水热法合成了一个无机-有机杂化的NH_(4)[Cu_(3)^(I)(C_(10)H_(8)N_(2))_(3)Mo_(8)O_(26)]化合物,通过元素分析和单晶X-射线衍射进行了表征。化合物为三斜晶系,P1空间群,晶胞参数a=1.08763(9)nm,b=1.12674(10)nm,c=1.13067(10)nm,α=68....水热法合成了一个无机-有机杂化的NH_(4)[Cu_(3)^(I)(C_(10)H_(8)N_(2))_(3)Mo_(8)O_(26)]化合物,通过元素分析和单晶X-射线衍射进行了表征。化合物为三斜晶系,P1空间群,晶胞参数a=1.08763(9)nm,b=1.12674(10)nm,c=1.13067(10)nm,α=68.4820(10)°,β=83.523(2)°,γ=64.4180(10)°,V=1.16095(2)nm^(3),Z=1,Dc=2.661 g/cm^(3),Mr=1860.73,μ(MoKα)=35.22 cm^(-1),F(000)=888,R=0.0478,wR=0.099。化合物的结构包含2个结晶学上独立的铜原子、不连续的多氧阴离子β-[Mo_(8)O_(2)6]4-和无限扩展的[Cu I(C_(10)H_(8)N_(2))]链。每一个铜原子为类似的{CuN_(2)}配位模式,被4,4’-联吡啶连接成一维沿a轴方向的[Cu(C 10 H 8 N 2)]+链。分子结构中存在氢键和π…π作用。对化合物的热稳定性、荧光性质也进行了研究。展开更多
To investigate interleukin (IL)-26 expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and the function of IL-26. METHODSHuman colonic subepithelial myofibroblasts (SEMFs) were isolated...To investigate interleukin (IL)-26 expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and the function of IL-26. METHODSHuman colonic subepithelial myofibroblasts (SEMFs) were isolated from colon tissue surgically resected. The expression of IL-26 protein and its receptor complex was analyzed by immunohistochemistry. The gene expression induced by IL-26 was evaluated by real-time polymerase chain reaction. Intracellular signaling pathways were evaluated by immunoblotting and specific small interfering (si) RNA transfection. RESULTSThe mRNA and protein expression of IL-26 were significantly enhanced in the inflamed mucosa of patients with IBD. IL-26 receptor complex was expressed in colonic SEMFs in vivo and in vitro. IL-26 stimulated the mRNA expression of IL-6 and IL-8 in colonic SEMFs. The inhibitors of mitogen-activated protein kinases and phosphoinositide 3-kinase, and siRNAs for signal transducers and activator of transcription 1/3, nuclear factor-kappa B and activator protein-1 significantly reduced the mRNA expression of IL-6 and IL-8 induced by IL-26. CONCLUSIONThese results suggest that IL-26 plays a role in the pathophysiology of IBD through induction of inflammatory mediators.展开更多
AIM: To detect the possible role of interleukin(IL)-26 in diabetic retinopathy(DR) patients.METHODS: Subjects were divided into diabetes without retinopathy(DWR) group(n=20), non-proliferative diabetic retinopathy(NPD...AIM: To detect the possible role of interleukin(IL)-26 in diabetic retinopathy(DR) patients.METHODS: Subjects were divided into diabetes without retinopathy(DWR) group(n=20), non-proliferative diabetic retinopathy(NPDR) group(n=20), proliferative diabetic retinopathy(PDR) group(n=20) and normal control group(n=20). The protein expression of IL-26 in the serum and vitreous fluid were measured by enzyme-linked immunosorbent assay(ELISA). The m RNA change of IL-26 in peripheral blood mononuclear cells(PBMCs) was assessed by real-time polymerase chain reaction.RESULTS: The serum expression of IL-26 in PDR group was significantly elevated compared with the normal control group, DWR group and NPDR group. The vitreous fluid concentration of IL-26 in PDR patients(without antiVEGF therapy) was also higher compared to normal controls. However, no obvious significance was found concerning the expression of IL-26 in vitreous fluid between PDR after anti-VEGF therapy and normal controls. In PDR group, the m RNA level of IL-26 significantly increased compared with the normal controls and DWR patients in the PBMCs.CONCLUSION: Protein and m RNA expression of IL-26 are increased in serum, vitreous fluid and PBMCs in PDR patients, suggesting that IL-26 may be associated with the pathogenesis of PDR.展开更多
文摘水热法合成了一个无机-有机杂化的NH_(4)[Cu_(3)^(I)(C_(10)H_(8)N_(2))_(3)Mo_(8)O_(26)]化合物,通过元素分析和单晶X-射线衍射进行了表征。化合物为三斜晶系,P1空间群,晶胞参数a=1.08763(9)nm,b=1.12674(10)nm,c=1.13067(10)nm,α=68.4820(10)°,β=83.523(2)°,γ=64.4180(10)°,V=1.16095(2)nm^(3),Z=1,Dc=2.661 g/cm^(3),Mr=1860.73,μ(MoKα)=35.22 cm^(-1),F(000)=888,R=0.0478,wR=0.099。化合物的结构包含2个结晶学上独立的铜原子、不连续的多氧阴离子β-[Mo_(8)O_(2)6]4-和无限扩展的[Cu I(C_(10)H_(8)N_(2))]链。每一个铜原子为类似的{CuN_(2)}配位模式,被4,4’-联吡啶连接成一维沿a轴方向的[Cu(C 10 H 8 N 2)]+链。分子结构中存在氢键和π…π作用。对化合物的热稳定性、荧光性质也进行了研究。
文摘To investigate interleukin (IL)-26 expression in the inflamed mucosa of patients with inflammatory bowel disease (IBD) and the function of IL-26. METHODSHuman colonic subepithelial myofibroblasts (SEMFs) were isolated from colon tissue surgically resected. The expression of IL-26 protein and its receptor complex was analyzed by immunohistochemistry. The gene expression induced by IL-26 was evaluated by real-time polymerase chain reaction. Intracellular signaling pathways were evaluated by immunoblotting and specific small interfering (si) RNA transfection. RESULTSThe mRNA and protein expression of IL-26 were significantly enhanced in the inflamed mucosa of patients with IBD. IL-26 receptor complex was expressed in colonic SEMFs in vivo and in vitro. IL-26 stimulated the mRNA expression of IL-6 and IL-8 in colonic SEMFs. The inhibitors of mitogen-activated protein kinases and phosphoinositide 3-kinase, and siRNAs for signal transducers and activator of transcription 1/3, nuclear factor-kappa B and activator protein-1 significantly reduced the mRNA expression of IL-6 and IL-8 induced by IL-26. CONCLUSIONThese results suggest that IL-26 plays a role in the pathophysiology of IBD through induction of inflammatory mediators.
基金Supported by the National Natural Science Foundation of China (No.81870643)
文摘AIM: To detect the possible role of interleukin(IL)-26 in diabetic retinopathy(DR) patients.METHODS: Subjects were divided into diabetes without retinopathy(DWR) group(n=20), non-proliferative diabetic retinopathy(NPDR) group(n=20), proliferative diabetic retinopathy(PDR) group(n=20) and normal control group(n=20). The protein expression of IL-26 in the serum and vitreous fluid were measured by enzyme-linked immunosorbent assay(ELISA). The m RNA change of IL-26 in peripheral blood mononuclear cells(PBMCs) was assessed by real-time polymerase chain reaction.RESULTS: The serum expression of IL-26 in PDR group was significantly elevated compared with the normal control group, DWR group and NPDR group. The vitreous fluid concentration of IL-26 in PDR patients(without antiVEGF therapy) was also higher compared to normal controls. However, no obvious significance was found concerning the expression of IL-26 in vitreous fluid between PDR after anti-VEGF therapy and normal controls. In PDR group, the m RNA level of IL-26 significantly increased compared with the normal controls and DWR patients in the PBMCs.CONCLUSION: Protein and m RNA expression of IL-26 are increased in serum, vitreous fluid and PBMCs in PDR patients, suggesting that IL-26 may be associated with the pathogenesis of PDR.
