An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The...An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H2O2 formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10-5 to 2 × 10-4 M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.展开更多
Anion-selective electrodes based on dissociated ion-exchangers such as lipophilicquaternary ammonium or phosphonium species always display classical Hofmeister be-havior in the following order: ClO<sub>4</sub...Anion-selective electrodes based on dissociated ion-exchangers such as lipophilicquaternary ammonium or phosphonium species always display classical Hofmeister be-havior in the following order: ClO<sub>4</sub><sup>-</sup>】SCN<sup>-</sup>】I<sup>-</sup>】Br<sup>-</sup>】NO<sub>2</sub><sup>-</sup>】Cl<sup>-</sup>】SO<sub>4</sub><sup>2-</sup>.A new sol-vent polymeric membrane electrode based on Schiff base complexes of Co（Ⅱ）[Co（Ⅱ）S]and showing excellent selectivity toward iodide ion is for the first time prepared inour work. The resulting electrodes exhibit fairly low detection limits andpotentiometric anion-selectivity sequences deviated from the Hofmeister pattern.Bis（salicylaldehyde） ethylenediiminecobalt（Ⅱ） [Co（Ⅱ）（salen）], bis（salicylaldehyde）-phenyldiiminecobalt（Ⅱ） [Co（Ⅱ）（salophen）],展开更多
基金the Gazi University research fund for the financial support of this research.
文摘An electrode for uric acid has been prepared by using an iodide selective electrode with the uricase enzyme. The iodide selective electrode used was prepared from 10% TDMAI and PVC according to our previous study. The enzyme was immobilized on the iodide electrode by holding it at pH 7 phosphate buffer for 20 min at room temperature. The H2O2 formed from the reaction of uric acid was determined from the decrease of iodide concentration that was present in the reaction cell. The potential change was linear in the 2 × 10-5 to 2 × 10-4 M uric acid concentration (3 - 34 mg uric acid/100ml blood) range. Uric acid contents of some blood samples were determined with the new electrode and consistency was obtained with a colorimetric method. The effects of pH, iodide concentration, the amount of enzyme immobilized and the operating temperature were studied. No interference of ascorbic acid, glucose and urea was observed.
基金National Natural Science Foundation of ChinaElectroanalytical Chemistry Laboratory, Changchun Institute of Applied Chemistry, Academia Sinica.
文摘Anion-selective electrodes based on dissociated ion-exchangers such as lipophilicquaternary ammonium or phosphonium species always display classical Hofmeister be-havior in the following order: ClO<sub>4</sub><sup>-</sup>】SCN<sup>-</sup>】I<sup>-</sup>】Br<sup>-</sup>】NO<sub>2</sub><sup>-</sup>】Cl<sup>-</sup>】SO<sub>4</sub><sup>2-</sup>.A new sol-vent polymeric membrane electrode based on Schiff base complexes of Co（Ⅱ）[Co（Ⅱ）S]and showing excellent selectivity toward iodide ion is for the first time prepared inour work. The resulting electrodes exhibit fairly low detection limits andpotentiometric anion-selectivity sequences deviated from the Hofmeister pattern.Bis（salicylaldehyde） ethylenediiminecobalt（Ⅱ） [Co（Ⅱ）（salen）], bis（salicylaldehyde）-phenyldiiminecobalt（Ⅱ） [Co（Ⅱ）（salophen）],
