Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The ...Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water.The treated mouse was fed with^(2)H_(2)O(80%,v/v)and the control mouse fed with standard drinking water(H2O)over next 13days.Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1,2,3,10,12,and 13.Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University(approval No.20161117163322)on October 29,2021.Results:We observed a replacement of 1H by^(2)H in 52 mass spectral features(60^(2)H/1H isotopologue pairs)for the mouse fed with^(2)H_(2)O,but not for the control mouse.These included pyruvic acid and lactic acid,lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid,propionic acid,butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo^(2)H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use^(2)H tracing to extend current metabolic studies,especially those with a focus on anaerobic glycolysis,lysine methylation and gut microbiome via monitoring of short-chain fatty acids.展开更多
基金This work was supported by a grant from Fondation Botnar(Switzerland)and the Swiss National Science Foundation(Nos.320030_173168 and PCEGP3_181300,to PS)the National Natural Science Foundation of China(No.22122603,to XL)+1 种基金Guangdong Special Support Program(No.2019BT02Z546,to XL)Guangdong Natural Science Foundation(Nos.2018A030313790 and 2017GC010648,to HZ).
文摘Objective:The aim of this work was to explore the feasibility of in vivo and non-invasive monitoring of deuterium/hydrogen(^(2)H/1H)exchange at the metabolic level upon exposure to heavy water(^(2)H_(2)O).Methods:The healthy female mice were randomly assigned to two groups after day 0 when both mice received standard drinking water.The treated mouse was fed with^(2)H_(2)O(80%,v/v)and the control mouse fed with standard drinking water(H2O)over next 13days.Real-time mass spectrometric analysis of volatile metabolism emitted through breathing and the skin was performed on days 1,2,3,10,12,and 13.Animal experiment was approved by the Laboratory Animal Ethics Committee of Jinan University(approval No.20161117163322)on October 29,2021.Results:We observed a replacement of 1H by^(2)H in 52 mass spectral features(60^(2)H/1H isotopologue pairs)for the mouse fed with^(2)H_(2)O,but not for the control mouse.These included pyruvic acid and lactic acid,lysine and methyl-lysine as well as short-chain fatty acids comprising acetic acid,propionic acid,butyric acid and valeric acid.Conclusion:Secondary electrospray ionization-high resolution mass spectrometry allows monitoring in vivo^(2)H-incorporation of metabolites in a non-invasive and real-time setup and opens new opportunities to use^(2)H tracing to extend current metabolic studies,especially those with a focus on anaerobic glycolysis,lysine methylation and gut microbiome via monitoring of short-chain fatty acids.
