Impacts of PI3K/protein kinase B pathway activation in reactive astrocytes: from detrimental effects to protective functions

Astrocytes are the most abundant type of glial cell in the central nervous system.Upon injury and inflammation,astrocytes become reactive and undergo morphological and functional changes.Depending on their phenotypic classification as A1 or A2,reactive astrocytes contribute to both neurotoxic and neuroprotective responses,respectively.However,this binary classification does not fully capture the diversity of astrocyte responses observed across different diseases and injuries.Transcriptomic analysis has revealed that reactive astrocytes have a complex landscape of gene expression profiles,which emphasizes the heterogeneous nature of their reactivity.Astrocytes actively participate in regulating central nervous system inflammation by interacting with microglia and other cell types,releasing cytokines,and influencing the immune response.The phosphoinositide 3-kinase(PI3K)/protein kinase B(AKT)signaling pathway is a central player in astrocyte reactivity and impacts various aspects of astrocyte behavior,as evidenced by in silico,in vitro,and in vivo results.In astrocytes,inflammatory cues trigger a cascade of molecular events,where nuclear factor-κB serves as a central mediator of the pro-inflammatory responses.Here,we review the heterogeneity of reactive astrocytes and the molecular mechanisms underlying their activation.We highlight the involvement of various signaling pathways that regulate astrocyte reactivity,including the PI3K/AKT/mammalian target of rapamycin(mTOR),αvβ3 integrin/PI3K/AKT/connexin 43,and Notch/PI3K/AKT pathways.While targeting the inactivation of the PI3K/AKT cellular signaling pathway to control reactive astrocytes and prevent central nervous system damage,evidence suggests that activating this pathway could also yield beneficial outcomes.This dual function of the PI3K/AKT pathway underscores its complexity in astrocyte reactivity and brain function modulation.The review emphasizes the importance of employing astrocyte-exclusive models to understand their functions accurately and these models are essential for clarifying astrocyte behavior.The findings should then be validated using in vivo models to ensure real-life relevance.The review also highlights the significance of PI3K/AKT pathway modulation in preventing central nervous system damage,although further studies are required to fully comprehend its role due to varying factors such as different cell types,astrocyte responses to inflammation,and disease contexts.Specific strategies are clearly necessary to address these variables effectively.

粘附分子CD44V6及Integrinαvβ_3在胆囊癌中的表达及意义

目的研究粘附分子CD44V6及Integrinαvβ_3在胆囊癌组织及胆囊炎中表达是否存在统计学意义的差异,其表达量与胆囊癌的分化程度之间的关系。方法应用免疫组织化学的方法检测20例胆囊癌及20例慢性胆囊炎组织中CD44V6及Integrinαvβ_3的表达,结合临床病理结果进行分析。结果在胆囊癌中CD44V6和Integrinαvβ_3表达阳性率分别为80.0%和70.0%。CD44V6及Integrinαvβ_3在胆囊癌中表达存在协同性,与胆囊癌分化程度呈正相关(均P<0.05)。结论 CD44V6及Integrinαvβ_3在胆囊癌中高表达(显著高于对照组),并且随着胆囊癌的分化程度降低其表达阳性分值增高;CD44V6及Integrinαvβ_3的表达与原发性胆囊癌的良恶性及分化程度相关;在相同标本中,CD44V6高表达往往伴有Integrinαvβ_3高表达,在胆囊癌的诊断上具有一致性,可联合作为临床监测胆囊癌发生、转移的参考指标。

NCG对山羊性腺轴组织中ITGB8、IGFBP3基因相对表达量的影响

为了探究妊娠早期饲喂N-氨甲酰谷氨酸(N-Carbamylglutamate,NCG)提高山羊产羔数的繁殖机制,试验采用实时荧光定量PCR方法检测了ITGB8、IGFBP3基因在空白组和NCG组(0.15%NCG)卵巢、子宫、输卵管、垂体、下丘脑等性腺轴组织中的相对表达量。结果表明:ITGB8和IGFBP3基因在贵州白山羊5个组织中均有不同程度表达。相同组别不同组织比较,ITGB8基因在空白组的相对表达量为下丘脑>垂体>输卵管>子宫>卵巢,在NCG组的相对表达量为垂体>子宫>下丘脑>输卵管>卵巢;IGFBP3基因在空白组和NCG组中的表达趋势均为子宫>卵巢>输卵管>下丘脑>垂体。相同组织不同组间比较,ITGB8基因在NCG组垂体、卵巢、输卵管、子宫组织中的相对表达量极显著低于空白组(P<0.01),在下丘脑中两组间差异不显著(P>0.05);IGFBP3基因在NCG组垂体、卵巢、输卵管、子宫组织中的相对表达量均极显著低于空白组(P<0.01),在下丘脑中两组间差异不显著(P>0.05)。说明给山羊饲喂NCG能提高ITGB8基因和降低IGFBP3基因的相对表达量,NCG可能通过调控ITGB8和IGFBP3基因的表达影响山羊繁殖性能。

Non-invasively differentiate non-alcoholic steatohepatitis by visualizing hepatic integrinαvβ3 expression with a targeted molecular imaging modality

BACKGROUND Non-invasive methods to diagnose non-alcoholic steatohepatitis(NASH),an inflammatory subtype of non-alcoholic fatty liver disease(NAFLD),are currently unavailable.AIM To develop an integrinαvβ3-targeted molecular imaging modality to differentiate NASH.METHODS Integrinαvβ3 expression was assessed in Human LO2 hepatocytes Scultured with palmitic and oleic acids(FFA).Hepatic integrinαvβ3 expression was analyzed in rabbits fed a high-fat diet(HFD)and in rats fed a high-fat,high-carbohydrate diet(HFCD).After synthesis,cyclic arginine-glycine-aspartic acid peptide(cRGD)was labeled with gadolinium(Gd)and used as a contrast agent in magnetic resonance imaging(MRI)performed on mice fed with HFCD.RESULTS Integrinαvβ3 was markedly expressed on FFA-cultured hepatocytes,unlike the control hepatocytes.Hepatic integrinαvβ3 expression significantly increased in both HFD-fed rabbits and HFCD-fed rats as simple fatty liver(FL)progressed to steatohepatitis.The distribution of integrinαvβ3 in the liver of NASH cases largely overlapped with albumin-positive staining areas.In comparison to mice with simple FL,the relative liver MRI-T1 signal value at 60 minutes post-injection of Gd-labeled cRGD was significantly increased in mice with steatohepatitis(P<0.05),showing a positive correlation with the NAFLD activity score(r=0.945;P<0.01).Hepatic integrinαvβ3 expression was significantly upregulated during NASH development,with hepatocytes being the primary cells expressing integrinαvβ3.CONCLUSION After using Gd-labeled cRGD as a tracer,NASH was successfully distinguished by visualizing hepatic integrinαvβ3 expression with MRI.

Inhibitory Effects of Alpha-zearalenol on Angiotensin II-Induced Integrin β_3 mRNA via Suppression of Nuclear Factor-κB

To investigate the effect of α-zearalenol on angiotensin If-induced β3 integrin mRNA expression in human umbilical vein endothelial cells （HUVECs）. Methods The mRNA level in integrin β3 was determined by reverse transcription-polymerase chain reaction. Endothelial NF-κB activity was determined by the luciferase activity assay of plasmid NF-κB-LUC. Results The angiotensin Ⅱ- induced β3 integrin mRNA expression was inhibited by α-zearalenol and 17β-estradiol （10nmol/L ^-1 μmol/L）, but not influenced by ICI 182, 780, a pure competitive antagonist for estrogen receptor or a nitric oxide inhibitor N^ω-Nitro-L-arginine methyl ester hydrochloride. Alpha-zearalenol and 17β-estradiol suppressed the angiotensin Ⅱ-induced activation of NF-κB in endothelial cells. Condusion Alpha-zearalenol inhibits angiotensin Ⅱ-induced integrin β3 mRNA expression by suppressing NF-κB activation in endothelial cells.

Changes of β_3 Integrins and Extracellular Matrix Proteins in the Endometrium of Unexplained Infertility

The purpose of this study was to investigate changes of β 3 integrins and extra cellular matrix proteins including fibronectin (FN), laminin (LN) and collagen type Ⅳ (CL typeⅣ) on the endometrium of secretory phase from 31 fertile women (fertility group)and 34 women with unexplained infertility (infertility group) by a histochemical method.The results were as follows:In glandular epithelium, β 3 integrin appeared in the mid secretory phase and continued to late secretory phase in the fertility group, but was not expressed during the secretory phase in the infertility group. Extracellular matrix proteins from the fertility group were expressed more strongly in mid secretory phase than that in the early secretory phase, and were weakest in the late secretory phase. Compared with the fertility group, the levels of extracellular matrix proteins in the infertility group were elevated in the secretory phase. In conclusion: our current study demonstrate that β 3 integrin and extracellular matrix proteins are expressed at different levels in the endometrium during the menstrual cycle. They are involved in endometrial changes during the menstrual cycle and during the implantation of the blastocyst. Their unusual expression result in the failure of implantation.

Ischemic preconditioning partially suppresses and postpones integrin α_Vβ_3 mRNA expression following transient global cerebral ischemia in C57BL/6 mice

Previous studies of integrin αvβ3 have focused on ischemic brain damage, although the role of integrin αvβ3 in ischemic preconditioning （IP） has rarely been reported. The present study analyzed the effects of IP on integrin αvβ3 mRNA expression following cerebral ischemia through the use of hematoxylin-eosin staining and real-time quantitative polymerase chain reaction techniques. Integrin avid3 mRNA expression in the ischemia group peaked at 24 hours after ischemia-reperfusion. In the IP ＋ ischemia group, integrin αvβ3 mRNA expression increased after 24 hours, but remained significantly less than the ischemia group, and expression continued to increase until 7 days after ischemiaJreperfusion. These results demonstrate that IP effectively attenuated upregulation of integrin αvβ3 mRNA expression at 24 hours after ischemia.

The Expression of Integrin β_3 and Intercellular Adhesion Molecule(ICAM-1) in Decidua and Chorionic Villi during Mifepristone Induced Abortion

The effects of mifepristone with misoprostol on the expression of the integrin β 3 and intercellular adhesion molecule 1 (ICAM 1) in decidua and chorionic villi tissues in early pregnancy in 10 cases were investigated by immuno flow cytometry (the experiment group). At the same time, the other 10 cases induced by mechanical vacuum aspiration were collected as the control. The results showed that, the positive rate of integrin β 3 and ICAM 1 in decidua of the experiment group were 19.1±5.01% and 20.61 ±6.51%; while those in chorionic villi were 21.32±4.38% and 20.29± 6.49%, which were significantly lower than those in the control group. These results suggested that integrin β 3 and ICAM 1 may take part in the maintenance of early pregnancy. The mechanism of mifepristone induced abortion may be mediated by the down regulation of the integrin β 3 and ICAM 1 expression in decidua and chorionic villi.

miRNA-30b调控ITGB3表达促进乳腺癌细胞凋亡

目的:探讨微小RNA-30b(microRNA-30b,miRNA-30b)和整合素β3(integrinβ3,ITGB3)在乳腺癌组织中的表达及对乳腺癌细胞生物学行为的影响。方法:选择2016年3月至2019年3月于成都医学院第一附属医院收治的144例包括原位、浸润性和转移性乳腺癌患者的组织标本以及其癌旁组织,同时收集其临床病理资料。采用实时荧光定量PCR(RT-PCR)检测miRNA-30b和ITGB3的表达。构建miRNA-30b高表达的miRNA-30b模拟物和ITGB3高表达的miRNA-30b模拟物-pc DNA3.1-ITGB3,转染乳腺癌MCF-7细胞,同时将转染后的细胞皮下注射至小鼠体内。5-溴脱氧尿嘧啶核苷(BrdU)实验、流式细胞术、Transwell小室侵袭实验检测细胞增殖、凋亡、侵袭,Western blot检测细胞中的ITGB3表达。结果:RT-PCR结果显示,原位、浸润性和转移性乳腺癌组织中的miRNA-30b相对表达量分别为0.75、0.52和0.23,ITGB3 mRNA分别为2.17、3.76和5.43,与正常组织相比,差异均具有统计学意义(均P<0.001)。miRNA-30b高表达后,BrdU、流式细胞术、Transwell小室侵袭实验、Western blot检测结果显示,癌细胞的增殖、侵袭能力以及ITGB3表达明显降低,凋亡率升高(均P<0.001)。结论:在乳腺癌组织中miRNA-30b和ITGB3表达异常,miRNA-30b过表达能明显下调ITGB3表达,抑制乳腺癌细胞的增殖、侵袭,并促使其凋亡。

整合素β_3及细胞外基质分子纤粘连蛋白、层粘连蛋白在早孕药物流产蜕膜与绒毛中的表达

为了解整合素β３ 及细胞外基质中纤粘连蛋白（ＦＮ）、层粘连蛋白（ＬＭ）在米非司酮抗早孕蜕膜与绒毛组织中的表达。将２０ 例停经≤４９ 天，要求流产的早孕妇女随机分成２ 组，对照组１０ 例，药物流产（药流）组１０ 例，应用流式细胞定性定量分析技术，对经免疫荧光标记的蜕膜、绒毛组织中整合素β３、ＦＮ、ＬＭ 进行分析测定。结果表明，药流组绒毛与蜕膜组织中整合素β３ 及ＬＭ 与正常早孕组比较，呈同步性降低（Ｐ＜ ０．００１～０．０５）；ＦＮ 则无明显变化（Ｐ＞ ０．０５）。提示整合素β３ 和ＬＭ 在维持早孕中具有重要作用，米非司酮可降低绒毛、蜕膜组织中整合素β３ 和ＬＭ

外源性LIF和IL-1对围着床期小鼠子宫内膜整合素β_3亚基表达的调节

Objective:To study regulation of external leukemia inhibitory factor(LIF) and interlukin 1(IL 1) on the expression of integrin β 3 in the mouse endometrium during peri implantation period and explore the mechanism which LIF participate in embryonic implantation.Methods:The mice were injected peritoneally with LIF,IL 1 or IL 1ra at different administration on day 2～4 of pregnancy.The level of integrin β 3 protein was measured with Westernblot method and mRNA measured with RT PCR.Results:The levels of integrin β 3 protein and mRNA were increased by LIF and IL 1(P<0.05) and which relied on administration.The level of integrin β 3 mRNA was decreased by IL 1ra (P<0.05).Conclusion:LIF and IL 1 of cytokines can improve the expression of integrin β 3 in the peri implantation endometrium of mouse.Both of them may take part in implantation by regulating cell adhesion molecules.

来曲唑与克罗米酚对子宫内膜整合素α_Vβ_3和基质金属蛋白酶组织抑制因子-3表达的影响

目的 比较来曲唑（LE）与克罗米酚（CC）促排卵治疗时对植入窗期子宫内膜整合素αvβ3和基质金属蛋白酶组织抑制因子-3（TIMP-3）表达的影响。方法 收集2004年3～10月在本中心服用LE或CC促排卵治疗20例患者植入窗期子宫内膜（LE组9例，CC组11例），另取12例自然月经周期的相应时期内膜作为对照。通过免疫组织化学法测定整合素αvβ3和TIMP-3的表达强度。结果 三组子宫内膜整合素αvβ3的表达强度无显著性差异；TIMP-3表达强度CC组高于LE和对照组。结论 LE和CC都不影响植入窗期子宫内膜中整合素αvβ3的表达。CC使TIMP-3的表达信号明显加强。

抗人整合素ανβ_3单链抗体的构建和表达

目的 :利用基因工程抗体技术构建抗人整合素ανβ3 单链抗体(scFv)。方法 :从分泌抗人整合素ανβ3 单抗 (mAb)的杂交瘤细胞E10总RNA中 ,用RT PCR扩增VH 和VL 基因 ,对其核苷酸序列分析后 ,通过PCR在VH 和VL 基因间插入柔性连接子(Gly4Ser) 3,组装成scFv基因 ,并克隆至原核表达载体pTIG TRX中。以重组子转化大肠杆菌BL2 1(DE3 )诱导目的基因表达。结果 :转化菌可表达相对分子质量 (Mr)为 3 10 0 0的scFv。Westernblot证实 ,具有His6标签蛋白的表达。经低剂量的IPTG诱导和较低温度培养 ,scFv获得了可溶性形式表达。表达产物经Ni NTA琼脂糖层析纯化后纯度达 91%以上。ELISA鉴定证实 ,scFv具有良好的抗原结合活性。结论 :成功地构建并表达抗人整合素ανβ3 的scFv ,为进一步临床研究奠定了基础。

压力诱导血管平滑肌细胞整合素β_3、桩蛋白和纽蛋白的变化

目的 研究压力对大鼠颈总动脉血管平滑肌细胞整合素 β3、桩蛋白和纽蛋白的分布与表达的影响 ,探讨它们在血管平滑肌细胞的机械应力信号转导中的作用。 方法 不同压力 (16 0mmHg和 80mmHg)灌流大鼠颈总动脉 30min ,免疫组织化学和免疫电镜观察整合素 β3、桩蛋白和纽蛋白在血管平滑肌细胞上的表达和分布变化。 结果 高压和常压灌流对大鼠颈总动脉平滑肌细胞的整合素 β3、桩蛋白和纽蛋白的表达无明显影响 ,但高压使整合素β3、桩蛋白和纽蛋白在平滑肌细胞膜上的分布发生改变 ,向离管腔面的平滑肌细胞膜聚集。 结论 高压诱导了整合素 β3、桩蛋白和纽蛋白向离管腔面的平滑肌细胞膜聚集。提示整合素 β3。

Galectin-3及整合素α_νβ_3 mRNA在脑胶质瘤细胞中的表达及其意义

目的:探讨半乳糖凝集素-3(Galectin-3)及整合素ανβ3mRNA在胶质瘤细胞中的表达及二者在胶质瘤的发生及恶性进展中的作用。方法:采用原位杂交法检测5例正常脑组织及40例不同级别胶质瘤中Galectin-3mRNA、整合素ανβ3mRNA的表达情况,并分析二者之间的关系。结果:正常脑组织中Galectin-3mRNA、整合素ανβ3mRNA的表达均为阴性。Galectin-3mRNA主要表达于脑胶质瘤细胞的胞浆中,在23例Ⅰ-Ⅱ级胶质瘤标本中阳性2例、弱阳性4例、阴性17例,阳性表达率为29.09%(6/23),阳性指数(LI)值为(4.80±3.22)%;在17例Ⅲ-Ⅳ级胶质瘤标本中强阳性4例、阳性6例、弱阳性1例、阴性6例,阳性表达率为64.71%(11/17),LI值为(27.30±22.72)%,Ⅰ-Ⅱ级胶质瘤Galectin-3mRNA阳性表达率低于Ⅲ-Ⅳ级(P<0.05)。整合素ανβ3mRNA主要表达于脑胶质瘤细胞的胞浆中,在23例Ⅰ-Ⅱ级胶质瘤标本中阳性3例、弱阳性4例、阴性16例,阳性表达率为34.43%(7/23),LI值为(5.35±3.79)%;在17例Ⅲ-Ⅳ级胶质瘤标本中强阳性4例、阳性6例、弱阳性3例、阴性4例,阳性表达率为76.47%(13/17),LI值为(29.24±21.10)%,Ⅰ-Ⅱ级胶质瘤整合素ανβ3mRNA阳性表达率低于Ⅲ-Ⅳ级(P<0.05)。直线相关分析结果显示,Galectin-3mRNA与整合素ανβ3mRNA的阳性细胞表达率呈正相关(r=0.718,P<0.05)。结论:Galectin-3mRNA、整合素ανβ3mRNA的表达在Ⅰ-Ⅱ级胶质瘤组明显低于Ⅲ-Ⅳ级胶质瘤组,提示Galectin-3及整合素ανβ3mRNA检测可作为从分子水平判定人脑胶质瘤恶性生物学行为的指标。

miR-143-3p通过靶向integrinβ1抑制肝癌进展

目的探讨肝癌中miR-143-3p的表达及其对肝癌细胞恶性生物学行为的影响,并分析潜在的机制。方法收集肝癌组织,用RT-qPCR和Western blot法分别检测miR-143-3p和integrinβ1的表达。体外培养肝癌细胞,并用miR-143-3p mimics转染后,用XTT和EDU法检测过表达miR-143-3p对细胞增殖活力的影响,用Transwell法检测过表达miR-143-3p对细胞迁移与侵袭的影响,Western blot法检测过表达miR-143-3p对integrinβ1表达的影响。用荧光素酶活性法检测miR-143-3p与integrinβ1的靶向关系。用XTT、EdU和Transwell法检测过表达integrinβ1对已转染miR-143-3p mimics的肝癌细胞增殖、迁移与侵袭的影响。结果与癌旁正常组织比较,肝癌组织中miR-143-3p表达降低,integrinβ1表达升高,且二者均随疾病分期进展进一步降低或增高。过表达miR-143-3p能抑制肝癌细胞增殖、迁移和侵袭,并能下调integrinβ1表达。过表达integrinβ1能逆转miR-143-3p对肝癌细胞的抑制作用。integrinβ1为miR-143-3p的靶点。结论miR-143-3p在肝癌中表达下调,而上调miR-143-3p能通过靶向integrinβ1抑制肝癌细胞增殖、迁移与侵袭。

黄体功能不全患者子宫内膜上皮细胞整合素β_3亚单位与孕激素受体关系

目的 探讨黄体功能不全患者子宫接受性与上皮细胞雌、孕激素受体的关系。方法 应用免疫组化方法测定黄体功能不全患者子宫内膜上皮细胞整合素β3亚单位和雌、孕激素受体的表达。结果 黄体功能不全患者子宫内膜上皮细胞整合素 β3亚单位含量显著降低 ,而雌、孕激素受体含量明显升高。结论 黄体功能不全患者子宫接受性下降 ,且与黄体中期雌、孕激素受体失去下调密切相关。

血栓通与阿司匹林对ITGB3基因多态性药物抵抗患者治疗作用的研究

目的探讨中药制剂血栓通及阿司匹林抗血小板治疗脑卒中以及ITGB3基因多态性对治疗效果的相关性。方法收集了192例脑梗死患者,随机分为4组(阿司匹林组、血栓通组、血栓通+阿司匹林组、阿司匹林+氯吡格雷组)进行治疗,通过一般资料及测定生化指标和血栓弹力图,同时提取静脉血全基因组DNA,采用Snapshot方法检测ITGB3基因型,分析4组患者的血小板抑制情况,比较各组结果。结果整体水平上,各组经药物治疗后,血小板抑制率显著提高,血栓通治疗对3种ITGB3基因型AA、AG、GG患者血小板抑制率显著高于阿司匹林组,且组间没有显著性差异。结论中药制剂血栓通可以抑制血小板聚集发挥药效,并且突变型ITGB3基因AA及GG不会对血栓通产生抵抗。

妊娠期高血压疾病患者胎盘组织中基质金属蛋白酶-9(MMP-9)和整合素β 3(Integrin β 3)的表达

目的研究基质金属蛋白酶-9(MMP-9)和整合素β3(Integrin β3)在妊娠期高血压疾病患者胎盘组织中的表达,并探讨其与妊娠期高血压疾病发病的关系及两者的相关性。方法采用免疫组织化学染色(SP法)分别检测In-tegrinβ3、MMP-9在10例妊娠期高血压疾病患者及10例正常妊娠者胎盘组织中的表达。结果妊娠期高血压疾病组胎盘Integrinβ3表达明显高于正常妊娠组(P<0.01)。妊娠期高血压疾病组胎盘MMP-9表达明显低于正常妊娠组(P<0.01),且随病情的加重MMP-9的表达有下降趋势,有统计学意义(P<0.05)。结论本研究显示:与正常妊娠组比较妊娠期高血压疾病患者胎盘组织integrinβ3表达明显增加、MMP-9的表达明显减少,且随病情加重有减少趋势。表明integrinβ3、MMP-9可能参与妊娠期高血压疾病的发生和发展。

壮骨镇痛胶囊对裸鼠乳腺癌移植瘤模型骨转移指标ITG-αvβ_3及BSP的影响

目的:从实验角度论证壮骨镇痛胶囊预防乳腺癌发生骨转移的疗效,并从肿瘤黏附、浸润转移及分子水平初步探讨产生疗效的机理,为壮骨镇痛胶囊防治乳腺癌骨转移提供有力的药效学实验依据。方法:采用肩胛部皮下接种的方法建立裸鼠乳腺癌移植瘤模型,观察壮骨镇痛胶囊对裸鼠乳腺癌移植瘤模型骨转移相关指标整联蛋白αvβ(3ITG-αvβ3)及骨唾液酸蛋白(BSP)表达的影响。结果:壮骨镇痛胶囊可以显著降低移植瘤组织中ITG-αvβ3及BSP的表达,与模型组比较,差异具有统计学意义(P<0.05)。结论:壮骨镇痛胶囊具有预防和治疗乳腺癌骨转移的作用,其机制可能与调控ITG-αvβ3及BSP的表达,抑制肿瘤细胞黏附、浸润转移有关。