In the present study,municipal secondary sludge and purified glycerol(obtained after acid treatment of crude glycerol)were used together for lipid production using intermittent feeding strategy.Intermittent sludge fee...In the present study,municipal secondary sludge and purified glycerol(obtained after acid treatment of crude glycerol)were used together for lipid production using intermittent feeding strategy.Intermittent sludge feeding strategy(sludge SS 30 g/L)resulted in a higher biomass(54.99 g/L)and lipid concentration(25.35 g/L)at 96 h when compared to 35 g/L SS single sludge feeding or control strategy(45.67 g/L biomass&19.16 g/L lipid).Moreover,the intermittent sludge feeding strategy significantly reduced foaming and requirement of anti-foam during fermentation when compared to control strategy.The energy balance of biodiesel production from lipid obtained by intermittent sludge feeding strategy(30 g/L SS)was energeti-cally favorable.It was also revealed from yield coefficients and energy balance that sludge had an important contribution in microbial lipid and biodiesel production.展开更多
文摘目的探讨脑卒中吞咽障碍行间歇经口至食管管饲法(intermittent oro-esophageal tube feeding,IOE)发生误吸的风险评估及模型构建。方法选取2020年1月至2022年12月温州医科大学附属第二医院就诊的脑卒中吞咽障碍行IOE的146例患者为研究对象,根据IOE后是否误吸将其分为未误吸组(117例)和误吸组(29例),收集患者的临床资料,采用多因素Logistic回归分析患者发生误吸的危险因素,并建立列线图预测模型,利用C指数进行区分度评价,校准曲线进行列线图拟合度检测。结果两组患者的脑卒中病史、误吸史、脑卒中部位、自主咳嗽、洼田饮水试验、美国国立卫生研究院卒中量表(National Institutes of Health stroke scale,NIHSS)评分、置管体位、置管深度比较差异均有统计学意义(P<0.05);多因素Logistic回归分析结果显示,有脑卒中病史、有误吸史、脑卒中部位为脑干、洼田饮水试验4~5级、NIHSS评分高、侧卧位置管、置管深度低均是脑卒中吞咽障碍行IOE患者误吸的危险因素(P<0.05),自主咳嗽是脑卒中吞咽障碍行IOE患者误吸的保护因素(P<0.05);列线图预测模型判断误吸风险的C指数为0.705(95%CI:0.591~0.857),Hosmer-Lemeshow检验模型的拟合度良好;受试者操作特征曲线结果显示列线图预测模型的曲线下面积为0.756,敏感度为75.42%,特异性为77.09%。结论脑卒中病史、误吸史、脑卒中部位、自主咳嗽、洼田饮水试验、NIHSS评分、置管体位、置管深度均是脑卒中吞咽障碍行IOE患者误吸的影响因素,模型构建成功,构建的模型有良好的区分度和拟合度。
基金This research has been funded by the Natural Sciences and Engineering Research Council of Canada(Grant A4984).
文摘In the present study,municipal secondary sludge and purified glycerol(obtained after acid treatment of crude glycerol)were used together for lipid production using intermittent feeding strategy.Intermittent sludge feeding strategy(sludge SS 30 g/L)resulted in a higher biomass(54.99 g/L)and lipid concentration(25.35 g/L)at 96 h when compared to 35 g/L SS single sludge feeding or control strategy(45.67 g/L biomass&19.16 g/L lipid).Moreover,the intermittent sludge feeding strategy significantly reduced foaming and requirement of anti-foam during fermentation when compared to control strategy.The energy balance of biodiesel production from lipid obtained by intermittent sludge feeding strategy(30 g/L SS)was energeti-cally favorable.It was also revealed from yield coefficients and energy balance that sludge had an important contribution in microbial lipid and biodiesel production.
