Exploration of the Clinical Rule of Acupuncturemoxibustion Treatment for Lumbar Intervertebral Disk Herniation

Objective： To review year 1956-2007 literature on acupuncture and moxibustion and explore the regularity of point selection and methods for acupuncture-moxibustion treatment of lumbar intervertebral disk herniation. Methods： A comprehensive analysis was made by a method of metrological analysis according to the data from ＂China modem acupuncture-moxibustion information databank＂. Results： Points Huantiao （GB 30）, Weizhong （BL 40）, Yanglingquan （GB 34）, and Jiaji （Ex-B 2） were most frequently used. Acupuncture was the most frequently used therapy and electroacupuncture, the next one. Conclusion： Electroacupuncture is a common and effective way to treat lumbar intervertebral disk herniation. Points Huantiao （GB 30）, Weizhong （BL 40）, Yanglingquan （GB 34）, and Jiaji （Ex-B 2） can be used as a basic prescription for acupuncture-moxibustion treatment of lumbar intervertebral disk herniation. Other acupoints are selected according to the syndrome.

Treatment of 52 Cases of Lumbar Intervertebral Disk Herniation by Acupuncture plus Herbal Medicine

Lumbar intervertebral disk herniation is a commonly and frequently encountered disease in the orthopedic department, manifested by a series of symptoms such as low back pain, numbness and pain in one or two lower limbs, due to compression to the lumbosacral nerves by protrusion of the pulpiform nucleus after rupture of the fibrous rings. The author has treated 52 cases of this disease by acupuncture plus herbal medicine. Now, the report is given as follows.

Treatment of Lumbar Intervertebral Disk Herniation with Electric Acupuncture

Objective： To make objective evaluation on treatment of lumbar intervertebral disc herniation with electric acupuncture. Method： All 200 cases were randomly allocated into an observation group （100 cases） and a control group （100 cases）. Cases in the observation group were treated with electric acupuncture. Cases in the control group were treated with oral administration of Mobic. Result： The total effective rate in the observation group was 86.5%, and the total effective rate of the control group was 75.0%. There was a significant difference between the two groups （P〈0.01）. Conclusion： Electric acupuncture is a safe and remarkably effective way for lumbar intervertebral disc herniation.

Association Between VDR FokI Polymorphism and Intervertebral Disk Degeneration

Intervertebral disk degeneration （IDD） is strongly associated with genetic predisposition and environmental susceptibility. Several studies been conducted to investigate the potential asso- ciation between IDD and FokI polymorphism located in the gene encoding the vitamin D receptor （VDR）, and inconsistent conclusions had been reached among different ethnic populations. In order to assess the association between the FokI polymorphism and the risk of IDD, we performed a comprehensive and systematic meta-analysis. Candidate articles were retrieved from PubMed, EMBASE, China National Knowledge Infrastructure （CNKI）, and China Biology Medical （CBM） with strict inclusion criteria in January 2015. Among the 54 articles that were retrieved, only eight studies met the inclusion criteria. The pooled data analysis based on allele contrast, homozy- gore, heterozygote, dominant, and recessive models revealed no significant correlation between the FokI polymorphism and the risk of IDD. However, when stratified by ethnicity, significant associ- ations were detected for Hispanics based on allele contrast （OR = 1.395, 95% CI = 1.059-1.836, P = 0.018）, homozygote （OR = 1.849, 95% CI = 1.001-3.416, P = 0.049）, heterozygote （OR = 1.254, 95% CI = 1.049-1.498, P = 0.013）, and dominant （OR = 1.742, 95% CI = 1.174-2.583, P = 0.006） models, and for Asians using the dominant model （OR = 1.293, 95% CI = 1.025-1.632, P --- 0.030）, whereas there is no significant association detected for Caucasians. In conclusion, FokI polymorpbism is not generally associated with IDD, but there is increased risk for IDD in Hispanics and Asians carrying FokI allele T.

Treatment of 120 Cases of Lumber Intervertebral Disk Displacement by Electroacupuncture plus Movable Cupping and Traction

Ashi points, movable cupping and traction were employed to treat 120 cases of prolapase of lumber intervertebral disk, and the total effective rate was 95.6%. Key Words Lumber Vertebrae - Electroacupuncture - Cupping Therapy - Acupuncture-moxibustion - Traction - Intervertebral Disk Dislacement Author: Lü Guo-ping (1958-), female, attending physicianTranslator: WU Xue-fei

Treatment of 150 Cases of Lumber Intervertebral Disk Displacement by Acupuncture plus Traction

Needling Point Jiaji (Ex-B 2), Dachangshu (BL 25), Huantiao (GB 30), Weizhong (BL 40), and Yanglingquan (GB 34) etc with the cooperation of traction, treated lumbar intervertebral disk displacement. The effective rate was 91.3%. Key Words Lumbar Vertebrae - Intervertebral Disk Displacement - Acupuncture Therapy - Traction Translator: WU Xue-fei

Treatment of Protrusion of Lumbar Intervertebral Disc by Pulling and Turning Manipulations

The authors treated 62 cases of protrusion oflumbar intervertebral disc by pulling and turningmanipulations from February 1993 to February 1998.Another 50 cases were treated by traction as controls.Statistical data showed that pulling and turningmanipulations had much better therapeuticeffectiveness than traction(P<0.05).

Treatment of Protrusion of the Lumbar Intervertebral Disc by TCM Massage

Protrusion of the lumbar intervertebral disc is a common disorder frequently encountered in clinic. With TCM massage and the maneuver of manual reduction, we have treated 82 cases of protrusion of the lumbar intervertebral disc, with satisfactory therapeutic results reported in the following.……

Fifty-six Cases of Protrusion of Lumbar Intervertebral Disc Treated by Penetration and Oral Administration of Chinese Decoction Plus Traction

Fifty-six cases of the protrusion of the lumbar intervertebral disc in the treatment group were treated by drug-penetration and oral administration of traditional Chinese decoction plus traction, and the other 35 cases in the control group by oral administration of Chinese decoction and traction. The results showed that the cure rate in the treatment group was 83.9%, and that in the control group was 57.1%, with a statistically significant difference between the two groups (P<0.01), indicating that the former is a more effective therapy for protrusion of the lumbar intervertebral disc.

Treatment of Protrusion of the Lumbar Intervertebral Disc by Intravertebral Blocking and Massotherapy——A Report of 62 Cases

Protrusion of the lumbar intervertebral disc is adisease commonly encountered in orthopedics andtraumatology clinic.At present removal of thenucleus pulposus to relieve the compression on thenerve root is usually adopted in the treatment of thedisease.From April,1990 to October,1997,theauthor treated 62 cases of protrusion of the lumbarintervertebral disc by massotherapy afterintravertebral blocking,with satisfactory

Treatment of Protrusion of the Lumbar Intervertebral Disc by Massotherapy

Clinically, there is a higher incidence of protrusion of the lumbar intervertebral disc. It can cause a terrible pain. The author has treated 66 cases by massotherapy, who were confirmatively diagnosed as having protrusion of the lumbar intervertebral disc by X-ray film and computer-aided tomography, with satisfactory results as reported in the following.Clinical DataAmong the 66 cases in this series, 49 were male and 17 female, ranging in age from 26 to 59 years, averaging 36.2 years. All the patients had got lumbago and unilateral ischialgia, with the left side affected in 37 cases and the right side in 29 cases. The leg pain radiating to lateral malleolus was found in 28 cases, to dorsum of the foot in 24 cases, and to the toes in 14 cases. Intermittent claudication was present in 22 cases, numbness of the affected foot in 16 cases, pain exacerbated when coughing in 35 cases, scoliosis in 21 cases, and positive Lasegue's sign in 48 cases.

TCM Treatment for 50 Cases of Acute Protrusion of the Lumbar Intervertebral Disc

From January 1998 to August 2000, the author has treated 50 cases of acute protrusion of the lumbar intervertebral disc by Chinese herbs, massotherapy, and functional exercise with satisfactory therapeutic results reported in the following.

Protrusion of Lumbar Intervertebral Disc Treated by Ultra Short Wave and the Point Injection in 40 Cases

Since 1996,the author has treated 40 cases of protrusion of lumbar intervertebral disc with ultra short wave and the point injection,and obtained good therapeutic effects.This is reported as follows.

Effect of siRNA interference on nerve growth factor in intervertebral disc inflammation rats

Objective:To investigate the inhibition effect of siRNA interference on NGF induced by inflammatory factor IL-6,and JUL—1 so as to provide novel targets for clinical treatment of discogenic low back pain.Methods:The intervertebral disc nucleus and annulus fibrosus cells of rats were separated-The cells were co-cultured with different concentrations(10 nmol/L,20nmol/L,50 nmol/L,100 nmol/L)of IL-6 and IL-1β.The NGF-siRNA was leaded into the cocultured cells with its import ability assessed by flow cytometry instrument tests,hefore and after which the NCF mRNA expression was detected by real-time Q-PCR and the NGF content was detected by ELISA.Results:Flow cytometry instrument test results showed that the NGFsiRNA cell conversion rate was 99.8%.Real-time Q-PCR detection results showed that compared with negative control group,the NGF mRNA expression of co-cultured cells treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L IL-6 and IL-1βwere respectively raised 3.4,3.7,4.7,3.7 times which were all significantly down-regulated after the import of NGF-siRNA.EILSA detection results showed that compared with negative control group,the NGF content of cocultured medium treated by 10 nmol/L,20 nmol/L,50 nmol/L,100 nmol/L I-L6 and IL-1βwere respectively raised 2.9,3.3,4.5,7.4 times which were all significantly decreased after the import of NGF-siRNA.Conclusions:These molecular biological results suggest that inflammatory factor IL-6 and IL-1βcould stimulate NCF on intervertebral disc cells in vitro culture model and its efficiency is concentration dependent,while siRNA interference can inhibit the stimulation effect of IL-6 and IL-1βon intervertebral disc cell,which provides a new targets for the clinical treatment of discogenic low back pain.

经皮椎间孔入路内窥镜治疗腰椎间盘突出症的临床疗效及微创性对比研究

目的对经皮椎间孔入路内窥镜与传统后路开放手术的临床疗效及微创性进行评估。方法选择2008年10月到2009年4月于我院接受手术的腰椎间盘突出症患者,根据病情及自身选择等因素分为试验组和对照组,试验组采取经皮椎间孔镜下椎间孔入路腰椎间盘切除术,共33例;对照组采取传统后路开放手术,共37例。对两种术式系列指标包括术中出血量、手术持续时间、切口长度、术后下床时间、术后住院天数、并发症,及视觉模拟评分、Macnab评分以及白细胞数、中性粒细胞数、血红细胞沉降率、血浆C反应蛋白、卧位血浆醛固酮、血浆血管紧张素Ⅱ、血浆肾素活性进行测量比较分析,并行统计学处理。结果 1试验组较对照组切口小,术中出血量少,住院天数少,手术时间短,术后下床时间早,两组结果差异有统计学意义(P<0.05)。2两组术后1月、末次随访VAS评分和术前相比明显下降,两组术后1月、末次随访VAS评分较术前相比明显升高;两组术前、术后1月、末次随访VAS评分差异无统计学意义(P>0.05)。3试验组Macnab评分,术后1月优良率90.9%,截止末次随访优良率94.0%;对照组,术后1月优良率91.9%,截止末次随访,优良率94.6%。4术后1d、术后5d对照组较试验组血液沉降率、C反应蛋白、卧位血浆醛固酮、血浆血管紧张素Ⅱ、血浆肾素活性较对照组明显增加,差异无统计学意义(P>0.05),而白细胞数、中性粒细胞数差异无统计学意义(P>0.05)。结论对经皮椎间孔入路内窥镜治疗腰椎间盘突出症与传统后路开放手术相比切口小,术中出血量少,住院天数少,手术时间短,术后下床时间早、对机体的干扰相对小,应激反应更小,疗效相当。

Clinical Application of the Triple Puncture

IntroductionTriple puncture, first appeared in Miraculous Pivot (灵枢), is an ancient method of acupuncture, which has been used to treat relatively small and deep disorders caused by pathogenic wind by puncturing the center of the affected area with one needle and the surrounding areas with two other needles.

Retrospective study of acupuncture treatment for canine thoracolumbar intervertebral disc herniation

Thoracolumbar interverbal disk herniation(TL-IVDH)is a common neurological disorder in dogs,which often affects the life quality of patients and their caregivers.Acupuncture is increasingly accepted by veterinarians and animal owners,and has been used in the treatment of neurologic disorders.This study aims to evaluate the effects of acupuncture treatment for canine TL-IVDH.Clinical cases from 2016 to 2021 were collected.According to the inclusion and exclusion criteria,94 TL-IVDH cases that received acupuncture treatment were selected.Clinical classification and efficacy criteria were formulated based on the Frankel Score.For each case,clinical presentation,improvement,recovery and recurrence were recorded.Male dogs with TL-IVDH accounted for 64%of all cases.The average age was 7.49 years old,the predominant breed was poodle,and the incidence site was concentrated from T11 to L1.The overall recovery rate after receiving acupuncture treatment was 79.78%,which was comparable when considering only cases with grade 2,3,or 4 spinal cord lesions(83.33%~85.19%).In conclusion,canine with grade 2,3,and 4 spinal cord lesions due to TL-IVDH receiving acupuncture had a high recovery rate,suggesting acupuncture could be a viable treatment options for canine with TL-IVDH.

Construction of recombinant baculovirus Ac-CMV-hSox9 for gene therapy of intervertebral disc degeneration

Objective： To construct the recombinant baculovirus Ac-cytomegalovirus （CMV）-hSox9 for gene therapy of intervertebral disc degeneration. Methods： Bac-to-Bac system was used for the construction of baculovirus Ac-CMV-hSox9. The cDNA of hSox9 was first cloned into a plasmid vector under the control of CMV promotor to generate the donor plasmid pFastBacDul-green fluorescene protein （ GFP ）-CMV （pgGC）-hSox9. The resultant plasmid was transformed into DH10Bac cells and then the transformation mixture was spread on Luria-Bertani （LB） agarose culture medium containing isopropyl-β-D-thiogalactoside （IPTG）, X-gal, gentamicin, kanamycin and tetracycline. The white colonies were selected and cultured for amplification, and the hSox9 Bacmid DNA was extracted. After verification, recombinant baculovirns Ac-CMV-hSox9 was obtained through transfecting Sf 21 cells. The expression of hSox9 gene in the intervertebral disc cells in rabbits was determined by Western blotting and immunohistochemical staining. Results： Polymerase chain reaction （ PCR ） confirmed the presence of hSox9 gene in the recombinant baculovirus and the Sf 21 cells transfected by the baculovirus showed the expression of fluorescence protein. Western blotting and indicated that exogenous hSox9 disc cells. staining analysis Conclusions： The successful construction of the recombinant baculovirus Ac-CMV-hSox9 and the confirmation of the target gene expression provides a novel expression vector system for basic research and clinical treatment of intervertebral degenerative disc disease.

The influence of the intervertebral disc on stress distribution of the thoracolumbar vertebrae under destructive load

Objective: To compare the influence of normal and degenerative discs on stress distribution of the thoracolumbar vertebrae under destructive load, explore the biomechanical background and clinical meaning and provide theoretical basis for clinical diagnosis and treatment. Methods: A mechanical model of thoracolumbar motion segment of normal and degenerative discs was built with a three dimensional finite element method and three stresses of vertical compression, compressive flexion and distractive flexion were comparatively analyzed. Results: With vertical compression and compressive flexion loads, the thoracolumbar motion segment of the normal disc showed that the central part of the upper and lower end plates of the vertebrae and the central part of the trabecular bone adjacent to the end plate were loaded with the most intensive stresses, meanwhile, the postero lateral part of the annulus fibrosus was concentrated with stresses. Degenerative disc showed that the stress distribution of the trabecular bone was relatively averaged, the stresses of the central part adjacent to the end plate were low, while at the same time, the stresses of the peripheral part were elevated relatively. With distraction flexion load, the stresses of the cortex bone, trabecular bone, end plate and annulus fibrosus of the thoracolumbar vertebrae of degenerative discs were low, meanwhile, the stresses of the posterior structure of the vertebral body were relatively elevated compared with that of normal discs. Conclusions: There is difference in influence between normal and degenerative discs on the stress distribution of the thoracolumbar vertebrae with destructive load. The transferring way of load is changed after disc degeneration.

Electroacupuncture and Waist-building Exercise in Treating Lumbar Disk Herniation

Objective： To observe the clinical efficacy of electroacupuncture and waist-building exercise in treating lumbar disk herniation. Methods： A multi-center, randomized controlled trial was adopted. Three hundred cases of lumbar disk herniations were divided into two groups, an observation group in which 149 cases were treated by electroacupuncture and waist-building exercise, and a control group in which 151 cases were treated by electroacupuncture alone. The clinical efficacy and long-term relapse rate were observed and compared between the two groups. Results： Among 149 cases in the observation group, 80 cases were cured, 57 cases improved, 12 cases failed and 4 cases relapsed; the cure rate was 53.7%, the total effective rate was 91.9% and the relapse rate was 5.0%. Among 151 cases in the control group, 74 cases were cured, 51 cases improved, 26 cases failed and 12 cases relapsed; the cure rate was 49.0%, the total effective rate was 82.8% and the relapse rate was 16.2%. There were significant differences in the cure rate and the total effective rate between the two groups （P〈0.05）. Conclusion： Combined electroacupuncture and waist-building exercise had better effects than electroacupuncture in treating lumbar disk herniation.