Intracellular water volume is an important parameter for expressing concentration of intracellular ions and diffusional water permeability of cells by nuclear magnetic resonance(NMR). Presented here, is a method using...Intracellular water volume is an important parameter for expressing concentration of intracellular ions and diffusional water permeability of cells by nuclear magnetic resonance(NMR). Presented here, is a method using 35 Cl and deuterium ( 2D) NMR spectra of cell free supernatant and cell suspension to get intracellular water volume. The volume of intracellular water as a fraction of the total cell volume, V i /V c , in isosmotic solution is 0 706±0 065.展开更多
With the aim of studying the effect of water dynamics on the properties of biological systems, in this paper, we present a quasi-elastic neutron scattering study on three different types of living cells, differing bot...With the aim of studying the effect of water dynamics on the properties of biological systems, in this paper, we present a quasi-elastic neutron scattering study on three different types of living cells, differing both in their morphological and tumor properties. The measured scattering signal, which essentially originates from hydrogen atoms present in the investigated systems, has been analyzed using a global fitting strategy using an optimized theoretical model that considers various classes of hydrogen atoms and allows disentangling diffusive and rotational motions. The approach has been carefully validated by checking the reliability of the calculation of parameters and their 99% confidence intervals. We demonstrate that quasi-elastic neutron scattering is a suitable experimental technique to characterize the dynamics of intracellular water in the angstrom/picosecond space/time scale and to investigate the effect of water dynamics on cellular biodiversity.展开更多
This study aims to increase the inactivation efficiency of CO_2 against Escherichia coli under mild conditions to facilitate the application of pressurized CO_2 technology in water disinfection. Based on an aerating-c...This study aims to increase the inactivation efficiency of CO_2 against Escherichia coli under mild conditions to facilitate the application of pressurized CO_2 technology in water disinfection. Based on an aerating-cycling apparatus, three different treatment methods（continuous aeration, continuous reflux, and simultaneous aeration and reflux） were compared for the same temperature, pressure（0.3–0.7 MPa）, initial concentration, and exposure time（25 min）. The simultaneous aeration and reflux treatment（combined method） was shown to be the best method under optimum conditions, which were determined to be 0.7 MPa, room temperature, and an exposure time of 10 min. This treatment achieved 5.1-log reduction after 25 min of treatment at the pressure of 0.3 MPa and 5.73-log reduction after 10 min at 0.7 MPa. Log reductions of 4.4 and 5.0 occurred at the end of continuous aeration and continuous reflux treatments at 0.7 MPa, respectively.Scanning electron microscopy（SEM） images suggested that cells were ruptured after the simultaneous aeration and reflux treatment and the continuous reflux treatment. The increase of the solubilization rate of CO_2 due to intense hydraulic conditions led to a rapid inactivation effect. It was found that the reduction of intracellular p H caused by CO_2 led to a more lethal bactericidal effect.展开更多
文摘Intracellular water volume is an important parameter for expressing concentration of intracellular ions and diffusional water permeability of cells by nuclear magnetic resonance(NMR). Presented here, is a method using 35 Cl and deuterium ( 2D) NMR spectra of cell free supernatant and cell suspension to get intracellular water volume. The volume of intracellular water as a fraction of the total cell volume, V i /V c , in isosmotic solution is 0 706±0 065.
文摘With the aim of studying the effect of water dynamics on the properties of biological systems, in this paper, we present a quasi-elastic neutron scattering study on three different types of living cells, differing both in their morphological and tumor properties. The measured scattering signal, which essentially originates from hydrogen atoms present in the investigated systems, has been analyzed using a global fitting strategy using an optimized theoretical model that considers various classes of hydrogen atoms and allows disentangling diffusive and rotational motions. The approach has been carefully validated by checking the reliability of the calculation of parameters and their 99% confidence intervals. We demonstrate that quasi-elastic neutron scattering is a suitable experimental technique to characterize the dynamics of intracellular water in the angstrom/picosecond space/time scale and to investigate the effect of water dynamics on cellular biodiversity.
基金supported by the National Natural Science Foundation of China (Nos. 51178323, 51108329, 51378369)
文摘This study aims to increase the inactivation efficiency of CO_2 against Escherichia coli under mild conditions to facilitate the application of pressurized CO_2 technology in water disinfection. Based on an aerating-cycling apparatus, three different treatment methods（continuous aeration, continuous reflux, and simultaneous aeration and reflux） were compared for the same temperature, pressure（0.3–0.7 MPa）, initial concentration, and exposure time（25 min）. The simultaneous aeration and reflux treatment（combined method） was shown to be the best method under optimum conditions, which were determined to be 0.7 MPa, room temperature, and an exposure time of 10 min. This treatment achieved 5.1-log reduction after 25 min of treatment at the pressure of 0.3 MPa and 5.73-log reduction after 10 min at 0.7 MPa. Log reductions of 4.4 and 5.0 occurred at the end of continuous aeration and continuous reflux treatments at 0.7 MPa, respectively.Scanning electron microscopy（SEM） images suggested that cells were ruptured after the simultaneous aeration and reflux treatment and the continuous reflux treatment. The increase of the solubilization rate of CO_2 due to intense hydraulic conditions led to a rapid inactivation effect. It was found that the reduction of intracellular p H caused by CO_2 led to a more lethal bactericidal effect.
