Effects of Long-Term Application of Inorganic Fertilizers on Biochemical Properties of a Rice-Planting Red Soil

A long-term experiment was set up in Yingtan of Jiangxi Province to investigate the effects of long-term application of inorganic fertilizers on the biochemical properties of a rice-planting soil derived from Quaternary red earth. Noncultivated soils are extremely eroded and characterized by a low pH and deficiencies in available nutrients, in particular P and N. After 13 years of inorganic fertilization in cultivation for double-cropped rice, the biochemical properties of the soil were changed. The nitrification potential and urease activity were higher in the treatments with N application than those without N application, Acid phosphatase activity and dehydrogenase activity were also higher in the treatments with P application than in those without P application. The dehydrogenase activity correlated well with the concentrations of both total P and hydrolysable N and with rice crop yield, suggesting that dehydrogenase activity might be a suitable indicator for improvement in soil fertility.

Responses of Nematode Communities to Inorganic Fertilizer Disturbance in a Farmland Ecosystem

Nematode communities were monitored in a field study at depths of 0～10 cmand 10～20 cm throughout a corn (Zea mays L.) growing season in the black soil region of NortheastChina. Plots were cropped corn with (IF) and without (NF) nitrogen fertilization. Fifteen familiesand 16 genera were observed in the nematode suspensions. Cephalobidae, Rhabditidae, Aphelenchus,Filenchus, and Helicotylenchus were found to be dominant genera/families in both plots at bothdepths. Significant differences were found between treatments and corn growing stages in the totalnumbers of nematodes during the study period. The total numbers of nematodes at the 0～10 cm depthwere higher in the inorganic fertilizer plot than in the control plot (no fertilizer applications)across jointing, filling, and mature stages. Bacterivores were the most abundant trophic groupsunder two treatments and at both depths, and the relative abundance averaged 42.8%.Omuivores-predators at both depths were lower in the inorganic fertilizer plot than in the controlplot during the study period, excluding corn jointing stage. Maturity index (MI) and plant parasiteindex (PPI) were used to detect the differences between the two treatments, MI and PPI wereeffective in distinguishing differences in nematode communities during corn growing season. The roleof nematode communities as bioindicators of ecosystem disturbance due to fertilization is noted.

Combined Application of Organic and Inorganic Fertilizers on Black Soil Fertility and Maize Yield

By two years （2007-2008） located fertilizer experiment, the effect of long-term combined application of organic and inorganic fertilizers on black soil fertility and crop yield was investigated in Shuangcheng City, Heilongjiang Province. The results showed that the combined application of organic and inorganic fertilizers could increase the organinc matter, alkaline nitrogen, available phosphorus and available potassium. At the same time, the increasing application of organic fertilizer could reduce the soil bulk density and improve the field moisture capacity. Field moisture capacity and organic matter of the combined application of high quantities of organic manure and inorganic fertilizers AtB5 treatment increased the fhstest, organic matter increased by 3.33 g. kg and field moisture capacity increased by 11.25% than the beginning of the experiment. Under the same fertilization, the combined application of organic and inorganic fertilizers＇ increasing production range was higher than the single chemical fertilizers＇ which was from 0.8% to 9.4%. The results showed that the combined application of organic and inorganic fertilizers could increase the nutrient contents of soil and also was the highest productivity contribution to black soil fertility. It was the best fertilization structure of increasing productivity level and improving the soil fertility.

Effect of Integrated Use of Inorganic Fertilizer and Organic Manures on Bacterial Wilt Incidence (BWI) and Tuber Yield in Potato Production Systems on Hill Slopes of Central Kenya

Bacterial wilt (BW) caused by Ralstonia solanacearum is one of the most damaging diseases of potato (Solanum tuberosum L.) in Kenya and worldwide. In Kenya, potato tuber yield losses due to BW infection are estimated at 50 ~ 100%. Low soil fertility is also one of the most important constraints limiting potato production in central Kenya highlands. Farmers tackle this problem through use of inorganic fertilisers and organic manures, both of which amend the soil environment to influence bacterial wilt development. Undecomposed organic manures can also introduce the pathogen into a clean field. Between short rains 1999 and 2000, 10 on-farm extension-researcher- farmer-designed and farmer-managed trials were done at Kianjuki catchment in Embu District. The objective was to use farmers?participatory research approach and select the most suitable organic and inorganic fertiliser combination(s) with lowest BWI and acceptable usable tuber yields, and also to demonstrate use of some components of integrated disease management methods in reduction of disease incidence and spread. Seven treatments were proposed, presented to the farmers for discussion and the most relevant four were selected for evaluation. A newly released potato variety 慉sante?was planted during the short rains 1999 and long rains 2000. BWI didn抰 result in significant differences between treatments but the tuber yields were significantly different in short rains of 1999 and 2000. During short rains 2000, both BWI and tuber yields and unusable tubers differed significantly between treatments. The results confirmed that well- decomposed manures or manures from pathogen-free areas can be used in combination with inorganic fertilisers to improve soil fertility and potato tuber yields in smallholder farms without influencing BWI. Use of certified seed tubers in pathogen free field and following recommended field sanitation measures resulted in apparently bacterial wilt free crop. Considering the high cost of inorganic fertiliser and its negative effects on the environment, reduced usage at half the recommended rates combined with half rates of FYM is feasible option friendly to the farmer, soil and environment. The interviewed farmers also ranked the option as the most appropriate combination for soil fertility improvement for potato production in smallholder farms.

Effects of inorganic fertilizers on biological nitrogen fixation and seedling growth of some agroforestry trees in Bangladesh

The effects of different inorganic fertilizers （Urea and Triple Super Phosphate （TSP）） on seedling growth and nodulation capabilities of four agroforestry tree species （Albizia chinensis, A. saman, Acacia nilotica and Sesbania sesban） were compared. The nodulation of these seedlings were treated with different fertilizer treatments （at the rate of urea 40 kg·hm^-2, urea 80 kg·hm^-2 TSP 40 kg·hm^-2, TSP 80 kg·hm^-2, （urea＋TSP） 40 kghm 2 and （urea＋TSP） 80 kg·hm^-2） after one month of seed germination. The results revealed that the seedling growth was enhanced significantly with moderate fertilizer treatment. In some cases, the higher levels of fertilizers reduced the seedling growth. The study also revealed that the nodulation in nodule number and size was significantly inhibited by the application of N fertilizer （Urea）, while it was increased significantly with the application of P fertilizer （TSP）. This study improved our understanding and provided insights that would be useful to the farmers in their efforts to amend the soil with inorganic fertilizers in order to enhance plant growth and biological nitrogen fixation.

Vermicompost as an Alternative to Inorganic Fertilizer to Improve Okra Productivity in Côte d’Ivoire

Okra is one of the most popular vegetables in Côte d’Ivoire which is produced by heavy use of inorganic fertilizers. Vermicompost can be an alternative to inorganic fertilizers. This field study investigated the effect of vermicompost on growth and productivity of okra as compared to inorganic fertilizers. The respective treatments were arranged in a complete randomized block design, each at three replications, during three-season cycles on a ferralitic soil. Results showed that the highest rate of germination was obtained with the vermicompost. The tallest plants of Abelmoschus esculentus (1.88 m) and Abelmoschus caillei (1.78 m) were observed with inorganic fertilizer. The number of leaves per plant registered when using vermicompost was 34.5 and 30.74 with Abelmoschus esculentus and Abelmoschus cailli, respectively. With inorganic fertilizer, the number of leaves per plant was 34.21 (Abelmoschus esculentus) and 32.32 (Abelmoschus cailli). Plants took about 60 days to flower in the control plots and about 46 days in the plots fertilized with the vermicompost and the inorganic fertilizer. The highest pod yields of Abelmoschus esculentus and Abelmoschus cailli were 8.7 t⋅ha−1 and 10.58 t⋅ha−1 with vermicompost and 8.85 t⋅ha−1 and 10.7 t⋅ha−1 with inorganic fertilizer, respectively. Vermicompost could be recommended as an alternative to inorganic fertilizer to produce okra on ferralitic soil in Côte d’Ivoire.

Effects of Green Manure and Inorganic Fertilizers on the Growth, Yield and Yield Components of Soybean (Glycine max (L.) Merr.) in the Mount Cameroon Region

This study evaluates the effects of green manure, inorganic fertilizers and their combinations on the growth and yield of soybeans in the Mount Cameroon Region. The experiment involved 10 treatments which include, 5 Mg/ha green manure, 180 Kg P/ha triple superphosphate fertilizer (P), 180 Kg/ha NPK (20:10:10), 2 Mg/ha green manure + 90 kg P/ha, 3 Mg/ha green manure + 90 kg P/ha, 5 Mg/ha green manure + 90 Kg P, 2 Mg/ha green manure + 90 kg NPK/ha, 3 Mg/ha green manure + 90 kg NPK/ha, 5 Mg/ha green manure + 90 kg NPK and a control. Plant growth characters were assessed every 14 days for a period of 70 days for the late (August) and early (March) planting seasons. The results indicated that plants supplied with 5 Mg/ha green manure had the highest plant heights of 60.7 cm, and this was significantly different from the control which had the least height (52.76 cm). Stem collar diameter was significantly (p 0.001) increased with treatments in both seasons. Plants supplied with 5 Mg/ha green manure recorded the highest stover yield (10.59 Mg/ha), and this was significantly different from the control which had the least (6.64 Mg/ha). Plants supplied with 5 Mg/ha green manure had the highest days to flowering (38 days), while plants supplied with 5 Mg/ha green manure + 90 Kg NPK had the least (33 days) which was significantly different (p 0.01). The highest number of pods per plant was observed in plants supplied with 5 Mg/ha green manure (57 pods per plants), which was significantly different from the control (39 pods per plant). Plants supplied with 5 Mg/ha green manure + 90 Kg NPK recorded the highest grain yield (4.97 Mg ha-1), while the lowest grain yield was observed in the control (2.22 Mg ha-1). Results from this study can be used by soybeans farmers in the Mount Cameroon region to enhance productivity of this crop and thereby improving their living standards and food security in Cameroon.

Growth and Yield of Rice(Oryza sativa L.)Applied by Inorganic and Organic Fertilizer in Indonesian Tidal Soil

The aims of this study were to determine the dose of inorganic fertilizer of NPK combined with organic fertilizer from CM(Cow Manure)to grow paddy Inpara 8 variety planted in a tidal soil.This experiment was done in a green house and conducted from July to December 2020.This pot experiment used factorial completely randomized design with two factors and three replications.The first factor was NPK which consisted of two kinds of NPK fertilizers which were urea 200 kg/ha,SP-36100 kg/ha,KCl 100 kg/ha from recommended fertilization and urea 37 kg/ha,SP-3656 kg/ha,KCl 183 kg/ha from calculation of the specific location soil analysis(in situ data).The second factor was dosage of CM at 5,7.5 and 10 tons/ha respectively.The results showed that NPK fertilization from specific location combined with CM at 10 tons/ha was the best treatment for rice production in an infertile tidal swampland producing 2.20 tons/ha.

Mitigation of N_(2)O emissions in water-saving paddy fields:Evaluating organic fertilizer substitution and microbial mechanisms

Water-saving irrigation strategies can successfully alleviate methane emissions from rice fields,but significantly stimulate nitrous oxide(N_(2)O)emissions because of variations in soil oxygen level and redox potential.However,the relationship linking soil N_(2)O emissions to nitrogen functional genes during various fertilization treatments in water-saving paddy fields has rarely been investigated.Furthermore,the mitigation potential of organic fertilizer substitution on N_(2)O emissions and the microbial mechanism in rice fields must be further elucidated.Our study examined how soil N_(2)O emissions were affected by related functional microorganisms(ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),nirS,nirK and nosZ)to various fertilization treatments in a rice field in southeast China over two years.In this study,three fertilization regimes were applied to rice cultivation:a no nitrogen(N)(Control),an inorganic N(Ni),and an inorganic N with partial N substitution with organic manure(N_(i)+N_(o)).Over two rice-growing seasons,cumulative N_(2)O emissions averaged 0.47,4.62 and 4.08 kg ha^(−1)for the Control,Ni and N_(i)+N_(o)treatments,respectively.In comparison to the Ni treatment,the N_(i)+N_(o)fertilization regime considerably reduced soil N_(2)O emissions by 11.6%while maintaining rice yield,with a lower N_(2)O emission factor(EF)from fertilizer N of 0.95%.Nitrogen fertilization considerably raised the AOB,nirS,nirK and nosZ gene abundances,in comparison to the Control treatment.Moreover,the substitution of organic manure for inorganic N fertilizer significantly decreased AOB and nirS gene abundances and increased nosZ gene abundance.The AOB responded to N fertilization more sensitively than the AOA.Total N_(2)O emissions significantly correlated positively with AOB and nirS gene abundances while having a negative correlation with nosZ gene abundance and the nosZ/nirS ratio across N-fertilized plots.In summary,we conclude that organic manure substitution for inorganic N fertilizer decreased soil N_(2)O emissions primarily by changing the soil NO_(3)^(−)-N,pH and DOC levels,thus inhibiting the activities of ammonia oxidation in nitrification and nitrite reduction in denitrification,and strengthening N_(2)O reduction in denitrification from water-saving rice paddies.

Comparative effects of composted organic waste and inorganic fertilizer on nitrate leachate from the farm soils of northern Guam

The purpose of this study was to quantify the release of nitrate into vadose zone as well as the nitrogen-holding capacity of compost applied on calcareous soils of northern Guam amended with both inorganic fertilizer and composted organic waste,to examine potentially adverse effect of these nutrient materials to groundwater quality.Three different nitrogen levels each of the composted organic waste and the inorganic fertilizer were applied to corn(maize)(farmland)study plots for three consecutive seasons,two dry and one rainy season.During each season,soil organic matter(SOM,w/w%)content and carbon-nitrogen ratio(C/N,w/w%),were determined for analysis of the nitrogen-holding capacity of these calcareous soils.Nitrate levels in soil pore water were also determined for study of potential groundwater contamination.For three seasons,compost plots showed higher SOM%contents and lower C/N%than fertilizer plots.That is,compost-treated soils showed higher SOM content and adsorbed more nitrogen under normalized soil mass than did fertilizer-treated soils.Nitrate levels in pore water were generally higher on compost plots during early stages of corn but were generally higher on fertilizer plots during active leaf,tasseling,and maturity stages.During the rainy season,nitrate leachate seemed to increase,but this trend could not be confirmed because rainfall amounts were not measured.Overall,composted organic waste proved to be a good amendment for soil productivity and agricultural sustainability while reducing nitrate leachate from northern Guam farmland.

Growth, Yield and Yield Characteristics of Three Pepper Cultivars to Fertilizers Application in the Mount Cameroon Region

This study determined the effects of organic and inorganic fertilizers on growth and yield of three pepper cultivars in the Mount Cameroon Region. Pepper seedlings were treated with six treatments (T1 = no fertilizers (control), T2 = 250 Kg/ha NPK 20:10:10, T3 = 350 Kg/ha NPK 20:10:10, T4 = 450 Kg/ha NPK 20:10:10, T5 = 20 Mg/ha poultry manure (PM), T6 = 30 Mg/ha PM). The effects of fertilizers had a significant effect on vegetative growth and yield of Capsicum chinense cv. “Big Sun” plants had the maximum plant height (39.09 cm) and mean collar diameter (6.02 mm) in plots treated with poultry manure at 20 Mg/ha (T5) and 30 Mg/ha (T6) respectively. The highest yield (33.63 Mg/ha) was in Capsicum chinense cv. Safi plants supplied with 30 Mg/ha poultry manure (T6) and the lowest yield (10.44 Mg/ha) was in Capsicum frutescens in control plots (T1). The highest fruit diameter (11.08 mm) and longest fruits (18.39 mm) were observed in Capsicum chinense cv. Big Sun plants supplied with 30 Mg/ha poultry manure. Capsicum frutescens fruits had the highest concentration of N (2.39%), P (0.42%) and K (3.06%). Essential oils analysis shows that there were 179 essential oils from pepper fruits of Capsicum chinense cv. Safi, with major essential oil been 3,4-dimethyl-benzaldehyde and main essential oils were alpha Terpinene and alpha Pinene based on their abundance. Fruits of Capsicum chinense cv. Big Sun had two-hundred and eight essential oils with major essential oil been 1,3,5-Trimethyl-1-H-pyrazol-4-amine and main essential oils were Caryophyllene oxide and Cymene, based on their abundance. Fruits of Capsicum frutescens had one-hundred and eighty-one essential oils with major essential oil been Limonene, and main essential oils were Carvacrol and D-limonene with respect to abundance. Results show that 20 Mg/ha poultry manure was the most cost effective in the production of pepper in Buea Cameroon.

Co-application of compost or inorganic NPK fertilizer with biochar influences soil quality,grain yield and net income of rice

Most agricultural soils in sub-Saharan Africa are degraded,compromising the grain yield of rice and farmers return on investment.A 3-year field study was undertaken to explore the effect of the application of compost or inorganic NPK fertilizer applied alone or in combination with biochar on soil quality,grain yield of rice and net income.The five treatments were laid out using a randomized complete block design with four replications.The treatments were applied to supply approximately 75 kg N ha–1.The best fertilizer input was compost+biochar which resulted in the greatest improvement in soil physico-chemical properties by reducing bulk density and increasing porosity and moisture retention,organic matter content,percent nitrogen,available phosphorus and cation exchange capacity.Apart from treatment with inorganic fertilizer alone,treated soils showed a decrease in pH.Bacterial and fungal counts and basal respiration decreased in soils in the following order:compost+biochar>compost only>inorganic NPK fertilizer+biochar>inorganic NPK fertilizer>control.The increase in pooled grain yield and net income in response to treatment followed the order:compost+biochar>NPK+biochar>NPK>compost>control.The findings suggest that the use of compost or NPK alone might improve soil quality and increase grain yield and net income,but it is greatly recommended to co-apply these fertilisers with biochar.

The effects of straw‑returning and inorganic K fertilizer on the carbon–nitrogen balance and reproductive growth of cotton

Background:Many studies have indicated that straw-returning could meet part or even all of the potassium(K)demand for crop growth in the field,but few have compared the effects of crop straw as K source and inorganic K fertilizer on carbon–nitrogen(C–N)balance of cotton and the reproductive growth.To address this,field experiments were conducted using the cotton cultivar,Siza 3,under there treatments(CK as control group one,no crop straw and inorganic K fertilizer were applied;K150 as control group two,150 kg·ha^(-1) of K2O was applied;and W9000,9000 kg·ha^(-1) wheat straw,which could provide K2O about 150 kg·ha^(-1),was incorporated into soil).Results:Although the final reproductive organ biomass did not differ between W9000 and K150,W9000 had a higher ratio of reproductive organ biomass to total biomass(RRT),suggesting that straw-returning was more conducive to the allocation of biomass to reproductive organs.The theoretical maximum biomass of reproductive organ was higher,but the average and maximum accumulation rates of reproductive organ biomass were 2.8%∼8.3%and 2.5%∼8.2%lower under W9000 than K150.Also,the duration of rapid-accumulation period for reproductive organ biomass(T)was 2.0∼2.8 d longer under W9000 than K150,which was a reason for the higher RRT under W9000.Straw-returning altered the dynamics of leaf K with the growth period,so that W9000 had a more drastic effect on leaf C metabolism than K150.Consequently,lower soluble sugar/free amino acid and C/N ratios were measured under W9000 than K150 at boll-setting(BSS)and boll-opening(BOS)stages.Higher leaf net photosynthetic rate,sucrose phosphate synthase and sucrose synthase activities,and lower acid invertase activity were observed under W9000 than K150 at BSS and BOS and these were more conducive to sucrose accumulation.However,less sucrose was measured under W9000 than K150 at these stages.This should be because straw-returning promoted the assimilate transport capacity when compared with inorganic K fertilizer application,which also explained the higher RRT under W9000 than K150.The lower acid invertase activity under W9000 inhibited the conversion of sucrose to other sugars,hence lower contents of soluble sugar and starch were measured under W9000 than K150.Conclusion:Under low K condition,crop straw as K source can increase the assimilate transport from source to sink,leading to lower C/N ratio in leaf and higher allocation of biomass to reproductive organs than inorganic K fertilizer.

The Application of Fertilizer and AMF Promotes Growth and Reduces the Cadmium and Lead Contents of Ryegrass (Lolium multiflorum L.) in a Copper Mining Area

Heavy metal-polluted soil was collected from the Pulang copper mine in Shangri-La City,Yunnan Province,Southwest China.The effects of fertilizer(organic and inorganic)and arbuscular mycorrhizal fungi(AMF)on ryegrass(Lolium multiflorum L.)growth,root morphology,mineral nutrition and cadmium(Cd)and lead(Pb)contents were investigated by pot experiments.The results showed that both fertilizer and AMF significantly ameliorated the root morphology and mineral nutrition,reduced the Cd and Pb contents,and promoted the growth of ryegrass.Among all treatments,the combined application of organic–inorganic compound fertilizer with AMF had the highest effect,resulting in increases in root length,surface area and branch number by 2.3,1.1,and 3.9 times,respectively;an 88%increase in plant biomass;a nitrogen content increase of 2.3 and 1.2 times,and phosphorus content increase of 62%and 68%in shoots and roots,respectively was also recorded as well as decreases in Cd content by 34%and 62%and Pb content by 47%and 34%in shoots and roots,respectively.Twofactor analysis showed that both fertilizer and AMF significantly promoted ryegrass growth(plant height,biomass,chlorophyll content,root length,nitrogen and phosphorus content)and reduced the Cd and Pb contents in roots,and there was a synergistic effect between them.Moreover,the nitrogen and phosphorus contents were very significantly positively correlated with the shoot and root biomasses but very significantly negatively correlated with the Cd and Pb contents of ryegrass.Thus,the application of fertilizer and AMF synergistically improved ryegrass growth on polluted soils in the copper mining area.

RETRACTED:Effect of Long-Term Inorganic Fertilization on Diversity and Abundance of Bacterial and Archaeal Communities at Tillage in Irrigated Rice Field

Short Retraction Notice The paper does not meet the standards of "Advances in Bioscience and Biotechnology". This article has been retracted to straighten the academic record. In making this decision the Editorial Board follows COPE's Retraction Guidelines. The aim is to promote the circulation of scientific research by offering an ideal research publication platform with due consideration of internationally accepted standards on publication ethics. The Editorial Board would like to extend its sincere apologies for any inconvenience this retraction may have caused. Editor guiding this retraction: Prof. Abass Alavi (EiC of ABB). Please see the article page for more details. The full retraction notice in PDF is preceding the original paper which is marked "RETRACTED".

Calcium carbonate promotes the formation and stability of soil macroaggregates in mining areas of China

We studied changes in the concentrations of aggregate-cementing agents after different reclamation times and with different fertilization regimes,as well as the formation mechanism of aggregates in reclaimed soil,to provide a theoretical basis for rapid reclamation of soil fertility in the subsidence area of coal mines in Shanxi Province,China.In this study,soil samples of 0–20 cm depth were collected from four fertilization treatments of a longterm experiment started in 2008:no fertilizer (CK),inorganic fertilizer (NPK),chicken manure compost (M),and50%inorganic fertilizer plus 50%chicken manure compost (MNPK).The concentrations of cementing agents and changes in soil aggregate size distribution and stability were analysed.The results showed that the formation of>2 mm aggregates,the aggregate mean weight diameter (MWD),and the proportion of>0.25 mm water-stable aggregates (WR_(0.25)) increased significantly after 6 and 11 years of reclamation.The concentration of organic cementing agents tended to increase with reclamation time,whereas free iron oxide (Fed) and free aluminium oxide(Ald) concentrations initially increased but then decreased.In general,the MNPK treatment signi?cantly increased the concentrations of organic cementing agents and CaCO_(3),and CaCO_(3) increased by 60.4%at 11 years after reclamation.Additionally,CaCO_(3) had the greatest effect on the stability of aggregates,promoting the formation of>0.25 mm aggregates and accounting for 54.4%of the variance in the proportion and stability of the aggregates.It was concluded that long-term reclamation is bene?cial for improving soil structure.The MNPK treatment was the most effective measure for increasing maize grain yield and concentration of organic cementing agents and CaCO_(3).

Effect of N Fertilizers on Root Growth and Endogenous Hormones in Strawberry

Endogenous hormones play an important role in the growth and development of roots. The objective of this research was to study the effect of four types of N fertilizers on the root growth of strawberry (Fragaria ananassa Duchesne) and the endogenous enzymes of indole-3-acetic acid (IAA), abscisic acid (ABA), and isopentenyl adenosine (iPA) in its roots and leaves using enzyme-linked immunosorbent assay. Application of all types of N fertilizers significantly depressed (P ≤ 0.05) root growth at 20 d after transplanting. Application of organic-inorganic fertilizer (OIF) as basal fertilizer had a significant negative effect (P ≤ 0.05) on root growth. The application of OIF and urea lowered the lateral root frequency in strawberry plants at 60 d (P ≤ 0.05) compared with the application of two organic fertilizers (OFA and OFB) and the control (CK). With the fertilizer treatments, there were the same concentrations of IAA and ABA in both roots and leaves at the initial growth stage (20 d), lower levels of IAA and ABA at the later stage (60 d), and higher iPA levels at all seedling stages as compared to those of CK. Thus, changes in the concentrations of endogenous phytohormones in strawberry plants could be responsible for the morphological changes of roots due to fertilization.

The effect of long-term application of nitrogen-rich fertilizers on soil resistome:A study of conventional and organic cropping systems

Metagenomic studies of various soil environments have previously revealed the widespread distribution of antibiotic resistance genes(ARGs)around the globe.In this study,we applied shotgun metagenomics to investigate differences in microbial communities and resistomes in Chernozem soils that have been under long-term organic and conventional cropping practices.The organic cropping system was seeded with Triticum spelta without any fertilizer.The conventional cropping system was seeded with Tríticum durum Desf and used mineral fertilizer(NPK),that resulted in an increased amount of total and available carbon and nitrogen in soils.Across all samples,we identified a total of 21 ARG classes,among which the dominant were vancomycin,tetracycline and multidrug.Profiling of soil microbial communities revealed differences between the studied fields in the relative abundances of 14 and 53 genera in topsoil and subsoil,respectively.Correlation analysis showed significant correlations(positive and negative)among 18 genera and 6 ARGs,as well as between these ARGs and some chemical properties of soils.The analysis of metagenome-assembled genomes revealed that Nitrospirota,Thermoproteota,Actinobacteriota and Binatota phyla of archaea and bacteria serve as hosts for glycopeptide and fluoroquinolone/tetracycline ARGs.Collectively,the data obtained enrich knowledge about the consequences of human agricultural activities in terms of soil microbiome modification and highlight the role of nitrogen cycling taxa,including uncultivated genera,in the formation of soil resistome.

Dynamics of Microbial Biomass in a Rainfed Soil Under Wheat Cultivation

A pot experiment was conducted to determine the dynamics of soil microbial biomass in a rainfed soil under wheat cultivation at the University of Arid Agriculture, Rawalpindi, Pakistan. The treatments applied were: 1) a control (CK), 2) NPK (0.44-0.26-0.18 g pot-1), 3) farmyard manure (FYM, 110 g pot-1), 4)poultry manure (PM, 110 g pot-1), 5) FYM (110 g pot-1) + NPK (0.44-0.26-0.18 g pot-1), 6) poultry manure (PM, 110 g pot-1) + NPK (0.44-0.26-0.18 g pot-1), 7) FYM (110 g pot-1) + NPK(S) (0.44-0.26-0.18 g pot-1, one half of the NPK at sowing and the other half one month after sowing), and 8) PM (110 g pot-1) + NPK(S) (0.44-0.26-0.18 g pot-1, one half of the NPK applied at sowing and the other half one month after sowing). The experiment was laid out using a completely randomized design with three replications. Microbial biomass C, N and P contents increased continuously from the beginning of the experiment up to the three-leaf stage. A slight decline was observed at the tillering stage in all treatments except with the organic manures + NPK(S) treatments. After tillering there was an increase in all treatments to the recorded maximum point at the full heading stage in all treatments except with the organic manures + NPK(S) treatments. In the FYM + NPK(S) and PM + NPK(S) treatments; however, there was a continuous increase in microbial biomass up to the heading stage. At the harvesting stage a sharp decline was noted in all treatments. The C:N ratio of microbial biomass in tested soil ranged from 7.8 to 11.3, while C:P ratio of microbial biomass in the tested soil ranged from 22.6 to 35.1 throughout all growth stages of the wheat crop.

Changes of Organic Matter, N, P and K Content of Soils in Red Soil Areas Under Long-Term Experiment

The content of organic matter （OM）, nitrogen （N）, phosphorus （P）, and potassium （K） in the three selected soils, including Calcaric Purpli-Orthic Primosols （purple sand-shale parent material）, Dystric Turbi-Anthric Primosols （quaternary red clay parent material） and Typic Udi-Sandic Primosols （granite parent material） were studied under a long-term experiment by using crop straw and inorganic fertilizers at the Hunan Red Soil Experiment Station of Chinese Academy of Agricultural Sciences. The results showed that the contents of OM, N, P and K in the three selected soils increased after 23 years application of crop straw and inorganic fertilizers, but the contents increased much less when crop straw or inorganic fertilizers was applied alone. The nutrient contents in the three soils developed from granite changed more remarkably than those in the soil derived from quaternary red clay and purple sand-shale. It was also found that the contents of OM, N, and P increased slightly in the treatments without applying fertilizers or returning the crop straw to the root bed. Combined application of inorganic fertilizers and crop straw could remarkably increase the contents of OM, alkalihydrolyzable N and available K, the positive correlation between application of organic fertilizers and increase of OM in soil did not always happened, it provided evidence for the relation between appropriate C/N ratio and accumulation of OM in soil. The increase of nutrient content was influenced by the soil properties. By comparing the contents of nutrient in 0-20 cm depth in the three different soils, it was concluded that the most increases of OM, alkali-hydrolyzable N, and available P were observed in Typic Udi-Sandic Primosols with the average increase by 3.03, 27.38, and 21.73 mg kg^-1, respectively. The available K increased in Dystric Turbi-Anthric Primosols with the average increase by 25.82 mg kg^-1, while it decreased in Calcaric Purpli-Orthic Primosols and Typic Udi-Sandic Primosols. It was concluded that the application of inorganic fertilizer and crop straw was important to improve the soil fertility for all of three selected soils. The straw return to the field had played a significant role for enhancement of the soil quality in the study areas. The application of inorganic fertilizer combined with the straw return to the fields could remarkably improve the soil fertility.