糖尿病的发生率逐渐升高,尤其是2型糖尿病。随着糖尿病病程的增加,各项并发症的发病率也随之升高。尤其是2型糖尿病导致的慢性并发症,对人们的健康、心理及经济等各个方面均有一定的影响。近几年的研究均表明了脂质代谢对糖尿病的发病...糖尿病的发生率逐渐升高,尤其是2型糖尿病。随着糖尿病病程的增加,各项并发症的发病率也随之升高。尤其是2型糖尿病导致的慢性并发症,对人们的健康、心理及经济等各个方面均有一定的影响。近几年的研究均表明了脂质代谢对糖尿病的发病机制及其并发症的发生及进展有一定的影响。载脂蛋白B/A-1比值作为新的代谢紊乱标志物的指标之一,可评价糖尿病患者血管相关并发症。本文就载脂蛋白B/A-1比值在2型糖尿病血管并发症中的作用的相关研究进行综述。The prevalence of type 2 diabetes is on the rise, leading to an increase in associated complications over time. In particular, the chronic complications caused by type 2 diabetes have a certain impact on people’s health, psychology and economy. Recent studies have highlighted the impact of lipid metabolism on the development and progression of diabetes-related complications. Given that the ApoB/A-1 ratio can serve as a valuable indicator for assessing metabolic disorders and evaluating chronic complications in patients with diabetes. This review provides an overview of relevant research concerning the role of ApoB/A-1 ratio in vascular complications of type 2 diabetes.展开更多
At present,the role of many long non-coding RNAs(lncRNAs)as tumor suppressors in the formation and development of cervical cancer(CC)has been studied.However,lncRNA prostate cancer gene expression marker 1(PCGEM1),who...At present,the role of many long non-coding RNAs(lncRNAs)as tumor suppressors in the formation and development of cervical cancer(CC)has been studied.However,lncRNA prostate cancer gene expression marker 1(PCGEM1),whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression,has not been studied in CC.The objective of this study was to investigate the expression and the underlying role of PCGEM1 in CC.The relative expression of PCGEM1 in CC cells was detected by real-time PCR.After the suppression of PCGEM1 expression by shRNA,the changes in the proliferation,migration,and invasion capacities were detected via CCK-8 assay,EdU assay,and colony formation assay wound healing assay.Transwell assay and the changes in expressions of epithelial-to-mesenchymal transition(EMT)markers were determined by western blot and immunofluorescence.The interplay among PCGEM1,miR-642a-5p,and kinesin family member 5B(KIF5B)was confirmed by bioinformatics analyses and luciferase reporter assay.Results showed that PCGEM1 expressions were up-regulated within CC cells.Cell viabilities,migration,and invasion were remarkably reduced after the suppression of PCGEM1 expression by shRNA in Hela and SiHa cells.N-cadherin was silenced,but E-cadherin expression was elevated by sh-PCGEM1.Moreover,by sponging miR-642a-5p in CC,PCGEM1 was verified as a competitive endogenous RNA(ceRNA)that modulates KIF5B levels.MiR-642a-5p down-regulation partially rescued sh-PCGEM1’s inhibitory effects on cell proliferation,migration,invasion,and EMT process.In conclusion,the PCGEM1/miR-642a-5p/KIF5B signaling axis might be a novel therapeutic target in CC.This study provides a research basis and new direction for targeted therapy of CC.展开更多
文摘糖尿病的发生率逐渐升高,尤其是2型糖尿病。随着糖尿病病程的增加,各项并发症的发病率也随之升高。尤其是2型糖尿病导致的慢性并发症,对人们的健康、心理及经济等各个方面均有一定的影响。近几年的研究均表明了脂质代谢对糖尿病的发病机制及其并发症的发生及进展有一定的影响。载脂蛋白B/A-1比值作为新的代谢紊乱标志物的指标之一,可评价糖尿病患者血管相关并发症。本文就载脂蛋白B/A-1比值在2型糖尿病血管并发症中的作用的相关研究进行综述。The prevalence of type 2 diabetes is on the rise, leading to an increase in associated complications over time. In particular, the chronic complications caused by type 2 diabetes have a certain impact on people’s health, psychology and economy. Recent studies have highlighted the impact of lipid metabolism on the development and progression of diabetes-related complications. Given that the ApoB/A-1 ratio can serve as a valuable indicator for assessing metabolic disorders and evaluating chronic complications in patients with diabetes. This review provides an overview of relevant research concerning the role of ApoB/A-1 ratio in vascular complications of type 2 diabetes.
基金the Nantong Municipal Health Commission Research Project(MB2021054)for this study.
文摘At present,the role of many long non-coding RNAs(lncRNAs)as tumor suppressors in the formation and development of cervical cancer(CC)has been studied.However,lncRNA prostate cancer gene expression marker 1(PCGEM1),whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression,has not been studied in CC.The objective of this study was to investigate the expression and the underlying role of PCGEM1 in CC.The relative expression of PCGEM1 in CC cells was detected by real-time PCR.After the suppression of PCGEM1 expression by shRNA,the changes in the proliferation,migration,and invasion capacities were detected via CCK-8 assay,EdU assay,and colony formation assay wound healing assay.Transwell assay and the changes in expressions of epithelial-to-mesenchymal transition(EMT)markers were determined by western blot and immunofluorescence.The interplay among PCGEM1,miR-642a-5p,and kinesin family member 5B(KIF5B)was confirmed by bioinformatics analyses and luciferase reporter assay.Results showed that PCGEM1 expressions were up-regulated within CC cells.Cell viabilities,migration,and invasion were remarkably reduced after the suppression of PCGEM1 expression by shRNA in Hela and SiHa cells.N-cadherin was silenced,but E-cadherin expression was elevated by sh-PCGEM1.Moreover,by sponging miR-642a-5p in CC,PCGEM1 was verified as a competitive endogenous RNA(ceRNA)that modulates KIF5B levels.MiR-642a-5p down-regulation partially rescued sh-PCGEM1’s inhibitory effects on cell proliferation,migration,invasion,and EMT process.In conclusion,the PCGEM1/miR-642a-5p/KIF5B signaling axis might be a novel therapeutic target in CC.This study provides a research basis and new direction for targeted therapy of CC.
