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Key issues and progress of industrial big data-based intelligent blast furnace ironmaking technology 被引量:1
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作者 Quan Shi Jue Tang Mansheng Chu 《International Journal of Minerals,Metallurgy and Materials》 SCIE EI CAS CSCD 2023年第9期1651-1666,共16页
Blast furnace (BF) ironmaking is the most typical “black box” process, and its complexity and uncertainty bring forth great challenges for furnace condition judgment and BF operation. Rich data resources for BF iron... Blast furnace (BF) ironmaking is the most typical “black box” process, and its complexity and uncertainty bring forth great challenges for furnace condition judgment and BF operation. Rich data resources for BF ironmaking are available, and the rapid development of data science and intelligent technology will provide an effective means to solve the uncertainty problem in the BF ironmaking process. This work focused on the application of artificial intelligence technology in BF ironmaking. The current intelligent BF ironmaking technology was summarized and analyzed from five aspects. These aspects include BF data management, the analyses of time delay and correlation, the prediction of BF key variables, the evaluation of BF status, and the multi-objective intelligent optimization of BF operations. Solutions and suggestions were offered for the problems in the current progress, and some outlooks for future prospects and technological breakthroughs were added. To effectively improve the BF data quality, we comprehensively considered the data problems and the characteristics of algorithms and selected the data processing method scientifically. For analyzing important BF characteristics, the effect of the delay was eliminated to ensure an accurate logical relationship between the BF parameters and economic indicators. As for BF parameter prediction and BF status evaluation,a BF intelligence model that integrates data information and process mechanism was built to effectively achieve the accurate prediction of BF key indexes and the scientific evaluation of BF status. During the optimization of BF parameters, low risk, low cost, and high return were used as the optimization criteria, and while pursuing the optimization effect, the feasibility and site operation cost were considered comprehensively.This work will help increase the process operator’s overall awareness and understanding of intelligent BF technology. Additionally, combining big data technology with the process will improve the practicality of data models in actual production and promote the application of intelligent technology in BF ironmaking. 展开更多
关键词 BF ironmaking intelligent BF industrial big data machine learning integrated mechanism and data
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Study on a Bowl-based Mechanism for Transplanting Potted Strawberry Seedlings
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作者 Yin Da-qing Yang Yu-chao +2 位作者 Zhou Mai-le Wei Ming-xu Wang Jin-wu 《Journal of Northeast Agricultural University(English Edition)》 CAS 2023年第1期56-68,共13页
To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to ac... To improve the efficiency of fetching and transplanting seedlings for the mechanization of strawberry planting,an integrated transplanting mechanism was designed with protruding,fetching and planting performance to achieve rapid fetching and pushing bowl movements.According to the working principle of the slewing mechanism,a kinematics model and the optimization goal were established,respectively.Based on visual auxiliary analysis software,optimal parameters were obtained.A three-dimensional model was established to obtain a simulation trajectory by means of a virtual simulation design analysis.Three-dimensional printing technology was used to manufacture the test prototype,and the actual working trajectories of the test prototype were extracted using high-speed photography technology,which verified the consistency of the actual trajectory with the theoretical and simulated trajectories.A prototype transplanting experiment was performed with the success rate of seedling extraction of 91.2%and excellent planting rate of 82.8%,which met the requirements for integrated strawberry harvesting,planting and transplanting.The experimental results verified the correctness and feasibility of the design of integrated transplanting mechanism. 展开更多
关键词 potted strawberry seedlings potted seedling transplanting integrated planting mechanism protruding and pushing bowl type parameter optimization
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Corrosion behavior of Mg-Zn-Zr-RE alloys under physiological environment-Impact on mechanical integrity and biocompatibility 被引量:2
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作者 Marwa AbdelGawad Chaudhry A.Usman +2 位作者 Vasanth C.Shunmugasamy Ibrahim Karaman Bilal Mansoor 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2022年第6期1542-1572,共31页
Benefits of RE addition on Mg alloys strength and corrosion resistance are widely reported but their effects on biodegradability and biocompatibility are still of concern.This paper investigates the effect of RE addit... Benefits of RE addition on Mg alloys strength and corrosion resistance are widely reported but their effects on biodegradability and biocompatibility are still of concern.This paper investigates the effect of RE additions on biodegradability of Mg-Zn alloys under simulated physiological conditions.In this context,two commercial Mg-Zn-Zr-RE alloys,namely ZE41 and EZ33,with same RE addition but different concentrations are studied in Hank’s Balanced Salt Solution(HBSS)at 37℃and with pH of 7.4.Weight-loss,hydrogen evolution,real-time insitu drop test,electrochemical impedance spectroscopy(EIS)and potentiodynamic polarization are deployed to study corrosion characteristics.The mechanical integrity of both alloys is assessed by mechanical testing post immersion.Furthermore,in vitro biocompatibility is evaluated by indirect cytotoxicity tests using NIH3T3 cells.Results reveal that although both alloys showed similar microstructure,size and distribution of precipitates played a significant role on its corrosion response.EIS and open circuit potential results show stable film formation on EZ33,while ZE41 showed passive layer formation followed by its deterioration,over the analyzed time period.Using real-time drop test,it was shown in ZE41 alloy that both T-phase and Zr-rich precipitates acted as micro cathodes,resulting in an unstable surface film.In EZ33,Zr-rich regions did not influence corrosion response,resulting in better corrosion resistance that was corroborated by post-immersion surface morphology investigations.The higher degradation observed in ZE41 alloy resulted in higher drop in flexural and tensile strength compared to EZ33 alloy.In addition,cytotoxicity tests on NIH3T3 cells revealed that cell viability of EZ33 increased with increasing incubation time,contrary to ZE41,owing to its lower biodegradation behavior and despite higher concentrations of REs.Present results show that an increase in RE concentration in EZ33,relative to ZE41,had a positive effect on corrosion rate that subsequently controlled alloy mechanical integrity and biocompatibility. 展开更多
关键词 Magnesium alloy Corrosion EIS Mechanical integrity CYTOTOXICITY
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Electromagnetic and Mechanical Stress Analysis of Wind-Driven Synchronous Reluctance Generator 被引量:1
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作者 Kitaba Tefera Praveen Tripathy Senior Ravindranath Adda 《CES Transactions on Electrical Machines and Systems》 CSCD 2019年第1期107-114,共8页
The investigation explores the mechanical stress and electromagnetic performance for a wind-driven synchronous reluctance generator(SRG).The change in the mechanical stress due to the presence of centripetal force,win... The investigation explores the mechanical stress and electromagnetic performance for a wind-driven synchronous reluctance generator(SRG).The change in the mechanical stress due to the presence of centripetal force,wind speed,and rotor speed are evaluated for different thickness of tangential and radial ribs.Moreover,the variation in the electromagnetic feature such as the q−and d−axes flux,reactance ratio,inductance,torque and torque ripple are discussed for different thickness of tangential and radial ribs.Increasing both tangential and radial ribs thickness has an effect on the electromagnetic performance,but it is observed that effect is significantly more with the variation of tangential rib thickness.Similarly,the mechanical stress analysis for rotor design has been explored in this paper.It is observed that high concentration of peak stress on the rotor ribs,which limits the range of rotor speed. 展开更多
关键词 ELECTROMAGNETIC mechanical integrity synchronous reluctance generator stress and safety factor wind speed.
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Application of canonical coordinates for solving single-freedom constraint mechanical systems 被引量:1
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作者 高芳 张晓波 傅景礼 《Applied Mathematics and Mechanics(English Edition)》 SCIE EI 2014年第8期1029-1038,共10页
This paper introduces the canonical coordinates method to obtain the first integral of a single-degree freedom constraint mechanical system that contains conserva-tive and non-conservative constraint homonomic systems... This paper introduces the canonical coordinates method to obtain the first integral of a single-degree freedom constraint mechanical system that contains conserva-tive and non-conservative constraint homonomic systems. The definition and properties of canonical coordinates are introduced. The relation between Lie point symmetries and the canonical coordinates of the constraint mechanical system are expressed. By this re-lation, the canonical coordinates can be obtained. Properties of the canonical coordinates and the Lie symmetry theory are used to seek the first integrals of constraint mechanical system. Three examples are used to show applications of the results. 展开更多
关键词 canonical coordinates first integral Lie symmetry constraint mechanical system
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Osteocyte pericellular and perilacunar matrices as markers of bone-implant mechanical integrity
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作者 ReMY GAUTHIER HeLÈNE FOLLET +5 位作者 ANA-MARIA TRUNFIO-SFARGHIU DELPHINE FARLAY NINA ATTIK SYLVAIN MEILLE JeRÔME CHEVALIER DAVID MITTON 《BIOCELL》 SCIE 2022年第10期2209-2216,共8页
To develop durable bone healing strategies through improved control of bone repair,it is of critical importance to understand the mechanisms of bone mechanical integrity when in contact with biomaterials and implants.... To develop durable bone healing strategies through improved control of bone repair,it is of critical importance to understand the mechanisms of bone mechanical integrity when in contact with biomaterials and implants.Bone mechanical integrity is defined here as the adaptation of structural properties of remodeled bone in regard to an applied mechanical loading.Accordingly,the authors present why future investigations in bone repair and regeneration should emphasize on the matrix surrounding the osteocytes.Osteocytes are mechanosensitive cells considered as the orchestrators of bone remodeling,which is the biological process involved in bone homeostasis.These bone cells are trapped in an interconnected porous network,the lacunocanalicular network,which is embedded in a bone mineralized extracellular matrix.As a consequence of an applied mechanical loading,the bone deformation results in the deformation of this lacunocanalicular network inducing a shift in interstitial fluid pressure and velocity,thus resulting in osteocyte stimulation.The material environment surrounding each osteocyte,the so called perilacunar and pericellular matrices properties,define its mechanosensitivity.While this mechanical stimulation pathway is well known,the laws used to predict bone remodeling are based on strains developing at a tissue scale,suggesting that these strains are related to the shift in fluid pressure and velocity at the lacunocanalicular scale.While this relationship has been validated through observation in healthy bone,the fluid behavior at the bone-implant interface is more complex.The presence of the implant modifies fluid behavior,so that for the same strain at a tissue scale,the shift in fluid pressure and velocity will be different than in a healthy bone tissue.In that context,new markers for bone mechanical integrity,considering fluid behavior,have to be defined.The viewpoint exposed by the authors indicates that the properties of the pericellular and the perilacunar matrices have to be systematically investigated and used as structural markers of fluid behavior in the course of bone biomaterial development. 展开更多
关键词 Bone mechanical integrity OSTEOCYTES Pericellular Perilacunar MECHANOSENSITIVITY
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Evaluation of corrosion resistance, mechanical integrity loss and biocompatibility of PCL/HA/TiO_(2) hybrid coated biodegradable ZM21 Mg alloy
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作者 Navdeep Singh Uma Batra +1 位作者 Kamal Kumar Anil Mahapatro 《Journal of Magnesium and Alloys》 SCIE EI CAS CSCD 2022年第11期3179-3204,共26页
A novel PCL/HA/TiO_(2)hybrid coating on ZM21 Mg alloy substrate has been investigated for corrosion resistance, biocompatibility and mechanical integrity loss in terms of bending, compressive and tensile strength in p... A novel PCL/HA/TiO_(2)hybrid coating on ZM21 Mg alloy substrate has been investigated for corrosion resistance, biocompatibility and mechanical integrity loss in terms of bending, compressive and tensile strength in physiological media. The prepared hybrid coating was dip coated over ZM21 from HA/TiO_(2)and PCL solutions followed by creating a microporous PCL layer by utilizing Non-solvent Induced Phase Separation(NIPS) technique. The electrochemical measurement and in-vitro degradation study in SBF after 28 days showed that the PCL/HA/TiO_(2) hybrid coating reduced H2 evolution rate, weight loss, and corrosion rate by 64, 116 and 118 times respectively, as compared to uncoated ZM21 samples. The surface studies carried out using SEM-EDX, FTIR and XRD revealed formation of highly stable 3d flower-like HA crystals with Ca/P ratio of 1.60 in the PCL micropores. This dense apatite growth effectively protected the PCL/HA/TiO_(2)hybrid coated samples to maintain the good mechanical integrity even after 28 days of immersion as compared to HA/TiO_(2)composite coated, As-polished(A/P) and As-machined(A/M) samples. The failure analysis of samples under mechanical loading were performed using SEM-BSE-EBSD.The in-vitro cellular viability of L929 fibroblast cells on PCL/HA/TiO_(2)hybrid coating was found 50.47% higher with respect to control group,whereas bacterial viability was supressed by 57.15 and 62.35% against gram-positive Staphylococcus aureus and gram-negative Escherichia coli bacterial models. The comprehensive assessment indicates PCL/HA/TiO_(2)hybrid coating as a suitable candidate to delay early degradation and mechanical integrity loss of Mg-based alloys for devising biodegradable orthopaedic implant. 展开更多
关键词 Magnesium alloy Surface modification Hybrid coating Mechanical integrity loss Degradation rate BIOCOMPATIBILITY
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Mechanical Integrity and Failure Analysis of Photovoltaic Modules under Simulated Snow Loads Using Pneumatic Airbag Setup
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作者 Nouman Ali Shah Rizwan M. Gul Zafar Hayat Khan 《Journal of Power and Energy Engineering》 2022年第1期1-13,共13页
Photovoltaic (PV) modules have emerged as an ideal technology of choice for <span>harvesting vastly available renewable energy resources. However, the effi</span>ciency <span>of PV modules remains si... Photovoltaic (PV) modules have emerged as an ideal technology of choice for <span>harvesting vastly available renewable energy resources. However, the effi</span>ciency <span>of PV modules remains significantly lower than that of other renewable</span> energy sources such as wind and hydro. One of the critical elements affecting a photovoltaic module’s efficiency is the variety of external climatic conditions under which it is installed. In this work, the effect of simulated snow loads was evaluated on the performance of PV modules with different <span>types of cells and numbers of busbars. According to ASTM-1830 and IEC-1215</span> standards, a load of 5400 Pa was applied to the surface of PV modules for 3 hours. An indigenously developed pneumatic airbag test setup was used for the uniform application of this load throughout the test, which was validated by load cell and pressure gauge. Electroluminescence (EL) imaging and solar flash tests were performed before and after the application of load to characterize the performance and effect of load on PV modules. Based on these tests, the maxi<span>mum power output, efficiency, fill factor and series resistance were deter</span>mined. The results show that polycrystalline modules are the most likely to withstand the snow loads as compared to monocrystalline PV modules. A maximum drop of 32.13% in the power output and a 17.6% increase in series resistance were observed in the modules having more cracks. These findings demonstrated the efficacy of the newly established test setup and the potential of snow loads for reducing the overall performance of PV module. 展开更多
关键词 Photovoltaic Modules Pneumatic Testing Setup Mechanical Integrity Electroluminescence Testing Electrical Performance
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Approach and practice: integrating earth observation resources for data sharing in China GEOSS
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作者 Lianchong Zhang Guoqing Li +2 位作者 Chi Zhang Huanyin Yue Xiaohan Liao 《International Journal of Digital Earth》 SCIE EI 2019年第12期1441-1456,共16页
Earth observation data sharing is an essential part of the data lifecycle and plays a critical role in Earth science research.Existing industry data sharing systems are affected by restrictions in distributed resource... Earth observation data sharing is an essential part of the data lifecycle and plays a critical role in Earth science research.Existing industry data sharing systems are affected by restrictions in distributed resource management and tightly coupled service interoperability.These systems currently offer no support for facilitating cross-disciplinary exploration and application.The lack of a national data sharing infrastructure has led to reduced international cooperation.These barriers are common and have hindered the development of the Global Earth Observation System of Systems(GEOSS).The China GEOSS Data Sharing Network(China GEOSS DSNet)has been proposed as a part of China’s Plan for Implementing GEOSS(2016–2025)to address the above issues.In this research,we designed a national GEOSS data sharing framework,including resource integration mechanism,sharing-oriented metadata standards,and lightweight interoperability service to coordinate various Earth observation resources.So far,more than 29 million archived satellite metadata records and 200 TB of high-quality satellite datasets have been integrated under this framework.The results were demonstrated in the following applications:domestic satellite archived metadata query service,international Earth observation resource sharing service,and disaster emergency response service. 展开更多
关键词 Earth observation data sharing China GEOSS integration mechanism
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A review of the mechanical integrity and electrochemical performance of flexible lithium-ion batteries
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作者 Ahmad Shokrieh Amir Hossein Mirzaei +2 位作者 Lijuan Mao Mahmood M.Shokrieh Zhixiang Wei 《Nano Research》 SCIE EI CSCD 2023年第12期12962-12982,共21页
In the past two decades,various research works have been conducted in the field of flexible electronic devices(FEDs).Researchers have focused their efforts on solving the existing challenges in the electronic,electroc... In the past two decades,various research works have been conducted in the field of flexible electronic devices(FEDs).Researchers have focused their efforts on solving the existing challenges in the electronic,electrochemical,and mechanical behaviors of FEDs.The importance of flexible lithium-ion batteries(FLIBs)in the area of FEDs is evident;however,less attention has been paid to the mechanical behavior of FLIBs in comparison with the material and electrochemical characteristics.The present paper reviewed the research works in the FLIBs,focusing on their mechanical integrity and electrochemical performances.First,an introduction to FLIBs was presented,and the previous review papers published in this field were briefly introduced.Then,a detailed review of the available electrochemical and mechanical research works on FLIBs was presented.Moreover,the mechanical testing methods(tensile,compressive,indentation,fatigue,and adhesion)for the characterization of FLIBs’components,the research works on the simulation and modeling of the mechanical behavior of FLIBs,and a summary of the present situation and the future trend of research in this field were reviewed and presented. 展开更多
关键词 flexible devices electrochemical performance mechanical integrity testing methods simulation and modeling.
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Predicting localised corrosion and mechanical performance of a PEO surface modified rare earth magnesium alloy for implant use through in-silico modelling
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作者 Kerstin van Gaalen Conall Quinn +5 位作者 Marek Weiler Felix Gremse Felix Benn Peter E.McHugh Ted J.Vaughan Alexander Kopp 《Bioactive Materials》 SCIE CSCD 2023年第8期437-451,共15页
In this study,the influence of a plasma electrolytic oxidation(PEO)surface treatment on a medical-grade WE43-based magnesium alloy is examined through an experimental and computational framework that considers the eff... In this study,the influence of a plasma electrolytic oxidation(PEO)surface treatment on a medical-grade WE43-based magnesium alloy is examined through an experimental and computational framework that considers the effects of localised corrosion features and mechanical properties throughout the corrosion process.First,a comprehensive in-vitro immersion study was performed on WE43-based tensile specimens with and without PEO surface modification,which included fully automated spatial reconstruction of the phenomenological features of corrosion through micro-CT scanning,followed by uniaxial tensile testing.Then the experimental data of both unmodified and PEO-modified groups were used to calibrate parameters of a finite element-based surface corrosion model.In-vitro,it was found that the WE43-PEO modified group had a significantly lower corrosion rate and maintained significantly higher mechanical properties than the unmodified.While corrosion rates were~50%lower in the WE43-PEO modified specimens,the local geometric features of corroding surfaces remained similar to the unmodified WE43 group,however evolving after almost the double amount of time.We were also able to quantitatively demonstrate that the PEO surface treatment on magnesium continued to protect samples from corrosion throughout the entire period tested,and not just in the early stages of corrosion.Using the results from the testing framework,the model parameters of the surface-based corrosion model were identified for both groups.This enabled,for the first time,in-silico prediction of the physical features of corrosion and the mechanical performance of both unmodified and PEO modified magnesium specimens.This simulation framework can enable future in-silico design and optimisation of bioabsorbable magnesium devices for load-bearing medical applications. 展开更多
关键词 Plasma electrolytic oxidation WE43MEO Degradation model Corrosion phenomenology Mechanical integrity
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An overview of the environmental finance policies in China: retrofitting an integrated mechanism for environmental management 被引量:26
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作者 Wei LI Mengze HU 《Frontiers of Environmental Science & Engineering》 SCIE EI CAS CSCD 2014年第3期316-328,共13页
Considering the significant roles of the policies in developing environmental finance, an overview is conducted on the environmental finance policies (EFPs) in China. This paper analyzed the definition, scope, evolu... Considering the significant roles of the policies in developing environmental finance, an overview is conducted on the environmental finance policies (EFPs) in China. This paper analyzed the definition, scope, evolution and main instruments of EFPs. The implementation progress of financial activities on each instrument are investigated respectively. Then the experiences learned from and failures discovered in the development of the EFPs are discussed well recommendations for further improvement of the EFPs and their implementation are provided. Our study found that the EFPs have been established in China after a four-phase evolution since the early 1980s. The policies have played a critical role in leading to a rapid development in environmental finance by involving more financial instruments to accomplish the objective-led environmental plans. Driven by the policies, the new green credit (GC), green security (GS), and green insurance (GI) instruments have been phased in as supplements to the conventional command and control approaches to improve the environmental governance of financial activities and pollution sources. However, the market mechanism of financial institution is limited due to their defensive and incapable performance on implementation some of EFP instruments. To further strengthen the effectiveness of EFPs in facilitating environmental man- agement, recommendations are made mainly on the aspects including developing more specific policy guidelines, enhancing information sharing and disclosure, providing sufficient economic incentives, establishing environmental liabilities with financial activities, and involving issues related to climate change, and biodiversity and ecosystem service. 展开更多
关键词 environmental finance policy integrated mechanism phased evolution implementation progress financial institution environmental management
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Integration mechanisms of transgenes and population fitness of GH transgenic fish 被引量:27
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作者 HU Wei &ZHU ZuoYan 《Science China(Life Sciences)》 SCIE CAS 2010年第4期401-408,共8页
It has been more than 20 years since the first batch of transgenic fish was produced. Five stable germ-line transmitted growth hormone (GH) transgenic fish lines have been generated. This paper reviews the mechanisms ... It has been more than 20 years since the first batch of transgenic fish was produced. Five stable germ-line transmitted growth hormone (GH) transgenic fish lines have been generated. This paper reviews the mechanisms of integration and gene targeting of the transgene, as well as the viability, reproduction and transgenic approaches for the reproductive containment of GH-transgenic fish. Further, we propose that it should be necessary to do the following studies, in particularly, of the breeding of transgenic fish: to assess the fitness of transgenic fish in an aqueous environment with a large space and a complex structure; and to develop a controllable on-off strategy of reproduction in transgenic fish. 展开更多
关键词 TRANSGENE integration mechanism GH transgenic fish population fitness
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Corrosion performance,corrosion fatigue behavior and mechanical integrity of an extruded Mg4Zn0.2Sn alloy 被引量:3
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作者 Pingli Jiang Carsten Blawert +1 位作者 Jan Bohlen Mikhail L.Zheludkevich 《Journal of Materials Science & Technology》 SCIE EI CAS CSCD 2020年第24期107-116,共10页
Magnesium alloys are promising as load bearing components.They are inevitably exposed to cyclic loading and corrosive environment in actual service,which can consequently result in corrosion fatigue failure and loss o... Magnesium alloys are promising as load bearing components.They are inevitably exposed to cyclic loading and corrosive environment in actual service,which can consequently result in corrosion fatigue failure and loss of mechanical integrity of the material.Therefore,in the present study,the corrosion behavior,corrosion fatigue performance and mechanical integrity of an extruded Mg4Zn0.2Sn(wt.%)alloy were thoroughly studied in two corrosive electrolytes.Strong localized corrosion occurred when the alloy was immersed in deionized water based sodium chloride(NaCl)solution.The poor corrosion resistance of the alloy resulted in a fast deterioration of the tensile properties after pre-exposure to salt spray and a poor fatigue resistance in deionized water based NaCl solution.In comparison,the active dissolution of the substrate was sufficiently suppressed in artificial tap water based NaCl solution due to the formation of highly protective corrosion product layers.This consequently conferred longer fatigue life on the alloy in the electrolyte.Our results emphasized the influence of corrosion on the fatigue behavior and tensile properties of magnesium alloys. 展开更多
关键词 Magnesium alloy TIN Artificial tap water Corrosion fatigue Mechanical integrity
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T-DNA Integration Category and Mechanism in Rice Genome 被引量:2
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作者 JiangWANG LinLI Zhen-YingSHI Xin-ShanWAN Lin-ShengAN Jing-LiuZHANG 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2005年第3期350-361,共12页
Abstract: T-DNA integration is a key step in the process of plant transformation, which is proven to be important for analyzing T-DNA integration mechanism. The structures of T-DNA right borders inserted into the rice... Abstract: T-DNA integration is a key step in the process of plant transformation, which is proven to be important for analyzing T-DNA integration mechanism. The structures of T-DNA right borders inserted into the rice (Oryza sativa L.) genome and their flanking sequences were analyzed. It was found that the integrated ends of the T-DNA right border occurred mainly on five nucleotides “TGACA” in inverse repeat (IR) sequence of 25 bp, especially on the third base “A”. However, the integrated ends would sometimes lie inward of the IR sequence, which caused the IR sequence to be lost completely. Sometimes the right integrated ends appeared on the vector sequences rightward of the T-DNA right border, which made the T-DNA, carrying vector sequences, integrated into the rice genome. These results seemingly suggest that the IR sequence of the right border plays an important role in the process of T-DNA integration into the rice genome, but is not an essential element. The appearance of vector sequences neighboring the T-DNA right border suggested that before being transferred into the plant cell from Agrobacterium, the entire T-DNA possibly began from the left border in synthesis and then read through at the right border. Several nucleotides in the T-DNA right border homologous with plant DNA and filler DNAs were frequently discovered in the integrated position of T-DNA. Some small regions in the right border could match with the plant sequence, or form better matches, accompanied by the occurrence of filler DNA, through mutual twisting, and then the T-DNA was integrated into plant chromosome through a partially homologous recombination mechanism. The appearance of filler DNA would facilitate T-DNA integration. The fragments flanking the T-DNA right border in transformed rice plants could derive from different parts of the inner T-DNA region; that is, disruption and recombination could occur at arbitrary positions in the entire T-DNA, in which the homologous area was comparatively easier to be disrupted. The structure of flanking sequences of T-DNA integrated in the rice chromosome presented various complexities. These complexities were probably a result of different patterns of recombination in the integrating process. Some types of possible integrating mechanism are detailed. 展开更多
关键词 integration mechanism rice (Oryza sativa L.) right border T-DNA
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Automated ex-situ detection of pitting corrosion and its effect on the mechanical integrity of rare earth magnesium alloy-WE43 被引量:2
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作者 Kerstin van Gaalen Felix Gremse +3 位作者 Felix Benn Peter E.McHugh Alexander Kopp Ted J.Vaughan 《Bioactive Materials》 SCIE 2022年第2期545-558,共14页
This study develops a three-dimensional automated detection framework(PitScan)that systematically evaluates the severity and phenomenology of pitting corrosion.This framework uses a python-based algorithm to analyse m... This study develops a three-dimensional automated detection framework(PitScan)that systematically evaluates the severity and phenomenology of pitting corrosion.This framework uses a python-based algorithm to analyse microcomputer-tomography scans(μCT)of cylindrical specimens undergoing corrosion.The approach systematically identifies several surface-based corrosion features,enabling full spatial characterisation of pitting parameters,including pit density,pit size,pit depth as well as pitting factor according to ASTM G46-94.Furthermore,it is used to evaluate pitting formation in tensile specimens of a Rare Earth Magnesium alloy undergoing corrosion,and relationships between key pitting parameters and mechanical performance are established.Results demonstrated that several of the parameters described in ASTM G46-94,including pit number,pit density and pitting factor,showed little correlation to mechanical performance.However,this study did identify that other parameters showed strong correlations with the ultimate tensile strength and these tended to be directly linked to the reduction of the cross-sectional area of the specimen.Specifically,our results indicate,that parameters directly linked to the loss of the cross-sectional area(e.g.minimum material width),are parameters that are most suited to provide an indication of a specimen’s mechanical performance.The automated detection framework developed in this study has the potential to provide a basis to standardise measurements of pitting corrosion across a range of metals and future prediction of mechanical strength over degradation time. 展开更多
关键词 MAGNESIUM Corrosion Degradation Micro-computer-tomography Pit detection AUTOMATIZATION Mechanical integrity
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