Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus...Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus, Bacillus or Streptococcus, are improved with different inoculum sizes. Purple non-sulphur bacterium, Afifella marina could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in Sargassum polycystum meal. Sargassum polycystum was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (A. marina were used with 20 g Sargassum polycystum powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30˚C ± 1˚C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with A. marina has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6<sup>th</sup> and 8<sup>th</sup> day. A. mariana with 30% (v/v) inoculum sizes on 6<sup>th</sup> of day has capability in improving the nutritional values of Sargassum polycystum seaweed meal during bioprocessing.展开更多
Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentrat...Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentration and toxic substances in PHWW undermine the efficiency of biomass production and nutrient recovery.This study aimed to investigate the effects of the microalgae strains,initial nutrients concentrations and inoculum sizes on biomass production and nutrient recovery using PHWW as the cultivation medium.Results indicated that both biomass production and nutrients recovery were successfully improved by using the screened microalgae strain at the desirable initial nutrient concentration with the suggested algae inoculum size.Chlorella vulgaris 1067 probably demonstrated the strongest tolerance ability among the five microalgae strains screened,and performed well in the diluted PHWW,of which initial TN concentration was approximately 500 mg/L.The desirable inoculum size was determined to be 0.103-0.135 g/L.The biomass daily productivity was increased by 15.67-fold(reached 0.13 g/(L·d)).With the above optimal conditions,high biomass production and nutrient recovery from the PHWW to produce microalgae biomass for bioenergy production were achieved.展开更多
以残留在筛网上的菌丝密集度为指标,确定了菌丝的最佳打碎时间为2 min。然后以杏鲍菇菌丝生长深度为指标,分别研究了打碎后的纯菌种稀释倍数和接种量对杏鲍菇菌丝生长的影响。结果表明:纯菌种最佳稀释倍数为500倍,最佳接种量为20 m L/...以残留在筛网上的菌丝密集度为指标,确定了菌丝的最佳打碎时间为2 min。然后以杏鲍菇菌丝生长深度为指标,分别研究了打碎后的纯菌种稀释倍数和接种量对杏鲍菇菌丝生长的影响。结果表明:纯菌种最佳稀释倍数为500倍,最佳接种量为20 m L/袋。此研究为筛选适用工厂化生产的液体菌种技术提供了理论依据。展开更多
文摘Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus, Bacillus or Streptococcus, are improved with different inoculum sizes. Purple non-sulphur bacterium, Afifella marina could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in Sargassum polycystum meal. Sargassum polycystum was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (A. marina were used with 20 g Sargassum polycystum powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30˚C ± 1˚C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with A. marina has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6<sup>th</sup> and 8<sup>th</sup> day. A. mariana with 30% (v/v) inoculum sizes on 6<sup>th</sup> of day has capability in improving the nutritional values of Sargassum polycystum seaweed meal during bioprocessing.
基金the National Natural Science Foundation of China(51576206,51308535)Beijing Science and Technology Program(Z161100001316009).
文摘Cultivating microalgae in post hydrothermal liquefaction wastewater(PHWW)offers many benefits,including nutrients recovery and reuse,wastewater purification and biomass production.However,the high nutrients concentration and toxic substances in PHWW undermine the efficiency of biomass production and nutrient recovery.This study aimed to investigate the effects of the microalgae strains,initial nutrients concentrations and inoculum sizes on biomass production and nutrient recovery using PHWW as the cultivation medium.Results indicated that both biomass production and nutrients recovery were successfully improved by using the screened microalgae strain at the desirable initial nutrient concentration with the suggested algae inoculum size.Chlorella vulgaris 1067 probably demonstrated the strongest tolerance ability among the five microalgae strains screened,and performed well in the diluted PHWW,of which initial TN concentration was approximately 500 mg/L.The desirable inoculum size was determined to be 0.103-0.135 g/L.The biomass daily productivity was increased by 15.67-fold(reached 0.13 g/(L·d)).With the above optimal conditions,high biomass production and nutrient recovery from the PHWW to produce microalgae biomass for bioenergy production were achieved.
