AIM：To detect the impact of insulin-like growth factor-1（IGF-1）and other risk factors for the early prediction of retinopathy of prematurity（ROP）and to establish a scoring system for ROP prediction by using clini...AIM：To detect the impact of insulin-like growth factor-1（IGF-1）and other risk factors for the early prediction of retinopathy of prematurity（ROP）and to establish a scoring system for ROP prediction by using clinical criteria and serum IGF-1 levels.METHODS：The study was conducted with 127 preterm infants.IGF-1 levels in the 1st day of life,1st,2nd,3rd and4th week of life was analyzed.The score was established after logistic regression analysis,considering the impact of each variable on the occurrences of any stage ROP.A validation cohort containing 107 preterm infants was included in the study and the predictive ability of ROP score was calculated.RESULTS：Birth weights（BW）,gestational weeks（GW）and the prevalence of breast milk consumption were lower,respiratory distress syndrome（RDS）,bronchopulmonarydysplasia（BPD）and necrotizing enterocolitis（NEC）were more frequent,the duration of mechanical ventilation and oxygen supplementation was longer in patients with ROP（P〈0.05）.Initial serum IGF-1 levels tended to be lower in newborns who developed ROP.Logistic regression analysis revealed that low BW（〈1250 g）,presence of intraventricular hemorrhage（IVH）and formula feeding increased the risk of ROP.Afterwards,the scoring system was validated on 107 infants.The negative predictive values of a score less than 4 were 84.3%,74.7%and 79.8%while positive predictive values were 76.3%,65.5%and71.6%respectively.CONCLUSION：In addition to BW〈1250 g and IVH,formula consumption was detected as a risk factor for the development of ROP.Breastfeeding is important for prevention of ROP in preterm infants.展开更多
本研究旨在探讨不同蛋白质水平日粮对绵羊胰岛素样生长因子-1(IGF-1)和生长激素(GH)分泌及基因mRNA表达量的影响,为科学配置肉羊饲料及研究肉羊生长发育提供基础。选择6月龄体重相近的多胎萨福克公羔18只,随机分为3组,分别饲喂不同蛋白...本研究旨在探讨不同蛋白质水平日粮对绵羊胰岛素样生长因子-1(IGF-1)和生长激素(GH)分泌及基因mRNA表达量的影响,为科学配置肉羊饲料及研究肉羊生长发育提供基础。选择6月龄体重相近的多胎萨福克公羔18只,随机分为3组,分别饲喂不同蛋白质水平的日粮(低蛋白日粮、中蛋白日粮和高蛋白日粮)。采用ELISA方法和SYBR Green Real-time PCR方法检测日粮不同蛋白质水平对不同生长发育阶段(30、60、90和120d)羔羊外周血中IGF-1、GH浓度和皮肤组织中基因表达的影响。结果显示,日粮蛋白质水平显著影响绵羊平均日增重、外周血中IGF-1和GH的浓度以及皮肤组织IGF-1基因的表达丰度,而未显著影响GH基因的表达丰度。结果提示,随着日粮蛋白质水平的升高,绵羊生长发育快,外周血中IGF-1浓度增加,GH浓度降低,IGF-1基因表达量增加。展开更多
目的研究补肾壮骨颗粒是否通过提高快速老化小鼠(P6)局部骨组织GH/IGF-1轴的基因表达水平从而提高骨密度。方法实验分4组:R1小鼠生理盐水灌胃组(R1组),P6小鼠分为生理盐水灌胃组(P6空组)、皮下rh GH组(rh GH组)和补肾壮骨颗粒灌胃组(补...目的研究补肾壮骨颗粒是否通过提高快速老化小鼠(P6)局部骨组织GH/IGF-1轴的基因表达水平从而提高骨密度。方法实验分4组:R1小鼠生理盐水灌胃组(R1组),P6小鼠分为生理盐水灌胃组(P6空组)、皮下rh GH组(rh GH组)和补肾壮骨颗粒灌胃组(补肾组),每组各10只,每日干预1次。分别干预3、6个月后进行骨密度测量和胫骨GH m RNA及IGF-1 m RNA表达水平检测。结果干预3个月后,骨密度比较:R1组及补肾组高于P6空组;rh GH组与P6空组比较差异无统计学意义。GH m RNA和IGF-1 m RNA表达水平比较:R1组、rh GH组及补肾组均高于P6空组。干预6个月后,骨密度比较:rh GH组及补肾组较P6空组提高。GH m RNA和IGF-1 m RNA表达水平比较:GH组及补肾组较P6空组均有所上升。4组GH m RNA表达水平与IGF-1 m RNA表达水平呈正相关。P6各组GH m RNA、IGF-1 m RNA表达水平与全身各部位骨密度呈正相关。结论补肾壮骨颗粒可以提高P6小鼠全身各部位的骨密度,其作用机制可能与提高局部骨组织GH m RNA与IGF-1 m RNA表达水平有关。展开更多
文摘AIM：To detect the impact of insulin-like growth factor-1（IGF-1）and other risk factors for the early prediction of retinopathy of prematurity（ROP）and to establish a scoring system for ROP prediction by using clinical criteria and serum IGF-1 levels.METHODS：The study was conducted with 127 preterm infants.IGF-1 levels in the 1st day of life,1st,2nd,3rd and4th week of life was analyzed.The score was established after logistic regression analysis,considering the impact of each variable on the occurrences of any stage ROP.A validation cohort containing 107 preterm infants was included in the study and the predictive ability of ROP score was calculated.RESULTS：Birth weights（BW）,gestational weeks（GW）and the prevalence of breast milk consumption were lower,respiratory distress syndrome（RDS）,bronchopulmonarydysplasia（BPD）and necrotizing enterocolitis（NEC）were more frequent,the duration of mechanical ventilation and oxygen supplementation was longer in patients with ROP（P〈0.05）.Initial serum IGF-1 levels tended to be lower in newborns who developed ROP.Logistic regression analysis revealed that low BW（〈1250 g）,presence of intraventricular hemorrhage（IVH）and formula feeding increased the risk of ROP.Afterwards,the scoring system was validated on 107 infants.The negative predictive values of a score less than 4 were 84.3%,74.7%and 79.8%while positive predictive values were 76.3%,65.5%and71.6%respectively.CONCLUSION：In addition to BW〈1250 g and IVH,formula consumption was detected as a risk factor for the development of ROP.Breastfeeding is important for prevention of ROP in preterm infants.
文摘本研究旨在探讨不同蛋白质水平日粮对绵羊胰岛素样生长因子-1(IGF-1)和生长激素(GH)分泌及基因mRNA表达量的影响,为科学配置肉羊饲料及研究肉羊生长发育提供基础。选择6月龄体重相近的多胎萨福克公羔18只,随机分为3组,分别饲喂不同蛋白质水平的日粮(低蛋白日粮、中蛋白日粮和高蛋白日粮)。采用ELISA方法和SYBR Green Real-time PCR方法检测日粮不同蛋白质水平对不同生长发育阶段(30、60、90和120d)羔羊外周血中IGF-1、GH浓度和皮肤组织中基因表达的影响。结果显示,日粮蛋白质水平显著影响绵羊平均日增重、外周血中IGF-1和GH的浓度以及皮肤组织IGF-1基因的表达丰度,而未显著影响GH基因的表达丰度。结果提示,随着日粮蛋白质水平的升高,绵羊生长发育快,外周血中IGF-1浓度增加,GH浓度降低,IGF-1基因表达量增加。
文摘目的研究补肾壮骨颗粒是否通过提高快速老化小鼠(P6)局部骨组织GH/IGF-1轴的基因表达水平从而提高骨密度。方法实验分4组:R1小鼠生理盐水灌胃组(R1组),P6小鼠分为生理盐水灌胃组(P6空组)、皮下rh GH组(rh GH组)和补肾壮骨颗粒灌胃组(补肾组),每组各10只,每日干预1次。分别干预3、6个月后进行骨密度测量和胫骨GH m RNA及IGF-1 m RNA表达水平检测。结果干预3个月后,骨密度比较:R1组及补肾组高于P6空组;rh GH组与P6空组比较差异无统计学意义。GH m RNA和IGF-1 m RNA表达水平比较:R1组、rh GH组及补肾组均高于P6空组。干预6个月后,骨密度比较:rh GH组及补肾组较P6空组提高。GH m RNA和IGF-1 m RNA表达水平比较:GH组及补肾组较P6空组均有所上升。4组GH m RNA表达水平与IGF-1 m RNA表达水平呈正相关。P6各组GH m RNA、IGF-1 m RNA表达水平与全身各部位骨密度呈正相关。结论补肾壮骨颗粒可以提高P6小鼠全身各部位的骨密度,其作用机制可能与提高局部骨组织GH m RNA与IGF-1 m RNA表达水平有关。
