Effects of Microbial Inoculums on Soil Enzyme Activity and Microbial Diversity in a Reclaimed Mining Area

Through a pot experiment, effects of various microbial inoculums on soil microbial diversity and enzyme activity in a typical reclaimed mining area in Shanxi Province were discussed based on quantitative analysis of PLFA, soil urease, phosphatase and sucrase activity. The results showed that the application of microbial inoculums increased microorganism quantity in rhizosphere of rape by 2.3% -66.4%, and quantities of bac- teria, gram-positive bacteria, gram-negative bacteria, fungi and actinomycetes in the treatments with microbial inoculums were significantly higher than the contrast （P 〈0.05）, while there was no obvious change in protist quantity. In comparison with the contrast, the application of various mi- crobial inoculums also improved soil urease, sucrase and phosphatase activity by 4.2% - 61.4%, 18.0% - 32.5% and 64.2% - 199.0% respec- tively. It indicated that the application of microbial inoculums can improve soil microbial diversity and enzyme activity, so it is an effective way to sl^eed UP ecoloQical restoration of soil.

Effects of Seed Dressing with Microbial Inoculum on Nutrient Composition and Biological Yield of Silage Corn

[Objectives]This study was conducted to understand the effects of microbial inocula on the biological yield and nutritional components of corn.[Methods]Silage corn varieties suitable for planting in Hebei Province were selected,and set with an experimental group and a control check group each.Meanwhile,at the late stage of milk ripening of silage corn growth cycle,the nutritional components and indexes of silage corn were determined and analyzed by a near-infrared analyzer and the NIRS technique.Meanwhile,the biological yield of silage corn was determined at the maturation stage of its growth period,aiming at comprehensively evaluating the regulation effects of the microbial inoculum tested on silage corn from the aspects of basic nutritional components,mineral element contents,energy and related indexes of corn,combined with agronomic characters and biological yield.[Results]The microbial inoculum improved the biological yield and nutritional indexes of silage corn,and had a positive regulation effect on the growth of silage corn.[Conclusions]The results of this study provide a theoretical basis for popularizing the planting methods and cultivation methods of silage corn with high yield and high quality.

Isolation and Molecular Characterization of Elite Indigenous Rhizobia Nodulating Phaseolus bean (Phaseolus vulgaris L.)

Nowadays application of biological nitrogen fixation (BNF) through rhizobia inoculums is highly promoted as a solution to solve the problem of poor soil fertility in areas where legumes are cultivated. This is due to the fact that, rhizobia enhance nitrogen fixation, induce disease resistance, reduce heavy metal in the soil, facilitate bioavailabity of iron in soil and is environmental friendly. To get rhizobia strains which are suitable for inoculants production, isolation and molecular characterization of elite rhizobia are highly needed. Molecular characterization acts as a spark plug for discovery of many microbes including Rhizobia. Multi Locus Sequence Analysis (MLSA), 16S rRNA gene sequence analysis, DNA-DNA hybridization and SDS-PAGE analysis of the whole-cell proteins are the molecular techniques mostly used in characterizing rhizobia. But before deciding to use or not to use rhizobia inoculants in certain areas, knowing the population size of indigenous rhizobia found in that area is very important, because this is a major factor which determines inoculums responses as well gives clues on which areas need or do not need inoculation. The Most Probable Number (MPN) method is mostly used in enumerating rhizobia population of the soil. Given that, in most of the developing countries, including Tanzania, Biological Nitrogen Fixation (BNF) technology is not fully flourished;more efforts in isolation, molecular characterization of elite rhizobia and estimation of indigenous rhizobia population in various areas are required.

History and perspectives of induction technology for agarwood production from cultivated Aquilaria in Asia: a review

Agarwood, the resinous product of Aquilaria spp.(Thymelaeaceae), is highly valued for medicinal and fragrant purposes. Unsustainable forest harvesting contributes to the declining population in the wild, threatening their existence. As a protection effort, cultivation occurred in range countries, mainly in Asia, effectively establishing mass plantations. The success of domesticating Aquilaria relies heavily on technological advancement in agarwood induction, without which the entire thriving industry will collapse. In this paper, we describe efforts since 1929 and current progress and variation in induction as practiced in Asia. The methods include traditional practices and artificial induction via inoculum and chemical inducer, experimented and patented or otherwise. Artificial induction methods are being developed to intensify agarwood production in terms of yield and quality. While traditional methods are commonly applied by rural planters, artificial methods with the use of specific agents/chemicals are seen as more appealing for inducing agarwood, and have a higher demand from entrepreneurs and plantation owners.Several issues related to induction technology faced by stakeholders in agarwood cultivation are presented, such as safety levels, cost, yields, and quality. We conclude by highlighting remaining challenges in induction methods and their associated technologies.

Analyses of the Temporal Development and Yield Losses due to Sheath Blight of Rice (Rhizoctonia solani AG1.1a)

Sheath blight of rice, caused by Rhizoctonia solani AG 1. 1a, has become the most important disease and caused serious yield losses in some major rice-growing regions in China in recent years. In the present study, field plot experiment was conducted to examine the relationships between disease intensity and inoculum density （ID）, the seasonal disease epidemic dynamics, and yield reductions due to disease damages. Results from the experiment demonstrated that the areas under progress curves of disease severity and those of percent rice tillers diseased were positively and closely related to the relative initial ID of the pathogen. The inoculum density-disease （IDD） relationships were simulated and the impractical linear models were obtained. Both logistic and Gompertz functions could be used to simulate the disease progress dynamics in time, but the progress curves of the disease severity were modeled better by the Gompertz than by logistic function. However, the Richards function was found to be the best in simulating the disease progress curves when a most appropriate value was chosen for the shape parameter m by using the computer software Epitimulator. Sheath blight infection decreased rice yield very significantly and a yield reduction of 40% was recorded in rice crop with the highest inoculum density. Rice yield was linearly and negatively correlated with the disease severity and the percent tillers affected. The simulated models for all these relationships were computed through executing Epitimulator software and were presented in this paper.

Transformation of organic matters in fresh leachate during anaerobic degradation under long hydraulic retention time

This study investigated the biodegradability of fresh leachate from a young municipal solid waste landfill at the inoculum to substrate ratios （V/V） of 10/100, 25/100, 50/100 and 100/100, as well as the transformation of organic matters in leachate under prolonged hydraulic retention time （HRT） of 50 d. Fresh leachate showed a good biodegradability, with chemical oxygen demand （COD） removal efficiency as high as 87%-92% and cumulative methane yield close to theoretical value （0.35 L CH4/g CODdeg）. Methane production and COD depletion presented biphasic characteristics associated with the successive utilization of two major intermediates, acetate and propionate, The biphasic degradation of fresh leachate was resulted from the different hydrolysis rates of diverse substrates and the changes in microorganism community structure. After 50 d, the effluents were dominated by high-MW organic compounds （MW 〉 10 kDa） at each inoculum ratio, which might be refractory compounds released from cell lyses.

Minor modifications in obtainable Arabidopsis floral dip method enhances transformation efficiency and production of homozygous transgenic lines harboring a single copy of transgene

Many researchers have developed various methods for in-planta or floral dip transformation of Arabidopsis thaliana, one of the simple protocol and widely used to produce transgenic Arabidopsis. As the efficiency and ease of getting a transformant is very much time consuming effort and less number of the transformants people get, we have developed a little modified transformation protocol to avoid the disparities. Four types of inoculums (inoculum1, inoculum2, inoculum3 and inoculum4) were used to check the transformation efficiency out of which Inoculum3 showed the highest rate of transformation among the four types. 0.07% Twin-20 also acts in same manner as silwet L-77 to increase the rate of transformation efficiency and glucose instead of sucrose can be used in inoculum to transform Arabidopsis. After vacuum infiltration keeping the Agrobacterium infected plants for 7-8 hrs horizontally in low light at 280C temperature condition, considered best to get an increased number of transformed seeds. Modified protocol produced ~12-14% increase in transformants. Selection pots (kanamycin supplemented soil filled pots) in place of selection plates (Kanamycin supplemented Murashige and Skoog agar plates) proved beneficial as no MS medium and no aseptic condition is required for selection of transformed plants. This increase in transformation efficiency consequently increased the percentage of homozygous and single copied stable transgenic lines.

The effect of particle size and amount of inoculum on fungal treatment of wheat straw and wood chips

Background： The aim of this study was to optimize the fungal treatment of lignocellulosic biomass by stimulating the colonization. Wheat straw and wood chips were treated with Ceriporiopsis subvermispora and Lentinula edodes with various amounts of colonized millet grains（0.5, 1.5 or 3.0 % per g of wet weight of substrate） added to the substrates. Also, wheat straw and wood chips were chopped to either 0.5 or 2 cm.Effectiveness of the fungal treatment after 0, 2, 4, 6, or 8 wk of incubation was determined by changes in chemical composition, in vitro gas production（IVGP） as a measure for rumen degradability, and ergosterol content as a measure of fungal biomass.Results： Incomplete colonization was observed for C. subvermispora treated wheat straw and L. edodes treated wood chips. The different particle sizes and amounts of inoculum tested, had no significant effects on the chemical composition and the IVGP of C. subvermispora treated wood chips. Particle size did influence L.edodes treatment of wheat straw. The L. edodes treatment of 2 cm wheat straw resulted in a more selective delignification and a higher IVGP than the smaller particles. Addition of 1.5 % or 3 % L. edodes inoculum to wheat straw resulted in more selective delignification and a higher IVGP than addition of 0.5 % inoculum.Conclusion： Particle size and amount of inoculum did not have an effect on C. subvermispora treatment of wood chips. At least 1.5 % L. edodes colonized millet grains should be added to 2 cm wheat straw to result in an increased IVGP and acid detergent lignin（ADL） degradation.

Evaluation of host resistance and fungicide application as tools for the management of root rot of field pea caused by Aphanomyces euteiches

Aphanomyces root rot(ARR) of field pea(Pisum sativum), caused by Aphanomyces euteiches, can cause severe root damage, wilting, and large yield losses under wet soil conditions. To identify ways to manage this disease, the effect of A. euteiches inoculum density on field pea was studied under greenhouse and field conditions in 2015 and 2016. Increases in inoculum density reduced seedling emergence, root nodulation, and plant vigor, and resulted in increased root rot severity in both field and greenhouse tests. Seed treatments with the fungicides Apron Advance(thiabendazole + fludioxonil + metalaxyl) + Vibrance(difenoconazole + metalaxylM + sedaxane), INTEGO Solo(ethaboxam), BAS 516F(boscalid + pyraclostrobin), BAS 720F(metalaxyl + pyraclostrobin + fluxapyroxad), and BAS 516F + BAS 720F(3:1) were evaluated for their efficacy against ARR. All seed treatments except Apron Advance + Vibrance reduced root rot severity under controlled conditions. BAS 516F, BAS 720F and INTEGO Solo improved plant vigor and all treatments reduced seedling blight to varying degrees under greenhouse conditions, but not in the field. A collection of 22 pea genotypes was evaluated for resistance to root rot in field plot experiments. Line 00–2067 showed the least severe root rot symptoms,whereas ‘Spring D' showed the lowest reduction in yield. The results suggest that there may be an opportunity to combine partial host resistance and fungicidal seed treatments to adequately manage ARR of field pea.

Source of mycorrhizal inoculum influences growth of Faidherbia albida seedlings

Poor land use management and practice inhibit the growth and establishment of tree seedlings in dryland areas.We assessed arbuscular mycorrhizal fungi(AM)status of Faidherbia albida(Del.)A.Chev.trees grown on different land uses.We quantified the growth and nutrient uptake of F.albida seedlings inoculated with AM from different sources.These efforts were based on soil and fine root samples from the rhizosphere soils of F.albida trees.AM root colonization was determined using the gridline intersect method.Spores were extracted by the wet sieving and decanting method and identified to genus level.The seedling experiment had a completely randomized onefactorial design with four treatments and five replications.Faidherbida albida seedlings were grown in a greenhouse.All in situ F.albida trees were colonized by AM fungi.AM root colonization of F.albida trees was significantly higher(P<0.0086)in area exclosures than on lands used for grazing or cultivation.Spore abundance was significantly higher(P<0.0014)in area exclosures followed by cultivated land and grazing land.Glomus was the dominant genus in all land-uses.AM-inoculated F.albida seedlings grew better(P<0.05)than non-inoculated controls.Seedlings inoculated with AM from area exclosure had significantly(P<0.05)higher growth and nutrient uptake than those inoculated with AM from grazing and cultivated land.This emphasizes the importance of the native soil AM potential for better establishment of seedlings to achieve optimum plant growth improvement and assist in rehabilitation of degraded arid lands.

Influence of Traditional Inoculum and Fermentation Time on the Organoleptic Quality of "Attiéké”

To improve the process of “Attiéké” production by the standardization of the traditional inoculums, the effects of inoculums amount (6%, 8%, 10% and 12%) and fermentation time (6, 12 and 18 h) were studied and analyzed for their microbiological and sensory qualities using standard analytical procedures. The microbial analysis showed that Lactobacillus species (9.14 Log cfu/g) as the most important popular microorganism in the paste, followed by enterococci (7.64 Log ufc/g) species, yeasts and moulds (7.30 Log cfu/g) respectively. Sensory analysis revealed significant effects (p < 0.05), of inoculums quantity and fermentation time on the “Attiéké” quality. “Attiéké” obtained with 10% inoculums and after 12 hours of fermentation was identified as the optical inoculums amount and time for “Attiéké” production. The importance of traditional starter inoculums in “Attiéké” processing could be necessary to standardization at small and industrial scale the process of production.

Integrated Effects of Rhizobial Inoculum and Inorganic Fertilizers on Wheat Yield and Yield Components

An experiment was conducted in pots under natural condition with two factor factorial completely randomized design (CRD) (CRD) to investigate the integrated effect of rhizobia inoculums and inorganic fertilizers on growth and yield of wheat crop at The University of Agriculture Peshawar, during 2012-2013. The experiment was comprised of four inoculums (no, lentil, peas and chickpeas) and two NPK levels (recommended 120:90:60 kg·ha-1 & 20% less of recommended). It was observed that inoculation of wheat by rhizobia significantly increased tillers per plant by a maximum of 42%, plant height by 13%, grain per spike by 16%, 100 grain weight by 10%, biological and grain yield by 10% over un-inoculated (control) treatment. Among inoculums, peas inoculum was found to be the most efficient for all traits except plant height where chickpea inoculum performed better. Similarly recommended NPK significantly increased tillers per plant by 33%, plant height by 19%, grain per spike by 9%, 100 grain weight by 10%, biological yield by 8% and grain yield by 10% compared with 20% less of recommended NPK. Interactive effect of inoculum x NPK was significant for tillers per plant, grain per spike, grain yield and non-significant for plant height, 100 grain weight and biological yield. However, it was evident from the results that inoculation improved all traits both under recommended and 20% less of recommended NPK. The persistent good performance of peas and lentil rhizobial inoculation in wheat growth exhibited that this could be used as a plant growth promoting rhizobacteria for wheat and other cereal crops in prevailing soil and climatic conditions.

Effect of Lactate and Starter Inoculum on Biogas Production from Fresh Maize and Maize Silage

Lactate is a key intermediate during anaerobic digestion of carbohydrates;however, it fails to receive significant consideration in biogas plants. We examined the influence of lactic acid on biogas production. Two commonly used feeds, fresh maize and maize silage, were selected as substrates due to their difference in lactic acid contents. Additionally, inocula from an agriculture-based biogas plant, a waste water treatment plant and a standardised laboratory reactor were selected to investigate the impact of starter culture on the process. Experiments demonstrated increased total biogas yield of up to 45% in the lactate-rich maize silage over the lactate-devoid fresh maize, but only in cases where the starting inocula had been previously exposed to lactic acid. Our findings suggest lactic acid is a significant intermediate in biogas production and merits consideration. Additionally, the ability of the starter inoculum to utilize lactic acid is an important factor in process optimization and enhanced biogas production.

Improving Compost Process Efficiency by Leachates Inoculation and Shredding of the Organic Fraction of Municipal Solid Waste at Bordo Poniente Composting Plant,Mexico City

Management of MSW(Municipal Solid Waste)is a major downside in most of the biggest cities.The composting of the organic fraction of MSW is one of the oldest and simplest ways of organic waste stabilization.It is a self-heating biological conversion that generates appropriate finished merchandise such as soil conditioner or fertilizers.Mexico City generates about 12,500 ton/day of MSW,44%of it is food scraps and yard trimmings which are the OFMSW(Organic Fraction of Municipal Solid Waste),2,500 ton/day of it is composted at the BPCP(Bordo Poniente Composting Plant)yielding 500 ton/day of compost.The purpose of this study was to evaluate three treatments to accelerate the composting process,so eventually the city could increase the amount of the OFMSW at BPCP.We compared three different treatments;one of them showed a significant reduction in time of the composting process(p≤0.05)i.e.,it took less time to reach the thermophilic stage,maturity and stabilization phases.Maturity was achieved at 35 days and 60 days to stabilize.We conclude that shredding the feedstock plus leachate inoculum addition at the beginning of the process,reduces the composting time in about 61%with respect to the time it takes at the BPCP,where lasted 90 days.

Hygienic Quality and Nutritional Value of Attiékéfrom Local and Imported Cassava Dough Produced with Different Traditional Starters in Burkina Faso

Attiéké is an essentially flavour starchy food produced from fermented cassava root. The product is widely consumed in Burkina Faso. The objective of the present study was to investigate the biochemical and the microbiological characteristics of attiéké from Burkina Faso. The samples (36) were collected from six (6) producers. Cassava dough which is used for attiéké production contains from 70.67% ± 0.25% to 86.02% ± 0.2% as starch. The undesirable organic elements rate is about 1.28% ± 0.14% to 26.46% ± 0.53%. The inorganic impurities rate is about 0 to 7 ± 0.1 mg/100g. Cassava dough acidity varies from 1.8 ± 0.2 to 12.4 ± 0.1;and its pH from 3.48 ± 0.01 to 4.20 ± 0.01. Lactic bacteria are the main microorganisms involved in cassava dough fermentation (5.17 to 9.30 log cfu/g). Yeasts and molds number is low (<6.53 log cfu/g). Attiéké moister is about 50.6% ± 0.00% to 55.12% ± 0.7% and its protein contents from 0.77 to 1.74 ± 0.13 g/100g. The content in lipid of attiéké shows an important variation and is about 0.15 to 3.28 ± 0.32 g/100g. Carbohydrates content varies from 36.6 ± 0.04 to 47.01 ± 0.1 g/100g and its ashes content is from 140 to 780 ± 20 mg/100g. Attiéké acidity and pH are less weak than cassava dough. They vary respectively from 0.92% ± 0.05% to 4.08% ± 0.57% and from 3.7 to 4.4 ± 0.01. As a main energizer food, attiéké energizing value is from 161.95 to 215.26 Kcal/100g. All the attiéké analyzed was exempt of aflatoxin (B1, B2, G1, G2) and ochratoxin A. The local attiéké has higher acidity, fermented bacteria load, protein and minerals salt than the imported one. The process mastery is the mainly factor that determines nutritional and sanitary quality of attiéké.

Effects of Inoculum Size of Afifella marina in the Bioprocessing of Sargassum spp. Meal

Brown seaweed, Sargassum spp. is also used as an alternative feed ingredient for aquaculture in the form of seaweed meal. Nutritional values seaweed meal with bioprocess technology using microbes such as Lactobacillus, Bacillus or Streptococcus, are improved with different inoculum sizes. Purple non-sulphur bacterium, Afifella marina could be one of the alternative and potential candidates. This study aims to determine the optimum inoculum level and period for the improvement of nutritional values in Sargassum polycystum meal. Sargassum polycystum was collected from the Sepanggar Bay. Dried seaweed was grinded into fine particles (A. marina were used with 20 g Sargassum polycystum powder in one liter bottle with autoclaved 112 synthetic media. Bottles were incubated at 2500 lux light intensity at 30˚C ± 1˚C for 8 days. Every two days destructive samples were taken for the determination of nutritional values of bioprocessed seaweed meal. The measured analytical parameters were crude protein (%), crude ash (%), crude fiber (%) and crude lipids (%). Nutritional value of processed seaweed meal powder with A. marina has improved with the increase of inoculum size. The crude protein percentage of 14.70% + 0.40% was significantly high (p 0.05) were observed among the values of crude protein, ash, lipids and fiber within 6th and 8th day. A. mariana with 30% (v/v) inoculum sizes on 6th of day has capability in improving the nutritional values of Sargassum polycystum seaweed meal during bioprocessing.

Effect of Different Concentrations of the Suspensions of B.amyloliquefaciens and T.harzianum on the Development of C.acutatum

The effect of nine isolates of Bacillus amyloliquefaciensand one strain of Trichoderma harzianum, TR, on mycelial growth and germination of Colletotrichum acutatum were studied. The nine isolates were identified as Bacillus amyloliquefaciens. The efficacy of isolates was tested, at different concentrations. Results showed that one Bacillus isolates (Bc2) and TR were more effective at the lower concentration tested (3 × 105 CFU/ml and 105 conidia/ml).

Evidence for transmission of aphid-pathogenic fungi by migratory flight of Myzus persicae alates

The alates of the green peach aphid, Myzus persicae, were daily trapped from the air from late October through early January and carried to laboratory for determination of fungal infection by individually rearing them for 7 d on detached cabbage leaves in Petri dishes. Among 760 alates trapped, 266 (35%) were found carrying various fungal pathogens, 87.3% of them died due to mycosis during the first 3-day period of rearing and the rest died in the following two days. Most of the deaths of the alates were attributed to entomophthoralean fungi, taking 94.4%, and the rest were the hyphomycetous fungus Beauveria bassiana. Among the Entomophthorales-killed alates, P. neoaphidis took a proportion of 66.1%, Z. anhuiensis 22.6%, E. planchoniana 9.7%, and N. fresenii 1.6%, respectively. Two alates were found suffering from cross infection of two fungal species, i.e. P. neoaphidis with Z. anhuiensis and N. fresenii, respectively. The results represent the first report on transmission of aphid-pathogenic fungi

Ruminal fermentation and microbial community differently influenced by four typical subtropical forages in vitro

The present study evaluated the effects of 4 typical subtropical forages on ruminal microbial community composition to formulate a better diet for buffalo.Corn straw silage,elephant grass,cassava residues and sugarcane tail silage were used as substrates for in vitro fermentation.Eight replicates were set up for every substrate,and fermentation was carried out in a 100-m L glass syringe,using buffalo rumen inoculum.Every replicate was anaerobically dispensed with 10 m L of rumen inoculum,20 m L of Mc Dougall's buffer and 200 mg of dried substrate,and placed in a water bath at 39C.Gas production was recorded at 0,2,6,12,24,36,48 and 72 h of incubation.After 24 h,fermentation was ceased for 4 replicates and samples were collected.Volatile fatty acids(VFA) concentrations were measured using gas chromatography.Microbial populations were quantified using quantitative real-time PCR(q RT-PCR),and microbial community was analyzed using high throughput sequencing technology.The results showed,cassava residues as substrate had the highest gas production,acetate,propionate and total VFA concentrations(P < 0.05),and corn straw silage had the lowest acetate:propionate ratio(P < 0.05).The lowest numbers of fungi,Ruminococcus albus and Fibrobacter succinogenes,and the highest number of protozoa were observed with cassava residues(P < 0.05).The least abundances of bacterial phyla Firmicutes,Bacteroidetes and genus Prevotella,and substantially higher abundance of phylum proteobacteria(56%) and genus Succinivibrio(52%) were observed with cassava residues.The most abundances of Methanobrevibacter gottschalkii and Entodinium were observed with cassava residues.Spearman's correlations analysis showed,Succinivibrio had strong positive correlations with propionate,butyrate,Metadinium and M.gottschalkii,indicating fermentation products were related to microbial community.In conclusion,incubation with cassava residues resulted in lower number of fiber degrading microbes but higher protozoal population because of its low fiber contents.The microbial community was highly altered by in vitro incubation with cassava residues,whereas remained similar for the other 3 high fiber containing substrates.

Optimization and modeling of glyphosate biodegradation by a novel Comamonas odontotermitis P2 through response surface methodology

Glyphosate is an important organophosphonate herbicide used to eliminate grasses and herbaceous plants in many vegetation management situations.Its extensive use is causing environmental pollution,and consequently,there is a need to remove it from the environment using an eco-friendly and cost-effective method.As a step to address this problem,a novel bacterial strain Comamonas odontotermitis F2,capable to utilize glyphosate as a carbon(C)and/or phosphorus(P)source,was isolated from a glyphostate-contaminated field soil in Australia and characterized.Response surface methodology(RSM)employing a 2^3 full factorial central composite design was used to optimize glyphosate degradation by C.odontotermitis P2 under various culture conditions.The strain C.odontotermitis P2 was proficient in degrading 1.5 g L^-1 glyphosate completely within 104 h.The optimal conditions for the degradation of glyphosate were found to be pH 7.4,29.9℃,and an inoculum density of 0.54 g L^-1,resulting in a maximum degradation of 90%.Sequencing of glyphosate oxidoreductase(GOX)and C-P lyase(phnJ)genes from C.odontotermitis P2 revealed 999c and 93%identities to already reported bacterial GOX and phnJ genes,respectively.The presence of these two genes in C.odontotermitis indicates its potential to degrade glyphosate through GOX and C-P lyase metabolic pathways.This study demonstrates the potential of C.odontotermitis P2 for efficient degradation of glyphosate,which can be exploited for remediation of glyphosate.