Light-saturation curves of Dicrateria inornata at various temperatures were determined by using 14C-la belling technique. Kinetics of light-intensity adapation was also analyzed in combination with Chl.a determination...Light-saturation curves of Dicrateria inornata at various temperatures were determined by using 14C-la belling technique. Kinetics of light-intensity adapation was also analyzed in combination with Chl.a determination and cell enumeration measurements. The response of cellular Chl. a in light transition processes can be appoximately described with the first order kinetic model. The model between temperature and maximum growth rate was established by using the Arrhenius equation.展开更多
本研究以舟山产裸五角瓜参为对象,研究了体壁组织的基本组成成分、酸溶与胃酶促溶胶原提取率、提取胶原蛋白特性以及其体外抗氧化性。结果表明:裸五角瓜参蛋白质含量较高,粗蛋白含量达到18.56%,且以胶原蛋白为主,该胶原蛋白经酸提法和...本研究以舟山产裸五角瓜参为对象,研究了体壁组织的基本组成成分、酸溶与胃酶促溶胶原提取率、提取胶原蛋白特性以及其体外抗氧化性。结果表明:裸五角瓜参蛋白质含量较高,粗蛋白含量达到18.56%,且以胶原蛋白为主,该胶原蛋白经酸提法和酶提法处理后具有典型的水产品胶原蛋白特性,2%为Na Cl最佳添加浓度;电泳实验表明该胶原蛋白的链和β链分子量分别为98 k U和102 k U;此外,两种胶原蛋白还具有一定的抗氧化性,但其抗氧化能力较弱。展开更多
We studied the effects of nitrogen （N） and phosphorus （P） supply on Fv/Fm （maximal quantum yield of photosystem Ⅱ） in the algae Chaetoceros debilis, Dicrateria inornata, Platymonas subcordiformis and Heterosigm...We studied the effects of nitrogen （N） and phosphorus （P） supply on Fv/Fm （maximal quantum yield of photosystem Ⅱ） in the algae Chaetoceros debilis, Dicrateria inornata, Platymonas subcordiformis and Heterosigma akashiwo to determine the sensitivity of Fv/Fm to P-limitation of the four species. Obvious decrease of Fv/Fm value was monitored in periods of P-depletion, the decrease showing different magnitudes among algal species. A more clear decrease of Fv/Fm in Platymonas subcordiformis and Heterosigma akashiwo was observed, compared with that in Chaetoceros debilis and Dicrateria inornata. After the re-supply of P, the index Fv/Fm of the four species all recovered quickly in 24h. Cell division was maintained and chlorophyll content continued to increase until the end of the experiment in Platymonas subcordiformis and Heterosigma akashiwo, while in Chaetoceros debilis and Dicrateria inornata, the division stopped in the later period when the growth of organisms came into the stationary phase. The most obvious respond of chlorophyll content to P-additions was observed in Heterosigma akashiwo culture in 24 h. The sensitivity of Fv/Fm to nutrient limitations is different among algal species, and much should be done to improve the application of this index.展开更多
文摘Light-saturation curves of Dicrateria inornata at various temperatures were determined by using 14C-la belling technique. Kinetics of light-intensity adapation was also analyzed in combination with Chl.a determination and cell enumeration measurements. The response of cellular Chl. a in light transition processes can be appoximately described with the first order kinetic model. The model between temperature and maximum growth rate was established by using the Arrhenius equation.
文摘本研究以舟山产裸五角瓜参为对象,研究了体壁组织的基本组成成分、酸溶与胃酶促溶胶原提取率、提取胶原蛋白特性以及其体外抗氧化性。结果表明:裸五角瓜参蛋白质含量较高,粗蛋白含量达到18.56%,且以胶原蛋白为主,该胶原蛋白经酸提法和酶提法处理后具有典型的水产品胶原蛋白特性,2%为Na Cl最佳添加浓度;电泳实验表明该胶原蛋白的链和β链分子量分别为98 k U和102 k U;此外,两种胶原蛋白还具有一定的抗氧化性,但其抗氧化能力较弱。
基金support from the National Natural Science Foundation of China (No. 41076065)the National Basic Research Program of China (973 Program) (No. 2010CB428701)
文摘We studied the effects of nitrogen （N） and phosphorus （P） supply on Fv/Fm （maximal quantum yield of photosystem Ⅱ） in the algae Chaetoceros debilis, Dicrateria inornata, Platymonas subcordiformis and Heterosigma akashiwo to determine the sensitivity of Fv/Fm to P-limitation of the four species. Obvious decrease of Fv/Fm value was monitored in periods of P-depletion, the decrease showing different magnitudes among algal species. A more clear decrease of Fv/Fm in Platymonas subcordiformis and Heterosigma akashiwo was observed, compared with that in Chaetoceros debilis and Dicrateria inornata. After the re-supply of P, the index Fv/Fm of the four species all recovered quickly in 24h. Cell division was maintained and chlorophyll content continued to increase until the end of the experiment in Platymonas subcordiformis and Heterosigma akashiwo, while in Chaetoceros debilis and Dicrateria inornata, the division stopped in the later period when the growth of organisms came into the stationary phase. The most obvious respond of chlorophyll content to P-additions was observed in Heterosigma akashiwo culture in 24 h. The sensitivity of Fv/Fm to nutrient limitations is different among algal species, and much should be done to improve the application of this index.
