty acids for hepatic lipid synthesis.In addition,the interaction between AMPKα1 and p47phox was the pathway that mediated the high expression of NOX2 induced by a HCD.Our study systematically demonstrated the effect of a HCD on NAFLD.Elevated fatty acid influx is a unique molecular regulatory pathway that mediates HCD-induced NAFLD exacerbation,which is different from the effect of simple sugars.Additionally,NOX2 was suggested to be a specific and effective drug target for NAFLD.

Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca^(2+)

Jujube contains abundant cyclic adenosine monophosphate(cAMP)and the ultrasonic-assisted pectinase extraction(UAPE)conditions for obtaining the maximum cAMP yield from jujube were optimized.Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield.The results showed that the optimal cAMP yield(783.0μg/g)was derived at ratio of liquid to solid 5 mL/g,ratio of pectinase to raw material 1.5%,time 60 min and temperature 40℃.Moreover,the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E(IgE)and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia(RBL-2 H3)cells using dinitrophenylated(DNP)-bovine serum albumin(BSA)-IgE.The results showed that cAMP interfered with sensitized cells,effectively inhibited the occurrence of basophil degranulation in dose dependence,and significantly reduced the activity ofβ-hexosamindase(β-hex),at the optimal concentration of 50μg/mL.The level of anti-inflammatory factor interleukin-10(IL-10)was promoted and the content of pro-inflammatory factor tumor necrosis factor-α(TNF-α)was suppressed by cAMP.In addition,influx of intracellular Ca^(2+) was repressed effectively.Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca^(2+),with the prevention of allergy symptoms.

对FETKOVICH(费特科维奇)典型曲线的质疑与评论

FETKOVICH(费特科维奇,以下简称为费氏)于1971年和1980年,分别提出的有限水域水侵量方程和定压典型曲线,受到国内外专家的重视和引用。由于费氏典型曲线可以通过实际数据的拟合,确定井的驱动半径和驱动面积,因此,受到业内专家的青睐。通过推导表明,费氏有限水域水侵量方程,是一个指数递减方程。费氏将该方程直接应用于定容封闭边界油井的产量递减分析,并基于初始递减率的关系式,得到了费氏典型曲线的无因次时间。费氏利用无因次压力的倒数作为无因次产量,得到了典型曲线的无因次产量。然而,由于费氏典型曲线的无因次时间和无因次产量之间没有直接的函数关系,无法建立费氏的无因次典型曲线,因此,对费氏的有限水域水侵量方程和典型曲线的无因次时间和无因次产量进行了推导,并对存在的问题提出了质疑和评论。

底水油藏水侵量计算新方法

针对底水油藏的开发,在缺乏试井数据的情况下采用常规油藏物质平衡法以及试井分析方法均难以确定水驱控制储量,进而无法获得水体参数和特征来计算油田的水侵量。为了更加准确的预测水驱油田水侵量,结合生产动态资料,选择符合的水驱特征曲线计算水驱控制储量。在此基础上,考虑油环油、溶解气、水体等因素,建立物质平衡方程,通过亏空体积曲线法计算获得水侵量。利用Fetkvoitch拟稳态模型,采用试错法,优化计算水体体积和水侵系数,保证Fetkvoitch拟稳态模型求解的水侵量和亏空体积曲线法求解的水侵量最接近,得到最优的水体体积和水侵系数对未来生产动态中水侵量进行预测。运用油藏数值模拟软件建立的模型与本文提出两种的计算方法拟合结果对比可知的水体体积的误差为2.6%、水侵指数的误差为2.2%,计算结果准确,为底水油藏计算水侵量提供了可靠的依据,并为预测水侵量提供了新的方法。

High-frequency repetitive transcranial magnetic stimulation promotes neural stem cell proliferation after ischemic stroke

Prolife ration of neural stem cells is crucial for promoting neuronal regeneration and repairing cerebral infarction damage.Transcranial magnetic stimulation(TMS)has recently emerged as a tool for inducing endogenous neural stem cell regeneration,but its underlying mechanisms remain unclea r In this study,we found that repetitive TMS effectively promotes the proliferation of oxygen-glucose deprived neural stem cells.Additionally,repetitive TMS reduced the volume of cerebral infa rction in a rat model of ischemic stro ke caused by middle cerebral artery occlusion,im p roved rat cognitive function,and promoted the proliferation of neural stem cells in the ischemic penumbra.RNA-sequencing found that repetitive TMS activated the Wnt signaling pathway in the ischemic penumbra of rats with cerebral ischemia.Furthermore,PCR analysis revealed that repetitive TMS promoted AKT phosphorylation,leading to an increase in mRNA levels of cell cycle-related proteins such as Cdk2 and Cdk4.This effect was also associated with activation of the glycogen synthase kinase 3β/β-catenin signaling pathway,which ultimately promotes the prolife ration of neural stem cells.Subsequently,we validated the effect of repetitive TMS on AKT phosphorylation.We found that repetitive TMS promoted Ca2+influx into neural stem cells by activating the P2 calcium channel/calmodulin pathway,thereby promoting AKT phosphorylation and activating the glycogen synthase kinase 3β/β-catenin pathway.These findings indicate that repetitive TMS can promote the proliferation of endogenous neural stem cells through a Ca2+influx-dependent phosphorylated AKT/glycogen synthase kinase 3β/β-catenin signaling pathway.This study has produced pioneering res ults on the intrinsic mechanism of repetitive TMS to promote neural function recove ry after ischemic stro ke.These results provide a stro ng scientific foundation for the clinical application of repetitive TMS.Moreover,repetitive TMS treatment may not only be an efficient and potential approach to support neurogenesis for further therapeutic applications,but also provide an effective platform for the expansion of neural stem cells.

卸压增透下底板定向拦截钻孔瓦斯抽采模拟研究

杜儿坪矿68311工作面在回采时下邻近层瓦斯涌入量较多,为此采用在底板打定向拦截钻孔并进行瓦斯抽采的方法。为体现工作面回采对底板造成的卸压增透以及合理设计瓦斯抽采参数,首先使用FLAC3D模拟工作面回采不同距离下的底板有效应力变化,将其转换为渗透率变化并导入至COMSOL中,再通过建立流-固耦合数学模型完成不同抽采参数下的瓦斯抽采模拟。研究结果表明:工作面回采距离越大,卸压增透效果越好;钻孔层位由6 m降低至2 m,瓦斯抽采有效半径提高15.10%;钻孔直径由φ60 mm增加至φ120 mm,瓦斯抽采有效半径增加15.03%。

辽东湾北部河流氮磷入海通量及污染源解析

为量化陆源氮磷污染对辽东湾北部海洋环境的影响,以辽东湾北部的浑河和太子河流域(浑太流域)为研究对象,通过构建SWAT模型,对2015—2018年陆源氮磷污染负荷的产生量以及入海通量进行模拟,分析其年际和年内变化特征,并探究氮磷污染的来源、贡献率及驱动因素。结果表明:陆源氮磷污染产生量及入海通量整体呈减少趋势(2016年除外),太子河氮磷通量约为浑河的2.4倍;受降水驱动影响,丰水期总磷及氨氮的入海通量占比均超过55%。总磷及氨氮在入海污染中贡献率较高,其入海通量与污染物浓度的相关性远低于入海流量。因此,从源头上加强浑太流域污染治理,减少氮磷的汇入,是解决辽东湾北部海洋污染问题的关键。

基于水驱特征曲线的水驱气藏水侵量计算

针对水驱气藏水侵量计算过程复杂的问题,提出通过建立水驱物质平衡方程与水驱特征曲线之间的关系来求解水侵量的方法。首先从气水两相渗流规律及地层流体产能方程出发,得到水驱气藏的水驱特征曲线,即气井见水后的阶段累产气量与水气比的自然对数呈线性关系,通过斜率即可求解气井见水后的动态储量,加上气井见水前的累产气量作为总的动态储量,结合水驱物质平衡方程,可以计算出水驱气藏的水侵量。实例应用表明,水侵量计算结果在地质气藏模型中能很好地吻合上生产历史数据,证明该方法具有较强的实用性,能够为水驱气藏动态评价与治水措施的选择提供依据。

巷道掘进过程中涌水灾害预注浆治理研究

随着某矿山开采深度的增加,矿井巷道面临涌水灾害的严峻挑战,对施工安全和生产效率构成了严重的影响。针对该问题,研究通过深入分析,确定涌水的主因系灰岩与隔水层接触界面的水溶蚀作用及地表降水的补给。根据工程地质和水文地质条件,设计了一套预注浆方案,包括注浆方式的选择、加固厚度的确定、注浆材料的选用、浆液扩散半径、终孔间距和注浆段长度的计算。在注浆孔布置、注浆液配制和注浆施工流程方面进行了详细的设计和规划。实际施工中,采用了双液注浆技术,并根据地层吸浆能力动态调整注浆参数。治理结果显示,超前预注浆技术显著减小了涌水量,从280 m^(3)/h减小至50 m^(3)/h,减幅达到82.1%,有效确保了巷道的安全与稳定,为类似矿山施工中的涌水问题提供了借鉴和指导。

Mathematical modeling of heat transfer in counter-current multiphase flow found in gas-drilling systems with formation fluid influx

The performance of gas-drilling(drilling oil and gas wells with air, nitrogen, or natural gas) is very unpredictable in many areas due to lack of proper design of drilling parameters because of limited understanding of gas–rock interaction which requires knowledge of heat transfer in the well system. Complete analysis of rock failure requires an accurate mathematical model to predict gas temperature at the bottom hole. The currently available mathematical models are unsuitable for use for the purpose because they do not consider the effects of formation fluid influx, Joule–Thomson cooling, and entrained drill cuttings. A new analytical solution for predicting gas temperature profiles inside the drill string and in the annulus was derived in this study for gas-drilling, considering all these three effects. Results of sensitivity analyses show that formation fluid influx can significantly increase the temperature profiles in both the drill string and the annulus.The Joule–Thomson cooling effect lowers the temperature in the annulus only in a short interval near the bottom hole.The drill cuttings entrained at the bottom hole can slightly increase the temperature profile in the annulus.

Photosynthesis-Dependent Extracellular Ca2+ Influx Triggers an Asexual Reproductive Cycle in the Marine Red Macroalga Porphyra yezoensis

Asexual propagation to increase the number of gametophytic clones via the growth of asexual haploid spores is a unique survival strategy found in marine multicellular algae. However, the mechanisms regulating the asexual life cycle are largely unknown. Here, factors involved in the regulation of production and discharge of asexual spores, so-called monospores, are identified in the marine red macroalga Porphyra yezoensis. First, enhanced discharge of monospores was found by incubation of gametophytes in ASPMT1, a modified version of the previously established synthetic medium ASP12. Comparison of the compositions of ASPMT1 and our standard medium, ESL, indicated that the Ca2+ concentration in ASPMT1 was three times lower than that in ESL medium. Thus, we modified ASPMT1 by increasing its Ca2+ concentration, resulting in reduction of monospore discharge. These findings demonstrate the role of reduced Ca2+ concentrations in enhancing monospore production and release. Moreover, it was also observed that initiation of asexual life cycle required illumination, was repressed by DCMU, and was induced by a Ca2+ ionophore in the dark. Taken together, these results indicate that photosynthesis-dependent Ca2+ influx triggers the asexual life cycle by promoting the production and discharge of monospores in P. yezoensis.

Glycolytic potential enhanced by blockade of pyruvate influx into mitochondria sensitizes prostate cancer to detection and radiotherapy

Objective:This study aimed to evaluate the effects of mitochondrial pyruvate carrier(MPC)blockade on the sensitivity of detection and radiotherapy of prostate cancer(PCa).Methods:We investigated glycolysis reprogramming and MPC changes in patients with PCa by using metabolic profiling,RNASeq,and tissue microarrays.Transient blockade of pyruvate influx into mitochondria was observed in cellular studies to detect its different effects on prostate carcinoma cells and benign prostate cells.Xenograft mouse models were injected with an MPC inhibitor to evaluate the sensitivity of 18F-fluorodeoxyglucose positron emission tomography with computed tomography and radiotherapy of PCa.Furthermore,the molecular mechanism of this different effect of transient blockage towards benign prostate cells and prostate cancer cells was studied in vitro.Results:MPC was elevated in PCa tissue compared with benign prostate tissue,but decreased during cancer progression.The transient blockade increased PCa cell proliferation while decreasing benign prostate cell proliferation,thus increasing the sensitivity of PCa cells to 18F-PET/CT(SUVavg,P=0.016;SUVmax,P=0.03)and radiotherapy(P<0.01).This differential effect of MPC on PCa and benign prostate cells was dependent on regulation by a VDAC1-MPC-mitochondrial homeostasis-glycolysis pathway.Conclusions:Blockade of pyruvate influx into mitochondria increased glycolysis levels in PCa but not in non-carcinoma prostate tissue.This transient blockage sensitized PCa to both detection and radiotherapy,thus indicating that glycolytic potential is a novel mechanism underlying PCa progression.The change in the mitochondrial pyruvate influx caused by transient MPC blockade provides a critical target for PCa diagnosis and treatment.

Optimization of Evidence Analysis Cost Using Arbitrary Re-Sampling Techniques for Sample Influx into Forensic Science Laboratory

This study analyzes the sample influx (samples per case file) into forensic science laboratory (FSL) and the corresponding analysis costs and uses arbitrary re-sampling plans to establish the minimum cost function. The demand for forensic analysis increased for all disciplines, especially biology/DNA between 2014 and 2015. While the average distribution of case files was about 42.5%, 40.6% and 17% for the three disciplines, the distribution of samples was rather different being 12%, 82.5% and 5.5% for samples requiring forensic biology, chemistry and toxicology analysis, respectively. Results show that most of the analysis workload was on forensic chemistry analysis. The cost of analysis for case files and the corresponding sample influx varied in the ratio of 35:6:1 and 28:12:1 for forensic chemistry, biology/DNA and toxicology for year 2014 for 2015, respectively. In the two consecutive years, the cost for forensic chemistry analysis was comparatively very high, necessitating re-sampling. The time series of sample influx in all disciplines are strongly stochastic, with higher magnitude for chemistry, biology/DNA and toxicology, in this order. The PDFs of sample influx data are highly skewed to the right, especially forensic toxicology and biology/DNA with peaks at 1 and 3 samples per case file. The arbitrary re-sampling plans were best suited to forensic chemistry case files (where re-sampling conditions apply). The locus of arbitrary number of samples to take from the submitted forensic samples was used to establish the minimum and scientifically acceptable samples by applying minimization function developed in this paper. The cost minimization function was also developed based on the average cost per sample and choice of re-sampling plans depending on the range of sample influx, from which the savings were determined and maximized. Thus, the study gives a forensic scientist a business model and scientific decision making tool on minimum number of samples to analyze focusing on savings on analysis cost.

Spectroscopic Measurement of Neutral Particle Influx Ratio on EAST

The spectra of HeI（587.6 nm）,Hα（656.28 nm） and Dα（656.1 nm） of the helium discharges as well as the normal deuterium discharges have been measured with two optical spectroscopic multi-channel analysis（OMA） systems on the experimental advanced superconducting tokamak（EAST）.The influx ratio of the sum of H and D to He spectral lines and the influx ratio of H to D are given.In this way the ratio of hydrogen/deuterium ion（S/X B）H/D to（S/X B）He as well as（S/X B）H/D is not very sensitive to the variation in the edge density and temperature. The low-density helium discharges are operated in order to reduce the recycling hydrogen fluxes; however,the effect is not obvious.The possible reason is that the number of helium discharges is not enough and the content of hydrogen in the wall is still very abundant,which is caused by frequent wall conditionings and the vacuum leakage.The H/（H＋D） ratio decreases quickly after one lithium coating and reduces to less than 10%using several accumulated lithium wall conditioning.It is found that the deposited He atoms on the carbon wall will remain at a low level after several D2 discharges.

Hypericum caprifoliatum and Hypericum connatum affect human trophoblast-like cells differentiation and Ca^(2+) influx

Objective:To study the effect of crude methanol and n-hexane extracts of Hypericum connatum(H.connatum) and Hypericum caprifoliatum on trophoblast-like cells.Methods:BeWo and JEG-3 trophoblast-like cells were submitted to different extract concentrations(1.5,10 and 15 μg/mL) and evaluated in relation to cell viability and in titro trophoblast differentiation and function.Cell viability was evaluated using WST-1 reagent.Differentiation was measured by luciferase production,hCG production/release,and mitogenactivated protein kinase signaling pathway activation.The function of the trophoblast-like cells was measured by^(25)Ca^(2*) influx evaluation.Results:The results showed a decrease in cell viability/proliferation.Both plants and different extracts induced a significant decrease in hCG production/release and luciferase production.H.connatum did not cause mitogen-activated protein kinase signaling pathway disturbance;however,Hypericum caprifoliatum n-hexane extract at 15 μg/mL inhibited extracellular signalregulated kinase 1/2 activation.The significant increase in Ca^(2+) influx by JEG-3 cells was seen after short and long incubation times with H.connatum methanolie extract at 15 μg/mL.Conclusions:The results indicated that these two Hypericum species extracts can interfere on trophoblast differentiation and Ca^(2+)influx,according to their molecular diversity.Although in vivo experiments are necessary to establish their action on placental formation and function,this study suggests that attention must be paid to the potential toxic effect of these plants.

Attenuation of nicotine-evoked Ca²⁺influx by antibody to the nicotinic acetylcholine receptor <i>α</i>3 subunits in human embryonic kidney cells

Autoantibody against neuronal nicotinic acetylcholine receptor (nAChR) α3 subunit is implicated in severe autonomic dysfunction in the patients with autoimmune autonomic ganglionopathy (AAG). Although this autoantibody has been revealed to impair fast excitatory synaptic transmission in autonomic ganglia, its precise mechanism remains unknown. Here, we show that antibody-induced reduction of cell-surface α3 subunits result in impairment of nicotine-evoked Ca2+ influx in stably transfected human embryonic kidney cells. These effects of the antibody were remarkably inhibited by interfering with the endocytic machinery at low-temperature. We conclude that reduction of nAChR in autonomic ganglia can be mediated by the endocytosis of α3 subunits, and resulted in autonomic failure in AAG patients.

EFFECT OF VERAPAMIL ONCa^（2＋） INFLUX ANDCVB3－RNA REPLICATION IN CULTURED NEONATALRAT HEART CELLS INFECTED WITH CVB3

The effect of verapamil on Ca2+ influx across the myocardial plasma membrane and coxsackie virus B3 ( CVB3)-RNA replication in cultured neonatal rat heart cells infected with CVB3 was investigated. It was found that the Ca2+ influx could be inhibited significantly (P<O. 01) by verapamil (1 μmol/L) after infection of heart cells for 48h. However, when the cultured heart cells infected with CVB3 and treated with verapamil (Iμmol/L and 10 nmo/L) at the same time for 48h, the amounts of CVB3-RNA in myocytes were significantly higher than that in infected control group (P<O. 05). These phenomena suggest that the increase of Ca2+ influx of cultured heart cells infected with CVB3 could be inhibited by some calcium antagonists, e. g. verapamil at the early stage. On the other hand, verapamil might accelerate viral replication in myocardium. Thus, although verapamil could be beneficial for decreasing the secondary Ca2+ damages and improve the myocardial electric activity, it isn’t a sensible choice for therapy in early stage of virus infection with cardiac symptoms.

Nodulin 26-like intrinsic protein Cs NIP2;2 is a silicon influx transporter in Cucumis sativus L.

Nodulin 26-like intrinsic proteins(NIPs) are a family of channel-forming transmembrane proteins that function in the transport of water and other small molecules.Some NIPs can mediate silicon transport across plasma membranes and lead to silicon accumulation in plants,which is beneficial for the growth and development of plants.Cucumber is one of the most widely consumed vegetables;however,the functions of NIPs in this crop are still largely unknown.Here,we report the functional characteristics of Cs NIP2;2.It was found that Cs NIP2;2 is a tandem repeat of Cs NIP2;1,which had been demonstrated to be a silicon influx transporter gene.Cs NIP2;2 has a selectivity filter composed of cysteine,serine,glycine and arginine(CSGR),which is different from all previously characterized silicon influx transporters in higher plants at the second helix position.Xenopus laevis oocytes injected with Cs NIP2;2 c RNA demonstrated a higher uptake of silicon than the control,and the uptake remained unchanged under low temperature.Cs NIP2;2 was found to be expressed in the root,stem,lamina and petiole,and exogenous silicon treatment decreased its expression in the stem but not in other tissues.Transient expression of Cs NIP2;2-e GFP fusion sequence in onion epidermal cells showed that Cs NIP2;2 was localized to the cell nucleus,plasma membrane and an unknown structure inside the cell.The results suggest that Cs NIP2;2 is a silicon influx transporter in cucumber,and its subcellular localization and the selectivity filter are different from those of the previously characterized silicon influx transporters in other plants.These findings may be helpful for understanding the functions of NIPs in cucumber plants.

Analysis of the Effect of Subgroup Size on the X-Bar Control Chart Using Forensic Science Laboratory Sample Influx Data

This paper analyzes the effect of subgroup size on the x-bar chart characteristics using sample influx (SIF) into forensic science laboratory (FSL). The characteristics studied include changes in out-or-control points (OCP), upper control limit UCLx, and zonal demarcations. Multi-rules were used to identify the number of out-of-control-points, Nocp as violations using five control chart rules applied separately. A sensitivity analysis on the Nocp was applied for subgroup size, k, and number of sigma above the mean value to determine the upper control limit, UCLx. A computer code was implemented using a FORTRAN code to create x-bar control-charts and capture OCP and other control-chart characteristics with increasing k from 2 to 25. For each value of k, a complete series of average values, Q(p), of specific length, Nsg, was created from which statistical analysis was conducted and compared to the original SIF data, S(t). The variation of number of out-of-control points or violations, Nocp, for different control-charts rules with increasing k was determined to follow a decaying exponential function, Nocp = Ae–α, for which, the goodness of fit was established, and the R2 value approached unity for Rule #4 and #5 only. The goodness of fit was established to be the new criteria for rational subgroup-size range, for Rules #5 and #4 only, which involve a count of 6 consecutive points decreasing and 8 consecutive points above the selected control limit (σ/3 above the grand mean), respectively. Using this criterion, the rational subgroup range was established to be 4 ≤ k ≤ 20 for the two x-bar control chart rules.

Easy Determination of Water Influx in Reservoirs

This p aper presents two methods for easy determination of water influx in reservoirs based on the material balance theory of reservoirs,i.e.,the Net Production V olume Curve,and the Production Index Curve.These methods can be used for esti-ma tion of water influx in reservoirs without any assumption of aquifer shape and size by applying the production performance data.Hence the tedium of the c onventional trial-and-error calculations and obvious uncertainty could be over come in ap plication of the easy and practical new methods.