Melatonin inhibits high glucose-induced cell proliferation and expressions of inflammatoryfactor via Toll-like receptor 4 signaling pathway inmouse mesangial cells

Objective To investigate effects of melatonin (MT)on high glucose-induced cell proliferation,Toll-like receptor4 ( TLR4) signaling pathway and expressions ofinflammatory factor in mouse mesangial cells ( SV40).Methods SV40 cells were divided into mannitol controlgroup ( 30 mmol /L mannitol ),normal control group(5 mmol /L glucose),control ( 5 mmol /L glucose) +1000 μmol /L MT group,high glucose group (25 mmol /L glucose),high glucose + 10,100,1000 μmol /L MTgroup and high glucose + TLR4 inhibitor ( TAK242 )group. (1) The cell viability was measured by CCK-8cytotoxicity kits,and cell proliferation was measured byEdU kits. The expression of TLR4 and the nuclear translocationof nuclear factor-κB ( NF-κB p65 ) were observedby immunofluorescence.