Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon tr...Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.展开更多
Objective:To know whether the effect of interferon-induced protein with tetratricopeptide repeats(IFIT)1 polymorphism influences the susceptibility of cerebral malaria outcome.Methods:Case-control association study wa...Objective:To know whether the effect of interferon-induced protein with tetratricopeptide repeats(IFIT)1 polymorphism influences the susceptibility of cerebral malaria outcome.Methods:Case-control association study was performed among 314 Thai patients(110 with cerebral malaria and 204 with uncomplicated malaria)infected with Plasmodium falciparum.Genotyping for five tag-single nucleotide polymorphisms of IFIT1 was performed by endpoint genotyping.Results:Genotype frequencies of all tag-SNPs(single nucleotide polymorphisms)showed no association with malaria outcome.However,C allele of rs11203109 was associated with the protection from cerebral malaria(OR=0.62,95%CI=0.38-0.99,P=0.048).Two single nucleotide polymorphisms(rs5786868 and rs57941432)were in linkage disequilibrium with rs11203109.Conclusions:This suggests that our associated single nucleotide polymorphism(rs11203109)might be a genetic marker of cerebral malaria progression in the Thai population.展开更多
目的探讨干扰素诱导蛋白5(interferon-induced protein with tetratricopeptide repeats 5,IFIT5)在肝癌组织中的表达、临床意义及可能的分子机制。方法收集2013年在西安交通大学第一附属医院确诊的97例肝癌患者的肿瘤组织和癌旁组织。...目的探讨干扰素诱导蛋白5(interferon-induced protein with tetratricopeptide repeats 5,IFIT5)在肝癌组织中的表达、临床意义及可能的分子机制。方法收集2013年在西安交通大学第一附属医院确诊的97例肝癌患者的肿瘤组织和癌旁组织。实时定量PCR、Western blot及免疫组化检测IFIT5在肝癌及癌旁组织中的表达水平,并分析IFIT5表达水平与临床病理指标的关系及生存率。Transwell实验检测IFIT5对HCC细胞的侵袭及迁移作用,Western blot法检测IFIT5对HCC细胞EMT的影响。结果IFIT5在肝癌组织中表达水平显著高于相应癌旁组织(P<0.05);肝癌组织IFIT5高表达与TNM分期(P=0.018)及血管侵犯(P=0.009)显著相关;高表达IFIT5患者5年生存率明显高于低表达组(P<0.05);IFIT5可显著促进HCC细胞的侵袭及迁移(P<0.05);IFIT5促进HCC细胞EMT的发生(P<0.05)。结论IFIT5在肝癌组织中表达升高,其高表达与肝癌恶性临床病理特征有关,IFIT5能够通过调控HCC细胞EMT促进其侵袭和迁移。展开更多
Objective Current commercially available immunological tests cannot be used for discriminating active tuberculosis(TB)from latent TB infection.To evaluate the value of biomarker candidates in the diagnosis of active T...Objective Current commercially available immunological tests cannot be used for discriminating active tuberculosis(TB)from latent TB infection.To evaluate the value of biomarker candidates in the diagnosis of active TB,this study aimed to identify differentially expressed genes in peripheral blood mononuclear cells(PBMCs)between patients with active TB and individuals with latent TB infection by transcriptome sequencing.Methods The differentially expressed genes in unstimulated PBMCs and in Mycobacterium tuberculosis(Mtb)antigen-stimulated PBMCs from patients with active TB and individuals with latent TB infection were identified by transcriptome sequencing.Selected candidate genes were evaluated in cohorts consisting of 110 patients with TB,30 individuals with latent TB infections,and 50 healthy controls by quantitative real-time RT-PCR.Receiver operating characteristic(ROC)curve analysis was performed to calculate the diagnostic value of the biomarker candidates.Results Among the differentially expressed genes in PBMCs without Mtb antigen stimulation,interferon-induced protein with tetratricopeptide repeats 3(IFIT3)had the highest area under curve(AUC)value(0.918,95%CI:0.852-0.984,P<0.0001)in discriminating patients with active TB from individuals with latent TB infection,with a sensitivity of 91.86%and a specificity of 84.00%.In Mtb antigen-stimulated PBMCs,orosomucoid 1(ORM1)had a high AUC value(0.833,95%CI:0.752-0.915,P<0.0001),with a sensitivity of 81.94%and a specificity of 70.00%.Conclusion IFIT3 and ORM1 might be potential biomarkers for discriminating active TB from latent TB infection.展开更多
基金supported by the Shandong Breeding Project (No. 2016LZGC009)the Projects from Laboratory for Marine Fisheries Science and Food Production Processes+2 种基金Pilot National Laboratory for Marine Science and Technology (Qingdao)(Nos. 2018-MFS-T08, 2017A STCP-OS15)the Central Public-interest Scientific Institution Basal Research Fund,CAFS (No. 2020TD20)the Central Public-Interest Scientific Institution Basal Re-search Fund,YSFRI,CAFS (No. 20603022018026)。
文摘Interferon-induced protein with tetratricopeptide repeats 1(IFIT1), also known as interferon-induced protein 56(IFI56) or Interferon-stimulated protein 56(ISG56), was originally identified as a protein induced upon treatment with interferon and inhibited by viral replication and translational initiation. In this study, Epinephelus lanceolatus IFIT1(ELIFIT1) gene was cloned for the first time. The complete cDNA of El IFIT1 gene includes 2921 nucleotides, and encodes a 437-amino acid(AA) protein. The putative ELIFIT1 protein has 9 TRP domains and is highly similar with IFIT1 proteins in other teleosts. In healthy fish, ELIFIT1 gene was highly expressed in the blood, which indicate its specific function in the peripheral immune system. Its expression was also observed in various immunity-related tissues including spleen, intestine, and kidney, Inducted with spotted knifejaw iridovirus(SKIV), ELIFIT1 gene expression was upregulated in the spleen, kidney, and liver 24 h after induction and reached its peak at 72 h, indicating that ELIFIT1 may play an important role in antivirus. These findings contribute to the understanding of the antiviral regulation of ELIFIT1 gene in teleost.
基金financially supported by the Thailand Research Fund(TRF)Office of the Higher Education Commission,the Faculty of Medical Technology to PN(MRG5480062)
文摘Objective:To know whether the effect of interferon-induced protein with tetratricopeptide repeats(IFIT)1 polymorphism influences the susceptibility of cerebral malaria outcome.Methods:Case-control association study was performed among 314 Thai patients(110 with cerebral malaria and 204 with uncomplicated malaria)infected with Plasmodium falciparum.Genotyping for five tag-single nucleotide polymorphisms of IFIT1 was performed by endpoint genotyping.Results:Genotype frequencies of all tag-SNPs(single nucleotide polymorphisms)showed no association with malaria outcome.However,C allele of rs11203109 was associated with the protection from cerebral malaria(OR=0.62,95%CI=0.38-0.99,P=0.048).Two single nucleotide polymorphisms(rs5786868 and rs57941432)were in linkage disequilibrium with rs11203109.Conclusions:This suggests that our associated single nucleotide polymorphism(rs11203109)might be a genetic marker of cerebral malaria progression in the Thai population.
文摘目的探讨干扰素诱导蛋白5(interferon-induced protein with tetratricopeptide repeats 5,IFIT5)在肝癌组织中的表达、临床意义及可能的分子机制。方法收集2013年在西安交通大学第一附属医院确诊的97例肝癌患者的肿瘤组织和癌旁组织。实时定量PCR、Western blot及免疫组化检测IFIT5在肝癌及癌旁组织中的表达水平,并分析IFIT5表达水平与临床病理指标的关系及生存率。Transwell实验检测IFIT5对HCC细胞的侵袭及迁移作用,Western blot法检测IFIT5对HCC细胞EMT的影响。结果IFIT5在肝癌组织中表达水平显著高于相应癌旁组织(P<0.05);肝癌组织IFIT5高表达与TNM分期(P=0.018)及血管侵犯(P=0.009)显著相关;高表达IFIT5患者5年生存率明显高于低表达组(P<0.05);IFIT5可显著促进HCC细胞的侵袭及迁移(P<0.05);IFIT5促进HCC细胞EMT的发生(P<0.05)。结论IFIT5在肝癌组织中表达升高,其高表达与肝癌恶性临床病理特征有关,IFIT5能够通过调控HCC细胞EMT促进其侵袭和迁移。
基金supported by grants from the Thirteen-Fifth Mega-Scientific Project on“Prevention and Treatment of AIDS,Viral Hepatitis and Other Infectious Diseases”(No.2017ZX10201301-007-002)the National Natural Science Foundation of China(No.82072233).
文摘Objective Current commercially available immunological tests cannot be used for discriminating active tuberculosis(TB)from latent TB infection.To evaluate the value of biomarker candidates in the diagnosis of active TB,this study aimed to identify differentially expressed genes in peripheral blood mononuclear cells(PBMCs)between patients with active TB and individuals with latent TB infection by transcriptome sequencing.Methods The differentially expressed genes in unstimulated PBMCs and in Mycobacterium tuberculosis(Mtb)antigen-stimulated PBMCs from patients with active TB and individuals with latent TB infection were identified by transcriptome sequencing.Selected candidate genes were evaluated in cohorts consisting of 110 patients with TB,30 individuals with latent TB infections,and 50 healthy controls by quantitative real-time RT-PCR.Receiver operating characteristic(ROC)curve analysis was performed to calculate the diagnostic value of the biomarker candidates.Results Among the differentially expressed genes in PBMCs without Mtb antigen stimulation,interferon-induced protein with tetratricopeptide repeats 3(IFIT3)had the highest area under curve(AUC)value(0.918,95%CI:0.852-0.984,P<0.0001)in discriminating patients with active TB from individuals with latent TB infection,with a sensitivity of 91.86%and a specificity of 84.00%.In Mtb antigen-stimulated PBMCs,orosomucoid 1(ORM1)had a high AUC value(0.833,95%CI:0.752-0.915,P<0.0001),with a sensitivity of 81.94%and a specificity of 70.00%.Conclusion IFIT3 and ORM1 might be potential biomarkers for discriminating active TB from latent TB infection.
