THE ASSOCIATION OF Ala54Thr VARIANT OF INTESTINAL FATTY ACID BINDING PROTEIN GENE WITH GENERAL AND REGIONAL ADIPOSE TISSUE DEPOTS

Objective. To ascertain the relationship between the Ala54Thr variation of FABP2 gene and general as well as regional adipose tissue depots. Subjects. 165 subjects, in which 86 were subjects with normal glucose tolerance (NGT) [age 54 45±9 80, male/female 1 05,body mass index (BMI)26 48±4 01] and 79 were subjects with non insulin dependent diabetes mellitus (NIDDM)(age 55 86±10 00,male/female 1 08,BMI 26 75±3 30). Design and measurements. An association study of FABP2 Ala54Thr variation detected by PCR/HhaI digestion with general and regional adipose tissue depots determined by BMI and magnetic resonance imaging [abdominal subcutaneous and visceral adipose tissue area (SA and VA) and femoral subcutaneous adipose tissue area (FA)]. Results. The geneotype and allele frequencies of FABP2 Ala54Thr variation in Chinese were quite close to the frequencies in American Caucasians and Pima Indians reported in the literature. Significant difference in genotype frequency distribution was observed between FA subgroups comparisons (FA≥75cm 2 versus FA<75cm 2 )in NIDDM subjects (X 2 =11 460,P=0 003),with significantly increased in Thr54 carrier[Thr54(+)]genotype frequency and Thr54 allele frequency in NIDDM subject with FA<75cm 2 (odd ratio for genotype was 4 62,X 2 =10 112,P=0 001;and for allele=2 36,X 2 =5 379,P=0 020).The FA in NIDDM Thr54(+)subgroup was significantly lower than that in subjects with NIDDM Thr54( )sugroup(61 19±21 51cm 2 versus 75 36±31 70cm 2 ,P=0 021). Stepwise regression analysis revealed that FABP2 Thr54 genotype variation was an independent factor contributing to the variation of FA in NIDDM(P=0 003). Conclusion. FABP2 is associated with regional adipose tissue depot.The decreased femoral subcutaneous adipose tissue depot in NIDDM subjects is related to FABP2 Thr54 variant.

Fatty acid binding protein 5 is a novel therapeutic target for hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC)is an aggressive subtype of liver cancer and is one of the most common cancers with high mortality worldwide.Reprogrammed lipid metabolism plays crucial roles in HCC cancer cell survival,growth,and evolution.Emerging evidence suggests the importance of fatty acid binding proteins(FABPs)in contribution to cancer progression and metastasis;however,how these FABPs are dysregulated in cancer cells,especially in HCC,and the roles of FABPs in cancer progression have not been well defined.AIM To understand the genetic alterations and expression of FABPs and their associated cancer hallmarks and oncogenes in contributing to cancer malignancies.METHODS We used The Cancer Genome Atlas datasets of pan cancer and liver hepatocellular carcinoma(LIHC)as well as patient cohorts with other cancer types in this study.We investigated genetic alterations of FABPs in various cancer types.mRNA expression was used to determine if FABPs are abnormally expressed in tumor tissues compared to non-tumor controls and to investigate whether their expression correlates with patient clinical outcome,enriched cancer hallmarks and oncogenes previously reported for patients with HCC.We determined the protein levels of FABP5 and its correlated genes in two HCC cell lines and assessed the potential of FABP5 inhibition in treating HCC cells.RESULTS We discovered that a gene cluster including five FABP family members(FABP4,FABP5,FABP8,FABP9 and FABP12)is frequently co-amplified in cancer.Amplification,in fact,is the most common genetic alteration for FABPs,leading to overexpression of FABPs.FABP5 showed the greatest differential mRNA expression comparing tumor with non-tumor tissues.High FABP5 expression correlates well with worse patient outcomes(P<0.05).FABP5 expression highly correlates with enrichment of G2M checkpoint(r=0.33,P=1.1e-10),TP53 signaling pathway(r=0.22,P=1.7e-5)and many genes in the gene sets such as CDK1(r=0.56,P=0),CDK4(r=0.49,P=0),and TP53(r=0.22,P=1.6e-5).Furthermore,FABP5 also correlates well with two co-expressed oncogenes PLK1 and BIRC5 in pan cancer especially in LIHC patients(r=0.58,P=0;r=0.58,P=0;respectively).FABP5high Huh7 cells also expressed higher protein levels of p53,BIRC5,CDK1,CDK2,and CDK4 than FABP5low HepG2 cells.FABP5 inhibition more potently inhibited the tumor cell growth in Huh7 cells than in HepG2 cells.CONCLUSION We discovered that FABP5 gene is frequently amplified in cancer,especially in HCC,leading to its significant elevated expression in HCC.Its high expression correlates well with worse patient outcome,enriched cancer hallmarks and oncogenes in HCC.FABP5 inhibition impaired the cell viability of FABP5high Huh7 cells.All these support that FABP5 is a novel therapeutic target for treating FABP5high HCC.

Sequencing of Intron 3 of Porcine Heart Fatty Acid-Binding Protein Gene

[ Objective] The aim of this paper is to provide the basic data for marker-assisted selection of pig breeding using porcine heart fatty acid- binding protein （H-FABP） gene. [Method] According to the related sequences of porcine H-FABP gene released in GenBank, specific primers were designed to amplify the intron 3 of porcine H-FABP gene. [ Result] The intron 3 of porcine H-FABP gene was amplified successfully. Its whole sequence was 1 350 bp in length and had been submitted to GenBank （Accession no. ： DQ 002993）. [Condusion] The study lays a theoretical foundation for determination of the major genes affecting intramuscular fat deposition.

Platycodon grandiflorum extract represses up-regulated adipocyte fatty acid binding protein triggered by a high fat feeding in obese rats

AIM: To investigate the effect of Platycodon grandi- florum extract (PGE) on lipid metabolism and FABP mRNA expression in subcutaneous adipose tissue of high fat diet-induced obese rats. METHODS: PGE was treated to investigate the inhibitory effect on the pre-adipocyte 3T3-L1 differentiation and pancreatic lipase activity. Male Sprague-Dawley rats with an average weight of 439.03 ± 7.61 g were divided into four groups: the control groups that fed an experimental diet alone (C and H group) and PGE treatment groups that administered PGE along with a control diet or HFD at a concentration of 150 mg/kg body weight (C + PGE and H + PGE group, respectively) for 7 wk. Plasma total cholesterol (TC) and triglycerol (TG) concentrations were measured from the tail vein of rats. Adipocyte cell area was measured from subcutaneous adipose tissue and the fatty acid binding protein (FABP) mRNA expression was analyzed by northern blot analysis. RESULTS: PGE treatment inhibited 3T3-L1 pre-adipocyte differentiation and fat accumulation, and also decreased pancreatic lipase activity. In this experiment, PGE signifi cantly reduced plasma TC and TG concentrations as well as body weight and subcutaneous adipose tissue weight. PGE also significantly decreased the size of subcutaneous adipocytes. Furthermore, it significantly repressed the up-regulation of FABP mRNA expression induced by a high-fat feeding in subcutaneous adipose tissue. CONCLUSION: PGE has a plasma lipid lowering-effect and anti-obesity effect in obese rats fed a high fat diet.From these results, we can suggest the possibility that PGE can be used as a food ingredient or drug component to therapeutically control obesity.

Role of Heart-Type Fatty Acid Binding Protein in Early Detection of Acute Myocardial Infarction in Comparison with cTnI, CK-MB and Myoglobin

Heart fatty acid-binding protein (H-FABP) is supposed to be the most sensitive biomarker of early acute myocardial infarction (AMI). To evaluate the diagnostic value of H-FABP for AMI in the early stage, the plasma levels of H-FABP were measured by sandwich ELISA in 93 patients with suspected AMI at admission within 6 h after onset of chest pain and 69 normal healthy subjects. The plasma concentrations of cardiac troponin-I (cTnI), creatine kinase-MB (CK-MB) and myoglobin (Mb) were assayed at the same time by using corpuscle chemiluminescence for those patients. The patients were classified as AMI group (n=32) and non-AMI group (n=61) retrospectively. The diagnostic validity was evaluated in terms of sensitivity, specificity and receiver operating characteristic (ROC) curve analysis. The results showed the cutoff value of H-FABP for AMI was 16.8 ng/ml, and its diagnostic sensitivity for AMI was 64.29 % within 3 h and 84.38 % within 6 h after onset of chest pain, and the diagnostic specificity for non-AMI was 100 % within 3 h and 91.8 % within 6 h. H-FABP had higher sensitivity than that of cTnI and CK-MB at all time points (P<0.05), whereas there was no significant difference in specificity among the four markers. But the area under the ROC curve of H-FABP was significantly greater than that of cTnI, CK-MB and Mb within 3 h. These results revealed that H-FABP possessed high diagnostic sensitivity and specificity for AMI in early stage, especially within 3 h after onset of persistent angina pectoris. In conclusion, H-FABP can be used as a sensitive marker for AMI in the early stage.

Supplementing the early diet of broilers with soy protein concentrate can improve intestinal development and enhance short-chain fatty acid-producing microbes and short-chain fatty acids,especially butyric acid

Background:Research on nutrition in early-life commonly focuses on the maturation of the intestine because the intestinal system is crucial for ensuring continued growth.To explore the importance of early nutrition regulation in animals,soy protein concentrate(SPC)was added to the early diet of broilers to investigate its effects on amino acid digestibility,intestinal development,especially intestinal microorganisms,and broiler metabolites.A total of 192 oneday-old Arbor Acres(AA)male broilers were randomly assigned to two experimental treatments with 8 replicates of 12 birds.The control group was fed a basal diet(control),and the treatment group was fed a basal diet supplemented with 12%SPC(SPC12)during the first 10 d(starter phase).From d 11 to 21(grower phase)and d 22 to 42(finisher phase),a basal diet was fed to both treatment groups.Results:SPC reduced the pH value and acid-binding capacity of the starter diet(P<0.05,d 10);SPC in the early diet enhanced the gizzard weight(P<0.05,d 10 and d 42)and the ileum weight(P<0.05,d 10)and decreased the weight and length of the jejunum(P<0.05,d 10)and the relative length of the duodenum and jejunum(P<0.05,d 10).At the same time,SPC enhanced villus height(P<0.05,d 10)and muscle thickness in the jejunum and ileum(P<0.05,d 10)and increased the number of goblet cells in the duodenum(P<0.05,d 10).Meanwhile,SPC increased the Chao1 index and the ACE index(P<0.05,d 10)and altered the composition of caecal microflora at d 10.SPC also increased the relative abundance of Alistipes,Anaerotruncus,Erysipelatoclostridium,Intestinimonas and Flavonifractor bacteria(P<0.05,d 10).At the same time,the concentrations of caecal butyric acid and total short-chain fatty acids(SCFAs)were also increased in the SPC12 group(P<0.05,d 10).Conclusions:In summary,the results showed that supplementing the starter diet of broilers with SPC has a significant effect on the early development of the intestine and the microflora.

Fatty acid binding receptors in intestinal physiology and pathophysiology

Free fatty acids are essential dietary components and recognized as important molecules in the maintenance of cellular homeostasis.In the last decade,the molecular pathways for free fatty acid sensing in the gastrointestinal tract have been further elucidated by molecular identification and functional characterization of fatty acid binding receptors.These sensing molecules belong to the family of G proteincoupled receptors.In the intestine,four important receptors have been described so far.They differ in molecular structure,ligand specificity,expression pattern,and functional properties.In this review,an overview of intestinal fatty acid binding receptors and their role in intestinal physiology and pathophysiology is given.

Expression and Characterization of Human Heart Type Fatty Acid Binding Protein in Pichia Pastoris

H-FABP is regarded as a tissue-specific protein existing only in myocardial cells. It is released from the cardiac tissue and gets into the plasma when a heart attack occurs; the myocardial infarction is a good case in point. As a resuit, the detection of H-FABP will be an early and important biomarker for the disease concerned. The objective of the study is to prepare the recombinant H-FABP by aeukaryotic expression system, pichia, to produce the protein mimicking natural H-FABP, as an immunogen for the production of the specific antibody. A gene fragment encoding H-FABP was cloned in the expressing vector pPICZα, after sequencing. The recombinant plasmid was transformed into the competent cells of the X-33 strain by means of electroporation. The expression of the target peptide induced by methanol was screened by means of Western hlotting, with the available MAb（Clone 6B6）. Highly expressive engineer strains were obtained. The production of recombinant H-FABP under induction was about 0.7 g/L, with an Mr of 14.5 kDa and recognized by a commercially available MAb （Clone 6B6）. The recombinant vector was successfully constructed. Following this, H-FABP was expressed in X-33, and it would become the source of the preparation of specific antibodies, to develop diagnostic kits.

Correlation study of serum epithelial fatty acid binding protein with glucose and lipid metabolism and micro inflammatory reaction in obese children

Objective: To study the correlation of serum epithelial fatty acid binding protein (E-FABP) with glucose and lipid metabolism and micro inflammatory reaction in obese children. Methods: children diagnosed as simple obesity in endocrinology department of my hospital during June 2014 – August 2017 were selected as the obese group, and the health examination children were selected as the control group. The serum was collected and the levels of E-FABP, glucose and lipid metabolism and inflammatory cytokines were determined, peripheral blood was collected and the expression level of insulin signal molecules were measured. Results:serum E-FABP content of obese group was significantly higher than that in the control group;serum total cholesterol (TC), triglyceride (TG), low density lipoprotein cholesterol (LDL-C), Leptin, Chemerin, F-INS, tumor necrosis factor - α (TNF-α), interleukin -1β (IL-β), interleukin-6 (IL-6), soluble intercellular adhesion molecule -1 (sICAM-1) and monocyte chemoattractant protein 1 (MCP1) of obese group were significantly higher than thosein the control group and positively correlated with serum E-FABP content;serum high density lipoprotein cholesterol (HDL-C), lipoprotein (APN), and C1q/tumor necrosis factor-related proteins 12 (CTRP12), Omentin-1 and the expression intensity of insulin receptor substrate 1 (IRS1), IRS2, glucose transporter -4 (GLUT-4) in peripheral blood were significantly lower than those of the control group and negatively correlated with serum E-FABP content. Conclusion: the excessive secretion of E-FABP in obese children is closely related to the disorder of glucose and lipid metabolism and the activation of micro inflammatory reaction.

The impact of codon 54 variation in intestinal fatty acid binding protein gene on the pathogenesis of diabetes mellitus in Chinese

Objective To investigate whether or not the intestinal fatty acid binding protein gene (FABP2) Ala54Thr variation is related to non insulin dependent diabetes mellitus (NIDDM), obesity, dyslipidemia and glucose stimulated insulin secretion (GSIS) in Chinese.Methods The FABP2 Ala54Thr variation was detected by PCR/HhaI digestion in 231 Chinese subjects (116 with normal glucose tolerance (NGT), 54 with impaired glucose tolerance (IGT) and 61 with NIDDM). Plasma glucose, insulin and C peptide levels before and after 75 g glucose load as well as fasting lipid profile were determined.Results (1) The Ala54 and Thr54 allele frequencies in Chinese were 0.71 and 0.29 respectively; (2) The FABP2 Ala54Thr variation was neither associated with fasting and post challenged plasma glucose levels nor with NIDDM; (3) This variation was neither associated with fasting lipid profile nor with obesity; (4) The IGT subjects with genotype Thr54(+) (Thr54 homozygotes and heterozygotes) had lower fasting, 2 hour and total C peptide levels and smaller AUC representing lesser C peptide secretion after glucose challenge than those with genotype Thr54( ) (Ala54 homozygotes) (P= 0.04 , 0.03, 0.01 and 0.01 respectively). The serum insulin levels changed in the same tendency.Conclusions The glucose stimulated insulin secretion (GSIS) reserve of islet beta cells is more limited in subjects with FABP2 Thr54(+) genotype than in those with FABP2 Thr54(-) genotype. It suggests that FABP2 codon 54 variation might contribute to the insufficient insulin secretion in the development of NIDDM in Chinese.

Activation of pregnane X receptor sensitizes alcoholic steatohepatitis by transactivating fatty acid binding protein 4

Alcoholic steatohepatitis (ASH) is a liver disease characterized by steatosis, inflammation, and necrosis of the liver tissue as a result of excessive alcohol consumption. Pregnane X receptor (PXR) is a xenobiotic nuclear receptor best known for its function in the transcriptional regulation of drug metabolism and disposition. Clinical reports suggested that the antibiotic rifampicin, a potent human PXR activator, is a contraindication in alcoholics, but the mechanism was unclear. In this study, we showed that the hepatic expression of fatty acid binding protein 4 (FABP4) was uniquely elevated in ASH patients and a mouse model of ASH. Pharmacological inhibiting FABP4 attenuated ASH in mice. Furthermore, treatment of mice with the mouse PXR agonist pregnenolon-16α-carbonitrile (PCN) induced the hepatic and circulating levels of FABP4 and exacerbated ASH in a PXR-dependent manner. Our mechanism study established FABP4 as a transcriptional target of PXR. Treatment with andrographolide, a natural compound and dual inhibitor of PXR and FABP4, alleviated mice from ASH. In summary, our results showed that the PXR-FABP4 gene regulatory axis plays an important role in the progression of ASH, which may have accounted for the contraindication of rifampicin in patients of alcoholic liver disease. Pharmacological inhibition of PXR and/or FABP4 may have its promise in the clinical management of ASH.

LINC01667通过上调FABP5重塑肝细胞癌脂肪酸代谢

目的探讨LINC01667对脂肪酸结合蛋白5(FABP5)的调控作用及对肝细胞癌(HCC)细胞脂肪酸代谢的影响。方法采用双荧光素酶报告实验检测LINC01667与FABP5启动子的结合能力,以及对启动子活性的调控作用,采用尼罗红染色和游离脂肪酸摄取实验检测过表达LINC01667对HCC细胞内脂滴水平和细胞游离脂肪酸摄取能力的影响。应用Transwell实验检测LINC01667/FABP5调控轴在HCC细胞迁移和侵袭中的作用效应。结果LINC01667可与FABP5启动子结合,并上调FABP5表达(P<0.05);过表达LINC01667可增强HepG2及HuH7细胞中脂滴的蓄积,并且增强其对游离脂肪酸的摄取能力(P<0.05);靶向抑制FABP5后,由LINC01667介导的HCC细胞游离脂肪酸摄取能力增强效应及其迁移、侵袭能力均被显著减弱(P<0.01)。结论LINC01667可通过上调FABP5重塑HCC细胞脂肪酸代谢,进而促进HCC恶性进程。

血清游离脂肪酸和心型脂肪酸结合蛋白表达与2型糖尿病病人动脉粥样硬化斑块的关系研究

目的探讨血清游离脂肪酸(FFA)和心型脂肪酸结合蛋白(H-FABP)表达与2型糖尿病(T2DM)病人动脉粥样硬化斑块(CAP)的关系研究。方法选取2020年6月至2022年6月保定市第四中心医院接收的T2DM病人302例为研究对象。通过颈动脉超声检查进一步将研究对象分为CAP组(165例)和无CAP组(137例)。采用酶联免疫吸附测定(ELISA)检测血清FFA、H-FABP水平;采用logistic多因素回归分析CAP发生的影响因素;采用Spearman法分析血清FFA、H-FABP水平及与临床资料的相关性分析;采用受试者操作特征曲线(ROC曲线)评价血清FFA、H-FABP及其联合对CAP发生的诊断效能。结果相较于无CAP组,CAP组年龄、高血压史、C反应蛋白(CRP)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL)、载脂蛋白A(ApoA)、FFA、H-FABP[(4.63±1.43)μg/L比(3.68±1.05)μg/L]水平显著上升(P<0.05),LPa水平显著下降(P<0.05)。logistic分析显示,年龄、CRP、TG、LDL、FFA、H-FABP均是T2DM病人CAP发生的独立危险因素(P<0.05)。T2DM合并CAP病人血清FFA与H-FABP、年龄、CRP、TG、LDL水平呈正相关(rs=0.61、0.59、0.52、0.57、0.55,P<0.05)。血清H-FABP与年龄、CRP、TG、LDL水平呈正相关(rs=0.58、0.53、0.57、0.54,P<0.05)。ROC曲线结果显示血清中FFA、H-FABP水平及二者联合预测T2DM病人CAP发生的AUC分别为0.71、0.74、0.84,其中联合预测AUC显著高于二者单独预测AUC(Z=3.65、2.68,P<0.05)。结论FFA、H-FABP水平在T2DM合并CAP病人血清中升高,与年龄、CRP、TG、LDL关系密切,均有望成为T2DM病人CAP发生的诊断因子。

I-FABP、CRP联合肠系膜上动脉超声检测对新生儿坏死性小肠结肠炎的诊断价值

目的 探讨肠型脂肪酸结合蛋白(I-FABP)、C反应蛋白(CRP)联合肠系膜上动脉(SMA)超声检测对新生儿坏死性小肠结肠炎(NEC)的诊断价值。方法 选择2020年5月至2022年5月80例疑似NEC(Bell分期I期)患儿,以及同期72例确诊NEC(Bell分期II期)和40例无胃肠疾病的新生儿(对照组),行彩色多普勒超声检测SMA收缩期峰值流速(PSV)、舒张末期流速(EDV)及阻力指数(RI),同时检测血清I-FABP、CRP水平。结果 三组首检SMA超声PSV、EDV、RI比较差异显著(P<0.05),与对照组比较,疑似组PSV明显降低(P<0.05),RI明显增高(P<0.05),而EDV比较无明显差异(P>0.05);与确诊组比较,疑似组RI明显减小(P<0.05),而PSV、EDV比较无明显差异(P>0.05)。三组血清I-FABP、CRP水平具有统计学意义(P<0.05),与对照组比较,疑似组血清I-FABP、CRP水平明显提高(P<0.05);与确诊组比较,疑似组I-FABP水平明显降低(P<0.05),而CRP比较无明显差异(P>0.05)。ROC曲线分析显示,SMA超声+I-FABP+CRP联合诊断早期NEC的敏感性、特异性分别为87.1%、85.9%,AUC为0.834(95%CI:0.712~0.926),均高于单一诊断。结论 I-FABP、CRP及SMA超声检查对NEC具有诊断价值,三者联合对NEC具有较高的敏感性和特异性。

血清FABP4、GRP78及FOXO1与急性脑梗死患者预后的相关性

目的探讨脂肪酸结合蛋白4(FABP4)、葡萄糖调节蛋白78(GRP78)、叉头框转录因子O亚族1(FOXO1)与急性脑梗死(ACI)患者预后的相关性。方法选取2021年6月至2023年1月该院收治的148例ACI患者(ACI组)作为研究对象,另选取148例同期于该院进行体检的健康体检者作为对照组。根据ACI患者出院后3个月的预后情况将其分为预后良好组和预后不良组;比较各组血清FABP4、GRP78、FOXO1水平;采用多因素Logistic回归分析ACI患者预后不良的影响因素。绘制受试者工作特征(ROC)曲线分析血清FABP4、GRP78、FOXO1单独及联合检测对ACI患者预后不良的预测价值。结果ACI组血清FABP4、GRP78水平均高于对照组,FOXO1水平低于对照组,差异均有统计学意义(P<0.05)。预后良好组纳入92例,预后不良组纳入56例。预后不良组年龄、梗死灶面积均大于预后良好组,FABP4、GRP78水平均高于预后良好组,FOXO1水平低于预后良好组,差异均有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,FABP4、GRP78、FOXO1、年龄、梗死灶面积是ACI患者预后不良的影响因素(P<0.05)。ROC曲线分析结果显示,血清FABP4、GRP78、FOXO1单独及联合预测ACI患者预后不良的曲线下面积(AUC)分别为0.795、0.819、0.784、0.927;血清FABP4、GRP78、FOXO13项联合预测ACI患者预后不良的AUC优于各自单独预测的AUC(Z联合检测-FABP4=3.909,Z联合检测-GRP78=3.171,Z联合检测-FOXO1=4.494,P<0.05)。结论ACI患者血清FABP4、GRP78水平均升高,FOXO1水平降低,3项联合检测对ACI患者预后有较高的预测价值。

复方丹参滴丸联合西药治疗老年冠心病合并慢性心力衰竭临床研究

目的:观察复方丹参滴丸联合西药治疗老年冠心病合并慢性心力衰竭的临床疗效。方法:采用随机数字表法将2020年3月—2023年5月杭州市西湖区蒋村街道社区卫生服务中心收治的116例老年冠心病合并慢性心力衰竭患者分为试验组和对照组各58例。对照组给予西药治疗,试验组在对照组基础上联合复方丹参滴丸治疗。2组均连续治疗6个疗程。比较2组治疗前后美国纽约心脏病协会(NYHA)心功能分级、心功能指标[左室舒张末期内径(LVEDD)、左室收缩末期内径(LVESD)、左室射血分数(LVEF)]及血清脂蛋白相关磷脂酶A2(LP-PLA2)、心型脂肪酸结合蛋白(hFABP)、可溶性生长刺激表达基因2蛋白(sST2)的水平,并评估2组临床疗效及不良反应发生情况。结果:治疗后,试验组总有效率94.83%(55/58),高于对照组79.31%(46/58)(P<0.05)。治疗后,2组NYHA心功能分级较治疗前减轻(P<0.05),且试验组NYHA心功能分级轻于对照组(P<0.05)。治疗后,2组中医证候积分及LVEDD、LVESD、hFABP、LP-PLA2、sST2水平均较治疗前降低(P<0.05),LVEF水平升高(P<0.05),且试验组中医证候积分及LVEDD、LVESD、hFABP、LP-PLA2、sST2水平低于对照组(P<0.05),LVEF水平高于对照组(P<0.05)。试验组不良反应发生率1.72%(1/58),低于对照组12.07%(7/58)(P<0.05)。结论:复方丹参滴丸联合西药治疗老年冠心病合并慢性心力衰竭疗效确切,能有效增强患者心功能,降低心肌炎性损伤,安全性较高。

脑型脂肪酸结合蛋白、转铁蛋白对儿童脓毒症相关性脑病的预测价值

目的探讨脑型脂肪酸结合蛋白(B-FABP)、转铁蛋白预测儿童脓毒症相关性脑病(SAE)的临床价值。方法选取2021年9月至2023年8月徐州市儿童医院收治的60例脓毒症患儿,男性41例,女性19例,年龄中位数为11.60月,年龄范围为1~72个月,根据患儿合并SAE情况将患儿分为SAE组(合并意识障碍的患儿,n=30)与非SAE组(未合并意识障碍的患儿,n=30)。收集入院时24、48、72 h的格拉斯哥昏迷量表评分(GCS)、小儿危重病例评分(PCIS)及血清样本。检测血清B-FABP、转铁蛋白的表达。比较两组患儿入院时资料及GCS、PCIS、B-FABP、转铁蛋白的水平。受试者操作特征(ROC)曲线评估B-FABP、转铁蛋白预测SAE的灵敏度、特异度及截断值。分析B-FABP、转铁蛋白与GCS、PCIS的相关性。结果SAE组不同时间点B-FABP表达均高于非SAE组,转铁蛋白表达均低于非SAE组,差异有统计学意义(P<0.05);入院24 h时,B-FABP预测SAE的AUC值为0.884,灵敏度93.33%,特异度76.67%,入院48 h时,转铁蛋白预测SAE的AUC值为0.877,灵敏度93.33%,特异度76.67%。SAE组B-FABP与GCS、PCIS呈负相关,转铁蛋白与GCS、PCIS呈正相关(P<0.05)。结论血清B-FABP和转铁蛋白对儿童SAE的诊断及预后判断有一定临床价值。

Cloning and characterization of a stearoyl-ACP desaturase gene from Jatropha curcas

Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase （SAD） is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame （ORF）. Analysis in the BLAST on NCBI shows that Jatropha curcas SAD （JSAD） gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD （RSAD） is up to 89% and 96.2% respectively. Molecular modeling of JSAD indicates that its three-dimensional structure strongly resembled the crystal structure of RSAD.

Stress-induced intestinal necrosis resulting from severe trauma of an earthquake

AIM:To investigate the possible reasons and suggest therapeutic plan of stress-induced intestinal necrosis resulting from the severe trauma.METHODS:Three patients in our study were trapped inside collapsed structures for 22,21 and 37 h,respectively,The patients underwent 3-4 operations after sustaining their injuries,Mechanical ventilation,intermittent hemodialysis and other treatments were also provided.The patients showed signs of peritoneal irritation on postoperative days 10-38.Small intestinal necrosis was confirmed by emergency laparotomy,and for each patient,part of the small bowel was removed.RESULTS:Two patients who all performed 3 operations died of respiratory complications on the first and second postoperative days respectively.The third patient who performed 4 operations was discharged and made a full recovery.Three patients had the following common characteristics:(1) Multiple severe trauma events with no direct penetrating gastrointestinal injury;(2) Multiple surgeries with impaired renal function and intermittent hemodialysis treatment;(3) Progressive abdominal pain and tenderness,and peritoneal irritation was present on post-traumatic days 10-38;(4) Abdominal operations confirmed segment ulcer,necrosis of the small intestine,hyperplasia and stiffness of the intestinal wall;and(5) Pathological examinations suggested submucosal hemorrhage,necrosis,fibrosis and hyalinization of the vascular wall.Pathological examinations of all 3 patients suggested intestinal necrosis with fistulas.CONCLUSION:Intestinal necrosis is strongly associated with stress from trauma and post-traumatic complications;timely exploratory laparotomy maybe an effective method for preventing and treating stressinduced intestinal necrosis.

Specific protein-urea interactions

The mechanism of urea's action in protein denaturation remains largely unknown.To provide an experimental basis for molecular dynamics(MD)simulations on urea-protein interactions,we investigated the effect of urea on human intestinal fatty acid binding protein(hIFABP)by nuclear magnetic resonance(NMR).Hydrogen-deuterium exchange(HDX)rates at
2 M urea indicate that urea affects hIFABP in a residue-specific manner via direct urea-protein interactions and preferentially weakens hydrogen bonds between highly protected amides.Residue-specific effects of urea on NMR peak intensities and chemical shifts further support the presence of direct urea-protein interactions.Twodimensional(2D)water-rotating frame Overhauser enhancement(ROE)data shows one protein-bound water molecule in contact with Val66 and Trp82,one putative bound water molecule in interaction with Thr76 and E-F loop,and that urea at low concentrations cannot displace these protein-bound water molecules.Our urea-nuclear Overhauser effect(NOE)experiments using 15N-urea further show no tightly protein-bound urea molecules.Our results thus suggest specific,but weak or transient,urea-protein interactions,supporting the direct interaction model of urea denaturation.