Curative effect of hydrogen peroxide combined with silver ion disinfection on pelvic floor dysfunction

BACKGROUND Pelvic floor dysfunction(PFD)is related to muscle fiber tearing during childbirth,negatively impacting postpartum quality of life of parturient.Appropriate and effective intervention is necessary to promote PFD recovery.AIM To analyze the use of hydrogen peroxide and silver ion disinfection for vaginal electrodes in conjunction with comprehensive rehabilitation therapy for postpartum women with PFD.METHODS A total of 59 women with PFD who were admitted to the hospital from May 2019 to July 2022 were divided into two groups:Control group(n=27)received comprehensive rehabilitation therapy and observation group(n=32)received intervention with pelvic floor biostimulation feedback instrument in addition to comprehensive rehabilitation therapy.The vaginal electrodes were disinfected with hydrogen peroxide and silver ion before treatment.Intervention for both groups was started 6 weeks postpartum,and rehabilitation lasted for 3 months.Pelvic floor muscle voltage,pelvic floor muscle strength,vaginal muscle voltage,vaginal muscle tone,pelvic floor function,quality of life,and incidence of postpartum PFD were compared between the two groups.RESULTS Before comprehensive rehabilitation treatment,basic data and pelvic floor function were not significantly different between the two groups.After treatment,the observation group showed significant improvements in the maximum voltage and average voltage of pelvic floor muscles,contraction time of type I and type II fibers,pelvic floor muscle strength,vaginal muscle tone,vaginal muscle voltage,and quality of life(GQOLI-74 reports),compared with the control group.The observation group had lower scores on the pelvic floor distress inventory(PFDI-20)and a lower incidence of postpartum PFD,indicating the effectiveness of the pelvic floor biostimulation feedback instrument in promoting the recovery of maternal pelvic floor function.CONCLUSION The combination of the pelvic floor biostimulation feedback instrument and comprehensive rehabilitation nursing can improve pelvic floor muscle strength,promote the recovery of vaginal muscle tone,and improve pelvic floor function and quality of life.The use of hydrogen peroxide and silver ion disinfectant demonstrated favorable antibacterial efficacy and is worthy of clinical application.

THE EFFECT OF TRANSITION METAL IONS-IRON ON HYDROGEN PEROXIDE BLEACHING

Hydrogen peroxide bleaching has been extensivelyused in high-yield pulp bleaching. Unfortunately,hydrogen peroxide can be decomposed underalkaline condition, especially when transition metalions exit. Experiments show that the valence oftransition metal ion is also responsible for thedecomposition of hydrogen peroxide.Iron ions are present in two oxidation states, Fe2+ andFe3+. They are both catalytically active to hydrogenperoxide decomposition. Because Fe3+ is brown, itcan affect the brightness of pulp directly, it can alsocombine with phenol, forming complexes which notonly are stable structures and are difficult to beremoved from pulp, but also significantly affect thebrightness of pulp because of their color.Sodium silicate and magnesium sulfate, when usedtogether, can greatly decrease hydrogen peroxidedecomposition. The optimum dosage of sodiumsilicate is about 0.1% (on solution) for Fe2~ and0.25% (on solution) for Fe3~. Adding chelants such asDTPA or EDTA with stabilizers simultaneously canobviously improve pulp brightness. For iron ions, thechelate effect of DTPA is better than that of EDTA.Under acidic conditions, sodium hyposulfite andcellulose can reduce Fe3+ to Fez+ effectively, and pulpbrightness is improved greatly. Adding sodiumthiosulfate simultaneously with magnesium sulfate,sodium silicate, and DTPA to alkaline peroxidesolution can result in higher brightness of pulp.pH is a key parameter during hydrogen peroxidebleaching, the optimum pH value should be 10.5-12.

THE EFFECT OF TRANSITION METAL IONS-MANGANESE ON HYDROGEN PEROXIDE BLEACHING

In this investigation, the catalytic activities of Mn(II),Mn(III) and Mn(IV) towards decomposing hydrogenperoxide were compared. Among Mn (II), Mn (III)and Mn (IV), Mn (II) is not catalytically active indecomposing hydrogen peroxide. However, both Mn(113) and Mn (IV) are, and Mn (III) has a strongereffect than Mn(IV).In addition, we also studied the practical methods todecrease the Mn-induced decomposition of hydrogenperoxide. The results showed that sodium silicate andmagnesium sulfite in combination can effectivelydecrease the decomposition of hydrogen peroxide.The optimum dosage of sodium silicate was about0.5% (on solution). Adding chelants such as DTPAor EDTA simultaneously with stabilizers candecrease hydrogen peroxide decomposition. For Mn(IV), the EDTA is more effective than DTPA.Adding sodium thiosulfate simultaneously withmagnesium sulfate, sodium silicate and DTPA toalkaline peroxide solution can result in more residualhydrogen peroxide, and a higher pulp brightness.

Determination of Chlorine and Bromine in Plant Materials by Ion Chromatography

A method was developed for determination of chlorine and bromine in plant materials by ion chromatography using temperature programing-semi molten for sample preparation. Values of detection limits of the method found were 1.0×10^-5 for CI and 1.3×10^-6 for Br. The measuring range of the method found were 0.3-20.0 mg/L for CI and 4,0-120,0 μg/L for Br. The results obtained agreed quite well with those reference values.

Chromium Differentially Affects Hydrogen Peroxide Distribution in Primary and Adventitious Roots of Arabidopsis thaliana L.

The post-embryonic growth of the Arabidopsis thaliana root system can be modified by different types of stress,such as sublethal concentrations of metals,which may induce the production of reactive oxygen species(ROS).In this study,the effects of different concentrations of potassium chromate(K_(2)CrO_(4))on the distribution and relative quantity of hydrogen peroxide(H_(2)O_(2))were determined in primary and adventitious roots in A.thaliana HyPer line seedlings.This line has a biosensor that specifically reports H_(2)O_(2) levels within tissues as fluorescence.Primary root growth was inhibited at 100μM Cr(VI);in contrast,adventitious root formation was induced over the main root growth axis.These structures proliferated from 100-160μM Cr(VI),and much higher concentrations(180-200μM)of K_(2)CrO_(4) were required to affect their growth.The H_(2)O_(2) distributions were observed in the columella and lateral root cap of primary roots of plants grown in medium lacking dichromate,but following the development of toxicity symptoms,H_(2)O_(2) changed its distribution to the meristem and differentiation zones.Conversely,adventitious roots had comparable H_(2)O_(2) distribution patterns in untreated plants and those exposed to Cr(VI)supplementation.Thus,differential H_(2)O_(2) distribution correlates with the resistance of adventitious roots,but not primary roots,to dichromate and underlies cell reorganization at the apex to support growth.

Detection of Hydrogen Peroxide Photogenerating from a Hypocrellin B Derivative

In order to f urther improve the photosensitizing activity of hypocrellin B(HB), the complex o f 5,8 di Br HB with Al 3+ was designed and synthesized in high yield. Th e complex of aluminium ion with 5,8 di Br HB is a new water soluble perylene quinonoid derivative with enhanced absorption over HB in the phototherapeutic wi ndow (600-900 nm). Electron paramagnetic resonance (EPR) measurement and 9,10 diphenyl anthracene bleaching methods were used to investigate the photosensiti zing activity of [Al 2(5,8 di Br HB)Cl 4] n in the prese nce of oxygen. Singlet oxygen, superoxide anion radical, hydroxyl radical can be generated by [Al 2(5,8 di Br HB)Cl 4] n photosensit ization. The results showed that the production of hydroxyl radical ( · OH) by [Al 2(5,8 di Br HB)Cl 4] n photosensitization comes from the Fenton Haber Weiss reaction and the decom position of DMPO 1O 2 adduct. Formation of H 2O 2 as one of main intermedi ates in the photogeneration of hydroxyl radical was detected by using the cataly zed oxidation of the DPD reagent by the POD enzyme method. Moreover, the experim ents of EPR spin trap and catalase enzyme excluded the effect of organoperoxide on DPD oxidization. These results further support the proposed mechanism of · OH formation.

Impact of Hydrogen Ion Concentration on Amino Acids Composition of Macadamia Protein: Approached Using Cation-Exchange Chromatography

In the present context, the objective of this study was to synthesize and analyze the content of AA of macadamia protein and the impact of hydrogen ion concentration (pH) on AA composition. The determination of AA mainly by cation-exchange chromatography was also investigated. Reproducible and reliable techniques for quantification and identification of AA usually require derivatization. However, techniques such as AA analyzer are composed of cation-exchange chromatography and other components can sideline the derivatization with significant accuracy. The present analysis revealed a higher concentration of essential amino acids especially acidic AA, Glu and Asp and basic AA, Arg than other AA in macadamia protein. The study constitutes first report of use of bubble chart for evaluation of AA and explaination of AAS. The results may elaborate that the degradation of AA of macadamia protein for extraction of pH 11 is caused by the impact of pH. Moreover, the nutritional values of AA present in macadamia protein could change for the better by adjusting pH of extraction.

Co-intensification of cyanide leaching gold by mercury ions and oxidant

The effects of mercury ions on gold cyanidation were studied. The results show that under low cyanide concentration, gold cyanide process is controlled by CN- transfer, while at higher cyanide concentration, there forms passivation on gold surface. Therefore, chemical oxidation of gold in cyanide solution of higher concentration is controlled by surface reaction. Small quantity of additions of mercury ions bring about great increases in anodic gold dissolution rate, decreases the passivation and reduces the equilibrium activated energy. In addition, they also markedly change the effect pattern of cyanide concentration. Mercury ions show positive effects on cathodic reduction of oxygen and raise the rate of electrochemical step of the cathodic reduction of oxygen. Addition of a certain amount of hydrogen peroxide is confirmed to be an effective way for intensification of cathodic process on gold electrode. Active potential range and current peak on anodic dissolution are enlarged when being co-intensified with Hg^2＋ and hydrogen peroxide. Co-intensifying effect may be obtained and gold from gold concentrates. gold leaching rate is considerably increased on cyanide leaching of

Determination of haloacetic acids in hospital effuent after chlorination by ion chromatography

The ion chromatography combined solid phase extraction （SPE） method was developed for the analysis of low concentration haloacetic acids （HAAs）, a class of disinfection by-products formed from chlorination of hospital wastewater. The monitored HAAs included monochloroacetic acid, monobromoacetic acid, dichloroacetic acid, dibromoacetic acid and trichloroacetic acid. The method employed a sodium hydroxide eluent at a flow rate of 0.8 ml/min, electrolytically generated gradients, and suppressed conductivity detection. To analyze the HAAs in real hospital wastewater samples, C18 pretreatment cartridge was utilized to reduce samples＇ turbidity. Preconcentration with SPE and matrix elimination with treatment cartridges were investigated and found to be able to obtain acceptable detection limits. Linearity, repeatability and detection limits of the above method were evaluated. The detection limits of monobromoacetic acid and dibromoacetic acid were 2.61 μg/L and 1.30 μg/L, respectively, and the other three acids are ranging from 0.48 to 0.82μg/L under 25-fold preconcentration. When the above optimization procedure was applied to three hospital wastewater samples with different treatment processes in Tianjin, it was found that the dichloroacetic acid was the major compound, and the growth ratios of the HAAs after disinfection by sodium hypochlorite were 91.28%, 63.61% and 79.50%, respectively.

Involvement of Acid-sensing Ion Channel 1a in Functions of Cultured Human Retinal Pigment Epithelial Cells

In the retina, pH fluctuations may play an important role in adapting retinal responses to different light intensities and are involved in the fine tuning of visual perception. Acidosis occurs in the subretinal space （SRS） under pathological conditions such as age-related macular degeneration （AMD）. Although it is well known that many transporters in the retinal pigment epithelium （RPE） cells can maintain pH homeostasis efficiently, other receptors in RPE may also be involved in sensing acidosis, such as acid-sensing ion channels （ASICs）. In this study, we investigated whether ASICla was ex- pressed in the RPE cells and whether it was involved in the function of these cells. Real-time RT-PCR and Western blotting were used to analyze the ASICla expression in ARPE-19 cells during oxidative stress induced by hydrogen peroxide （H202）. Furthermore, inhibition or over-expression of ASICla in RPE cells was obtained using inhibitors （amiloride and PCTxl） or by the transfection of cDNA encod- ing hASICla. Cell viability was determined by using the MTT assay. The real-time RT-PCR and West- ern blotting results showed that both the mRNA and protein of ASICla were expressed in RPE cells. In- hibition of ASICs by amiloride in normal RPE cells resulted in cell death, indicating that ASICs play an important physiological role in RPE cells. Furthermore, over-expression of ASICla in RPE cells pro- longed cell survival under oxidative stress induced by H2O2. In conclusion, ASICla is functionally expressed in RPE cells and may play an important role in the physiological function of RPE cells by pro-tecting them from oxidative stress.

Luminal oxidants selectively modulate electrogenic ion transport in rat colon

AIM： To investigate the effects of luminal exposure to H2O2 and two related thiol oxidizing agents on basal and stimulated chloride secretion in native colon using electrophysiological and pharmacological approaches. METHODS： Unstripped rat distal colon segments were mounted in Ussing chambers. Potential difference, calculated resistance and short-circuit current across unstripped colon segments were monitored with a dual voltage/current clamp. Paracellular permeability was assessed by measuring the mucosa-to-serosa flux of a fluorescent probe （FITC）. RESULTS： Luminal exposure to hydrogen peroxide transitorily stimulated chloride secretion without altering barrier function. This stimulatory effect could be blocked by basolateral atropine but not indomethacin. The cysteine and methionine oxidizing compounds, phenylarsine oxide and chloramine T respectively, mimicked the effect of H2O2, except for a drop in transcolonic resistance after 30 min. In contrast to the observed stimulatory effect on basal secretion, cAMP-stimulated electrogenic ion transport was blunted by luminal H2O2. However, the Ca^2＋- activated response remained unchanged. CONCLUSION： H2O2 may be an important selective modulator of intestinal ion and water secretion in certain pathologic conditions such as inflammation or ischemiareperfusion by multiple mechanisms.

Corrosion Behaviors of Disinfectants on Dental Unit Waterlines

To investigate the corrosion behaviors and antibacterial effects of sodium hypochlorite(NaClO)and hydrogen peroxide silver ion(HPSI)disinfectants with different concentrations against dental unit waterlines and provide guidance and reference for the use of chemical disinfectants,polyurethane tubes were immersed in ultrapure water(control group),0.1%NaClO,0.5%NaClO,1.0%NaClO,2.5%HPSI,5.0%HPSI,and 10%HPSI solutions for 6,12,and 18 weeks.Contact angles and Fourier transform infrared spectra were detected.Surface morphologies were observed using scanning electron microscopy and antibacterial activity was evaluated using Gram-positive Staphylococcus aureus(S.aureus).The results showed that sodium hypochlorite and hydrogen peroxide silver ion disinfectants presented good antibacterial activity against S.aureus.However,sodium hypochlorite could cause serious damage to the water pipes where corrosion pits and cracks were observed,and increasing the concentration of sodium hypochlorite could accelerate the corrosion process.Hydrogen peroxide silver ion disinfectants had no obvious damage to the water pipes.Therefore,hydrogen peroxide silver ion disinfectants are recommended to use for controlling bacterial infection in dental unit waterlines which can reduce the damage to the water pipes.

在线燃烧离子色谱法测定环氧树脂中的氯和溴

建立了在线燃烧离子色谱同时测定环氧树脂中氯和溴的分析方法.采用高温裂解炉与离子色谱的在线联用装置,通过对样品进行高温燃烧裂解和气化,以100 mg·L^(-1)过氧化氢为吸收液,将产生的卤化氢气体吸收并转化为无机阴离子,离子色谱法进行样品的测定,以氯和溴的峰面积外标法进行定量.对影响燃烧效果和测定结果准确度的因素如燃烧时间、燃烧温度、吸收液体积、称样量、氧气和氩气流速等条件进行了选择优化.在(0.10—2.50)mg·L^(-1)和(0.02—0.50)mg·L^(-1)范围内,氯和溴离子的线性相关系数(r^(2))大于0.999,该方法对于氯和溴的定量下限分别为0.55 mg·kg^(-1)和2.60 mg·kg^(-1).采用建立的方法分别对环氧树脂和EC680k低密度聚乙烯标准物质中的氯和溴进行测定,并与传统的氧弹燃烧-离子色谱法进行了比较.结果表明:在线燃烧离子色谱法对于环氧树脂中氯和溴测定结果的相对标准偏差分别为1.28%和2.29%,测定值与氧弹燃烧-离子色谱法基本一致,EC680k的测定结果与标准值符合,证明该方法具有良好的准确度和精密度.该方法准确度和灵敏度高、重复性较好,能够满足批量树脂类样品中氯和溴的含量筛查和多批次产品的质量控制.

离子色谱法测定酒石酸沃尼妙林中酒石酸的含量

建立离子色谱-抑制电导检测法测定化学对照品中酒石酸盐的含量。以酒石酸沃尼妙林对照品为例,采用Dionex IonPac^(TM) AS19(4×250 mm)阴离子交换色谱柱和Dionex IonPac^(TM) AG18(4×50 mm)保护柱,以22 mmol/L氢氧化钾溶液为流动相,流速为1.0 mL/min等度洗脱,抑制电导检测器检测,测定酒石酸盐中酒石酸的含量。结果表明,该方法在1.0~50μg/mL浓度范围内线性关系良好(r=0.999),定量限为0.5μg/mL,酒石酸沃尼妙林在3个水平的加样回收率分别为98.79%、98.91%与96.50%,平均回收率为98.07%(RSD=2.20%,n=9)。用此方法测定3个厂家共5批样品,酒石酸沃尼妙林中酒石酸含量的数值与理论值基本一致。本文建立的离子色谱法准确、灵敏,可用于酒石酸盐成盐比例与含量的测定。

过氧化氢预处理-硝酸银滴定法测定氯离子的研究

氯离子(Cl^(-))测定在研究氯营养和盐胁迫过程中起着关键作用,但是样品的预处理仍然是检测Cl^(-)的一个难题,针对植物、土壤、肥料、水体并没有统一的样品预处理方法。基于硝酸银(AgNO_(3))滴定法测定Cl^(-)的机理,研究过氧化氢(H_(2)O_(2))预处理样品测定Cl^(-)的原理和方法。结果表明,H_(2)O_(2)在非浓盐酸环境下不能氧化Cl^(-)而生成氯气(Cl_(2));H_(2)O_(2)电离出的氢离子(H^(+))会与Cl^(-)结合生成氯化氢(HCl),在加热条件下挥发导致Cl^(-)损失;H_(2)O_(2)和试样在加热回流冷凝条件下可对样品进行脱色和有机物降解,既不会造成Cl^(-)损失,又利于后续滴定终点判断;H_(2)O_(2)预处理方法适用于植物、土壤和浑浊水样中Cl^(-)的测定;该方法具有操作简单、无二次污染、测定结果准确度和精密度高等优点。为建立不同基质样品中Cl^(-)检测的统一标准测试方法奠定了理论和实践基础。

三种不同消毒剂对口腔综合治疗台水路消毒效果的比较

目的比较3种不同消毒剂对口腔综合治疗台水路(DUWLs)的消毒效果。方法选取本院2019年装修后待开诊前7台采用独立水源的综合治疗台,分为4组(3组实验组+1组对照组),实验组分别采用500 mg/L含氯消毒剂(A组2台综合治疗椅)、3%过氧化氢(B组2台综合治疗椅)、60 g/L过氧化氢银离子消毒剂(C组2台综合治疗椅)和净化后市政用水纯水(D组对照组1台综合治疗椅),通过3种不同的消毒剂对DUWLs进行消毒,然后收集各组三用枪和高速手机水样进行细菌培养,比较各组DUWLs消毒前及消毒后即刻、1d、3d、7d细菌菌落总数。结果消毒前DU⁃WLs各组细菌菌落总数均严重超标(均>4500 cfu/mL)。3种消毒剂消毒后即刻和消毒后1 d细菌菌落总数明显下降,低于消毒前及对照组。消毒后3 d,除实验C组外,实验A、B两组细菌菌落总数均超过北京市《口腔综合治疗台水路消毒技术规范(DB11/T 1703-2019)》规定的口腔医疗卫生用水标准。消毒后7 d,A、B、C三组细菌菌落总数均接近或超过消毒前水平。结论500 mg/L含氯消毒剂和3%过氧化氢消毒剂对水路消毒效果维持时间短,长期使用对管路内壁造成不可修复的危害。60g/L过氧化氢银离子消毒剂在控制口腔牙椅水路(DU⁃WLs)细菌落数总数和抑制细菌生长时间方面效果最优。

离子色谱法和离子选择电极法对氟化氢溶液中的氟化物分析对比

氟化物指含负价氟的有机或无机化合物。与其他卤素类似,氟生成单负阴离子。无机氟化物的例子有氢氟酸(HF)、氟化钠(NaF)等。本文用离子选择电极法和离子色谱法分别分析含氢氟酸(HF)溶液中的氟化物。发现溶液含有氢氟酸(HF)时分析氟化物,分析结果与p H值形成干扰,pH值越低氟化物的结果越高,而采用氢氧化钠将溶液中和成碱性条件和用碳酸钠-碳酸氢钠淋洗液体系稀释样品时,干扰最小。而含氢氟酸(HF)溶液采用离子选择电极法分析时溶液pH值偏低,H+偏高,会氟化物的浓度偏高,而溶液p H值调至中性条件时干扰最小,结果最接近真值。

氢氧化钠吸收液-离子色谱法测固定污染源废气中的氯气

建立了氢氧化钠吸收液-离子色谱测定固定污染源废气中氯气的方法。研究采样和分析条件,采用50 mL冲击式吸收瓶,以氢氧化钠为吸收液,利用硫代硫酸钠将样品中的次氯酸完全转化为氯离子,过滤后用离子色谱进行分析。可在采样时选择0.3μm石英滤膜消除颗粒物干扰,在氢氧化钠吸收液前串联硫酸吸收液消除氯化氢干扰。测试结果显示:氯离子曲线线性关系良好,相关系数为0.9990;方法检出限为0.02 mg/m^(3),测定下限为0.08 mg/m^(3);空白加标的相对标准偏差为0.82%~1.4%,加标回收率为98.0%~107%;实际样品基体加标的相对标准偏差为1.8%~2.7%,加标回收率为98.0%~104%。测试结果表明该方法具有较高的精密度和正确度。

全自动高温水解-离子色谱法测定氧化镧铈中氟、氯

为探究稀土氧化物中氟(F)和氯(Cl)元素含量的快速检测方法,通过全自动高温水解仪对氧化镧铈样品进行前处理,并利用氢氧根体系离子色谱仪检测吸收液中F^(-)、Cl^(-)的含量,建立了基于全自动高温水解-离子色谱法测定氧化镧铈中的F、Cl元素含量的快速检测方法,有效避免了传统前处理方法过程复杂、分析时间长、极易受人为干扰的问题。将氧化镧铈样品,在氧气流量为300 mL/min,1100℃高温燃烧水解20 min,以NaOH(15 mmol/L)作为淋洗液,经色谱柱分离,测得F^(-)与Cl^(-)在质量浓度为1.00~15.00 mg/L时,质量浓度与离子色谱峰面积呈线性关系,相关系数均为0.9999,检出限分别为0.003 mg/L和0.12 mg/L。全自动高温水解仪联用离子色谱仪检测系统对氧化镧铈中F^(-)的平均加标回收率为98.4%,标准偏差RSD为0.94%;对Cl^(-)的加标回收率为97.8%,RSD为2.9%。方法具有较高准确度及精密度,测试结果准确、可靠,满足测试要求,为稀土氧化镧铈中氟、氯元素含量的研究及相关产品的开发提供了理论基础。

解钼液双氧水氧化除S^(2-)试验研究

采用双氧水氧化-硫酸调酸的方式处理吸附硫代钼酸根饱和树脂的解吸液(解钼液),研究了氧化剂种类、用量和添加方式以及反应温度和时间对解钼液中S^(2-)去除效果的影响。结果表明:在反应温度35℃、搅拌速度恒定(200 r/min)、双氧水用量为理论量的1.0倍并一次性添加以及反应时间20 min条件下,解钼液中S^(2-)的浓度可降到0.01 g/L以下,调酸过程未检测到硫化氢气体和三硫化钼渣。此方法为离子交换工段解钼液的绿色、高效处理提供了一种参考。