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Cloning of CRABS CLAW Gene from Brassica napus and Construction of Its RNA Interference Expression Vector
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作者 Xiaoying ZHOU Sanxiong FU +2 位作者 Song CHEN Chao ZHANG Cunkou QI 《Agricultural Biotechnology》 CAS 2016年第1期11-14,18,共5页
CRABS ClAW (CRC) is a member of the YABBYA transcription factor gene family that plays an important role in floral organ development of plants. This study aimed to further investigate the regulatory function of CRC ... CRABS ClAW (CRC) is a member of the YABBYA transcription factor gene family that plays an important role in floral organ development of plants. This study aimed to further investigate the regulatory function of CRC transcription factor in the development of floral organs of rape (Brassica napus L. ). A 580 bp fragment of CRC gene was cloned by RT-PCR from total RNA of buds of rape cultivar Ningyou No. 10 to construct an inverted repeated expression cassette of CRC gene using intermediate vector pHturieane. Firstly, CRC gene fragment was positively inserted into the 5' end of a spliceable intron and negatively inserted into the 3' end of the intron. Subsequently, CaMV35S promoter sequence and inverted repeated expression cassette of CRC gene were transferred into pUC18 multiple clone site of binary expression vector pCAMBIAI1390. The constructed interference expression vector was named pA6-CRCi, which was further confirmed by restriction enzyme digestion and sequencing. 展开更多
关键词 wBrassica napus L. CRABS CLAW gene RNA interference expression vector
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