BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine...BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.展开更多
The most serious potential hazard of cowpea product is the pesticide residue for controlling such pests as thrips and cowpea pod borer. To explore a safe and efficient mode of cowpea production that is fit for climati...The most serious potential hazard of cowpea product is the pesticide residue for controlling such pests as thrips and cowpea pod borer. To explore a safe and efficient mode of cowpea production that is fit for climatic characteristics of Hainan, ensure the safety of cowpea supply, minimize the use of pesticides, and control pests in physical means, insect-proof nets are applied in cowpea cultivation. We set 5-mm, 2.5-mm and 1.67-ram insect-proof nets as three treatments and open filed cultivation as CK to explore efficiency of insect-preof net in cowpoa production in terms of insect-proof efficiency, biological characters and commodity properties and yield of cowpea. The results showed that insect-proof cultivation was efficient in controlling pests, especially the 2.5-mm net showed the optimal effect for cowpea production.展开更多
[Objectives] This study was conducted to screen small watermelon varieties suitable for spring greenhouses. [Methods] The field traits, yield traits, quality traits and commodity of 6 new red-flesh watermelon varietie...[Objectives] This study was conducted to screen small watermelon varieties suitable for spring greenhouses. [Methods] The field traits, yield traits, quality traits and commodity of 6 new red-flesh watermelon varieties were compared. [Results] "Jingmei 3 K" and "Sumeng No.5" had obvious advantages in quality traits, yield traits, and commodity traits. The yield reached 62 163.3 and 63 733.5 kg/hm2, respectively;the single melon weights reached 2.16 and 2.21 kg, respectively;and the central sugar content reached 13.2% and 13.1%, respectively. Meanwhile, the juice contents were high, and the taste was sweet and refreshing. They are suitable for planting under the condition of substrate cultivation technology. [Conclusions] This study provides a theoretical basis for large-area substrate cultivation and production.展开更多
Many dwellings in warm-humid climates attain a comfortable environment by natural ventilation.The opening of exterior windows for ventilation allows the entry of insects along with the breeze.As a remedy,occupants ins...Many dwellings in warm-humid climates attain a comfortable environment by natural ventilation.The opening of exterior windows for ventilation allows the entry of insects along with the breeze.As a remedy,occupants install insect-proof screens on windows resulting in reduced airflow into the interior.This study attempts to evaluate the air change effectiveness and thermal comfort in a residential kitchen with insect-proof screens.A kitchen with insect-proof screens on the windows is compared with a case without insect-proof screens.Numerical simulation was conducted using ANSYS Fluent 2019 R2.The insect-proof screen is modelled as a porous media.The air velocity and temperature measurements were validated by measurements in a real scenario.The presence of insect-proof screens reduced the air velocity inside the space by 82%.However,the airflow pattern in the case with screens was more uniformly distributed.The mean age of the air was considerably higher in the case with insect-proof screens,which in turn resulted in a reduced ACE.The presence of an insect-proof screen resulted in a Predicted Mean Vote(PMV)of 2.79 indicating a‘hot’sensation,whereas in the other case,the comfort vote is only 1.93 indicating a‘warm’sensation.The presence of insect-proof screens on windows reduced the air velocity and ventilation efficiency,contributing to increased thermal discomfort in the kitchen.展开更多
基金the Chongqing Talent Plan“Contract System”Project,No.cstc2022ycjh-bgzxm0137Natural Science Foundation of Chongqing,No.CSTB2024NSCQ-MSX0003the Ethics Committee of Chongqing General Hospital.The ethics review number:No.KY S2022-045-01.
文摘BACKGROUND The frequent suboptimal efficacy of endoscopic ultrasound-guided fine-needle biopsy(EUS-FNB)to culture pancreatic cancer(PC)organoids(PCOs)poses a major challenge in the advancement of personalized medicine for advanced PC.AIM To explore how to obtain appropriate puncture tissues from EUS-FNB and optimize the strategy for efficiently constructing PCOs,providing an efficient tool for the advancement of personalized medicine.METHODS Patients who underwent EUS-FNB for the diagnosis of PC tissue were prospectively enrolled.We refined the endoscopic biopsy procedures and organoid cultivation techniques.All tissue specimens verified by on-site pathological assessment were cultured in a semi-suspended medium in a microfluidic environment.We assessed differences in PCOs cultured beyond and below five generations examining patient demographics,specimen and organoid attributes,and the sensitivity of organoids to a panel of clinical drugs through cell viability assays.RESULTS In this study,16 patients with PC were recruited,one sample was excluded because onsite cytopathology showed no tumor cells.Successful organoid generation occurred in 93.3%(14 of 15)of the EUS-FNB specimens,with 60%(9 of 15)sustaining over five generations.Among these patients,those with a history of diabetes,familial cancer,or larger tumors exhibited enhanced PCO expandability.The key factors influencing longterm PCOs expansion included initial needle sample quality(P=0.005),rapid initiation of organoid culture postisolation(P≤0.001),and high organoid activity(P=0.031).Drug sensitivity analysis revealed a partial response in two patients following therapeutic intervention and surgery and stable disease in four patients,indicating a moderate correlation between organoid response and clinical outcomes.CONCLUSION Optimal initial needle sampling,rapid and precise biopsy sample processing,process isolated samples as soon as possible,and sufficient cellular material are crucial for successful cultivating PCOs.High organoid activity is an important factor in maintaining their long-term expansion,which is essential for shortening the time of drug sensitivity analysis and is the basis of PC research.
文摘The most serious potential hazard of cowpea product is the pesticide residue for controlling such pests as thrips and cowpea pod borer. To explore a safe and efficient mode of cowpea production that is fit for climatic characteristics of Hainan, ensure the safety of cowpea supply, minimize the use of pesticides, and control pests in physical means, insect-proof nets are applied in cowpea cultivation. We set 5-mm, 2.5-mm and 1.67-ram insect-proof nets as three treatments and open filed cultivation as CK to explore efficiency of insect-preof net in cowpoa production in terms of insect-proof efficiency, biological characters and commodity properties and yield of cowpea. The results showed that insect-proof cultivation was efficient in controlling pests, especially the 2.5-mm net showed the optimal effect for cowpea production.
文摘[Objectives] This study was conducted to screen small watermelon varieties suitable for spring greenhouses. [Methods] The field traits, yield traits, quality traits and commodity of 6 new red-flesh watermelon varieties were compared. [Results] "Jingmei 3 K" and "Sumeng No.5" had obvious advantages in quality traits, yield traits, and commodity traits. The yield reached 62 163.3 and 63 733.5 kg/hm2, respectively;the single melon weights reached 2.16 and 2.21 kg, respectively;and the central sugar content reached 13.2% and 13.1%, respectively. Meanwhile, the juice contents were high, and the taste was sweet and refreshing. They are suitable for planting under the condition of substrate cultivation technology. [Conclusions] This study provides a theoretical basis for large-area substrate cultivation and production.
基金supported in part by the Department of Science and Technology,Ministry of Science and Technology,Government of India through the project-Community-Scale Energy Demand Reduction in India(CEDRI)under Grant DST/TMD/UK-BEE/2017/20.
文摘Many dwellings in warm-humid climates attain a comfortable environment by natural ventilation.The opening of exterior windows for ventilation allows the entry of insects along with the breeze.As a remedy,occupants install insect-proof screens on windows resulting in reduced airflow into the interior.This study attempts to evaluate the air change effectiveness and thermal comfort in a residential kitchen with insect-proof screens.A kitchen with insect-proof screens on the windows is compared with a case without insect-proof screens.Numerical simulation was conducted using ANSYS Fluent 2019 R2.The insect-proof screen is modelled as a porous media.The air velocity and temperature measurements were validated by measurements in a real scenario.The presence of insect-proof screens reduced the air velocity inside the space by 82%.However,the airflow pattern in the case with screens was more uniformly distributed.The mean age of the air was considerably higher in the case with insect-proof screens,which in turn resulted in a reduced ACE.The presence of an insect-proof screen resulted in a Predicted Mean Vote(PMV)of 2.79 indicating a‘hot’sensation,whereas in the other case,the comfort vote is only 1.93 indicating a‘warm’sensation.The presence of insect-proof screens on windows reduced the air velocity and ventilation efficiency,contributing to increased thermal discomfort in the kitchen.
