期刊文献+
共找到1篇文章
< 1 >
每页显示 20 50 100
Inhibitory Effects of Blockage of Intermediate Conductance Ca^(2+) -Activated K^+ Channels on Proliferation of Hepatocellular Carcinoma Cells 被引量:5
1
作者 杨筱嵬 刘谨文 +3 位作者 张汝超 殷 茜 沈文状 易继林 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第1期86-89,共4页
The roles of intermediate conductance Ca2+-activated K+ channel (IKCal) in the pathogene- sis of hepatocellular carcinoma (HCC) were investigated. Immunohistochemistry and Western blotting were used to detect th... The roles of intermediate conductance Ca2+-activated K+ channel (IKCal) in the pathogene- sis of hepatocellular carcinoma (HCC) were investigated. Immunohistochemistry and Western blotting were used to detect the expression of IKCal protein in 50 HCC and 20 para-carcinoma tissue samples. Real-time PCR was used to detect the transcription level of IKCal mRNA in 13 HCC and 11 para-carcinoma tissue samples. The MTT assay was used to measure the function of IKCal in human HCC cell line HepG2 in vitro. TRAM-34, a specific blocker of IKCal, was used to intervene with the function of IKCal. As compared with para-carcinoma tissue, an over-expression of IKCal protein was detected in HCC tissue samples (P〈0.05). The mRNA expression level of IKCal in HCC tissues was 2.17 times higher than that in para-carcinoma tissues. The proliferation of HepG2 cells was suppressed by TRAM-34 (0.5, 1.0, 2.0 and 4.0 pxnol/L) in vitro (P〈0.05). Our results suggested that IKCal may play a role in the proliferation of human HCC, and IKCal blockers may represent a potential therapeutic strategy for HCC. 展开更多
关键词 intermediate conductance Ca2+-activated K+ channel hepatocellular carcinoma TRAM-34 PROLIFERATION
下载PDF
上一页 1 下一页 到第
使用帮助 返回顶部