Phosphorylated protein chip combined with artificial intelligence tools for precise drug screening

We have developed a protein array system,named"Phospho-Totum",which reproduces the phosphorylation state of a sample on the array.The protein array contains 1471 proteins from 273 known signaling pathways.According to the activation degrees of tyrosine kinases in the sample,the corresponding groups of substrate proteins on the array are phosphorylated under the same conditions.In addition to measuring the phosphorylation levels of the 1471 substrates,we have developed and performed the artificial intelligence-assisted tools to further characterize the phosphorylation state and estimate pathway activation,tyrosine kinase activation,and a list of kinase inhibitors that produce phosphorylation states similar to that of the sample.The Phospho-Totum system,which seamlessly links and interrogates the measurements and analyses,has the potential to not only elucidate pathophysiological mechanisms in diseases by reproducing the phosphorylation state of samples,but also be useful for drug discovery,particularly for screening targeted kinases for potential drug kinase inhibitors.

Progress and Development Direction of “Three-High” Oil and Gas Well Testing Technology

By reviewing the development of “three-high” oil and gas well testing technology of Sinopec in recent years, this paper systematically summarizes the application of “three-high” oil and gas well testing technology of Sinopec in engineering optimization design technology, and high temperature and high pressure testing technology, high pressure and high temperature transformation completion integration technology. Major progress has been made in seven aspects: plug removal and re-production technology of production wells in high acid gas fields;wellbore preparation technology of ultra-deep, high-pressure, and high-temperature oil and gas wells;surface metering technology;and supporting tool development technology. This paper comprehensively analyzes the challenges faced by the “three-high” oil and gas well production testing technology in four aspects: downhole tools, production testing technology, safe production testing, and the development of low-cost production test tools. Four development directions are put forward: 1) Improve ultra-deep oil and gas testing technology and strengthen integrated geological engineering research. 2) Deepen oil and gas well integrity evaluation technology to ensure the life cycle of oil and gas wells. 3) Carry out high-end, customized, and intelligent research on oil test tools to promote the low-cost and efficient development of ultra deep reservoirs. 4) Promote the fully automatic control of the surface metering process to realize the safe development of “three-high” reservoirs.

MODULE DEFINITION MODEL FOR MODULAR DESIGN AND MANUFACTU－RING OF HEAVY DUTY MACHINE TOOLS

The key techniques of modular design of heavy duty NC mathine tools are described. Amodule definition modelfor modular design and manufacturing of heavy duty NC machine tools isbulit and the essential composition of the module definition model (MDM) is discussed in detail. Itis composed of two models: the part definition model (PDM) and the module assembly model(MAM). The PDM and MAM are built and their structures are given. Using object-oriented know-ledge representation and based on these models, an intelligent support system of modular design forheavy duty NC machine tools is developed and implemented This system has been applied to thepractical use of Wuhan Heavy Duty Machine Tool Works

An Intelligent Tool to Support Requirements Analysis and Conceptual Design of Database Design

As an application of artificial intelligence and expert system technology to database design,this paper presents an intelligent design tool NITDT,which comprises a requirements specification lan- guage NITSL,a knowledge representation language NITKL,and an inference engine with uncertainty reasoning capability.NITDT now covers the requirements analysis and conceptual design of database design.However,it is possible to be integrated with another database design tool, NITDBA,developed also at NIT to become an integrated design tool supporting the whole process of database design.