Interleukin （IL）-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are ...Interleukin （IL）-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are possibly assodated with liver disease susceptibility or se-verity. Recombinant human IL-10 has been produced and is currently tested in clinical trials. These trials may give new insights into the immunobiology of IL-10 and suggest that the IL-10/IL-10 receptor system may become a new therapeutic target.展开更多
BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased s...BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased serum concentrations of Interleukin 6(IL-6)have been associated with insulin resistance,type 2 diabetes mellitus as well as advanced forms of liver disease in chronic hepatitis C infection.AIM To investigate the frequency of IL-6-174G/C(rs1800795)single nucleotide polymorphism(SNP)in CHC patients and in healthy subjects of the same ethnicity.Associations between type 2 diabetes mellitus(dependent variable)and demographic,clinical,nutritional,virological and,IL-6 genotyping data were also investigated in CHC patients.METHODS Two hundred and forty-five patients with CHC and 179 healthy control subjects(blood donors)were prospectively included.Type 2 diabetes mellitus was diagnosed according to the criteria of the American Diabetes Association.Clinical,biochemical,histological and radiological methods were used for the diagnosis of the liver disease.IL-6 polymorphism was evaluated by Taqman SNP genotyping assay.The data were analysed by logistic regression models.RESULTS Type 2 diabetes mellitus,blood hypertension and liver cirrhosis were observed in 20.8%(51/245),40.0%(98/245)and 38.4%(94/245)of the patients,respectively.The frequency of the studied IL-6 SNP did not differ between the CHC patients and controls(P=0.81)and all alleles were in Hardy-Weinberg equilibrium(P=0.38).In the multivariate analysis,type 2 diabetes mellitus was inversely associated with GC and CC genotypes of IL-6-174(OR=0.42;95%CI=0.22-0.78;P=0.006)and positively associated with blood hypertension(OR=5.56;95%CI=2.79-11.09;P<0.001).CONCLUSION This study was the first to show that GC and CC genotypes of IL-6-174 SNP are associated with a decreased risk of type 2 diabetes mellitus in patients chronically infected with hepatitis C virus.The identification of potential inflammatory mediators involved in the crosstalk between hepatitis C virus and the axis pancreas-liver remains important issues that deserve further investigations.展开更多
目的研究慢性乙型肝炎病毒(Hepatitis B Virus,HBV)感染者血清甲状腺激素、抗甲状腺自身抗体及白细胞介素(interleukin,IL)-6水平变化及其临床意义。方法选取健康对照者18例、慢性乙型肝炎(CHB)患者65例和乙型肝炎肝硬化患者34例,应用...目的研究慢性乙型肝炎病毒(Hepatitis B Virus,HBV)感染者血清甲状腺激素、抗甲状腺自身抗体及白细胞介素(interleukin,IL)-6水平变化及其临床意义。方法选取健康对照者18例、慢性乙型肝炎(CHB)患者65例和乙型肝炎肝硬化患者34例,应用电化学发光法分别检测并比较血清甲状腺激素水平、促甲状腺激素(TSH)、抗甲状腺相关抗体及IL-6水平。结果 CHB患者抗甲状腺过氧化物酶抗体(TPOAb)、抗甲状腺球蛋白抗体(TGAb)、抗促甲状腺激素受体抗体(TRAb)和IL-6水平分别为(9.5±5.6)KIU/L、(42.5±218.7)KIU/L、(1.5±0.7)IU/L和(6.6±12.6)pg/mL,在肝硬化患者分别为(9.5±8.6)KIU/L、(47.6±101.5)KIU/L、(2.2±1.7)IU/L和(15.8±25.5)pg/mL,均显著高于正常组[分别为(4.4±5.6)KIU/L、(7.7±18.3)KIU/L、(0.8±0.8)IU/L和(2.8±2.0)pg/mL,P<0.05],而CHB和肝硬化患者血FT3分别为(2.4±0.5)ng/L和(2.4±0.6)ng/L,均显著低于对照组[(2.9±0.2)ng/L,P<0.05];肝硬化患者TRAb和IL-6水平分别为(2.2±1.7)IU/L和(15.8±25.5)pg/mL,明显高于CHB患者[(1.5±0.7)IU/L和(6.6±12.6)pg/mL,P<0.05],而TT3和TT4水平分别为(0.8±0.3)nmol/L和(5.4±1.9)nmol/L,显著低于CHB患者[(1.3±0.3)nmol/L和(8.0±2.2)nmol/L,P<0.05]。结论联合检测慢性HBV感染者血清甲状腺激素、抗甲状腺自身抗体及IL-6水平对病情评价和预后判断有一定的临床价值。展开更多
目的：检测膝骨关节炎（osteoarthritis，OA）患者血清及关节液中白细胞介素-17（in-terleukin-17）的表达并评估其与 OA 严重程度的相关性。方法收集100例原发性膝 OA 患者血清及关节液标本，并以50例健康志愿者血清标本作为对照。OA ...目的：检测膝骨关节炎（osteoarthritis，OA）患者血清及关节液中白细胞介素-17（in-terleukin-17）的表达并评估其与 OA 严重程度的相关性。方法收集100例原发性膝 OA 患者血清及关节液标本，并以50例健康志愿者血清标本作为对照。OA 严重程度及分级分别采用 Lequesne指数和 K-L 分级系统。酶联免疫吸附法分别检测血清及关节液中 IL-17浓度，并分析其与 OA 严重程度的相关性。结果OA 组患者血清中 IL-17浓度[（5．292±1．470）pg／ml]明显高于对照组[（4．173±0．640）pg／ml]，差异有统计学意义（P ＜0．05）。K-L4级或3级 OA 患者关节液 IL-17的浓度明显高于2级 OA 患者，差异有统计学意义（P ＜0．05）。4级 OA 患者关节液 IL-17的浓度[（6．161±1．120）pg／ml]明显高于3级 OA 患者[（4．474±0．816）pg／ml]，差异有统计学意义（P ＜0．05）。关节液 IL-17浓度与 Lequesne 指数呈正相关（r ＝0．6232，P ＜0．05）；血清 IL-17浓度与 Lequesne 指数之间无明显相关。结论IL-17在 OA 患者血清及关节液中的表达明显升高，关节液中 IL-17的水平与 OA 严重程度之间明显相关，提示 IL-17的失衡表达在 OA 的发病机制中具有一定作用。展开更多
Chronic activation of microglial cells endangers neuronal survival through the release of various proinflammatory and neurotoxic factors. The root of Paeonia lactiflora Pall has been considered useful for the treatmen...Chronic activation of microglial cells endangers neuronal survival through the release of various proinflammatory and neurotoxic factors. The root of Paeonia lactiflora Pall has been considered useful for the treatment of various disorders in traditional oriental medicine. Paeonol, found in the root of Paeonia lactiflora Pall, has a wide range of pharmacological functions, including anti-oxidative, anti-inflammatory and neuroprotective activities. The objective of this study was to examine the efficacy of paeonol in the repression of inflammation-induced neurotoxicity and microglial cell activation. Organotypic hippocampal slice cultures and primary microglial cells from rat brain were stimulated with bacterial lipopolysaccharide. Paeonol pretreatment was performed for 30 minutes prior to lipopolysaccharide addition. Cell viability and nitrite （the production of nitric oxide）, tumor necrosis factor-alpha and interleukin-lbeta products were measured after lipopolysaccharide treatment. In organotypic hippocampal slice cultures, paeonol blocked lipopolysaccharide-related hippocampal cell death and inhibited the release of nitrite and interleukin-lbeta. Paeonol was effective in inhibiting nitric oxide release from primary microglial cells. It also reduced the lipopolysaccharide-stimulated release of tumor necrosis factor-alpha and intefleukin-1β from microglial cells. Paeonol possesses neuroprotective activity in a model of inflammation-induced neurotoxicity and reduces the release of neurotoxic and proinflammatory factors in activated microglial cells.展开更多
基金Supported by Natural Science Foundation of Fujian Province, No. c0410025
文摘Interleukin （IL）-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are possibly assodated with liver disease susceptibility or se-verity. Recombinant human IL-10 has been produced and is currently tested in clinical trials. These trials may give new insights into the immunobiology of IL-10 and suggest that the IL-10/IL-10 receptor system may become a new therapeutic target.
基金Fundationde AmparoàPesquisa do Estado de Minas Gerais,No.APQ-02320-18.
文摘BACKGROUND Chronic hepatitis C(CHC)is associated with type 2 diabetes mellitus.Although the pathogenesis remains to be elucidated,a growing evidence has suggested a role of pro-inflammatory immune response.Increased serum concentrations of Interleukin 6(IL-6)have been associated with insulin resistance,type 2 diabetes mellitus as well as advanced forms of liver disease in chronic hepatitis C infection.AIM To investigate the frequency of IL-6-174G/C(rs1800795)single nucleotide polymorphism(SNP)in CHC patients and in healthy subjects of the same ethnicity.Associations between type 2 diabetes mellitus(dependent variable)and demographic,clinical,nutritional,virological and,IL-6 genotyping data were also investigated in CHC patients.METHODS Two hundred and forty-five patients with CHC and 179 healthy control subjects(blood donors)were prospectively included.Type 2 diabetes mellitus was diagnosed according to the criteria of the American Diabetes Association.Clinical,biochemical,histological and radiological methods were used for the diagnosis of the liver disease.IL-6 polymorphism was evaluated by Taqman SNP genotyping assay.The data were analysed by logistic regression models.RESULTS Type 2 diabetes mellitus,blood hypertension and liver cirrhosis were observed in 20.8%(51/245),40.0%(98/245)and 38.4%(94/245)of the patients,respectively.The frequency of the studied IL-6 SNP did not differ between the CHC patients and controls(P=0.81)and all alleles were in Hardy-Weinberg equilibrium(P=0.38).In the multivariate analysis,type 2 diabetes mellitus was inversely associated with GC and CC genotypes of IL-6-174(OR=0.42;95%CI=0.22-0.78;P=0.006)and positively associated with blood hypertension(OR=5.56;95%CI=2.79-11.09;P<0.001).CONCLUSION This study was the first to show that GC and CC genotypes of IL-6-174 SNP are associated with a decreased risk of type 2 diabetes mellitus in patients chronically infected with hepatitis C virus.The identification of potential inflammatory mediators involved in the crosstalk between hepatitis C virus and the axis pancreas-liver remains important issues that deserve further investigations.
文摘目的研究慢性乙型肝炎病毒(Hepatitis B Virus,HBV)感染者血清甲状腺激素、抗甲状腺自身抗体及白细胞介素(interleukin,IL)-6水平变化及其临床意义。方法选取健康对照者18例、慢性乙型肝炎(CHB)患者65例和乙型肝炎肝硬化患者34例,应用电化学发光法分别检测并比较血清甲状腺激素水平、促甲状腺激素(TSH)、抗甲状腺相关抗体及IL-6水平。结果 CHB患者抗甲状腺过氧化物酶抗体(TPOAb)、抗甲状腺球蛋白抗体(TGAb)、抗促甲状腺激素受体抗体(TRAb)和IL-6水平分别为(9.5±5.6)KIU/L、(42.5±218.7)KIU/L、(1.5±0.7)IU/L和(6.6±12.6)pg/mL,在肝硬化患者分别为(9.5±8.6)KIU/L、(47.6±101.5)KIU/L、(2.2±1.7)IU/L和(15.8±25.5)pg/mL,均显著高于正常组[分别为(4.4±5.6)KIU/L、(7.7±18.3)KIU/L、(0.8±0.8)IU/L和(2.8±2.0)pg/mL,P<0.05],而CHB和肝硬化患者血FT3分别为(2.4±0.5)ng/L和(2.4±0.6)ng/L,均显著低于对照组[(2.9±0.2)ng/L,P<0.05];肝硬化患者TRAb和IL-6水平分别为(2.2±1.7)IU/L和(15.8±25.5)pg/mL,明显高于CHB患者[(1.5±0.7)IU/L和(6.6±12.6)pg/mL,P<0.05],而TT3和TT4水平分别为(0.8±0.3)nmol/L和(5.4±1.9)nmol/L,显著低于CHB患者[(1.3±0.3)nmol/L和(8.0±2.2)nmol/L,P<0.05]。结论联合检测慢性HBV感染者血清甲状腺激素、抗甲状腺自身抗体及IL-6水平对病情评价和预后判断有一定的临床价值。
文摘目的：检测膝骨关节炎（osteoarthritis，OA）患者血清及关节液中白细胞介素-17（in-terleukin-17）的表达并评估其与 OA 严重程度的相关性。方法收集100例原发性膝 OA 患者血清及关节液标本，并以50例健康志愿者血清标本作为对照。OA 严重程度及分级分别采用 Lequesne指数和 K-L 分级系统。酶联免疫吸附法分别检测血清及关节液中 IL-17浓度，并分析其与 OA 严重程度的相关性。结果OA 组患者血清中 IL-17浓度[（5．292±1．470）pg／ml]明显高于对照组[（4．173±0．640）pg／ml]，差异有统计学意义（P ＜0．05）。K-L4级或3级 OA 患者关节液 IL-17的浓度明显高于2级 OA 患者，差异有统计学意义（P ＜0．05）。4级 OA 患者关节液 IL-17的浓度[（6．161±1．120）pg／ml]明显高于3级 OA 患者[（4．474±0．816）pg／ml]，差异有统计学意义（P ＜0．05）。关节液 IL-17浓度与 Lequesne 指数呈正相关（r ＝0．6232，P ＜0．05）；血清 IL-17浓度与 Lequesne 指数之间无明显相关。结论IL-17在 OA 患者血清及关节液中的表达明显升高，关节液中 IL-17的水平与 OA 严重程度之间明显相关，提示 IL-17的失衡表达在 OA 的发病机制中具有一定作用。
文摘Chronic activation of microglial cells endangers neuronal survival through the release of various proinflammatory and neurotoxic factors. The root of Paeonia lactiflora Pall has been considered useful for the treatment of various disorders in traditional oriental medicine. Paeonol, found in the root of Paeonia lactiflora Pall, has a wide range of pharmacological functions, including anti-oxidative, anti-inflammatory and neuroprotective activities. The objective of this study was to examine the efficacy of paeonol in the repression of inflammation-induced neurotoxicity and microglial cell activation. Organotypic hippocampal slice cultures and primary microglial cells from rat brain were stimulated with bacterial lipopolysaccharide. Paeonol pretreatment was performed for 30 minutes prior to lipopolysaccharide addition. Cell viability and nitrite （the production of nitric oxide）, tumor necrosis factor-alpha and interleukin-lbeta products were measured after lipopolysaccharide treatment. In organotypic hippocampal slice cultures, paeonol blocked lipopolysaccharide-related hippocampal cell death and inhibited the release of nitrite and interleukin-lbeta. Paeonol was effective in inhibiting nitric oxide release from primary microglial cells. It also reduced the lipopolysaccharide-stimulated release of tumor necrosis factor-alpha and intefleukin-1β from microglial cells. Paeonol possesses neuroprotective activity in a model of inflammation-induced neurotoxicity and reduces the release of neurotoxic and proinflammatory factors in activated microglial cells.
