Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 ...Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.展开更多
当前基于三维点云的工业异常检测仍处于起步阶段,较先进的方法将点云栅格化成2D图片或采用传统算子提取点云特征,有待进一步研究。对此,提出一种基于3D归一化流的双分支点云异常检测模型。以3D归一化流作为整体框架,结合双分支交互融合...当前基于三维点云的工业异常检测仍处于起步阶段,较先进的方法将点云栅格化成2D图片或采用传统算子提取点云特征,有待进一步研究。对此,提出一种基于3D归一化流的双分支点云异常检测模型。以3D归一化流作为整体框架,结合双分支交互融合模块进行局部特征聚合,以并行结构聚合由Transformer和卷积神经网络(Convs)架构提取的不同焦点的点云特征;通过3D归一化流对特征金字塔在欧氏空间和潜在空间之间的可逆映射进行建模,基于受试者曲线下面积,所提模型从分布学习的角度实现了图像级(I-AUROC)的高效异常检测。在MVTec-3D AD数据集上进行的实验结果表明,所提模型在点云异常检测中取得了比目前先进方法更好的性能,相较于M3DM(Multi-3D-Memory)、AST(Asymmetric Student-Teacher Networks),I-AUROC(Image-level Area Under the Receiver Operating Characteristiccurve)指标分别提升了1.8和5.9个百分点。展开更多
Complex dynamical phenomenon was studied in the single phase H-bridge inverter which was controlled by either a peak current or a valley current. The state functions and the discrete iterative map equations were estab...Complex dynamical phenomenon was studied in the single phase H-bridge inverter which was controlled by either a peak current or a valley current. The state functions and the discrete iterative map equations were established to analyze the dynamical phenomenon in the single phase H-bridge inverter. The dynamical characteristics of the single phase H- bridge inverter, such as time domain waveform diagram, bifurcation diagram, and folding map, were obtained by using the numerical calculation when the circuit parameters varied in specific range. Moreover, the simulation results were obtained by using the OrCAD-PSpice software to validate the numerical calculation. Both the numerical calculation and the circuit simulation show that the symmetrical dynamical phenomenon occurs in the single phase H-bridge inverter controlled by the peak current or the valley current.展开更多
文摘Objective\ To understand the transcription of BamHI L DNA fragment from genome of strong virulent GA strain of Marek′s disease herpesvirus (MDV) in lymphoblastoid tumor tissue induced by oncogenic strain Beijing 1 (a specific local strain in China) of MDV. Methods\ Two oligonucleotide primers were synthesized according to the reported sequence of \%meq\% gene an ideal oncogenic candidate and our previously determined sequence of BamHI L fragment of Marek′s disease herpesvirus (MDV), respectively. Reverse transcriptase PCR(RT PCR) assay was performed by using these primers and the mRNA as a template which was isolated from visceral lymphoblastoid tumors obtained from chickens artificially infected with strain Beijing 1 of oncogenic MDV. Southern blot molecular hybridization was further carried out to detect the product of RT PCR with digoxigenin labeled nucleotide probe from BamHI I2 and L fragment in the gene library of MDV strain GA, respectively. Results\ Two probes could simultaneously hybridize this cDNA amplified by RT PCR with a length of about 730 bp. Conclusion\ It is suggested that \%meq\% transcription could extend from the right hand end of BamHI I2 to the adjacent BamHI L, and the BamHI L region was likely to be transcribed in MDV induced lymphoblastoid tumors.
文摘当前基于三维点云的工业异常检测仍处于起步阶段,较先进的方法将点云栅格化成2D图片或采用传统算子提取点云特征,有待进一步研究。对此,提出一种基于3D归一化流的双分支点云异常检测模型。以3D归一化流作为整体框架,结合双分支交互融合模块进行局部特征聚合,以并行结构聚合由Transformer和卷积神经网络(Convs)架构提取的不同焦点的点云特征;通过3D归一化流对特征金字塔在欧氏空间和潜在空间之间的可逆映射进行建模,基于受试者曲线下面积,所提模型从分布学习的角度实现了图像级(I-AUROC)的高效异常检测。在MVTec-3D AD数据集上进行的实验结果表明,所提模型在点云异常检测中取得了比目前先进方法更好的性能,相较于M3DM(Multi-3D-Memory)、AST(Asymmetric Student-Teacher Networks),I-AUROC(Image-level Area Under the Receiver Operating Characteristiccurve)指标分别提升了1.8和5.9个百分点。
基金Project supported by the National Natural Science Foundation of China(Grant No.51107016)the National Basic Research Program of China(Grant No.2013CB035605)the Postdoctoral Science Research Developmental Foundation of Heilongjiang Province,China(Grant No.LHB-Q12086)
文摘Complex dynamical phenomenon was studied in the single phase H-bridge inverter which was controlled by either a peak current or a valley current. The state functions and the discrete iterative map equations were established to analyze the dynamical phenomenon in the single phase H-bridge inverter. The dynamical characteristics of the single phase H- bridge inverter, such as time domain waveform diagram, bifurcation diagram, and folding map, were obtained by using the numerical calculation when the circuit parameters varied in specific range. Moreover, the simulation results were obtained by using the OrCAD-PSpice software to validate the numerical calculation. Both the numerical calculation and the circuit simulation show that the symmetrical dynamical phenomenon occurs in the single phase H-bridge inverter controlled by the peak current or the valley current.
