he present paper covers a model involving Donnan memhrane equilibrium andadsorption eluilibrium to describe the retention process for diprotic organic acids inion-exclusion chromatography. On this basis the microcompu...he present paper covers a model involving Donnan memhrane equilibrium andadsorption eluilibrium to describe the retention process for diprotic organic acids inion-exclusion chromatography. On this basis the microcomputer simulation of elu-tion behaviour for dicarboxylic acids was investigated. Influences of eluent acidityand sample concentration on the retention value were studied. The theoreticallypredicted results are in reasonable agreement with the experimental data.展开更多
A simple, rapid and accurate ion-exclusion chromatographic method coupled with a UV detector for the determination of uric acid in human urine samples has been developed. The separation was carried out on an ion-exclu...A simple, rapid and accurate ion-exclusion chromatographic method coupled with a UV detector for the determination of uric acid in human urine samples has been developed. The separation was carried out on an ion-exclusion column using only pure water as mobile phase. The detection wavelength was 254 nm and urine sample was injected directly without any pretreatment. Furthermore, the retention behavior of uric acid on the ion-exclusion column was researched when pure water and 1 mmol·L-1 HCI were used as mobile phase, respectively. The stability of uric acid was also further investigated within 28 days, In this method, the linear range of the calibration curve for uric acid was 0.25--100 mg·L-1, and the detection limit calculated at S/N=3 was 0.02mg·L-1 The proposed ion-exclusion chromatographic method has been used for the determination of uric acid in human urine.展开更多
建立了离子排斥色谱法（ ion-exclusion chromatography，IEC）测定黄酒中有机酸含量的分析方法。使用Waters 离子排斥色谱柱（300 mm×7.8 mm，7μm），流动相为 H 2 SO 4溶液（ A）与乙腈（ B）的混合溶液（体积比为98：2），线...建立了离子排斥色谱法（ ion-exclusion chromatography，IEC）测定黄酒中有机酸含量的分析方法。使用Waters 离子排斥色谱柱（300 mm×7.8 mm，7μm），流动相为 H 2 SO 4溶液（ A）与乙腈（ B）的混合溶液（体积比为98：2），线性梯度程序：0～40 min，流动相 A 的浓度由0.01 mol / L 上升到0.02 mol / L；40～50 min，流动相 A 的浓度为0.01 mol / L ；流速为0.5 mL / min，柱温50℃，进样量10μL，检测波长210 nm。结果表明，该方法可在30 min内实现草酸、马来酸、柠檬酸、酒石酸、苹果酸、抗坏血酸、琥珀酸、乳酸、富马酸、乙酸、丙酸、异丁酸和丁酸的完全分离与定量，13种有机酸在0.001～1.000 g / L 范围内线性关系良好，回归方程的线性相关系数在0.9997以上。黄酒中13种有机酸的加标回收率为93.4％～103.8％，相对标准偏差为0.1％～1.5％（n =5）。该方法简单快捷、准确、重复性好，可用于黄酒中有机酸的测定。展开更多
By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by th...By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable, and can be scaled up easily.展开更多
A unified ion-exclusion chromatography(IEC) system for monitoring anionic and cationic nutrients like NH + 4,NO 2,NO 3,phosphate ion,silicate ion and HCO 3 was developed and applied to several environmental waters.The...A unified ion-exclusion chromatography(IEC) system for monitoring anionic and cationic nutrients like NH + 4,NO 2,NO 3,phosphate ion,silicate ion and HCO 3 was developed and applied to several environmental waters.The IEC system consisted of four IEC methodologies,including the IEC with ultraviolet(UV) detection at 210 nm for determining NH + 4 on anion-exchange separation column in OH form connected with anion-exchange UV-conversion column in I form in tandem,the IEC with UV-detection at 210 nm for determining simultaneously NO 2 and NO 3 on cation-exchange separation column in H + form,the IEC with UV-detection at 210 nm for determining HCO 3 on cation-exchange separation column in H + form connected with anion-exchange UV-conversion column in I form in tandem,and the IEC with visible-detection based on molybdenum-blue reaction for determining simultaneously silicate and phosphate ions on cation-exchange separation column in H + form.These IEC systems were combined through three manually-driven 6-port column selection valves to select each separation column to determine selectively the ionic nutrients.Using this sequential water quality monitoring system,the analytical performances such as calibration linearity,reproducibility,detection limit and recovery were also tested under the optimized chromatographic conditions.This novel water quality monitoring system has been applied successfully for the determination of the ionic eutrophication components in sub-urban river waters.展开更多
在制备6-磷酸甘露糖过程中,将6-磷酸甘露糖与磷酸根杂质分开是纯化过程和建立质量标准的重要环节。本文建立了6-磷酸甘露糖和磷酸根的离子色谱分离-电化学检测方法。样品经溶解、过滤后进行色谱分析,以Ion-Pac AG18离子交换柱(50 m m...在制备6-磷酸甘露糖过程中,将6-磷酸甘露糖与磷酸根杂质分开是纯化过程和建立质量标准的重要环节。本文建立了6-磷酸甘露糖和磷酸根的离子色谱分离-电化学检测方法。样品经溶解、过滤后进行色谱分析,以Ion-Pac AG18离子交换柱(50 m m×4 m m)为保护柱,在Io npac AS18离子交换色谱柱(250 m m×4 m m)上分离,以25 m m o l/L氢氧化钾溶液为流动相,等度淋洗,流速1.0 m L/m in,安培检测器和电导检测器串联检测,外标法定量。先使用安培检测器检测,在碱性条件下,6-磷酸甘露糖在安培检测器上被选择性检出;后使用电导检测器检测,经ASRS型抑制器抑制背景电导后,6-磷酸甘露糖和磷酸根同时被电导检测器检出,二者分离度良好。采用安培检测器检测时,进样量为25μL,6-磷酸甘露糖的线性范围为0.06～10.00 mg/L,相关系数为0.999 8,回收率为92.1%～103.1%,相对标准偏差均小于3%,检出限(以信噪比为3计)为0.02 m g/L。该方法灵敏度高,无杂质干扰,前处理简便,可用于原料药合成中间体的检测,结果令人满意。展开更多
文摘he present paper covers a model involving Donnan memhrane equilibrium andadsorption eluilibrium to describe the retention process for diprotic organic acids inion-exclusion chromatography. On this basis the microcomputer simulation of elu-tion behaviour for dicarboxylic acids was investigated. Influences of eluent acidityand sample concentration on the retention value were studied. The theoreticallypredicted results are in reasonable agreement with the experimental data.
文摘A simple, rapid and accurate ion-exclusion chromatographic method coupled with a UV detector for the determination of uric acid in human urine samples has been developed. The separation was carried out on an ion-exclusion column using only pure water as mobile phase. The detection wavelength was 254 nm and urine sample was injected directly without any pretreatment. Furthermore, the retention behavior of uric acid on the ion-exclusion column was researched when pure water and 1 mmol·L-1 HCI were used as mobile phase, respectively. The stability of uric acid was also further investigated within 28 days, In this method, the linear range of the calibration curve for uric acid was 0.25--100 mg·L-1, and the detection limit calculated at S/N=3 was 0.02mg·L-1 The proposed ion-exclusion chromatographic method has been used for the determination of uric acid in human urine.
文摘建立了离子排斥色谱法（ ion-exclusion chromatography，IEC）测定黄酒中有机酸含量的分析方法。使用Waters 离子排斥色谱柱（300 mm×7.8 mm，7μm），流动相为 H 2 SO 4溶液（ A）与乙腈（ B）的混合溶液（体积比为98：2），线性梯度程序：0～40 min，流动相 A 的浓度由0.01 mol / L 上升到0.02 mol / L；40～50 min，流动相 A 的浓度为0.01 mol / L ；流速为0.5 mL / min，柱温50℃，进样量10μL，检测波长210 nm。结果表明，该方法可在30 min内实现草酸、马来酸、柠檬酸、酒石酸、苹果酸、抗坏血酸、琥珀酸、乳酸、富马酸、乙酸、丙酸、异丁酸和丁酸的完全分离与定量，13种有机酸在0.001～1.000 g / L 范围内线性关系良好，回归方程的线性相关系数在0.9997以上。黄酒中13种有机酸的加标回收率为93.4％～103.8％，相对标准偏差为0.1％～1.5％（n =5）。该方法简单快捷、准确、重复性好，可用于黄酒中有机酸的测定。
文摘By using ion-exchange preparative chromatography (IEPC) and reversed-phase high performance liquid preparative chromatography(RP-HPLPC), thymosin α 1 was isolated and purified from the crude product synthesized by the solid-phase peptide synthesis(SPPS) method. The purity of the final product reached 95% through ion-exchange chromatography on DEAE Sepharose Fast Flow chromatography and Delta-Pak TM C18 purification after optimizing the chromatographic conditions. The capacity of purification process was 50mg/circle. The total yield was 36%. The technology is simple and reliable, and can be scaled up easily.
基金financially supported from TOSOH Corporation and Fellowships Program for Young Scientists of the Japan Society for the Promotion of Science on FY2011
文摘A unified ion-exclusion chromatography(IEC) system for monitoring anionic and cationic nutrients like NH + 4,NO 2,NO 3,phosphate ion,silicate ion and HCO 3 was developed and applied to several environmental waters.The IEC system consisted of four IEC methodologies,including the IEC with ultraviolet(UV) detection at 210 nm for determining NH + 4 on anion-exchange separation column in OH form connected with anion-exchange UV-conversion column in I form in tandem,the IEC with UV-detection at 210 nm for determining simultaneously NO 2 and NO 3 on cation-exchange separation column in H + form,the IEC with UV-detection at 210 nm for determining HCO 3 on cation-exchange separation column in H + form connected with anion-exchange UV-conversion column in I form in tandem,and the IEC with visible-detection based on molybdenum-blue reaction for determining simultaneously silicate and phosphate ions on cation-exchange separation column in H + form.These IEC systems were combined through three manually-driven 6-port column selection valves to select each separation column to determine selectively the ionic nutrients.Using this sequential water quality monitoring system,the analytical performances such as calibration linearity,reproducibility,detection limit and recovery were also tested under the optimized chromatographic conditions.This novel water quality monitoring system has been applied successfully for the determination of the ionic eutrophication components in sub-urban river waters.
文摘在制备6-磷酸甘露糖过程中,将6-磷酸甘露糖与磷酸根杂质分开是纯化过程和建立质量标准的重要环节。本文建立了6-磷酸甘露糖和磷酸根的离子色谱分离-电化学检测方法。样品经溶解、过滤后进行色谱分析,以Ion-Pac AG18离子交换柱(50 m m×4 m m)为保护柱,在Io npac AS18离子交换色谱柱(250 m m×4 m m)上分离,以25 m m o l/L氢氧化钾溶液为流动相,等度淋洗,流速1.0 m L/m in,安培检测器和电导检测器串联检测,外标法定量。先使用安培检测器检测,在碱性条件下,6-磷酸甘露糖在安培检测器上被选择性检出;后使用电导检测器检测,经ASRS型抑制器抑制背景电导后,6-磷酸甘露糖和磷酸根同时被电导检测器检出,二者分离度良好。采用安培检测器检测时,进样量为25μL,6-磷酸甘露糖的线性范围为0.06～10.00 mg/L,相关系数为0.999 8,回收率为92.1%～103.1%,相对标准偏差均小于3%,检出限(以信噪比为3计)为0.02 m g/L。该方法灵敏度高,无杂质干扰,前处理简便,可用于原料药合成中间体的检测,结果令人满意。
