Inulin-type fructan CP-A,a predominant polysaccharide in Codonopsis pilosula,demonstrates regulatory effects on immune activity and anti-inflammation.The efficacy of CP-A in treating ulcerative colitis(UC)is,however,n...Inulin-type fructan CP-A,a predominant polysaccharide in Codonopsis pilosula,demonstrates regulatory effects on immune activity and anti-inflammation.The efficacy of CP-A in treating ulcerative colitis(UC)is,however,not well-established.This study employed an in vitro lipopolysaccharide(LPS)-induced colonic epithelial cell model(NCM460)and an in vivo dextran sulfate sodium(DSS)-induced colitis mouse model to explore CP-A’s protective effects against experimental colitis and its underlying mechanisms.We monitored the clinical symptoms in mice using various parameters:body weight,disease activity index(DAI),colon length,spleen weight,and histopathological scores.Additionally,molecular markers were assessed through enzyme-linked immunosorbent assay(ELISA),quantitative real-time polymerase chain reaction(qRT-PCR),immunofluorescence(IF),immunohistochemistry(IHC),and Western blotting assays.Results showed that CP-A significantly reduced reactive oxygen species(ROS),tumor necrosis factor-alpha(TNF-α),and interleukins(IL-6,IL-1β,IL-18)in LPS-induced cells while increasing IL-4 and IL-10 levels and enhancing the expression of Claudin-1,ZO-1,and occludin proteins in NCM460 cells.Correspondingly,in vivo findings revealed that CPA administration markedly improved DAI,reduced colon shortening,and decreased the production of myeloperoxidase(MPO),malondialdehyde(MDA),ROS,IL-1β,IL-18,and NOD-like receptor protein 3(NLRP3)inflammasome-associated genes/proteins in UC mice.CP-A treatment also elevated glutathione(GSH)and superoxide dismutase(SOD)levels,stimulated autophagy(LC3B,P62,Beclin-1,and ATG5),and reinforced Claudin-1 and ZO-1 expression,thereby aiding in intestinal epithelial barrier repair in colitis mice.Notably,the inhibition of autophagy via chloroquine(CQ)diminished CP-A’s protective impact against colitis in vivo.These findings elucidate that CP-A’s therapeutic effect on experimental colitis possibly involves mitigating intestinal inflammation through autophagymediated NLRP3 inflammasome inactivation.Consequently,inulin-type fructan CP-A emerges as a promising drug candidate for UC treatment.展开更多
Vegan consumers look for fresh,tasty,and nutritious products that use eco/animal-friendly ingredients.Prebiotics are substrates selectively utilized by host microorganisms conferring a health benefit.This study aimed ...Vegan consumers look for fresh,tasty,and nutritious products that use eco/animal-friendly ingredients.Prebiotics are substrates selectively utilized by host microorganisms conferring a health benefit.This study aimed to overview the potentially vegan products added with prebiotics,their health benefits,and the main technological and sensory challenges.The main potentially vegan products added with prebiotics studied were based on water-soluble plant-based extracts(fermented beverages,frozen desserts,cheese analog),fruits(fruit juices,fruit jelly,and ready to eat fruits),and cereals(snack bars,granola bars,bread,and cookies).The main prebiotic compounds used were inulin,fructooligosaccharides,oligofructose,polydextrose,and xylooligosaccharides.The effects of prebiotic on food products’,host health,and technological and sensory properties depended on the prebiotic type and dosage,food matrix,and subject type.The positive health effects were related to skin health,gut microbiota improvement,hypocholesterolemic impact,reduction of the risk of metabolic diseases,and improvements in postprandial blood glucose and insulin response.Prebiotics could improve vegan products’technological and sensory properties,such as fermentation time,texture and rheological properties,storage length,sensory acceptance,and probiotic survival.Furthermore,they could increase the concentration and/or bioaccessibility of phenolic compounds and vitamins.However,it is of paramount importance the determination of prebiotic stability.The trends direct to new prebiotic compounds based on microalgae,fruit and cereal by-products,and seeds.This review provides industries and researchers with important information about prebiotics and vegan products.展开更多
基金supported by the National Natural Science Foundation of China(No.81904031)National Key Research and Development Program of China(No.2019YFC1710800)+1 种基金the Natural Science Foundation of Shanxi Province(No.201901D211325)the Science Research Start-up Fund for Doctor of Shanxi Medical University(No.XD1802).
文摘Inulin-type fructan CP-A,a predominant polysaccharide in Codonopsis pilosula,demonstrates regulatory effects on immune activity and anti-inflammation.The efficacy of CP-A in treating ulcerative colitis(UC)is,however,not well-established.This study employed an in vitro lipopolysaccharide(LPS)-induced colonic epithelial cell model(NCM460)and an in vivo dextran sulfate sodium(DSS)-induced colitis mouse model to explore CP-A’s protective effects against experimental colitis and its underlying mechanisms.We monitored the clinical symptoms in mice using various parameters:body weight,disease activity index(DAI),colon length,spleen weight,and histopathological scores.Additionally,molecular markers were assessed through enzyme-linked immunosorbent assay(ELISA),quantitative real-time polymerase chain reaction(qRT-PCR),immunofluorescence(IF),immunohistochemistry(IHC),and Western blotting assays.Results showed that CP-A significantly reduced reactive oxygen species(ROS),tumor necrosis factor-alpha(TNF-α),and interleukins(IL-6,IL-1β,IL-18)in LPS-induced cells while increasing IL-4 and IL-10 levels and enhancing the expression of Claudin-1,ZO-1,and occludin proteins in NCM460 cells.Correspondingly,in vivo findings revealed that CPA administration markedly improved DAI,reduced colon shortening,and decreased the production of myeloperoxidase(MPO),malondialdehyde(MDA),ROS,IL-1β,IL-18,and NOD-like receptor protein 3(NLRP3)inflammasome-associated genes/proteins in UC mice.CP-A treatment also elevated glutathione(GSH)and superoxide dismutase(SOD)levels,stimulated autophagy(LC3B,P62,Beclin-1,and ATG5),and reinforced Claudin-1 and ZO-1 expression,thereby aiding in intestinal epithelial barrier repair in colitis mice.Notably,the inhibition of autophagy via chloroquine(CQ)diminished CP-A’s protective impact against colitis in vivo.These findings elucidate that CP-A’s therapeutic effect on experimental colitis possibly involves mitigating intestinal inflammation through autophagymediated NLRP3 inflammasome inactivation.Consequently,inulin-type fructan CP-A emerges as a promising drug candidate for UC treatment.
基金This work was supported by the“Coordenaç˜ao de Aperfeiçoamento de Pessoal de Nível Superior”(CAPE,Brazil,Finance code 001)Fundaç˜ao de Apoio a Pesquisa do estado da Paraíba(Fapesq,Brazil 4/2021)“Conselho Nacional de Desenvolvimento Científico e Tecnol´ogico”(CNPq,Brazil,Process number 304075/2019-1).
文摘Vegan consumers look for fresh,tasty,and nutritious products that use eco/animal-friendly ingredients.Prebiotics are substrates selectively utilized by host microorganisms conferring a health benefit.This study aimed to overview the potentially vegan products added with prebiotics,their health benefits,and the main technological and sensory challenges.The main potentially vegan products added with prebiotics studied were based on water-soluble plant-based extracts(fermented beverages,frozen desserts,cheese analog),fruits(fruit juices,fruit jelly,and ready to eat fruits),and cereals(snack bars,granola bars,bread,and cookies).The main prebiotic compounds used were inulin,fructooligosaccharides,oligofructose,polydextrose,and xylooligosaccharides.The effects of prebiotic on food products’,host health,and technological and sensory properties depended on the prebiotic type and dosage,food matrix,and subject type.The positive health effects were related to skin health,gut microbiota improvement,hypocholesterolemic impact,reduction of the risk of metabolic diseases,and improvements in postprandial blood glucose and insulin response.Prebiotics could improve vegan products’technological and sensory properties,such as fermentation time,texture and rheological properties,storage length,sensory acceptance,and probiotic survival.Furthermore,they could increase the concentration and/or bioaccessibility of phenolic compounds and vitamins.However,it is of paramount importance the determination of prebiotic stability.The trends direct to new prebiotic compounds based on microalgae,fruit and cereal by-products,and seeds.This review provides industries and researchers with important information about prebiotics and vegan products.
