This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low ...This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality.By studying molecular markers of fat content in sheep muscles,we searched for polymorphic loci related to fat content in sheep muscles on the ANGPTL4 gene.Among the 9 polymorphic loci retrieved,ANGPTL4 was found.Two polymorphic sites,C724 A and A601 G,were significantly associated with intramuscular fat in mutton.In summary,these two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.展开更多
[ Objective] The paper was to investigate the degradation and deposition changes of inosine monophosphate （IMP） and its metabolites in muscle of lean pigs and crossbreeding pigs under cold storage condition at 4℃. ...[ Objective] The paper was to investigate the degradation and deposition changes of inosine monophosphate （IMP） and its metabolites in muscle of lean pigs and crossbreeding pigs under cold storage condition at 4℃. [ Method] The contents of IMP and its metabolites in longissimus dorsi of lean pigs and crossbreeding pigs were determined by HPLC method. [ Result] Duroc, Landrace, Yorkshire and DLY pigs shared the same degradation and deposition pattern on IMP, inosine and hypoxanthine. On the second day of cold storage, the content of IMP reached the maximum value, which increased by 0.4% - 15.32% compared with the first day, and then it started to decline. On the fourth day of cold storage, the content of IMP significantly reduced by 23.81% - 39.06% than that on the second day. On the fifth and sixth day of cold storage, the content of IMP kept on falling down slowly and maintained around 1.0 mg/g. While the contents of inosine and hypoxanthine showed an increasing tendency with the extension of cold storage time. [ Conclusion ] Lean pigs Duroc, Landraee and Yorkshire and DLY three- way crossbreeding pigs shared the same degradation and deposition pattern on IMP and its metabolites. With the extension of cold storage, the content of IMP first increased then gradually decreased; while inosine and hypoxanthine gradually increased, and the difference among breeds was not significant.展开更多
Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine de...Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine degradation rate reached 36.3%at 109 CFU/mL for 24 h.LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L.rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin.The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts(CFE).L.rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase(XOD)inhibition and ATP-binding cassette subfamily G member 2(ABCG2)promotion,both of which are responsible for uric acid(UA)synthesis and secretion in HepG2 and Caco2 cells,respectively.The in vivo results showed that the serum UA level decreased from 236.28 to 149.28μmol/L after 8 weeks of oral administration of L.rhamnosus Fmb14 in inosine-induced hyperuricemia model mice.Our results revealed that L.rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.展开更多
Inosine monophosphate(IMP),as a critical umami substance,is one of the most important indicators for evaluating the quality of meat products.Here,a sensitive electrochemiluminescence(ECL)biosensor based on graphdiyne(...Inosine monophosphate(IMP),as a critical umami substance,is one of the most important indicators for evaluating the quality of meat products.Here,a sensitive electrochemiluminescence(ECL)biosensor based on graphdiyne(GDY)/AuNPs/luminol nanocomposites was constructed to detect IMP.The GDY/AuNPs/luminol nanocomposites were synthesized by using simple one-pot method.GDY utilized its 2D framework to disperse and fix gold nanoparticles,which inhibited the agglomeration of gold nanoparticles and greatly improved its stability and catalytic properties.Importantly,GDY/AuNPs/luminol nanocomposites showed excellent catalytic ability and superior ECL activity towards luminol-H_(2)O_(2) systems due to the synergistic effect of GDY and AuNPs.Under optimal conditions,the prepared biosensor exhibited a wide linear range from 0.01 g/L to 20 g/L,a satisfactory limit detection of 0.0013 g/L,as well as an excellent specificity.Moreover,we carried out the precise analysis of IMP in actual meat samples with acceptable results compared to the liquid chromatography.We believe that this work could offer an efficient ECL platform for accurate and reliable report of IMP levels,which is significant for maintaining food quality and safety.展开更多
文摘This study was conducted to speed up the process of improving the quality of lamb breeds in China,and to solve the problems that restrict the development of lamb breeds in China,such as low quality of lamb breeds,low meat production rate,and poor meat quality.By studying molecular markers of fat content in sheep muscles,we searched for polymorphic loci related to fat content in sheep muscles on the ANGPTL4 gene.Among the 9 polymorphic loci retrieved,ANGPTL4 was found.Two polymorphic sites,C724 A and A601 G,were significantly associated with intramuscular fat in mutton.In summary,these two molecular markers can be used as a molecular marker reference when breeding high-quality meat or hair and meat using German Merino sheep breeds.
基金Supported by National Natural Science Foundation of China(31501928)Youth Fund Project of Shandong Academy of Agricultural Sciences(2014QNM41)+1 种基金Science and Technology Development Program of Shandong Province(2015GNC111011)Natural Science Foundation of Shandong Province(ZR2015CM007)
文摘[ Objective] The paper was to investigate the degradation and deposition changes of inosine monophosphate （IMP） and its metabolites in muscle of lean pigs and crossbreeding pigs under cold storage condition at 4℃. [ Method] The contents of IMP and its metabolites in longissimus dorsi of lean pigs and crossbreeding pigs were determined by HPLC method. [ Result] Duroc, Landrace, Yorkshire and DLY pigs shared the same degradation and deposition pattern on IMP, inosine and hypoxanthine. On the second day of cold storage, the content of IMP reached the maximum value, which increased by 0.4% - 15.32% compared with the first day, and then it started to decline. On the fourth day of cold storage, the content of IMP significantly reduced by 23.81% - 39.06% than that on the second day. On the fifth and sixth day of cold storage, the content of IMP kept on falling down slowly and maintained around 1.0 mg/g. While the contents of inosine and hypoxanthine showed an increasing tendency with the extension of cold storage time. [ Conclusion ] Lean pigs Duroc, Landraee and Yorkshire and DLY three- way crossbreeding pigs shared the same degradation and deposition pattern on IMP and its metabolites. With the extension of cold storage, the content of IMP first increased then gradually decreased; while inosine and hypoxanthine gradually increased, and the difference among breeds was not significant.
基金the National Natural Science Foundation of China(32072182).
文摘Hyperuricemia is a critical threat to human health,and a high inosine diet can increase the prevalence of it.Lacticaseibacillus rhamnosus Fmb14 was isolated from traditional fermented Chinese yogurt,and its inosine degradation rate reached 36.3%at 109 CFU/mL for 24 h.LC-MS analysis revealed that high concentrations of inosine could activate compensatory metabolic pathways of L.rhamnosus Fmb14 to catalyse inosine as an energy source and produce intracellular folic acid and riboflavin.The contents of folic acid and riboflavin were 6.0 and 4.3 fold increased after inosine treatment in the cell-free extracts(CFE).L.rhamnosus Fmb14 CFE treatment ameliorates hyperuricemia through xanthine oxidase(XOD)inhibition and ATP-binding cassette subfamily G member 2(ABCG2)promotion,both of which are responsible for uric acid(UA)synthesis and secretion in HepG2 and Caco2 cells,respectively.The in vivo results showed that the serum UA level decreased from 236.28 to 149.28μmol/L after 8 weeks of oral administration of L.rhamnosus Fmb14 in inosine-induced hyperuricemia model mice.Our results revealed that L.rhamnosus Fmb14 has a potential as a biological therapeutic agent in hyperuricemia prevention.
基金supported by The National Natural Science Foundation of China(31972198,31622042)The National Key R&D Program of China(2016YFD0400803,2016YFD0401501).
文摘Inosine monophosphate(IMP),as a critical umami substance,is one of the most important indicators for evaluating the quality of meat products.Here,a sensitive electrochemiluminescence(ECL)biosensor based on graphdiyne(GDY)/AuNPs/luminol nanocomposites was constructed to detect IMP.The GDY/AuNPs/luminol nanocomposites were synthesized by using simple one-pot method.GDY utilized its 2D framework to disperse and fix gold nanoparticles,which inhibited the agglomeration of gold nanoparticles and greatly improved its stability and catalytic properties.Importantly,GDY/AuNPs/luminol nanocomposites showed excellent catalytic ability and superior ECL activity towards luminol-H_(2)O_(2) systems due to the synergistic effect of GDY and AuNPs.Under optimal conditions,the prepared biosensor exhibited a wide linear range from 0.01 g/L to 20 g/L,a satisfactory limit detection of 0.0013 g/L,as well as an excellent specificity.Moreover,we carried out the precise analysis of IMP in actual meat samples with acceptable results compared to the liquid chromatography.We believe that this work could offer an efficient ECL platform for accurate and reliable report of IMP levels,which is significant for maintaining food quality and safety.
