Background: Tuberculosis (TB) is one of the world’s deadliest infectious diseases. Tumor necrosis factor-Alpha (TNF-α) and Interleukin 8 (IL-8) are involved in the pathogenesis of pulmonary TB (PTB). However, the co...Background: Tuberculosis (TB) is one of the world’s deadliest infectious diseases. Tumor necrosis factor-Alpha (TNF-α) and Interleukin 8 (IL-8) are involved in the pathogenesis of pulmonary TB (PTB). However, the contribution of polymorphisms of these cytokines to PTB susceptibility needed more investigation across geographic regions and ethnic groups. Purpose: The aim of this study was to investigate the association of the TNF-α-308 G/A and IL-8-251T/A polymorphisms with PTB risk in the Congolese population. Methods: This case-control study included 150 PTB patients and 160 control subjects. Blood samples were collected from all participants and were used for the TNF-α-308 G/A and IL-8-251T/A genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Odds ratios (OR) were calculated to estimate the potential polymorphism associations. A P level of Results: A significant difference was found between PTB patients and controls regarding the TNF-α-308AA genotype (P = 0.035) distribution. Moreover, this genotype was associated with risk to TB (OR = 7.19, 95% CI = 0.85 - 60.65, P = 0.035). The A allele was significantly more frequent in PTB patients than in controls, and was associated with risk to PTB (OR = 1.68, 95% CI = 1.05 - 2.68, P = 0.014). Regarding the IL-8-251T/A gene, TA and AA genotypes were significantly more frequent in PTB patients compared to controls, and were associated with increased risk to PTB (OR = 2.64, 95% CI = 0.97 - 7.18, P = 0.031 and OR = 3.0, 95% CI = 1.13 - 7.98, P = 0.014, respectively). However, the IL-8-251 A allele was not associated to PTB susceptibility (OR = 0.27, 95% CI = 0.15 - 0.44). Conclusion: TNF-α-308G/A and IL-8-251T/A polymorphisms may be associated to PTB susceptibility in the Congolese population, and the AA genotype of both cytokines could be a risk factor.展开更多
Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leish...Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.展开更多
文摘Background: Tuberculosis (TB) is one of the world’s deadliest infectious diseases. Tumor necrosis factor-Alpha (TNF-α) and Interleukin 8 (IL-8) are involved in the pathogenesis of pulmonary TB (PTB). However, the contribution of polymorphisms of these cytokines to PTB susceptibility needed more investigation across geographic regions and ethnic groups. Purpose: The aim of this study was to investigate the association of the TNF-α-308 G/A and IL-8-251T/A polymorphisms with PTB risk in the Congolese population. Methods: This case-control study included 150 PTB patients and 160 control subjects. Blood samples were collected from all participants and were used for the TNF-α-308 G/A and IL-8-251T/A genotyping by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Odds ratios (OR) were calculated to estimate the potential polymorphism associations. A P level of Results: A significant difference was found between PTB patients and controls regarding the TNF-α-308AA genotype (P = 0.035) distribution. Moreover, this genotype was associated with risk to TB (OR = 7.19, 95% CI = 0.85 - 60.65, P = 0.035). The A allele was significantly more frequent in PTB patients than in controls, and was associated with risk to PTB (OR = 1.68, 95% CI = 1.05 - 2.68, P = 0.014). Regarding the IL-8-251T/A gene, TA and AA genotypes were significantly more frequent in PTB patients compared to controls, and were associated with increased risk to PTB (OR = 2.64, 95% CI = 0.97 - 7.18, P = 0.031 and OR = 3.0, 95% CI = 1.13 - 7.98, P = 0.014, respectively). However, the IL-8-251 A allele was not associated to PTB susceptibility (OR = 0.27, 95% CI = 0.15 - 0.44). Conclusion: TNF-α-308G/A and IL-8-251T/A polymorphisms may be associated to PTB susceptibility in the Congolese population, and the AA genotype of both cytokines could be a risk factor.
基金supported by a grant from the Tabriz University of Medical Sciences(No.91-20)
文摘Objective:To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients.Methods:Three groups including patients with VL clinical presentation and leishmania seropositive(n=124).patients seropositive but without clinical presentation(n=82) and healthy controls(n=63) were selected to conduct this cross-sectional study.Polymorphism at +2767 position of IL-8 was investigated using PCR-RPLP techniques.Anti-leishmania antibody titration was evaluated by the immunoflorescence technique.Results:We observed higher significant frequencies +2767 A/A and A/T genotypes in groups I compared to group 2 and healthy controls(P=0.001).Also,patients in Group 1 carriyng A/A genotype showed higher liter of antileshmania antibody than patients with A/T and T/T genotypes(P=0.05).The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance(ANOVA),as well as x^2tests.Conclusions:Our findings indicate that the IL-8 +2767 polymorphism is significantly involved in impaired immune responses against VL and it could be considered as a risk factor for the VL progress.
