Intraoperative blood salvage autotransfusion (IBSA) is used in various surgical procedures. However, because of the risk of reinfusion of salvaged blood contaminated by tumor cells, the use of IBSA in hepatocellular c...Intraoperative blood salvage autotransfusion (IBSA) is used in various surgical procedures. However, because of the risk of reinfusion of salvaged blood contaminated by tumor cells, the use of IBSA in hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) is controversial. The critical points include whether tumor cells can be cleared by IBSA, whether IBSA increases the risk of recurrence or metastasis, and what are the indications for IBSA. Moreover, is it warranted to take the risk of tumor dissemination by using IBSA to avoid allogeneic blood transfusion? Do the remaining tumor cells after additional filtration by leukocyte depletion filters still possess potential tumorigenicity? Does IBSA always work well? We have reviewed the literature and tried to address these questions. The available data indicate that IBSA is safe in LT for HCC, but randomized, controlled and prospective trials are urgently required to clarify the uncertainty.展开更多
Blood loss during liver transplantation (OLTx) is a common consequence of pre-existing abnormalities of the hemostatic system,portal hypertension with multiple collateral vessels,portal vein thrombosis,previous abdomi...Blood loss during liver transplantation (OLTx) is a common consequence of pre-existing abnormalities of the hemostatic system,portal hypertension with multiple collateral vessels,portal vein thrombosis,previous abdominal surgery,splenomegaly,and poor "functional" recovery of the new liver.The intrinsic coagulopathic features of end stage cirrhosis along with surgical technical difficulties make transfusion-free liver transplantation a major challenge,and,despite the improvements in understanding of intraoperative coagulation profiles and strategies to control blood loss,the requirements for blood or blood products remains high.The impact of blood transfusion has been shown to be significant and independent of other well-known predictors of posttransplant-outcome.Negative effects on immunomodulation and an increased risk of postoperative complications and mortality have been repeatedly demonstrated.Isovolemic hemodilution,the extensive utilization of thromboelastogram and the use of autotransfusion devices are among the commonly adopted procedures to limit the amount of blood transfusion.The use of intraoperative blood salvage and autologous blood transfusion should still be considered an important method to reduce the need for allogenic blood and the associated complications.In this article we report on the common preoperative and intraoperative factors contributing to blood loss,intraoperative transfusion practices,anesthesiologic and surgical strategies to prevent blood loss,and on intraoperative blood salvaging techniques and autologous blood transfusion.Even though the advances in surgical technique and anesthetic management,as well as a better understanding of the risk factors,have resulted in a steady decrease in intraoperative bleeding,most patients still bleed extensively.Blood transfusion therapy is still a critical feature during OLTx and various studies have shown a large variability in the use of blood products among different centers and even among individual anesthesiologists within the same center.Unfortunately,despite the large number of OLTx performed each year,there is still paucity of large randomized,multicentre,and controlled studies which indicate how to prevent bleeding,the transfusion needs and thresholds,and the "evidence based" perioperative strategies to reduce the amount of transfusion.展开更多
目的荟萃分析回收式自体输血(IOCS)在前置胎盘剖宫产术中的安全性和有效性。方法计算机检索PubMed、Web of Science、Embase、the Cochrane Library of clinical trials、中国知网(CNKI)及万方数据知识服务平台。检索时限设定为建库至2...目的荟萃分析回收式自体输血(IOCS)在前置胎盘剖宫产术中的安全性和有效性。方法计算机检索PubMed、Web of Science、Embase、the Cochrane Library of clinical trials、中国知网(CNKI)及万方数据知识服务平台。检索时限设定为建库至2022年12月。采用R 4.1.2与Stata 12.0软件计算IOCS组和异体输血(ABT)组之间的标准化均数差(SMD)或相对危险度(RR)及95%置信区间(CI)和预测区间(PI)。结果本次Meta分析共纳入5项随机对照试验和10项回顾性队列研究。队列研究的合并结果显示,与ABT组相比,接受IOCS的前置胎盘孕产妇术后血红蛋白(Hb)浓度(SMD=0.626,95%CI:0.103~1.149;95%PI:-1.320~2.572)与红细胞压积较高(SMD=0.617,95%CI:0.130~1.104;95%PI:-1.084~2.317)。在随机对照试验中,接受IOCS的前置胎盘孕产妇发生不良事件的风险比ABT组低72.7%(RR=0.273,95%CI:0.082~0.904)。IOCS组和ABT组术后凝血酶原时间(PT)、活化凝血酶原时间(APTT)、纤维蛋白原(Fib)浓度、血尿素氮(BUN)和肌酐(Cr)差异均无无统计学意义。结论接受IOCS的前置胎盘孕产妇术后Hb浓度和Hct均高于接受ABT的妇女。IOCS对术后凝血参数和肾功能参数无显著影响。在接受剖宫产术的前置胎盘孕产妇中,IOCS与较低的输血相关不良事件发生风险有关。展开更多
Background Recurarization has previously been described in the context of acute normovolemic hemodilution.The aim of this study was to investigate the impairment of recovery of neuromuscular function after re-transfus...Background Recurarization has previously been described in the context of acute normovolemic hemodilution.The aim of this study was to investigate the impairment of recovery of neuromuscular function after re-transfusion of intraoperative salvaged blood in patients treated with rocuronium.Methods We enrolled 50 patients undergoing general anesthesia for lumbar surgery.Intraoperative blood salvage （IBS） was used in 30 patients （group Ⅰ）; the remaining 20 comprised a control group （group C）.Anesthesia was induced with fentanyl,midazolam,propofol and rocuronium.Rocuronium was infused to maintain neuromuscular blockade during surgery.Blood was collected from the operative field and re-transfused in the post-anesthesia care unit （PACU）.Neuromuscular function was monitored using the train-of-four ratio （TOFr）.Once the train-of-four ratio exceeded 90 in the PACU,neuromuscular function was evaluated every 5 minutes for 30 minutes.The TOFr and incremental recovery of TOFr from baseline were recorded.Salvaged blood was re-transfused at the beginning of the evaluation for patients in group Ⅰ,and afterwards for patients in group C.Blood gas analysis was assessed before anesthesia and in the PACU.Results Incremental recovery of TOFr from baseline was significantly less in group Ⅰ than controls at 25 minutes （6.1±3.2vs.9.1±3.2,respectively; P=0.001） and 30 minutes （7.1±3.2 vs.10.0±2.2,respectively; P=-0.001）.There were no significant differences in gas exchange between the groups.Conclusions In patients who had received a rocuronium infusion during anesthesia,re-transfusion of salvaged blood significantly impaired recovery of neuromuscular function recovery in the PACU,but without significant impairment of respiratory function.展开更多
The safe use of intraoperative blood salvage （IBS） in cancer surgery remains controversial. Here, we investigated the kil ing effect of cisplatin combined with hyperthermia on human hepatocarcinoma （HepG2） cel s a...The safe use of intraoperative blood salvage （IBS） in cancer surgery remains controversial. Here, we investigated the kil ing effect of cisplatin combined with hyperthermia on human hepatocarcinoma （HepG2） cel s and erythrocytes from IBS in vitro. HepG2 cel s were mixed with concentrated erythrocytes and pretreated with cisplatin （50, 100, and 200μg/ml） alone at 37 °C for 60 min and cisplatin （25, 50, 100, and 200μg/ml） combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cel viability, colony formation and DNA metabolism in HepG2 and the Na＋-K＋-ATPase activity, 2,3-diphosphoglycerate （2,3-DPG） concentration, free hemoglobin （Hb） level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin （50, 100, and 200μg/ml） combined with hyperthermia （42 °C） for 60 min significantly decreased HepG2 cel viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cel concentration was 5×104 ml？1 in the erythrocyte （P0.05）. In conclusion, pre-treatment with cisplatin （50μg/ml） combined with hyperthermia （42 °C） for 60 min effectively eliminated HepG2 cel s from IBS but did not significantly affect erythrocytes in vitro.展开更多
目的:研究贮存式自体血输注(preoperative autologous blood donation,PABD)在普外科择期手术中的临床应用效果。方法:筛选2017年11月-2018年8月本院普外科贮存式自体血输注70例,采用配对研究方法,将未采用贮存式自体血输注且术前基线...目的:研究贮存式自体血输注(preoperative autologous blood donation,PABD)在普外科择期手术中的临床应用效果。方法:筛选2017年11月-2018年8月本院普外科贮存式自体血输注70例,采用配对研究方法,将未采用贮存式自体血输注且术前基线资料无统计学差异的70例患者,配对作为对照组。比较2组患者异体红细胞和血浆输注量,围术期Hb和Plt变化,住院时间及住院费用的差异。结果:PABD组采血前、后Hb和Plt分别为138.26±14.73 vs 127.52±13.36g/L(P<0.05)和(221.67±52.86 vs 198.35±52.65)×10^9/L(P>0.05);2组患者围术期异体红细胞和血浆的输注量分别为0.20±0.71 vs 0.89±0.97 U和30.43±100.81 vs 106.52±152.61 ml(P<0.05);2组患者术前Hb水平135.65±14.16 vs 134.15±11.98 g/L,术前Plt水平(270.36±58.28 vs 271.67±65.02)×10^9/L;术后1 d Hb水平120.24±14.40 vs 121.20±14.30 g/L,术后1 d Plt水平(241.80±63.58 vs 241.30±69.11)×10^9/L;术后3 d Hb水平123.15±13.80 vs 121.65±14.33 g/L,术后3 d Plt水平(251.26±72.94 vs 255.54±73.85)×10^9/L;出院前Hb水平122.78±13.92 vs 122.00±13.82 g/L,出院前Plt(262.50±80.96 vs 264.56±71.08)×10^9/L(P>0.05);2组患者的住院时间14.84±3.37 vs 14.84±2.24(d)(P>0.05),住院总费用和输血相关费用分别为50627.27±9889.45 vs 50979.43±8195.00元和354.39±362.57 vs 684.02±425.53元(P<0.05)。结论:择期手术估计术中失血量1000ml的患者,贮存式自体血输注的应用可节约异体血输注,降低费用。展开更多
目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资...目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资料、手术前后Hb和Plt、住院时间、术中出血量、术中自体血回收量、术前自体浓缩红细胞和血小板采集量、术中异体红细胞、血浆及血小板的输注量。结果 1)2组患者基线水平一致,手术前后Hb和Plt、住院时间均无统计学差异(P>0.05);2)深度自体组和对照组患者术中出血量分别为2 000 mL(1 200,3 500)vs 2 600 mL(1 000,3 875)(P>0.05),术中自体血回收量为44 U vs 9 U;3)2组术前自体浓缩红细胞和自体血小板采集总量分别为68 U vs 4 U、17 U vs 0 U;4)2组围手术期异体红细胞、血浆和血小板的输注总量分别为18 U vs 53 U、24 U vs 66 U、2 U vs 4 U;5)深度自体组有5例患者避免了异体血输注,占55.6%(5/9)。结论单采深度自体储血技术能在短时间内获得大量的自体红细胞和自体血小板,具备避免特大择期手术患者使用异体红细胞和异体血小板的巨大潜力。展开更多
文摘Intraoperative blood salvage autotransfusion (IBSA) is used in various surgical procedures. However, because of the risk of reinfusion of salvaged blood contaminated by tumor cells, the use of IBSA in hepatocellular carcinoma (HCC) patients undergoing liver transplantation (LT) is controversial. The critical points include whether tumor cells can be cleared by IBSA, whether IBSA increases the risk of recurrence or metastasis, and what are the indications for IBSA. Moreover, is it warranted to take the risk of tumor dissemination by using IBSA to avoid allogeneic blood transfusion? Do the remaining tumor cells after additional filtration by leukocyte depletion filters still possess potential tumorigenicity? Does IBSA always work well? We have reviewed the literature and tried to address these questions. The available data indicate that IBSA is safe in LT for HCC, but randomized, controlled and prospective trials are urgently required to clarify the uncertainty.
文摘Blood loss during liver transplantation (OLTx) is a common consequence of pre-existing abnormalities of the hemostatic system,portal hypertension with multiple collateral vessels,portal vein thrombosis,previous abdominal surgery,splenomegaly,and poor "functional" recovery of the new liver.The intrinsic coagulopathic features of end stage cirrhosis along with surgical technical difficulties make transfusion-free liver transplantation a major challenge,and,despite the improvements in understanding of intraoperative coagulation profiles and strategies to control blood loss,the requirements for blood or blood products remains high.The impact of blood transfusion has been shown to be significant and independent of other well-known predictors of posttransplant-outcome.Negative effects on immunomodulation and an increased risk of postoperative complications and mortality have been repeatedly demonstrated.Isovolemic hemodilution,the extensive utilization of thromboelastogram and the use of autotransfusion devices are among the commonly adopted procedures to limit the amount of blood transfusion.The use of intraoperative blood salvage and autologous blood transfusion should still be considered an important method to reduce the need for allogenic blood and the associated complications.In this article we report on the common preoperative and intraoperative factors contributing to blood loss,intraoperative transfusion practices,anesthesiologic and surgical strategies to prevent blood loss,and on intraoperative blood salvaging techniques and autologous blood transfusion.Even though the advances in surgical technique and anesthetic management,as well as a better understanding of the risk factors,have resulted in a steady decrease in intraoperative bleeding,most patients still bleed extensively.Blood transfusion therapy is still a critical feature during OLTx and various studies have shown a large variability in the use of blood products among different centers and even among individual anesthesiologists within the same center.Unfortunately,despite the large number of OLTx performed each year,there is still paucity of large randomized,multicentre,and controlled studies which indicate how to prevent bleeding,the transfusion needs and thresholds,and the "evidence based" perioperative strategies to reduce the amount of transfusion.
文摘目的荟萃分析回收式自体输血(IOCS)在前置胎盘剖宫产术中的安全性和有效性。方法计算机检索PubMed、Web of Science、Embase、the Cochrane Library of clinical trials、中国知网(CNKI)及万方数据知识服务平台。检索时限设定为建库至2022年12月。采用R 4.1.2与Stata 12.0软件计算IOCS组和异体输血(ABT)组之间的标准化均数差(SMD)或相对危险度(RR)及95%置信区间(CI)和预测区间(PI)。结果本次Meta分析共纳入5项随机对照试验和10项回顾性队列研究。队列研究的合并结果显示,与ABT组相比,接受IOCS的前置胎盘孕产妇术后血红蛋白(Hb)浓度(SMD=0.626,95%CI:0.103~1.149;95%PI:-1.320~2.572)与红细胞压积较高(SMD=0.617,95%CI:0.130~1.104;95%PI:-1.084~2.317)。在随机对照试验中,接受IOCS的前置胎盘孕产妇发生不良事件的风险比ABT组低72.7%(RR=0.273,95%CI:0.082~0.904)。IOCS组和ABT组术后凝血酶原时间(PT)、活化凝血酶原时间(APTT)、纤维蛋白原(Fib)浓度、血尿素氮(BUN)和肌酐(Cr)差异均无无统计学意义。结论接受IOCS的前置胎盘孕产妇术后Hb浓度和Hct均高于接受ABT的妇女。IOCS对术后凝血参数和肾功能参数无显著影响。在接受剖宫产术的前置胎盘孕产妇中,IOCS与较低的输血相关不良事件发生风险有关。
文摘Background Recurarization has previously been described in the context of acute normovolemic hemodilution.The aim of this study was to investigate the impairment of recovery of neuromuscular function after re-transfusion of intraoperative salvaged blood in patients treated with rocuronium.Methods We enrolled 50 patients undergoing general anesthesia for lumbar surgery.Intraoperative blood salvage （IBS） was used in 30 patients （group Ⅰ）; the remaining 20 comprised a control group （group C）.Anesthesia was induced with fentanyl,midazolam,propofol and rocuronium.Rocuronium was infused to maintain neuromuscular blockade during surgery.Blood was collected from the operative field and re-transfused in the post-anesthesia care unit （PACU）.Neuromuscular function was monitored using the train-of-four ratio （TOFr）.Once the train-of-four ratio exceeded 90 in the PACU,neuromuscular function was evaluated every 5 minutes for 30 minutes.The TOFr and incremental recovery of TOFr from baseline were recorded.Salvaged blood was re-transfused at the beginning of the evaluation for patients in group Ⅰ,and afterwards for patients in group C.Blood gas analysis was assessed before anesthesia and in the PACU.Results Incremental recovery of TOFr from baseline was significantly less in group Ⅰ than controls at 25 minutes （6.1±3.2vs.9.1±3.2,respectively; P=0.001） and 30 minutes （7.1±3.2 vs.10.0±2.2,respectively; P=-0.001）.There were no significant differences in gas exchange between the groups.Conclusions In patients who had received a rocuronium infusion during anesthesia,re-transfusion of salvaged blood significantly impaired recovery of neuromuscular function recovery in the PACU,but without significant impairment of respiratory function.
基金Project supported by the Scientific Research from Chinese Ministryof Health-Zhejiang Health Department,China(Nos.WKJ2008-2-021and WKJ2013-2-019)
文摘The safe use of intraoperative blood salvage （IBS） in cancer surgery remains controversial. Here, we investigated the kil ing effect of cisplatin combined with hyperthermia on human hepatocarcinoma （HepG2） cel s and erythrocytes from IBS in vitro. HepG2 cel s were mixed with concentrated erythrocytes and pretreated with cisplatin （50, 100, and 200μg/ml） alone at 37 °C for 60 min and cisplatin （25, 50, 100, and 200μg/ml） combined with hyperthermia at 42 °C for 60 min. After pretreatment, the cel viability, colony formation and DNA metabolism in HepG2 and the Na＋-K＋-ATPase activity, 2,3-diphosphoglycerate （2,3-DPG） concentration, free hemoglobin （Hb） level, osmotic fragility, membrane phosphatidylserine externalization, and blood gas variables in erythrocytes were determined. Pretreatment with cisplatin （50, 100, and 200μg/ml） combined with hyperthermia （42 °C） for 60 min significantly decreased HepG2 cel viability, and completely inhibited colony formation and DNA metabolism when the HepG2 cel concentration was 5×104 ml？1 in the erythrocyte （P0.05）. In conclusion, pre-treatment with cisplatin （50μg/ml） combined with hyperthermia （42 °C） for 60 min effectively eliminated HepG2 cel s from IBS but did not significantly affect erythrocytes in vitro.
文摘目的:研究贮存式自体血输注(preoperative autologous blood donation,PABD)在普外科择期手术中的临床应用效果。方法:筛选2017年11月-2018年8月本院普外科贮存式自体血输注70例,采用配对研究方法,将未采用贮存式自体血输注且术前基线资料无统计学差异的70例患者,配对作为对照组。比较2组患者异体红细胞和血浆输注量,围术期Hb和Plt变化,住院时间及住院费用的差异。结果:PABD组采血前、后Hb和Plt分别为138.26±14.73 vs 127.52±13.36g/L(P<0.05)和(221.67±52.86 vs 198.35±52.65)×10^9/L(P>0.05);2组患者围术期异体红细胞和血浆的输注量分别为0.20±0.71 vs 0.89±0.97 U和30.43±100.81 vs 106.52±152.61 ml(P<0.05);2组患者术前Hb水平135.65±14.16 vs 134.15±11.98 g/L,术前Plt水平(270.36±58.28 vs 271.67±65.02)×10^9/L;术后1 d Hb水平120.24±14.40 vs 121.20±14.30 g/L,术后1 d Plt水平(241.80±63.58 vs 241.30±69.11)×10^9/L;术后3 d Hb水平123.15±13.80 vs 121.65±14.33 g/L,术后3 d Plt水平(251.26±72.94 vs 255.54±73.85)×10^9/L;出院前Hb水平122.78±13.92 vs 122.00±13.82 g/L,出院前Plt(262.50±80.96 vs 264.56±71.08)×10^9/L(P>0.05);2组患者的住院时间14.84±3.37 vs 14.84±2.24(d)(P>0.05),住院总费用和输血相关费用分别为50627.27±9889.45 vs 50979.43±8195.00元和354.39±362.57 vs 684.02±425.53元(P<0.05)。结论:择期手术估计术中失血量1000ml的患者,贮存式自体血输注的应用可节约异体血输注,降低费用。
文摘目的探讨单采深度自体储血技术(advanced autologous apheresis,AAA)在择期特大手术中的节血效果。方法选择本院2012年1月-2015年12月行巨大神经纤维瘤择期手术的15例患者,根据是否进行AAA分为深度自体组和对照组,比较2组患者的基本资料、手术前后Hb和Plt、住院时间、术中出血量、术中自体血回收量、术前自体浓缩红细胞和血小板采集量、术中异体红细胞、血浆及血小板的输注量。结果 1)2组患者基线水平一致,手术前后Hb和Plt、住院时间均无统计学差异(P>0.05);2)深度自体组和对照组患者术中出血量分别为2 000 mL(1 200,3 500)vs 2 600 mL(1 000,3 875)(P>0.05),术中自体血回收量为44 U vs 9 U;3)2组术前自体浓缩红细胞和自体血小板采集总量分别为68 U vs 4 U、17 U vs 0 U;4)2组围手术期异体红细胞、血浆和血小板的输注总量分别为18 U vs 53 U、24 U vs 66 U、2 U vs 4 U;5)深度自体组有5例患者避免了异体血输注,占55.6%(5/9)。结论单采深度自体储血技术能在短时间内获得大量的自体红细胞和自体血小板,具备避免特大择期手术患者使用异体红细胞和异体血小板的巨大潜力。
