Coherent beam combining(CBC) of fiber laser array is a promising technique to realize high output power while maintaining near diffraction-limited beam quality. To implement CBC, an appropriate phase control feedback ...Coherent beam combining(CBC) of fiber laser array is a promising technique to realize high output power while maintaining near diffraction-limited beam quality. To implement CBC, an appropriate phase control feedback structure should be established to realize phase-locking. In this paper, an innovative internal active phase control CBC fiber laser array based on photodetector array is proposed. The dynamic phase noises of the laser amplifiers are compensated before being emitted into free space. And the static phase difference compensation of emitting laser array is realized by interference measurement based on photodetector array. The principle of the technique is illustrated and corresponding simulations are carried out, and a CBC system with four laser channels is built to verify the technique. When the phase controllers are turned on, the phase deviation of the laser array is less than λ/20, and ~ 95% fringe contrast of the irradiation distribution is obtained. The technique proposed in this paper could provide a reference for the system design of a massive high-power CBC system.展开更多
This paper outlines the internal control management of hospital accounting and finance,introducing its concept,importance,and objectives.It then analyzes the problems existing in the internal control management of hos...This paper outlines the internal control management of hospital accounting and finance,introducing its concept,importance,and objectives.It then analyzes the problems existing in the internal control management of hospital accounting and finance,including an unsound management system,insufficient system implementation,inadequate risk assessment and control capabilities,poor information communication,weak internal supervision,and uneven personnel quality.The reasons for these problems are also analyzed,including outdated management concepts,irrational organizational structures,imperfect training and incentive mechanisms,low levels of information technology,and changes in the external environment.This paper proposes improvement measures for the internal control management of hospital accounting,including enhancing the management system,strengthening system implementation,improving risk assessment and control capabilities,enhancing the information communication mechanism,strengthening internal supervision,improving personnel quality,advancing information technology,and adapting to changes in the external environment.展开更多
Small and medium-sized enterprises(SMEs)constitute the primary drivers of production within the nation’s existing enterprise landscape.They represent the most dynamic segment of the national economy and play a pivota...Small and medium-sized enterprises(SMEs)constitute the primary drivers of production within the nation’s existing enterprise landscape.They represent the most dynamic segment of the national economy and play a pivotal role in supporting economic growth,fostering employment,and enhancing people’s livelihoods.However,despite their significant and extensive organizational structures,only a fraction of these companies have established internal control systems,and even fewer possess robust ones.Building upon this premise and considering the prevailing circumstances of SMEs,this paper undertakes an empirical analysis of monetary fund management within this sector.It delves into the operational intricacies,exploring how monetary funds should be effectively implemented to ensure their safety and integrity.Furthermore,it proposes viable strategies to enhance the circulation efficiency of monetary funds,thereby maximizing benefits for enterprises.Addressing these challenges is crucial for SME managers striving to achieve sustainable profit growth and navigate the complexities of financial management.展开更多
As an important pillar of national economic development,state-owned enterprises,their operational efficiency,and risk management ability are directly related to the stability and security of the national economy.As an...As an important pillar of national economic development,state-owned enterprises,their operational efficiency,and risk management ability are directly related to the stability and security of the national economy.As an important part of enterprise management,internal control management plays an irreplaceable role.Especially in the current domestic and international economic situation is complex and changeable,market competition is increasingly fierce environment,to strengthen the internal control management of state-owned enterprises and risk prevention measures is particularly important.This paper starts with the importance of internal control management and risk prevention for state-owned enterprises,and analyzes the problems and strategies in the internal control management and risk prevention of state-owned enterprises,in order to build a more comprehensive and efficient risk management system for state-owned enterprises to adapt to the ever-changing market environment and realize sustainable development.展开更多
Today the high quality power supply is of essential in the economic development in a country. With the development of modem power systems and increasing demand for power supply, the electric power industry is facing a...Today the high quality power supply is of essential in the economic development in a country. With the development of modem power systems and increasing demand for power supply, the electric power industry is facing a great challenge in meeting the increased load demand with highest reliability and security with minimum transmission expenditure. Power system stability analysis and control is one of the most important issues in power systems. The problem becomes more and more serious in power systems with the development of modem power systems. The ability of a power system展开更多
The 6th Intemational Conference on Control and Automation(ICCA'07)will be held on May 30 to June 1,2007,in Guangzhou,China.The conference is jointly organized by the IEEE Control Systems Chapter,Singapore and IEEE...The 6th Intemational Conference on Control and Automation(ICCA'07)will be held on May 30 to June 1,2007,in Guangzhou,China.The conference is jointly organized by the IEEE Control Systems Chapter,Singapore and IEEE Control Systems Chapter,Guangzhou,China,and spon- sored by South China University of Technology,China and Chinese University of Hong Kong, Hong Kong.It is to create a forum for scientists and practicing engineers throughout the world to present the latest research findings and ideas in the areas of control and automation.展开更多
Conference Theme-Anti-Crime Networking and Systems The 2007 IEEE International Conference on Networking,Sensing and Control will be held in London. The main theme of the conference is anti-crime networking and critica...Conference Theme-Anti-Crime Networking and Systems The 2007 IEEE International Conference on Networking,Sensing and Control will be held in London. The main theme of the conference is anti-crime networking and critical infrastructure.The area of anti- crime networking and critical infrastructure is a fusion of a number of research areas in networking, sensing,human factors,artificial intelligence,operational research,and systems control theory. However,the real challenge is to design anti-crime networking and systems from a holistic perspective; taking into account technical,organizational as well as contextual complexity.A system engineering approach is required to address new problems of this challenging and promising area.This conference will provide a remarkable opportunity for the academic and industrial community to address new challenges and share solutions,and discuss future research directions.It will feature plenary speeches, industrial panel sessions,funding agency panel sessions,interactive sessions,and invited/special sessions.Contributions are expected from academia,industry,EPSRC,EU,DTI,and MoD.Technical topics of the conference include but are not limited to:展开更多
Date: April 12-15, 2005 Venue: Zhejiang University, Hangzhou, P. R. China Organized by Institute of Mechatronic Control Engineering, Zhejiang University
In this paper,an optimized Genetic Algorithm(GA)based internal model controller-proportional integral derivative(IMC-PID)controller has been designed for the control variable to output variable transfer function of dc...In this paper,an optimized Genetic Algorithm(GA)based internal model controller-proportional integral derivative(IMC-PID)controller has been designed for the control variable to output variable transfer function of dc-dc boost converter to mitigate the effect of non-minimum phase(NMP)behavior due to the presence of a right-half plane zero(RHPZ).This RHPZ limits the dynamic performance of the converter and leads to internal instability.The IMC PID is a streamlined counterpart of the standard feedback controller and easily achieves optimal set point and load change performance with a single filter tuning parameterλ.Also,this paper addresses the influences of the model-based controller with model plant mismatch on the closed-loop control.The conventional IMC PID design is realized as an optimization problem with a resilient controller being determined through a genetic algorithm.Computed results suggested that GA–IMC PID coheres to the optimum designs with a fast convergence rate and outperforms conventional IMC PID controllers.展开更多
About one year on,international arms control and disarmament appeared to be rebounding to become an issue of great international attention,paralleling the issues of financial crisis and climate change.International nu...About one year on,international arms control and disarmament appeared to be rebounding to become an issue of great international attention,paralleling the issues of financial crisis and climate change.International nuclear disarmament re-dawned with high profile readjustments by the United States in its nuclear arms control policy and tactics.The US and Russia signed a new agreement of bilateral nuclear disarmament.Multilateral nuclear arms control has also softened,and the nuclear security issue loomed large.The Conference on the Review of Nuclear Non-proliferation was held,the main illustration of the adjustment by the US to its arms control policy and tactics.On balance,however,the US arms control and disarmament policies remain largely rhetorical,as its two strategic objectives of nuclear weapon policy--nuclear deterrence and non-proliferation--has been kept intact,and even intensified.展开更多
Strategic management oriented enterprise internal control is an inevitable choice after the enterprise develops to a certain scale,and it is also an important starting point for enterprises to adjust their own strateg...Strategic management oriented enterprise internal control is an inevitable choice after the enterprise develops to a certain scale,and it is also an important starting point for enterprises to adjust their own strategic layout and resource allocation.With the continuous expansion of the scope of internal control supervision,the traditional mode of internal control supervision has become more and more restricted.In view of this,the present study started from the necessity of internal control supervision from the overall and top-level perspectives,and analyzed the current problems in the internal control of enterprises from the perspective of strategic management.The problems include the lack of methods and tools of strategic management,insufficient awareness of strategic risk management,insufficient expansion of internal control objects,insufficient prior intervention in internal control supervision,etc.Finally,it came up with the idea of constructing strategic enterprise internal control,in order to provide an effective reference for the study of enterprise internal control.展开更多
Focusing on the two themes of"internal control"and"risk management",this paper makes an in-depth analysis of the current situation of risk management and internal control of Moutai Group.It analyze...Focusing on the two themes of"internal control"and"risk management",this paper makes an in-depth analysis of the current situation of risk management and internal control of Moutai Group.It analyzes the current situation of risk management and internal control of Moutai Group.It is found that the risk management of Moutai Group is not perfect,the information exchange is not smooth,and the internal control assessment is not perfect.Finally,it puts forward some corresponding countermeasures,including establishing an effective information communication mechanism,perfecting the risk assessment system,and strengthening the construction of the professional talent team.展开更多
A modified two-degrees-of-freedom( M-TDOF) internal model control( IMC) method is proposed for non-square systems with multiple time delays and right-half-plane( RHP) zeros. In this method,pseudo-inverse is introduced...A modified two-degrees-of-freedom( M-TDOF) internal model control( IMC) method is proposed for non-square systems with multiple time delays and right-half-plane( RHP) zeros. In this method,pseudo-inverse is introduced to design the internal model controller,and a desired closed-loop transfer function is designed to eliminate the unrealizable factors of the derived controller. In addition,set-point tracking and load-disturbance rejection of each process are separately controlled by two controllers. The simulation results show that in addition to high decoupling performance and robustness,the proposed control method also effectively improves loaddisturbance rejection and simultaneously optimizes the input tracking performance and disturbance rejection performance by selecting the parameters of controllers. Furthermore,the higher tolerance of model mismatch is achieved in this paper.展开更多
Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these char...Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these characteristics, gene expression has been investigated at the whole transcriptome level. Gene expression studies using quantitative real-time PCR should start by selecting an appropriate internal control gene; therefore, the absolute expression abundance of six housekeeping genes (18S rRNA (18S), ubiquitin-conju-ating enzyme (UBC), actin (ACT), β-tubulin (TUB), elongation factors 2 (EF2), and glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) examined by the quantitative real-time PCR in samples corresponding to different strains, life-cycle stages and abiotic stress treatments. Their expression stabilities were assessed by the comparative cycle threshold (Ct) method and by two different software packages: geNorm and NormFinder. The most stable housekeeping gene is UBC and the least stable housekeeping is GADPH. Thus, it is proposed that the most appropriate internal control gene for expression analyses in P. haitanensis is UBC. The results pave the way for further gene expression analyses of different aspects of P. haitanensis biology including different strains, life-history stages and abiotic stress responses.展开更多
There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In ...There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.展开更多
A novel control scheme of active disturbance rejection internal model control(ADRIMC) is proposed to improve the anti-interference ability and robustness for the dead-time process. The active anti-interference concept...A novel control scheme of active disturbance rejection internal model control(ADRIMC) is proposed to improve the anti-interference ability and robustness for the dead-time process. The active anti-interference concept is introduced into the internal model control(IMC) by analyzing the relationship between IMC and disturbance observer control(DOB). Further, a design process of disturbance filter is presented to realize the active anti-interference ability for ADRIMC scheme. The disturbance filter is used to estimate an equivalent disturbance consisting of both external disturbances and internal disturbances caused by model mismatches.Simulation results demonstrate that the proposed method possesses a good disturbance rejection performance, though losing some partial dynamic performance. In other words, the proposed method shows a tradeoff between the dynamic performance and the system robust.展开更多
Because of vehicle's external disturbances and model uncertainties,robust control algorithms have obtained popularity in vehicle stability control.The robust control usually gives up performance in order to guarantee...Because of vehicle's external disturbances and model uncertainties,robust control algorithms have obtained popularity in vehicle stability control.The robust control usually gives up performance in order to guarantee the robustness of the control algorithm,therefore an improved robust internal model control(IMC) algorithm blending model tracking and internal model control is put forward for active steering system in order to reach high performance of yaw rate tracking with certain robustness.The proposed algorithm inherits the good model tracking ability of the IMC control and guarantees robustness to model uncertainties.In order to separate the design process of model tracking from the robustness design process,the improved 2 degree of freedom(DOF) robust internal model controller structure is given from the standard Youla parameterization.Simulations of double lane change maneuver and those of crosswind disturbances are conducted for evaluating the robust control algorithm,on the basis of a nonlinear vehicle simulation model with a magic tyre model.Results show that the established 2-DOF robust IMC method has better model tracking ability and a guaranteed level of robustness and robust performance,which can enhance the vehicle stability and handling,regardless of variations of the vehicle model parameters and the external crosswind interferences.Contradiction between performance and robustness of active steering control algorithm is solved and higher control performance with certain robustness to model uncertainties is obtained.展开更多
An adaptive internal mode control is proposed to eliminate effectively periodic disturbance with uncertain frequency caused by input error angle of PIGA (Pendulous Integrating Gyro Accelerometer). An adaptive algori...An adaptive internal mode control is proposed to eliminate effectively periodic disturbance with uncertain frequency caused by input error angle of PIGA (Pendulous Integrating Gyro Accelerometer). An adaptive algorithm with periodic disturbance frequency identification on line is applied and the internal model controller parameters are adjusted to eliminate disturbance. Then the convergence of this algorithm and the stability of the system are proved by the averaging method. Simulation results verify the proposed scheme can eliminate periodic disturbance and improve the test precision for PIGA effectively.展开更多
PID controllers were used for the hydraulic servo system of sliding gate and the tundish weight control system in continuous caster.These two loops were synthesized in mould level controller based on model reduction a...PID controllers were used for the hydraulic servo system of sliding gate and the tundish weight control system in continuous caster.These two loops were synthesized in mould level controller based on model reduction and internal model control strategy.Satisfactory control performance of this synthetic mould level controller was demonstrated by simulations and on-line experiments.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant No.62275272)the Training Program for Excellent Young Innovators of Changsha(Grant No.KQ2305025)。
文摘Coherent beam combining(CBC) of fiber laser array is a promising technique to realize high output power while maintaining near diffraction-limited beam quality. To implement CBC, an appropriate phase control feedback structure should be established to realize phase-locking. In this paper, an innovative internal active phase control CBC fiber laser array based on photodetector array is proposed. The dynamic phase noises of the laser amplifiers are compensated before being emitted into free space. And the static phase difference compensation of emitting laser array is realized by interference measurement based on photodetector array. The principle of the technique is illustrated and corresponding simulations are carried out, and a CBC system with four laser channels is built to verify the technique. When the phase controllers are turned on, the phase deviation of the laser array is less than λ/20, and ~ 95% fringe contrast of the irradiation distribution is obtained. The technique proposed in this paper could provide a reference for the system design of a massive high-power CBC system.
文摘This paper outlines the internal control management of hospital accounting and finance,introducing its concept,importance,and objectives.It then analyzes the problems existing in the internal control management of hospital accounting and finance,including an unsound management system,insufficient system implementation,inadequate risk assessment and control capabilities,poor information communication,weak internal supervision,and uneven personnel quality.The reasons for these problems are also analyzed,including outdated management concepts,irrational organizational structures,imperfect training and incentive mechanisms,low levels of information technology,and changes in the external environment.This paper proposes improvement measures for the internal control management of hospital accounting,including enhancing the management system,strengthening system implementation,improving risk assessment and control capabilities,enhancing the information communication mechanism,strengthening internal supervision,improving personnel quality,advancing information technology,and adapting to changes in the external environment.
文摘Small and medium-sized enterprises(SMEs)constitute the primary drivers of production within the nation’s existing enterprise landscape.They represent the most dynamic segment of the national economy and play a pivotal role in supporting economic growth,fostering employment,and enhancing people’s livelihoods.However,despite their significant and extensive organizational structures,only a fraction of these companies have established internal control systems,and even fewer possess robust ones.Building upon this premise and considering the prevailing circumstances of SMEs,this paper undertakes an empirical analysis of monetary fund management within this sector.It delves into the operational intricacies,exploring how monetary funds should be effectively implemented to ensure their safety and integrity.Furthermore,it proposes viable strategies to enhance the circulation efficiency of monetary funds,thereby maximizing benefits for enterprises.Addressing these challenges is crucial for SME managers striving to achieve sustainable profit growth and navigate the complexities of financial management.
文摘As an important pillar of national economic development,state-owned enterprises,their operational efficiency,and risk management ability are directly related to the stability and security of the national economy.As an important part of enterprise management,internal control management plays an irreplaceable role.Especially in the current domestic and international economic situation is complex and changeable,market competition is increasingly fierce environment,to strengthen the internal control management of state-owned enterprises and risk prevention measures is particularly important.This paper starts with the importance of internal control management and risk prevention for state-owned enterprises,and analyzes the problems and strategies in the internal control management and risk prevention of state-owned enterprises,in order to build a more comprehensive and efficient risk management system for state-owned enterprises to adapt to the ever-changing market environment and realize sustainable development.
文摘Today the high quality power supply is of essential in the economic development in a country. With the development of modem power systems and increasing demand for power supply, the electric power industry is facing a great challenge in meeting the increased load demand with highest reliability and security with minimum transmission expenditure. Power system stability analysis and control is one of the most important issues in power systems. The problem becomes more and more serious in power systems with the development of modem power systems. The ability of a power system
文摘The 6th Intemational Conference on Control and Automation(ICCA'07)will be held on May 30 to June 1,2007,in Guangzhou,China.The conference is jointly organized by the IEEE Control Systems Chapter,Singapore and IEEE Control Systems Chapter,Guangzhou,China,and spon- sored by South China University of Technology,China and Chinese University of Hong Kong, Hong Kong.It is to create a forum for scientists and practicing engineers throughout the world to present the latest research findings and ideas in the areas of control and automation.
文摘Conference Theme-Anti-Crime Networking and Systems The 2007 IEEE International Conference on Networking,Sensing and Control will be held in London. The main theme of the conference is anti-crime networking and critical infrastructure.The area of anti- crime networking and critical infrastructure is a fusion of a number of research areas in networking, sensing,human factors,artificial intelligence,operational research,and systems control theory. However,the real challenge is to design anti-crime networking and systems from a holistic perspective; taking into account technical,organizational as well as contextual complexity.A system engineering approach is required to address new problems of this challenging and promising area.This conference will provide a remarkable opportunity for the academic and industrial community to address new challenges and share solutions,and discuss future research directions.It will feature plenary speeches, industrial panel sessions,funding agency panel sessions,interactive sessions,and invited/special sessions.Contributions are expected from academia,industry,EPSRC,EU,DTI,and MoD.Technical topics of the conference include but are not limited to:
文摘Date: April 12-15, 2005 Venue: Zhejiang University, Hangzhou, P. R. China Organized by Institute of Mechatronic Control Engineering, Zhejiang University
文摘In this paper,an optimized Genetic Algorithm(GA)based internal model controller-proportional integral derivative(IMC-PID)controller has been designed for the control variable to output variable transfer function of dc-dc boost converter to mitigate the effect of non-minimum phase(NMP)behavior due to the presence of a right-half plane zero(RHPZ).This RHPZ limits the dynamic performance of the converter and leads to internal instability.The IMC PID is a streamlined counterpart of the standard feedback controller and easily achieves optimal set point and load change performance with a single filter tuning parameterλ.Also,this paper addresses the influences of the model-based controller with model plant mismatch on the closed-loop control.The conventional IMC PID design is realized as an optimization problem with a resilient controller being determined through a genetic algorithm.Computed results suggested that GA–IMC PID coheres to the optimum designs with a fast convergence rate and outperforms conventional IMC PID controllers.
文摘About one year on,international arms control and disarmament appeared to be rebounding to become an issue of great international attention,paralleling the issues of financial crisis and climate change.International nuclear disarmament re-dawned with high profile readjustments by the United States in its nuclear arms control policy and tactics.The US and Russia signed a new agreement of bilateral nuclear disarmament.Multilateral nuclear arms control has also softened,and the nuclear security issue loomed large.The Conference on the Review of Nuclear Non-proliferation was held,the main illustration of the adjustment by the US to its arms control policy and tactics.On balance,however,the US arms control and disarmament policies remain largely rhetorical,as its two strategic objectives of nuclear weapon policy--nuclear deterrence and non-proliferation--has been kept intact,and even intensified.
文摘Strategic management oriented enterprise internal control is an inevitable choice after the enterprise develops to a certain scale,and it is also an important starting point for enterprises to adjust their own strategic layout and resource allocation.With the continuous expansion of the scope of internal control supervision,the traditional mode of internal control supervision has become more and more restricted.In view of this,the present study started from the necessity of internal control supervision from the overall and top-level perspectives,and analyzed the current problems in the internal control of enterprises from the perspective of strategic management.The problems include the lack of methods and tools of strategic management,insufficient awareness of strategic risk management,insufficient expansion of internal control objects,insufficient prior intervention in internal control supervision,etc.Finally,it came up with the idea of constructing strategic enterprise internal control,in order to provide an effective reference for the study of enterprise internal control.
文摘Focusing on the two themes of"internal control"and"risk management",this paper makes an in-depth analysis of the current situation of risk management and internal control of Moutai Group.It analyzes the current situation of risk management and internal control of Moutai Group.It is found that the risk management of Moutai Group is not perfect,the information exchange is not smooth,and the internal control assessment is not perfect.Finally,it puts forward some corresponding countermeasures,including establishing an effective information communication mechanism,perfecting the risk assessment system,and strengthening the construction of the professional talent team.
基金Sponsored by the Fundamental Research Funds for the Central Universities(Grant No.N110304008)the National Natural Science Foundation of China(Grant No.61374137)
文摘A modified two-degrees-of-freedom( M-TDOF) internal model control( IMC) method is proposed for non-square systems with multiple time delays and right-half-plane( RHP) zeros. In this method,pseudo-inverse is introduced to design the internal model controller,and a desired closed-loop transfer function is designed to eliminate the unrealizable factors of the derived controller. In addition,set-point tracking and load-disturbance rejection of each process are separately controlled by two controllers. The simulation results show that in addition to high decoupling performance and robustness,the proposed control method also effectively improves loaddisturbance rejection and simultaneously optimizes the input tracking performance and disturbance rejection performance by selecting the parameters of controllers. Furthermore,the higher tolerance of model mismatch is achieved in this paper.
基金The National High Technology Research&Development Program of China under contract No.2012AA10A411the National Natural Science Foundation of China under contract Nos 41176151 and 41276177
文摘Pyropia haitanensis is an economically important mariculture crop in China and has a high research value for several life phenomena, for example environmental tolerance. To explore the mechanisms underlying these characteristics, gene expression has been investigated at the whole transcriptome level. Gene expression studies using quantitative real-time PCR should start by selecting an appropriate internal control gene; therefore, the absolute expression abundance of six housekeeping genes (18S rRNA (18S), ubiquitin-conju-ating enzyme (UBC), actin (ACT), β-tubulin (TUB), elongation factors 2 (EF2), and glyceraldehyde-3-phos- phate dehydrogenase (GAPDH) examined by the quantitative real-time PCR in samples corresponding to different strains, life-cycle stages and abiotic stress treatments. Their expression stabilities were assessed by the comparative cycle threshold (Ct) method and by two different software packages: geNorm and NormFinder. The most stable housekeeping gene is UBC and the least stable housekeeping is GADPH. Thus, it is proposed that the most appropriate internal control gene for expression analyses in P. haitanensis is UBC. The results pave the way for further gene expression analyses of different aspects of P. haitanensis biology including different strains, life-history stages and abiotic stress responses.
基金the National Natural Science Foundation of China,No.81901257(to YXW)the Natural Science Foundation of Jiangsu Province of China,No.BK20180951(to YXW)+1 种基金Postgraduate Research and Practice Innovation Program of Jiangsu Province of China,No.KYCX20_2818(to WL)and Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD,to Key Laboratory of Neuroregeneration of Jiangsu and Ministry of Education).
文摘There is a lack of systematic research on the expression of internal control genes used for gene expression normalization in real-time reverse transcription polymerase chain reaction in spinal cord injury research.In this study,we used rat models of spinal cord hemisection to analyze the expression stability of 13 commonly applied reference genes:Actb,Ankrd27,CypA,Gapdh,Hprt1,Mrpl10,Pgk1,Rictor,Rn18s,Tbp,Ubc,Ubxn11,and Ywhaz.Our results show that the expression of Ankrd27,Ubc,and Tbp were stable after spinal cord injury,while Actb was the most unstable internal control gene.Ankrd27,Ubc,Tbp,and Actb were consequently used to investigate the effects of internal control genes with differing stabilities on the normalization of target gene expression.Target gene expression levels and changes over time were similar when Ankrd27,Ubc,and Tbp were used as internal controls but different when Actb was used as an internal control.We recommend that Ankrd27,Ubc,and Tbp are used as internal control genes for real-time reverse transcription polymerase chain reaction in spinal cord injury research.This study was approved by the Administration Committee of Experimental Animals,Jiangsu Province,China(approval No.20180304-008)on March 4,2018.
基金Project(61273132)supported by the National Natural Foundation of ChinaProject(20110010010)supported by Higher School Specialized Research Fund for the Doctoral Program,China
文摘A novel control scheme of active disturbance rejection internal model control(ADRIMC) is proposed to improve the anti-interference ability and robustness for the dead-time process. The active anti-interference concept is introduced into the internal model control(IMC) by analyzing the relationship between IMC and disturbance observer control(DOB). Further, a design process of disturbance filter is presented to realize the active anti-interference ability for ADRIMC scheme. The disturbance filter is used to estimate an equivalent disturbance consisting of both external disturbances and internal disturbances caused by model mismatches.Simulation results demonstrate that the proposed method possesses a good disturbance rejection performance, though losing some partial dynamic performance. In other words, the proposed method shows a tradeoff between the dynamic performance and the system robust.
基金Supported by National Natural Science Foundation of China(Grant No.51375009)PhD Research Foundation of Liaocheng University,China(Grant No.318051523)Tsinghua University Initiative Scientific Research Program,China
文摘Because of vehicle's external disturbances and model uncertainties,robust control algorithms have obtained popularity in vehicle stability control.The robust control usually gives up performance in order to guarantee the robustness of the control algorithm,therefore an improved robust internal model control(IMC) algorithm blending model tracking and internal model control is put forward for active steering system in order to reach high performance of yaw rate tracking with certain robustness.The proposed algorithm inherits the good model tracking ability of the IMC control and guarantees robustness to model uncertainties.In order to separate the design process of model tracking from the robustness design process,the improved 2 degree of freedom(DOF) robust internal model controller structure is given from the standard Youla parameterization.Simulations of double lane change maneuver and those of crosswind disturbances are conducted for evaluating the robust control algorithm,on the basis of a nonlinear vehicle simulation model with a magic tyre model.Results show that the established 2-DOF robust IMC method has better model tracking ability and a guaranteed level of robustness and robust performance,which can enhance the vehicle stability and handling,regardless of variations of the vehicle model parameters and the external crosswind interferences.Contradiction between performance and robustness of active steering control algorithm is solved and higher control performance with certain robustness to model uncertainties is obtained.
文摘An adaptive internal mode control is proposed to eliminate effectively periodic disturbance with uncertain frequency caused by input error angle of PIGA (Pendulous Integrating Gyro Accelerometer). An adaptive algorithm with periodic disturbance frequency identification on line is applied and the internal model controller parameters are adjusted to eliminate disturbance. Then the convergence of this algorithm and the stability of the system are proved by the averaging method. Simulation results verify the proposed scheme can eliminate periodic disturbance and improve the test precision for PIGA effectively.
文摘PID controllers were used for the hydraulic servo system of sliding gate and the tundish weight control system in continuous caster.These two loops were synthesized in mould level controller based on model reduction and internal model control strategy.Satisfactory control performance of this synthetic mould level controller was demonstrated by simulations and on-line experiments.