The artemisia weevil, Adosomus sp., has become an important wormwood （Artemisa ordosoca and A. sphaerocephala） pest in recent years in Ningxia Province, China. The number of larval instars should be determined befor...The artemisia weevil, Adosomus sp., has become an important wormwood （Artemisa ordosoca and A. sphaerocephala） pest in recent years in Ningxia Province, China. The number of larval instars should be determined before further studies on its life history, biology and integrate management of pest （IMP）. In this study, we measured the length and width of head capsule, based on frequency histograms and Dyar＇s law, from which we concluded that the larvae ofAdosomus sp. have four instars. Of the two variables measured, the width of head capsule provided the best division of instars. The average width of head capsule of the 1st instar larvae was 1.348±0.152 mm, the 2nd 2.109±0.199 mm, the 3rd 3.563±0.492 mm and the 4th 5.974±0.581 mm.展开更多
Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus ...Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus Kuala Lumpur.Methods:Twenty first instar Ae.aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried-leaf powder as their source of food.Combination of TMOF-Bti in rice husk formulation with the following weights viz 10,25,50 and 100 mg,respectively in duplicate was distributed in the buckets;while TMOF-Bti in wettable powder formulation each weighing viz 2,5,10 and 20 mg,respectively in duplicate was also placed in the buckets.The control buckets run in duplicate with 4 L of water and 20 first instar Ae.aegypti larvae.All buckets were covered with mosquito netting.Larval mortality was recorded after 24 hours and weekly for five weeks.A new batch of 20 1^(st) instar larvae Ae.aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder.The experiment was repeated for four times.Results:The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied.The TMOF(2%) + Bti(2%) had a good residual effect until the end of 3^(rd) week,TMOF(4%) + Bti(4%) until 4^(th) week,wettable powder TMOF(20%) + Bti(20%) until the third week.Condusions:From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.展开更多
The current experiment was conducted to find out the optimal conditions for mass rearing and developmental changes of Cyclosainsulana. The lab. conditions were maintained at （27±2）℃ and （65±5）% RH. The ...The current experiment was conducted to find out the optimal conditions for mass rearing and developmental changes of Cyclosainsulana. The lab. conditions were maintained at （27±2）℃ and （65±5）% RH. The clear perplex cages and natural diet consisting of the aphids, larvae of the house fly and larvae of drosophila were used for rearing. C. insulana took （123.12±7.26） days to develop from eggs to adults passing through eight instars under prevailing vivo conditions. The eggs were greenish white in color with average size of 0.57 mm ±0.034. The eggs spent （7.52±1.64） days in emergence. Maximum number of spiderlings survived at the 5th instar （84%） and minimum at the 1st instar （34%）. The measurements of different body parts including the total body length, cephalothorax and pedipalps of the both male and female C. insulana were done with the help of micrometer and presented as mean± SD. The measurements varied in the each developing instar. It was concluded that spiders were difficult to rear in the lab. conditions and each developing stage which was regarded as instars showed variations in size colors and body characteristics.展开更多
Apriona germari larvae were obtained via sample collection in woodland and indoor rearing. In this study, larval instars were divided by Dyar＇s law, and the results showed that the larval stage of A. germari comprise...Apriona germari larvae were obtained via sample collection in woodland and indoor rearing. In this study, larval instars were divided by Dyar＇s law, and the results showed that the larval stage of A. germari comprised six instars, while the width of head capsule was the best instar-dividing structure. On the basis of instar division, tubercle numbers on the transverse furrow downside of first abdominal dorsal ambulatory ampullae, seta numbers of abdominal telson and lateral seta numbers of mandible were observed and statistically calculated. The accuracy of instar-dividing was verified by comparing with the Dyar＇s law. The coincidence rate of two instar-dividing results was 90. 47%. It is approved that the above morphological features could be used in instar division ofA. germari larvae.展开更多
Polyphenol oxidase （PPO） is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO a metalloenzyme oxidase...Polyphenol oxidase （PPO） is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO a metalloenzyme oxidase, catalyzes the oxidation of o-diphenol to o-quinone. The present paper describes the effects of benzaldehyde and its p-substituted derivatives on the activity of PPO from the fifth instar of Pieris rapae L. PPO from the fifth instar of Pieris rapae L. was purified using ammonium sulfate fractionation and chromatography on Sephadex G-100. The enzyme kinetics was characterized using L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The results show that benzaldehyde, phydroxybenzaldehyde, p-chlorobenzaldehyde, and p-cyanobenzaldehyde can inhibit the PPO activity for the oxidation of L-DOPA. The inhibitor concentration leading to 50% activity lost, IC50, was estimated to be 5.90, 5.62, 2.83, and 2.91 mmol/L for the four tested inhibitors, respectively. Kinetic analyses show that the inhibitory effects of these compounds are reversible. Benzaldehyde, phydroxybenzaldehyde, and p-chlorobenzaldehyde are noncompetitive inhibitors while p-cyanobenzaldehyde is a mixed-type inhibitor. The inhibition constants were determined for all four inhibitors. p-chlorobenzaldehyde and p-cyanobenzaldehyde were more potent inhibitors than the other compounds. These results provide a basis for developing PPO inhibition-based pesticides.展开更多
基金supported by the National Project of ScienceTechnology for the 11th Five-Year Plan in China (Grant No. 2006BADO8A12)
文摘The artemisia weevil, Adosomus sp., has become an important wormwood （Artemisa ordosoca and A. sphaerocephala） pest in recent years in Ningxia Province, China. The number of larval instars should be determined before further studies on its life history, biology and integrate management of pest （IMP）. In this study, we measured the length and width of head capsule, based on frequency histograms and Dyar＇s law, from which we concluded that the larvae ofAdosomus sp. have four instars. Of the two variables measured, the width of head capsule provided the best division of instars. The average width of head capsule of the 1st instar larvae was 1.348±0.152 mm, the 2nd 2.109±0.199 mm, the 3rd 3.563±0.492 mm and the 4th 5.974±0.581 mm.
基金financially supported by Entogenex Industries Sdn.Bhd.,Malaysia(grant No.NN 001-2008)
文摘Objective:To evaluate the effectiveness and residual effects of trypsin modulating ecstatic factor-Bacillus thuringiensis israeliensis(TMOF-Bti) formulations against Aedes aegypti(Ae. aegypti)(L) larvae at UKM Campus Kuala Lumpur.Methods:Twenty first instar Ae.aegypti larvae were added in each bucket containing 4 L of water supplied with crushed dried-leaf powder as their source of food.Combination of TMOF-Bti in rice husk formulation with the following weights viz 10,25,50 and 100 mg,respectively in duplicate was distributed in the buckets;while TMOF-Bti in wettable powder formulation each weighing viz 2,5,10 and 20 mg,respectively in duplicate was also placed in the buckets.The control buckets run in duplicate with 4 L of water and 20 first instar Ae.aegypti larvae.All buckets were covered with mosquito netting.Larval mortality was recorded after 24 hours and weekly for five weeks.A new batch of 20 1^(st) instar larvae Ae.aegypti was introduced into each bucket weekly without additional TMOF-Bti rice husk formulation or wettable powder.The experiment was repeated for four times.Results:The result of the study showed that all formulations were very effective on the first two weeks by giving 100% larval mortality for all concentrations applied.The TMOF(2%) + Bti(2%) had a good residual effect until the end of 3^(rd) week,TMOF(4%) + Bti(4%) until 4^(th) week,wettable powder TMOF(20%) + Bti(20%) until the third week.Condusions:From the results it can be concluded that the TMOF-Bti formulations can be utilized in dengue vector control.
文摘The current experiment was conducted to find out the optimal conditions for mass rearing and developmental changes of Cyclosainsulana. The lab. conditions were maintained at （27±2）℃ and （65±5）% RH. The clear perplex cages and natural diet consisting of the aphids, larvae of the house fly and larvae of drosophila were used for rearing. C. insulana took （123.12±7.26） days to develop from eggs to adults passing through eight instars under prevailing vivo conditions. The eggs were greenish white in color with average size of 0.57 mm ±0.034. The eggs spent （7.52±1.64） days in emergence. Maximum number of spiderlings survived at the 5th instar （84%） and minimum at the 1st instar （34%）. The measurements of different body parts including the total body length, cephalothorax and pedipalps of the both male and female C. insulana were done with the help of micrometer and presented as mean± SD. The measurements varied in the each developing instar. It was concluded that spiders were difficult to rear in the lab. conditions and each developing stage which was regarded as instars showed variations in size colors and body characteristics.
基金Supported by Innovation Project of Graduate Education of Jiangsu Province(CXZZ11_0538)Project of Nanjing Construction Committee and the Administration Bureau of Dr.Sun Yat-sen's Mausoleum(200409)
文摘Apriona germari larvae were obtained via sample collection in woodland and indoor rearing. In this study, larval instars were divided by Dyar＇s law, and the results showed that the larval stage of A. germari comprised six instars, while the width of head capsule was the best instar-dividing structure. On the basis of instar division, tubercle numbers on the transverse furrow downside of first abdominal dorsal ambulatory ampullae, seta numbers of abdominal telson and lateral seta numbers of mandible were observed and statistically calculated. The accuracy of instar-dividing was verified by comparing with the Dyar＇s law. The coincidence rate of two instar-dividing results was 90. 47%. It is approved that the above morphological features could be used in instar division ofA. germari larvae.
基金Supported by the Science and Technology Foundation of Fujian Province (No. 2004N002)the National Natural Science Foundation of China (No. 30570408)
文摘Polyphenol oxidase （PPO） is the enzyme responsible for enzymatic browning during the growth of insects. It is also involved in defense reactions and is related with immunities in insects. PPO a metalloenzyme oxidase, catalyzes the oxidation of o-diphenol to o-quinone. The present paper describes the effects of benzaldehyde and its p-substituted derivatives on the activity of PPO from the fifth instar of Pieris rapae L. PPO from the fifth instar of Pieris rapae L. was purified using ammonium sulfate fractionation and chromatography on Sephadex G-100. The enzyme kinetics was characterized using L-3,4-dihydroxyphenylalanine （L-DOPA） as substrate. The results show that benzaldehyde, phydroxybenzaldehyde, p-chlorobenzaldehyde, and p-cyanobenzaldehyde can inhibit the PPO activity for the oxidation of L-DOPA. The inhibitor concentration leading to 50% activity lost, IC50, was estimated to be 5.90, 5.62, 2.83, and 2.91 mmol/L for the four tested inhibitors, respectively. Kinetic analyses show that the inhibitory effects of these compounds are reversible. Benzaldehyde, phydroxybenzaldehyde, and p-chlorobenzaldehyde are noncompetitive inhibitors while p-cyanobenzaldehyde is a mixed-type inhibitor. The inhibition constants were determined for all four inhibitors. p-chlorobenzaldehyde and p-cyanobenzaldehyde were more potent inhibitors than the other compounds. These results provide a basis for developing PPO inhibition-based pesticides.
