Integrated Expression of the Oenococcus oeni mleA Gene in Saccharomyces cerevisiae

Malolactic enzyme is the function enzyme which catalyses the reaction for L-malate converting to L-lactic during malolactic fermentation （MLF）. In this paper, researches concerning the malolactic enzyme gene mleA cloned from a patent strain Oenococcus oeni SD-2a screened in Chinese wine and integrated expressing in Saccharomyces cerevisiae were performed in order to carry out both alcoholic fermentation （AF） and malolactic fermentation （MLF） during winemaking, with a view to achieving a better control of MLF in enology. To construct the expression plasmid named pYILmleA, cloned mleA gene, PGK1 promoter, and ADH1 terminator were ligated and inserted into integrating vector YIp5. Yeast transformants were screened on SD/-Ura and identified by auxotrophic test, mating type test, and colony PCR. Target protein was detected by SDS-PAGE and the targeted gene integrated to the chromosome was detected by dot bloting hybridization. After the transformant was cultured in SD/-Ura adding glucose （10%） and L-malate （5 648 mg L-1） for 4 d, the culture supernatant was collected and L-malate and L-lactic acid contents were detected by HPLC. 1 278-1 312 mg L-1 L-lactic acids were detected, while the comparative drop rates of L-malate were 20.18-20.85%. L-malate and L-lactic contents of the transformants showed extra significant difference and significant difference with the control ones by t-test respectively. The result indicated that the functional expression was achieved in recombinants S. cerevisiae.

Functional design of wind turbine airfoils based on roughness sensitivity characteristics

To improve aerodynamic performance of wind turbine airfoils,the shape profile characteristic of the airfoil is investigated.Application of conformal transformation,one functional and integrated expression of wind turbine airfoils is presented.Using the boundary layer theory,the aerodynamic model with roughness of wind turbine airfoils is introduced by studying flow separation around the airfoil.Based on the shape expression and aerodynamic performance of airfoils,the function design of wind turbine airfoils is carried out that the maximum lift-drag ratio and low roughness sensitivity are designed objects.Three wind turbines airfoils with different thickness are gained which are used at tip part of blades.As an example,the aerodynamic performance of one designed airfoil with relative thickness of 15%is simulated in different conditions of clean surface,rough surface,laminar flow and turbulent flow.The comparison of aerodynamic performance between the designed airfoil and one popular NACA airfoil is completed which can verify the better performance of the designed airfoil and reliability of the designed method.

Determination of the chromosomal position effects for plug-and-play application in the Myxococcus xanthus chassis cells

The chromosomal position effect can significantly affect the transgene expression,which may provide an efficient strategy for the inauguration of alien genes in new hosts,but has been less explored rationally.The bacterium Myxococcus xanthus harbors a large circular high-GC genome,and the position effect in this chassis may result in a thousand-fold expression variation of alien natural products.In this study,we conducted transposon insertion at TA sites on the M.xanthus genome,and used enrichment and dilution indexes to respectively appraise high and low expression potentials of alien genes at insertion sites.The enrichment sites are characteristically distributed along the genome,and the dilution sites are overlapped well with the horizontal transfer genes.We experimentally demonstrated the enrichment sites as high expression integration sites(HEISs),and the dilution sites unsuitable for gene integration expression.This work highlights that HEISs are the plug-and-play sites for efficient expression of integrated genes.

Matching suitable feature construction for SAR images based on evolutionary synthesis strategy

In the paper,a set of algorithms to construct synthetic aperture radar（SAR）matching suitable features are frstly proposed based on the evolutionary synthesis strategy.During the process,on the one hand,the indexes of primary matching suitable features（PMSFs）are designed based on the characteristics of image texture,SAR imaging and SAR matching algorithm,which is a process involving expertise;on the other hand,by designing a synthesized operation expression tree based on PMSFs,a much more flexible expression form of synthesized features is built,which greatly expands the construction space.Then,the genetic algorithm-based optimized searching process is employed to search the synthesized matching suitable feature（SMSF）with the highest effciency,largely improving the optimized searching effciency.In addition,the experimental results of the airborne synthetic aperture radar ortho-images of C-band and P-band show that the SMSFs gained via the algorithms can reflect the matching suitability of SAR images accurately and the matching probabilities of selected matching suitable areas of ortho-images could reach 99±0.5%.