Recent studies show that ion channels/transporters play important roles in fundamental cellular functions that would be involved in the cancer process. We review the evidence for their expression and functioning in hu...Recent studies show that ion channels/transporters play important roles in fundamental cellular functions that would be involved in the cancer process. We review the evidence for their expression and functioning in human gastric cancer (GC), and evaluate the potential of cellular physiological approach in clinical management. Various types of ion channels, such as voltage-gated K<sup>+</sup> channels, intracellular Cl<sup>-</sup> channels and transient receptor potential channels have been found to express in GC cells and tissues, and to control cell cycles. With regard to water channels, aquaporin 3 and 5 play an important role in the progression of GC. Regulators of intracellular pH, such as anion exchanger, sodium-hydrogen exchanger, vacuolar H<sup>+</sup>-ATPases and carbonic anhydrases are also involved in tumorigenesis of GC. Their pharmacological manipulation and gene silencing affect cellular behaviours, suggesting their potential as therapeutic targets for GC. Our studies indicate the intracellular Cl<sup>-</sup> concentration could act as a mediator of cellular signaling and control cell cycle progression in GC cells. Further, we demonstrate the cytocidal effects of hypotonic shock on GC cells, and indicate that the blockade of Cl<sup>-</sup> channels/transporters enhances these effects by inhibiting regulatory volume decrease. A deeper understanding of molecular mechanisms may lead to the discovery of these cellular physiological approaches as a novel therapeutic strategy for GC.展开更多
The release of intracellular water during degradation process contributes to the great leachate production and settlement of landfilled high kitchen waste content MSW(HKWC-MSW). An oven-drying and absorbent-paper comb...The release of intracellular water during degradation process contributes to the great leachate production and settlement of landfilled high kitchen waste content MSW(HKWC-MSW). An oven-drying and absorbent-paper combined method was proposed to measure the intracellular and interparticle water contents of HKWC-MSW. Two degradation experiments were carried out to study the release process of intracellular water and its effect on the hydro-mechanical behaviors of HKWC-MSW.It was found that the two degradation experiments showed similar degradation behaviors with BOD/COD decreasing with time in the early stage. In the first degradation experiment, most intracellular water was released during the first two months, and the degradation of degradable matter in kitchen waste(KW) was much slower than the release process of intracellular water. The particle size became smaller and the overall grain specific gravity increased during the fast release process of intracellular water.In the second degradation experiment, after two-year degradation, the total leachate production was about 45.2% of the initial wet weight of HKWC-MSW specimen. Water retention capacity θ_f increased from 0.23 to 0.58 during 1–69 d, which might be caused by the decrease of particle size and compression of waste skeleton. As almost all the intracellular water was released after 80-day degradation, during the latter stage of leachate drainage under gravity, θ_f decreased and was close to the total volumetric water content. The total compression strain was about 0.39. The secondary compression strain during 1–80 d(i.e., about 0.07)was considered to be mainly resulted by the release of intracellular water and the subsequent drainage of leachate, and it accounted for about 22.6% of the total secondary compression strain.展开更多
文摘Recent studies show that ion channels/transporters play important roles in fundamental cellular functions that would be involved in the cancer process. We review the evidence for their expression and functioning in human gastric cancer (GC), and evaluate the potential of cellular physiological approach in clinical management. Various types of ion channels, such as voltage-gated K<sup>+</sup> channels, intracellular Cl<sup>-</sup> channels and transient receptor potential channels have been found to express in GC cells and tissues, and to control cell cycles. With regard to water channels, aquaporin 3 and 5 play an important role in the progression of GC. Regulators of intracellular pH, such as anion exchanger, sodium-hydrogen exchanger, vacuolar H<sup>+</sup>-ATPases and carbonic anhydrases are also involved in tumorigenesis of GC. Their pharmacological manipulation and gene silencing affect cellular behaviours, suggesting their potential as therapeutic targets for GC. Our studies indicate the intracellular Cl<sup>-</sup> concentration could act as a mediator of cellular signaling and control cell cycle progression in GC cells. Further, we demonstrate the cytocidal effects of hypotonic shock on GC cells, and indicate that the blockade of Cl<sup>-</sup> channels/transporters enhances these effects by inhibiting regulatory volume decrease. A deeper understanding of molecular mechanisms may lead to the discovery of these cellular physiological approaches as a novel therapeutic strategy for GC.
基金supported by the National Basic Research Program of China(Grant No.2012CB719800)the National Natural Science Foundation of China(Grant Nos.51708508,41402249)the Zhejiang Provincial Natural Science Foundation(Grant Nos.LY17E080021,LY15E080021)
文摘The release of intracellular water during degradation process contributes to the great leachate production and settlement of landfilled high kitchen waste content MSW(HKWC-MSW). An oven-drying and absorbent-paper combined method was proposed to measure the intracellular and interparticle water contents of HKWC-MSW. Two degradation experiments were carried out to study the release process of intracellular water and its effect on the hydro-mechanical behaviors of HKWC-MSW.It was found that the two degradation experiments showed similar degradation behaviors with BOD/COD decreasing with time in the early stage. In the first degradation experiment, most intracellular water was released during the first two months, and the degradation of degradable matter in kitchen waste(KW) was much slower than the release process of intracellular water. The particle size became smaller and the overall grain specific gravity increased during the fast release process of intracellular water.In the second degradation experiment, after two-year degradation, the total leachate production was about 45.2% of the initial wet weight of HKWC-MSW specimen. Water retention capacity θ_f increased from 0.23 to 0.58 during 1–69 d, which might be caused by the decrease of particle size and compression of waste skeleton. As almost all the intracellular water was released after 80-day degradation, during the latter stage of leachate drainage under gravity, θ_f decreased and was close to the total volumetric water content. The total compression strain was about 0.39. The secondary compression strain during 1–80 d(i.e., about 0.07)was considered to be mainly resulted by the release of intracellular water and the subsequent drainage of leachate, and it accounted for about 22.6% of the total secondary compression strain.
