1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to sear...1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to search for a long-acting insulin preparation from the beginning of medial me in practice. After the explorations for a long time, a series of prolonged-acting insulin preparations have been used in the treatment of diabetes. But most of them展开更多
In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Z...In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.展开更多
基金Project supported by grants of UNIDO (91/048) and "863" High-Fech Program.
文摘1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to search for a long-acting insulin preparation from the beginning of medial me in practice. After the explorations for a long time, a series of prolonged-acting insulin preparations have been used in the treatment of diabetes. But most of them
文摘In the presence of a series of small organic molecules including 1, 4 dioxane, DMF, isopropanol, rDNA human insulin, mutants A21 Asn→Ser and (A21 Asn→Ser, B27 Thr→Arg) human insulin have been crystallized in both Zn 2+ containing and Zn 2+ free system. Preliminary crystallographic analyses show that they all belong to T 3R 3 structural type. The results indicate that, except phenolic derivatives and lyotropic anions reported before, some other appropriate organic molecules can also induce the T 3R 3 conformation transition of insulin.
