Study on pharmacodynamics of recombinant interferon α-2b suppository

Objective To investigate the antiviral activity of recombinant interferonα-2b suppository(IFNα-2b)in vivo and in vitro.Methods The cytopathic-effect inhibition assay was applied in this study to investigate the antiviral activity of this drug as well as yingtelong and axiluowei as positive control.The guinea pig model of vaginitis and skin infection caused by HSV-2 infection were established,treated with IFNα-2b suppository at dosages of 60000、180000、540000 IU,using IFNα-2b injection 180000 IU·kg-1 as controls.Score the pathological changes of appearance and skin,the virus activities of vaginal secretion and tissue sections of viginae were assayed after treatment.Results The TD50 of IFN α-2b and yingtelong for Vero cells was(>100)μg·mL-1 and(>100000)IU·mL-1,respectively.The IC50 of IFN α-2b and yingtelong and axiluowei for Herpes virus type 1 was(0.29±0.08)μg·mL-1 and(185.0±28.8)IU·mL-1 and(0.19±0.03)μg·mL-1,respectively.The mean scores for vaginal and skin lesion of the treated groups were lower than those of untreated group.Among these concentrations,the IFNα-2b suppository of 540000 IU·kg-1 group.Showed highest anti-viral activity.The virus activity in vaginal secretion of treated group was lower than that of untreated group too(P<0.01 or P<0.05).Tissue sections of viginae after treatment with IFNα-2b suppository showed significantly therapeutical effects on the degrees of vaginal lesion.At the same dosage,The anti-HSV activity of IFNα-2b suppository was also compared with IFNα-2b injection,the results showed that the activity of suppository of 540000 IU·kg-1 group was similar to that of the injection.Conclusions The IFNα-2b suppository has anti-viruses function both in vivo and in vitro.

LEEP刀宫颈锥切术联合术前术后应用重组α-2b干扰素栓治疗宫颈上皮内瘤变临床疗效分析

目的：观察LEEP刀宫颈锥切术联合术前术后应用重组α-2b干扰素栓治疗宫颈上皮内瘤变的临床效果。方法：选取2012年1月～2015年1月本站收治的宫颈上皮内瘤120例，将其按照随机数字法随机分为观察组和对照组各60例，两组均采用LEEP刀宫颈锥切术，观察组采用术前术后应用重组α-2b干扰素栓治疗，对照组仅术后应用重组α-2b干扰素栓治疗，对比分析两组的临床疗效及复发情况。结果：治疗半年后观察组总有效率（93．3％）高于对照组（76．7％），差异具有统计学意义（x^2=6．536，P〈0．05）；且随访1年观察组复发率（0％）低于对照组（6．7％），差异具有统计学意义（x^2=4．138，P〈0．05）。观察组术中出血量、阴道排液量及平均愈合时间均短于对照组（P均〈0．05）；治疗后IgG、IgA、IgM、CD4^＋、CD4^＋／CD8^＋水平均高于对照组，CD8^＋水平低于对照组（P均〈0．05）。结论：LEEP宫颈锥切术联合术前术后应用重组α-2b干扰素治疗宫颈上皮内瘤变治愈率高，术后1年复发率低，值得临床应用。