BRIGHT blue sky, a fleet of diplomats＇ cars outside, flocks of journalists inside, a memorial site in the size of a small town： right next to the Lugou Bridge - better known in the West as the Marco Polo Bridge - is...BRIGHT blue sky, a fleet of diplomats＇ cars outside, flocks of journalists inside, a memorial site in the size of a small town： right next to the Lugou Bridge - better known in the West as the Marco Polo Bridge - is the location of the Museum of the War of Chinese People＇s Resistance Against Japanese Aggression.展开更多
供热负荷预测是指导供热系统调控的重要手段。提高供热负荷预测精度十分重要,针对机器学习中输出目标的分解预测,提出了一种基于季节和趋势分解(seasonal and trend decomposition using loess,STL)的供热负荷预测方法,构建了适用于供...供热负荷预测是指导供热系统调控的重要手段。提高供热负荷预测精度十分重要,针对机器学习中输出目标的分解预测,提出了一种基于季节和趋势分解(seasonal and trend decomposition using loess,STL)的供热负荷预测方法,构建了适用于供热负荷预测的输出目标。首先利用STL算法将供热负荷时间序列数据分解为趋势分量、周期分量和残差分量,分别训练Informer、BiLSTM和XGB模型,将构建好的3个分量预测模型的输出叠加作为初步预测结果,分析误差序列,以BiLSTM预测误差提高模型精度,构建出STL-Informer-BiLSTM-XGB预测模型。将上述模型与常用预测模型进行对比,结果表明所构建的STL-Informer-BiLSTM-XGB模型的MAPE、MAE和MSE分别为0.871%、96.18和13202.2,预测效果最优,验证了所提出的方法具有较高的供热负荷预测精度。展开更多
随着隔震技术的推广应用以及建筑业信息化水平的持续提升,在隔震工程中对隔震层建筑信息模型(building information modeling, BIM)建模的需求逐渐增长,然而针对性的研究工作相对较少。为此,围绕隔震支座BIM模型的高效建模方法和应用模...随着隔震技术的推广应用以及建筑业信息化水平的持续提升,在隔震工程中对隔震层建筑信息模型(building information modeling, BIM)建模的需求逐渐增长,然而针对性的研究工作相对较少。为此,围绕隔震支座BIM模型的高效建模方法和应用模块开展了研究。首先,综合隔震支座应用情况和力学特性,可将其分为橡胶隔震支座、滑移摩擦隔震支座和其他类型隔震支座,据此提出了隔震支座BIM快速建模模块基本架构;随后,基于Revit和Visual Studio平台开发了三类隔震支座BIM模型的快速建模功能,并实现了连接节点参数化建模和支座批量/手动布置的操作功能;最后,开展了某化工公司的库房隔震加固项目的隔震层BIM模型建模实践,结果表明:利用快速建模模块可将隔震层BIM建模操作从7个步骤降低至2个步骤,且使用过程中对隔震支座构造细节的认知要求相对较低。同时,建成后的BIM模型与实际工程在建筑信息的多个方面具有较好的一致性。相关研究可为建筑和桥梁隔震工程的BIM建模提供参考和借鉴。展开更多
BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple b...BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and inflammation.AIM To investigate the association between ferroptosis and pyroptosis and the upstream regulatory mechanisms.METHODS This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce ALF.Gasdermin D(GSDMD)^(-/-)mice were used as an experimental group.Histological changes in liver tissue were monitored by hematoxylin and eosin staining.ALT,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial kits.Ferroptosis-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain reaction.SIRT1,p53,and GSDMD were assessed by immunofluorescence analysis.RESULTS Serum AST and ALT levels were elevated in patients with ALF.SIRT1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver tissue.In the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was mitigated.In mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were detected.In vitro,knockdown of p53 and overexpression of GPX4 reduced AST and ALT levels,the cytostatic rate,and GSDMD expression,restoring SLC7A11 depletion.Moreover,SIRT1 agonist and overexpression of SIRT1 alleviated acute liver injury and decreased iron deposition compared with results in the model group,accompanied by reduced p53,GSDMD,and ACSL4,and increased SLC7A11 and GPX4.Inactivation of SIRT1 exacerbated ferroptotic and pyroptotic cell death and aggravated liver injury in LPS/D-GalNinduced in vitro and in vivo models.CONCLUSION SIRT1 activation attenuates LPS/D-GalN-induced ferroptosis and pyroptosis by inhibiting the p53/GPX4/GSDMD signaling pathway in ALF.展开更多
文摘BRIGHT blue sky, a fleet of diplomats＇ cars outside, flocks of journalists inside, a memorial site in the size of a small town： right next to the Lugou Bridge - better known in the West as the Marco Polo Bridge - is the location of the Museum of the War of Chinese People＇s Resistance Against Japanese Aggression.
文摘供热负荷预测是指导供热系统调控的重要手段。提高供热负荷预测精度十分重要,针对机器学习中输出目标的分解预测,提出了一种基于季节和趋势分解(seasonal and trend decomposition using loess,STL)的供热负荷预测方法,构建了适用于供热负荷预测的输出目标。首先利用STL算法将供热负荷时间序列数据分解为趋势分量、周期分量和残差分量,分别训练Informer、BiLSTM和XGB模型,将构建好的3个分量预测模型的输出叠加作为初步预测结果,分析误差序列,以BiLSTM预测误差提高模型精度,构建出STL-Informer-BiLSTM-XGB预测模型。将上述模型与常用预测模型进行对比,结果表明所构建的STL-Informer-BiLSTM-XGB模型的MAPE、MAE和MSE分别为0.871%、96.18和13202.2,预测效果最优,验证了所提出的方法具有较高的供热负荷预测精度。
文摘随着隔震技术的推广应用以及建筑业信息化水平的持续提升,在隔震工程中对隔震层建筑信息模型(building information modeling, BIM)建模的需求逐渐增长,然而针对性的研究工作相对较少。为此,围绕隔震支座BIM模型的高效建模方法和应用模块开展了研究。首先,综合隔震支座应用情况和力学特性,可将其分为橡胶隔震支座、滑移摩擦隔震支座和其他类型隔震支座,据此提出了隔震支座BIM快速建模模块基本架构;随后,基于Revit和Visual Studio平台开发了三类隔震支座BIM模型的快速建模功能,并实现了连接节点参数化建模和支座批量/手动布置的操作功能;最后,开展了某化工公司的库房隔震加固项目的隔震层BIM模型建模实践,结果表明:利用快速建模模块可将隔震层BIM建模操作从7个步骤降低至2个步骤,且使用过程中对隔震支座构造细节的认知要求相对较低。同时,建成后的BIM模型与实际工程在建筑信息的多个方面具有较好的一致性。相关研究可为建筑和桥梁隔震工程的BIM建模提供参考和借鉴。
基金Supported by National Natural Science Foundation of China,No.82060123Doctoral Start-up Fund of Affiliated Hospital of Guizhou Medical University,No.gysybsky-2021-28+1 种基金Fund Project of Guizhou Provincial Science and Technology Department,No.[2020]1Y299Guizhou Provincial Health Commission,No.gzwjk2019-1-082。
文摘BACKGROUND Acute liver failure(ALF)has a high mortality with widespread hepatocyte death involving ferroptosis and pyroptosis.The silent information regulator sirtuin 1(SIRT1)-mediated deacetylation affects multiple biological processes,including cellular senescence,apoptosis,sugar and lipid metabolism,oxidative stress,and inflammation.AIM To investigate the association between ferroptosis and pyroptosis and the upstream regulatory mechanisms.METHODS This study included 30 patients with ALF and 30 healthy individuals who underwent serum alanine aminotransferase(ALT)and aspartate aminotransferase(AST)testing.C57BL/6 mice were also intraperitoneally pretreated with SIRT1,p53,or glutathione peroxidase 4(GPX4)inducers and inhibitors and injected with lipopolysaccharide(LPS)/D-galactosamine(D-GalN)to induce ALF.Gasdermin D(GSDMD)^(-/-)mice were used as an experimental group.Histological changes in liver tissue were monitored by hematoxylin and eosin staining.ALT,AST,glutathione,reactive oxygen species,and iron levels were measured using commercial kits.Ferroptosis-and pyroptosis-related protein and mRNA expression was detected by western blot and quantitative real-time polymerase chain reaction.SIRT1,p53,and GSDMD were assessed by immunofluorescence analysis.RESULTS Serum AST and ALT levels were elevated in patients with ALF.SIRT1,solute carrier family 7a member 11(SLC7A11),and GPX4 protein expression was decreased and acetylated p5,p53,GSDMD,and acyl-CoA synthetase long-chain family member 4(ACSL4)protein levels were elevated in human ALF liver tissue.In the p53 and ferroptosis inhibitor-treated and GSDMD^(-/-)groups,serum interleukin(IL)-1β,tumour necrosis factor alpha,IL-6,IL-2 and C-C motif ligand 2 levels were decreased and hepatic impairment was mitigated.In mice with GSDMD knockout,p53 was reduced,GPX4 was increased,and ferroptotic events(depletion of SLC7A11,elevation of ACSL4,and iron accumulation)were detected.In vitro,knockdown of p53 and overexpression of GPX4 reduced AST and ALT levels,the cytostatic rate,and GSDMD expression,restoring SLC7A11 depletion.Moreover,SIRT1 agonist and overexpression of SIRT1 alleviated acute liver injury and decreased iron deposition compared with results in the model group,accompanied by reduced p53,GSDMD,and ACSL4,and increased SLC7A11 and GPX4.Inactivation of SIRT1 exacerbated ferroptotic and pyroptotic cell death and aggravated liver injury in LPS/D-GalNinduced in vitro and in vivo models.CONCLUSION SIRT1 activation attenuates LPS/D-GalN-induced ferroptosis and pyroptosis by inhibiting the p53/GPX4/GSDMD signaling pathway in ALF.
