Effects of Probiotics on Breeding Environment and Intestinal Microorganisms of Livestock and Poultry

[Objectives] The paper was to study the effects of probiotics on breeding environment of livestock and poultry and intestinal microorgan-isms of breeding animals. [Method] Chicken houses and hog houses were selected as the research objects. The breeding house supplemented with basal diet was used as the control group;the breeding house sprayed with probiotics product 1 was experimental group 1(exogenous treatment);the basal diet mixed with probiotics product 2 and drinking water mixed with product 3 was experimental group 2(endogenous treatment);and the com-bination of the two experimental groups was experimental group 3. [Results] Experimental groups 1 and 2 reduced the concentration of ammonia in hog houses and chicken houses, and the effect of experimental group 2 was better than that of experimental group 1. Experimental group 3 had the best effect, which significantly reduced the ammonia concentration in hog houses and chicken houses by 28.67% and 34.09%, respectively, and the average ammonia concentration outside the house within the range of 100-300 m was less than 2 mg/m^(3). Moreover, it significantly increased the number of intestinal lactobacillus and reduced the number of Escherichia coli. [Conclusions] Probiotics can effectively reduce the ammonia concentration in livestock and poultry breeding environment, and improve the intestinal microbial structure of breeding animals. The technology of spraying exogenous probiotics combined with the use of content-derived probiotics has great potential for popularization and application.

Influence of Gut Microbiota and its Metabolites on Progression of Metabolic Associated Fatty Liver Disease

Metabolic associated fatty liver disease(MAFLD)has become a prevalent chronic liver disease worldwide because of lifestyle and dietary changes.Gut microbiota and its metabolites have been shown to play a critical role in the pathogenesis of MAFLD.Understanding of the function of gut microbiota and its metabolites in MAFLD may help to elucidate pathological mechanisms,identify diagnostic markers,and develop drugs or probiotics for the treatment of MAFLD.Here we review the pathogenesis of MAFLD by gut microbiota and its metabolites and discuss the feasibility of treating MAFLD from the perspective of gut microbes.

Phasic study of intestinal homeostasis disruption in experimental intestinal obstruction

AIM: To investigate the phasic alteration of intestinal homeostasis in an experimental model of intestinal obstruction.

Simvastatin Improves Outcomes of Endotoxin-induced Coagulopathy by Regulating Intestinal Microenvironment

Objective:The systemic inflammatory response is regarded as the major cause of endotoxin-induced coagulopathy,which is a strong predictor of mortality in patients with severe sepsis.Simvastatin plays an important role in reducing inflammation.In addition,the gut has long been hypothesized to be the“motor”of critical illness,driving or aggravating sepsis by the increased intestinal permeability and bacterial translocation.

Trace metal elements: a bridge between host and intestinal microorganisms

Trace metal elements, such as iron, copper, manganese, and zinc, are essential nutrients for biological processes. Although their intake demand is low, they play a crucial role in cell homeostasis as the cofactors of various enzymes. Symbiotic intestinal microorganisms compete with their host for the use of trace metal elements. Moreover, the metabolic processes of trace metal elements in the host and microorganisms affect the organism's health. Supplementation or the lack of trace metal elements in the host can change the intestinal microbial community structure and function. Functional changes in symbiotic microorganisms can affect the host's metabolism of trace metal elements. In this review, we discuss the absorption and transport processes of trace metal elements in the host and symbiotic microorganisms and the effects of dynamic changes in the levels of trace metal elements on the intestinal microbial community structure. We also highlight the participation of trace metal elements as enzyme cofactors in the host immune process. Our findings indicate that the host uses metal nutrition immunity or metal poisoning to resist pathogens and improve immunity.

Regulation of dietary fiber on intestinal microorganisms and its effects on animal health

The animal gut harbors diverse microbes that play an essential role in the well-being of their host.Specific diets,such as those rich in dietary fiber,are vital in disease prevention and treatment because they affect intestinal flora and have a positive impact on the metabolism,immunity,and intestinal function of the host.Dietary fiber can provide energy to colonic epithelial cells,regulate the structure and metabolism of intestinal flora,promote the production of intestinal mucosa,stimulate intestinal motility,improve glycemic and lipid responses,and regulate the digestion and absorption of nutrients,which is mainly attributed to short-chain fatty acids(SCFA),which is the metabolite of dietary fiber.By binding with G protein-coupled receptors(including GPR41,GPR43 and GPR109A)and inhibiting the activity of histone deacetylases,SCFA regulate appetite and glucolipid metabolism,promote the function of the intestinal barrier,alleviate oxidative stress,suppress inflammation,and maintain immune system homeostasis.This paper reviews the physicochemical properties of dietary fiber,the interaction between dietary fiber and intestinal microorganisms,the role of dietary fiber in maintaining intestinal health,and the function of SCFA,the metabolite of dietary fiber,in inhibiting inflammation.Furthermore,we consider the effects of dietary fiber on the intestinal health of pigs,the reproduction and lactation performance of sows,and the growth performance and meat quality of pigs.

The Use of Restriction Fragment Length Polymorphism and Fluorescence in Situ Hybridization to Investigate Microbiota of Piglets after Feeding Oregano

A total of 80 piglets (7.9 ± 1.0 kg) were used in a feeding experiment with dried oregano. The diets differed in their oregano content: 0 g, 2 g, 4 g and 8 g oregano/kg feed, corresponding to 0, 23.5, 46.9 and 93.9 mg carvacrol/kg DM. After the experimental period of 5 weeks, 20 piglets of both extreme feeding groups were slaughtered: 10 animals of the control group and 10 animals of the group that received 8 g oregano/kg. Ingesta samples of jejunum, caecum and colon were collected and analyzed by FISH and PCR RFLP to compare the diversity of microbiota. The results showed no significant changes in microbiota in response to oregano. The patterns of the PCR-RFLP showed a similarity of 61.8% - 91.8% in both feeding groups. In conclusion, an effect of oregano on the in- testinal microbiota could not be shown under the methods used.

Inhibition of anaerobic probiotics on colorectal cancer cells using intestinal microfluidic systems

Intestinal flora play an important role in human's immune system. Many bacteria adhere to the wall of the testinal wall. These Intestinal flora help digestion, and also stop their disease-causing counterparts from invading. Most of the current researches focused on the interaction between cells and the construction of organs, but few researches studied on the role of microorganisms and cells. Here, we developed an in vitro living cell systems to simulate the structure, absorption, transport and pathophysiological characteristics of the human intestinal tract and the key microbial symbiosis. The co-culture of Clostridium butyricum(C.butyricum) and colon cancer cells showed a different immune effect. C. butyricum could inhibit the proliferation of HCT116 cells, cause cell cycle arrest and promote apoptosis. But it had no significant effect on Caco-2 cells. Thus, basic functional characteristics of the gut were successfully simulated in a controlled microfluidic system. This approach is suggested as a powerful method in the investigation on drug metabolism and intestinal diseases.