鸦胆子苦醇调节SPHK1/S1P/S1PR3信号通路对卵巢癌细胞恶性生物学行为的影响

目的探讨鸦胆子苦醇调节鞘氨醇激酶1(SPHK1)/鞘氨醇-1-磷酸(S1P)/鞘氨醇-1-磷酸酯受体3(S1PR3)信号通路对卵巢癌细胞恶性生物学行为的影响。方法将体外培养的人卵巢癌细胞株SKOV-3随机分为对照组、鸦胆子苦醇组、SPHK1过表达组、鸦胆子苦醇+空载组、鸦胆子苦醇+SPHK1过表达组,检测各组细胞活力、克隆形成率、迁移数、侵袭数、凋亡率以及增殖相关蛋白[骨髓细胞瘤病毒癌基因同源物(C-myc)]、凋亡相关蛋白[B细胞淋巴瘤2(Bcl-2)、Bcl-2相关X蛋白(Bax)]、上皮间质转化(EMT)相关蛋白[上皮钙黏素(E-cadherin)、神经钙黏素(N-cadherin)]及SPHK1、S1P、S1PR3蛋白表达量。采用SKOV-3细胞构建裸鼠移植瘤模型,随机分为对照组、鸦胆子苦醇低剂量组、鸦胆子苦醇中剂量组、鸦胆子苦醇高剂量组、SPHK1过表达组、鸦胆子苦醇高剂量+空载组、鸦胆子苦醇高剂量+SPHK1过表达组,检测各组移植瘤生长情况;随机分为对照组、鸦胆子苦醇组、SPHK1过表达组、鸦胆子苦醇+空载组、鸦胆子苦醇+SPHK1过表达组,检测各组移植瘤组织中增殖、凋亡、EMT及SPHK1/S1P/S1PR3信号通路相关蛋白表达量。结果体外实验显示,与对照组相比,鸦胆子苦醇组细胞活力、克隆形成率、迁移数、侵袭数和C-myc、Bcl-2、Ncadherin、SPHK1、S1P、S1PR3蛋白表达量均显著降低(P<0.05),凋亡率和Bax、E-cadherin蛋白表达量均显著升高(P<0.05);过表达SPHK1可减弱鸦胆子苦醇对SKOV-3细胞上述指标的影响。体内实验显示,与对照组相比,鸦胆子苦醇低、中、高剂量组裸鼠干预21 d后的移植瘤体积均显著降低并呈剂量依赖性(P<0.05);高剂量鸦胆子苦醇还可显著降低移植瘤组织中C-myc、Bcl-2、Ncadherin、SPHK1、S1P、S1PR3蛋白表达量(P<0.05),显著升高Bax、E-cadherin蛋白表达量(P<0.05);过表达SPHK1可减弱鸦胆子苦醇对移植瘤组织上述指标的影响。结论鸦胆子苦醇可通过下调SPHK1/S1P/S1PR3信号通路蛋白表达,进而抑制卵巢癌细胞体外增殖、克隆、EMT、迁移及侵袭并诱导其凋亡,还可抑制卵巢癌细胞在裸鼠体内的生长,最终抑制其恶性生物学行为,对卵巢癌起到显著的抗癌功效。

Insights on advanced g‐C_(3)N_(4)in energy storage:Applications,challenges,and future

Graphitic carbon nitride(g‐C_(3)N_(4))is a highly recognized two‐dimensional semiconductor material known for its exceptional chemical and physical stability,environmental friendliness,and pollution‐free advantages.These remarkable properties have sparked extensive research in the field of energy storage.This review paper presents the latest advances in the utilization of g‐C_(3)N_(4)in various energy storage technologies,including lithium‐ion batteries,lithium‐sulfur batteries,sodium‐ion batteries,potassium‐ion batteries,and supercapacitors.One of the key strengths of g‐C_(3)N_(4)lies in its simple preparation process along with the ease of optimizing its material structure.It possesses abundant amino and Lewis basic groups,as well as a high density of nitrogen,enabling efficient charge transfer and electrolyte solution penetration.Moreover,the graphite‐like layered structure and the presence of largeπbonds in g‐C_(3)N_(4)contribute to its versatility in preparing multifunctional materials with different dimensions,element and group doping,and conjugated systems.These characteristics open up possibilities for expanding its application in energy storage devices.This article comprehensively reviews the research progress on g‐C_(3)N_(4)in energy storage and highlights its potential for future applications in this field.By exploring the advantages and unique features of g‐C_(3)N_(4),this paper provides valuable insights into harnessing the full potential of this material for energy storage applications.

In situ luminescence measurements of GaN/Al_(2)O_(3) film under different energy proton irradiations

Ion beam-induced luminescence(IBIL) experiments were performed to investigate the in situ luminescence of GaN/Al_(2)O_(3) at varying ion energies,which allowed for the measurement of defects at different depths within the material.The energies of H^(+)were set to 500 keV,640 keV and 2 MeV,the Bragg peaks of which correspond to the GaN film,GaN/Al_(2)O_(3) heterojunction and Al_(2)O_(3) substrate,respectively.A photoluminescence measurement at 250 K was also performed for comparison,during which only near band edge(NBE) and yellow band luminescence in the GaN film were observed.The evolution of the luminescence of the NBE and yellow band in the GaN film was discussed,and both exhibited a decrease with the fluence of H^(+).Additionally,the luminescence of F centers,induced by oxygen vacancies,and Cr^(3+),resulting from the ^(2)E →^(4)A_(2) radiative transition in Al_(2)O_(3),were measured using 2 MeV H^(+).The luminescence intensity of F centers increases gradually with the fluence of H^(+).The luminescence evolution of Cr^(3+)is consistent with a yellow band center,attributed to its weak intensity,and it is situated within the emission band of the yellow band in the GaN film.Our results show that IBIL measurement can effectively detect the luminescence behavior of multilayer films by adjusting the ion energy.Luminescence measurement can be excited by various techniques,but IBIL can satisfy in situ luminescence measurement,and multilayer structural materials of tens of micrometers can be measured through IBIL by adjusting the energy of the inducing ions.The evolution of defects at different layers with ion fluence can be obtained.

血清MPO、PR3和GBM联合检测对自身免疫性血管炎的诊断价值

目的:探讨自身免疫性血管炎诊断中血清抗髓过氧化物酶(myeloperoxidase,MPO)、抗蛋白酶3(protease 3,PR3)和抗肾小球基底膜(glomerular basement membrane,GBM)联合检测的临床价值。方法:选取新乡市第一人民医院2021年1月至2024年3月收治的自身免疫性血管炎患者60例为研究组,另选取同期体检健康人员60例为对照组,所有纳入人员均予以血清MPO、PR3、GBM抗体检测,比较两组MPO、PR3、GBM抗体检验值,以综合诊断结果为金标准,分析MPO、PR3、GBM抗体单项检测及联合检测的诊断效能。结果:较之对照组,研究组MPO、PR3、GBM抗体检验值均明显更高(P<0.05);MPO、PR3、GBM检测自身免疫性血管炎,单项检测与临床综合诊断结果之间的一致性程度分别为中等、中等、一般,Kappa=0.5167、0.4500、0.4000,而MPO联合PR3、MPO联合GBM、PR3联合GBM检测的一致性均较强,Kappa=0.7833、0.7667、0.7500,不过三者联合检测的结果最为接近临床综合诊断结果,一致性程度很强,Kappa=0.9667;较之MPO、PR3、GBM单方法检测或两两联合检测,三者联合检测诊断自身免疫性血管炎的准确度、灵敏度均明显更高(P<0.05);较之MPO单方法检测,MPO、PR3、GBM三者联合检测诊断自身免疫性血管炎的特异度均明显更高(P<0.05)。结论:MPO、PR3、GBM三者联合检测自身免疫性血管炎的诊断准确度、灵敏度及特异度均较高。

槐耳多糖调节SPHK1/S1P/S1PR3信号通路对宫颈癌细胞恶性生物学行为的影响

目的:探究槐耳多糖(HP)调节SPHK1/S1P/S1PR3信号通路对宫颈癌细胞恶性生物学行为的影响。方法:MTT检测槐耳多糖(0、25、50、100、200、400μg/mL)处理的宫颈癌细胞活力,筛选最佳药物浓度。实验分为对照组(Control组)、槐耳多糖低、中、高浓度组(HP-L、HP-M、HP-H组)和槐耳多糖高浓度+SphK1激活剂K6PC-5组(HP-H+K6PC-5组),观察细胞增殖、迁移和侵袭情况;western blot检测SPHK1、S1P、S1PR3、Snail、N-cadherin、E-cadherin蛋白水平。结果:处理24、48、72h后,与0μg/mL比较,50μg/mL、100μg/mL、200μg/mL和400μg/mL的HP处理的细胞活力显著降低(P<0.05)。HP-L组、HP-M组和HP-H组细胞Edu阳性率、侵袭细胞数、划痕愈合率及Snail、N-cadherin、SPHK1、S1P、S1PR3水平显著低于Control组(P<0.05),E-cadherin水平显著升高(P<0.05)。HP-H+K6PC-5组细胞Edu阳性率、侵袭细胞数、划痕愈合率及Snail、N-cadherin、SPHK1、S1P、S1PR3水平显著高于HP-H组(P<0.05),E-cadherin水平显著降低(P<0.05)。结论:HP可能通过抑制SPHK1/S1P/S1PR3信号通路抑制宫颈癌细胞的增殖、侵袭和迁移。

壳寡糖与氯化稀土(Pr3＋、Dy3＋)配合物的合成、配位机理和抗羟自由基活性

用壳寡糖与氯化稀土(Pr3+、Dy3+)反应,制备了壳寡糖-镨和壳寡糖-镝配合物。用红外光谱、紫外光谱、荧光和X射线光电子能谱(XPS)等分析测试手段对配合物进行了配位机理探讨。以EDTANa2.Fe(Ⅱ)-H2O2体系产生羟自由基(.OH)来研究壳寡糖、壳寡糖-镨和壳寡糖-镝配合物对羟自由基的清除作用。结果表明,壳寡糖与Pr3+或Dy3+形成了配合物,壳寡糖-Ln配合物中壳寡糖氨基上的N原子和仲羟基的O原子参与了配位,同时Cl-也参与了配位。壳寡糖、壳寡糖-镨和壳寡糖-镝配合物对.OH均具有明显的清除作用,配合物与壳寡糖相比对.OH具有更高的抑制活性。

抗PR3抗体、抗MPO抗体检测的临床探究

[目的]探究抗蛋白酶3(PR3)抗体、抗髓过氧化物酶(MPO)抗体在系统性血管炎患者中检测的临床意义。[方法]应用酶联免疫吸附实验(ELISA)检测抗PR3抗体和抗MPO抗体,应用IIF法检测ANCA总抗体。[结果]105例系统性血管炎患者中57例韦格纳肉芽肿患者,主要表现为抗PR3抗体和颗粒型抗中性粒细胞胞质抗体(cANCA),阳性例数均为41例,阳性率均为71.93%,13例显微镜下多血管炎(MPA)和9例坏死性新月体肾炎(ATH)主要表现为MPO和pANCA,阳性率分别为53.8%和61.5%,22.22%和33.33%;135例系统性红斑狼疮(SLE)患者,主要表现为抗MPO抗体和核周型抗中性粒细胞胞质抗体(pANCA),阳性例数分别为29例和32例,阳性率分别是21.5%和23.7%。[结论]抗PR3、抗MPO抗体作为系统性血管炎的一种敏感标记抗体,有利于该疾病的早期诊断及治疗。

miR-142-3p通过调控S1PR3影响膀胱癌干细胞的干性

背景:研究发现miR-142-3p低表达于多种肿瘤组织和细胞系中,并且有研究发现TUG1/miR-142/ZEB2轴可抑制膀胱癌增殖并诱导其凋亡。目的:观察miR-142-3p在膀胱癌干细胞中的表达水平,探讨其对S1PR3的靶向调控作用及对膀胱癌干细胞干性的影响。方法:从新鲜人膀胱癌组织中分离培养原代膀胱癌细胞,采用流式细胞仪筛选出CD44^+细胞与CD44^-细胞,Western-blot检测2种细胞中Nanog、Oct4蛋白的表达,qRT-PCR检测2种细胞中miR-142-3p基因的表达。将miR-142-3p mimic(实验组)、miR对照质粒(对照组)分别转染至CD44^+细胞,48 h后,采用MTS实验检测细胞增殖能力,软琼脂克隆实验检测细胞克隆形成能力,Transwell小室实验检测各组细胞转移能力,Western-blot检测细胞中pPi3k和pAkt蛋白表达,qRT-PCR检测细胞中S1PR3基因表达。将稳定转染的2种细胞分别注射至裸鼠(购自北京维通利华实验动物技术有限公司)右侧腋下,4周后检测肿瘤体积。将S1PR3-突变型+miR-142-3p mimic、S1PR3-突变型+miR对照质粒、S1PR3-野生型+miR-142-3p mimic、S1PR3-野生型+miR对照质粒分别转染至CD44^+细胞,48h后检测各组荧光素酶活性。结果与结论:①CD44^+细胞中miR-142-3p基因表达显著低于CD44-细胞(P <0.05);CD44^+细胞中Nanog、Oct4蛋白表达显著高于CD44^-细胞,证实CD44^+细胞为膀胱癌干细胞;②实验组膀胱癌干细胞的增殖能力、克隆形成能力及转移能力明显低于对照组(P <0.05);③实验组膀胱癌干细胞中pPi3k和pAkt蛋白表达弱于对照组,S1PR3基因表达低于对照组(P <0.05);④实验组裸鼠体内肿瘤体积小于对照组(P <0.05);⑤与相比,S1PR3-突变型+miR-142-3p mimic组荧光素酶活性显著低于S1PR3-突变型+miR对照质粒组(P <0.05),S1PR3-野生型+miR对照质粒组和S1PR3-野生型+miR-142-3p mimic组荧光素酶活性无差异(P> 0.05);⑥结果表明,miR-142-3p在膀胱癌干细胞中低表达,其可能通过负调控S1PR3下调PI3K/AKT信号通路来抑制膀胱癌干细胞的干性。

蓝光激发KY(MoO4)2:Pr3+荧光粉微晶玻璃的制备及发光性能研究

通过高温固相法,在550℃下煅烧4h,制备了KY(MoO4)2:Pr3+新型红色荧光粉,通过X射线衍射(XRD)和荧光光谱(PL),研究了其结构和发光性质。结果表明:煅烧温度为550℃、煅烧时间为4 h时,样品的发光强度最好;随着Pr3+浓度的变大,样品的发光强度不断增加,当Pr3+的摩尔掺杂量为4%时,样品的荧光强度达到最大,继续增加Pr3+的浓度,由于浓度猝灭,样品发光强度降低。KY(MoO4)2:Pr3+在456 nm处可被蓝光有效地激发,样品的发射峰波长主要位于609、627、657 nm处,其中在657 nm处发射出较强的红光。在不同Pr3+掺杂浓度下,KY(MoO4)2:Pr3+的色坐标均显示出相近的值,并且均位于红色区域。KY(MoO4)2:Pr3+有望用于蓝光激发白光发光二极管(白光LED)的红色荧光粉。

烟草PR3b转录后剪切元件NRSE1与GUS融合表达后的可变剪切

【目的】烟草(Nicotiana tabacum L.)碱性几丁质酶基因PR3b在低烟碱突变体(nic1和nic2)中存在转录后mRNA可变剪切现象,但其可变剪切的发生机制仍不清楚。将PR3b的可变剪切元件NRSE1(nicotinesynthesis related splicing element 1)与GUS融合表达,分析NRSE1元件的独立可变剪切特性,以揭示其作用机制。【方法】利用PCR扩增方法获得PR3b cDNA序列中的NRSE1元件片段,并利用基因重组技术构建了烟草PR3b可变剪切元件NRSE1与GUS的融合表达载体。将融合表达载体导入农杆菌LBA4404后,通过农杆菌介导的叶盘转化法培育了表达NRSE1与GUS融合子的低烟碱突变体nic1和nic2及野生型烟草转基因植株;通过RT-PCR检测及GUS染色鉴定出阳性植株后,利用RT-PCR分析NRSE1与GUS融合表达后在低烟碱突变体和野生型烟草中的可变剪切特性;对转基因植株的幼苗进行乙烯(ET)和茉莉酸(JA)处理,通过GUS染色方法分析ET和JA处理对转基因植株中GUS活性的影响,并通过RT-PCR方法分析ET和JA处理对转基因植株中NRSE1与GUS融合子的可变剪切特性影响,以及对转基因植株中NRSE1与GUS融合子表达水平的影响。【结果】通过RT-PCR检测及GUS染色鉴定出表达NRSE1元件与GUS融合子的低烟碱突变体和野生型烟草转基因植株;RT-PCR检测及测序分析证明,NRSE1元件与GUS融合表达后仍能在低烟碱突变体发生高水平的可变剪切,剪切修饰区段的序列变化与烟草中PR3b的mRNA可变剪切修饰一致;利用ET和JA处理转基因植株进行的GUS染色表明,ET和JA处理对转基因植株的GUS活性有不同程度的影响;但利用ET和JA处理转基因植株进行的RT-PCR分析表明,ET和JA处理不改变NRSE1元件原有的诱导剪切特性,也不影响转基因植株中NRSE1元件与GUS融合子的表达水平。【结论】PR3b的可变剪切元件NRSE1与GUS在烟草中融合表达后,仍能在低烟碱突变体nic1和nic2中发生高水平的可变剪切;NRSE1在烟草中的可变剪切不依赖PR3b的其他mRNA区段,是烟草PR3b发生可变剪切的独立元件;ET和JA处理对NRSE1元件与GUS融合表达植株的GUS活性具有一定影响,可能存在翻译水平的调控作用。

Adsorption properties of a novel 3D graphene/MgO composite for heavy metal ions

A novel three-dimension(3D)graphene/MgO composite was synthesized through self-assembly and embedding MgO nanoparticle in reduced graphene in situ.Fourier transform infrared(FT-IR)spectroscopy,thermal gravimetric analysis(TGA),scanning electron microscopy(SEM),transmission electron microscope(TEM),powder X-raydiffraction(XRD)and X-rayphotoelectron spectroscopy(XPS)were employed to characterize the prepared 3D graphene/MgO composite.The adsorption performance of some metal ions on 3D graphene/MgO was investigated.The results showed that the adsorption capacity was greater than 3D graphene and the maximum adsorption capacity at 25℃was found to be 358.96 mg/g,388.4 mg/g and 169.8 mg/g for Pb^2+,Cd^2+and Cu^2+,respectively.The adsorption kinetic conformed to the pseudo-second-order kinetic model and the adsorption isotherm was well described by Langmuir model.The thermodynamic constants revealed that the sorption process was endothermic and spontaneous in nature.Based on the results of the removal of heavy metal ions from metal smelting wastewater,it can be concluded that the prepared 3D graphene/MgO composite is an effective and potential adsorbent.

Regio-and Stereo-selective Reductions of Steroidal 4-en-3,6-dione

The different regioselective and stereoselective products were obtained by the reduction of stigmast-4,22-dien-3,6-dione with NaBH_4-CH_3OH when different kinds of metal ions was added to the reaction.

Improvement of Luminescent Properties of PbWO_4 by Doping with Gd^(3+) Ions

The luminescent properties of PbWO 4∶Gd 3+ were studied. The luminescence of Gd 3+ in PbWO 4∶Gd 3+ was quenched. It is possible that the excitation states of Gd 3+ locate in the conduction band of PbWO 4 crystal. The luminescent intensity of the green and the blue band of PbWO 4 emission increases by doping with about 0 005% and 0 01% (molar fraction) Gd 3+ respectively. Mechanism of this enhancement of PbWO 4∶Gd 3+ luminescence is probably due to energy transfer from Gd 3+ to PbWO 4 host in the crystal. The PbWO 4 doped with low concentration of Gd (about 0 005%～0 01%) is a good scintillating material.

敲除S1PR3可缓解小鼠的急性肺损伤:基于抑制MAPK信号通路

目的探讨敲除S1PR3是否通过抑制丝裂原活化蛋白激酶(MAPKs)途径改善脂多糖(LPS)诱导的小鼠急性肺损伤。方法用LPS诱导小鼠急性肺损伤模型。雄性C57BL/6J和S1PR3敲除小鼠,随机分为4组:C57 NS组(C57,生理盐水处理)、C57LPS组(C57,LPS诱导)、S1PR3^(-/-)NS组(S1PR3敲除鼠,生理盐水处理)、S1PR3^(-/-)LPS组(S1PR3敲除鼠,LPS诱导),8只/组。RT-qPCR检测S1PR3,IL-β和IL-18表达,HE染色检测肺部组织损伤,流式细胞术检测细胞凋亡水平,Western blot法检测caspase-1,GSDMD,p-JNK,p-ERK p-p38蛋白表达水平。同时,Ⅱ型肺泡上皮细胞(MLE-12细胞)铺板后分为4组:PBS组、LPS组、CYM5541组(仅加入S1PR3激动剂)、CYM5541+LPS组(加入S1PR3激动剂+LPS),检测各组细胞焦亡水平及MAPK信号通路分子的表达水平。结果急性肺损伤小鼠肺组织S1PR3表达上调(P<0.001);血清IL-1β和IL-18因急性肺损伤而明显升高(P<0.05)。急性肺损伤小鼠因敲除S1PR3肺出血、炎症渗出明显改善,肺湿干比重下降,细胞凋亡比例和焦亡相关蛋白如clvcaspase-1,GSDMD表达均降低(P<0.05)。MLE-12细胞因加入S1PR3激动剂,细胞焦亡相关蛋白表达均升高(P<0.05)。此外,MAPKs家族(JNK,ERK p38)的激活因S1PR3敲除后受到抑制,因加入S1PR3激动剂而表达明显升高(P<0.05)。结论敲除S1PR3可通过抑制MAPK信号通路改善急性肺损伤。

Extraction of lanthanide ions with N,N,N′,N′-tetrabutyl-3-oxadiglycolamide from nitric acid media

Extractability and extraction mechanism of lanthanide ions were investigated by using a new extractant,N,Nn,N′,N′-tetrabutyl-3-oxa-diglycolamide(TBDGA),in toluene from nitric acid media.The effects of HNO_3 and TBDGA concentrations,and temperature,on extraction of lanthanide ions were studied.Stoichiometrics of the main extracted species were HNO_3·TBDGA and M(NO_3)_3-3TBDGA(M = Er,Dy,Tb,Gd,La,Ce,Nd,Sm and Eu).The extracted species for metal ions were established to be ionic complex.In this complex,nitrate anion was not coordinated to the central ion.The extraction pattern increased gradually across the lanthanide ions series,showing enhanced affinity of TBDGA toward heavy lanthanide ions.Thermodynamic parameters were investigated for the exothermic extraction reaction.

Optical Spectroscopy of Pr3+ Ion Singly Doped LiLuF4 Single Crystal by Bridgman Method

High quality LiLuF4 single crystals doped with various Pr3+ ions were synthesized by a vertical Bridgman method in completely sealed platinum crucibles. The excitation spectra spans from 420 nm to 500 nm. The prepared single crystals exhibit a blue band at 480 nm(3P0→3H4), a green band at 522 nm (3P1→3H5), and a red band at 605 nm (1D2→3H4)when excited at 446 nm;their corresponding average lifetimes are 38.5μs, 37.3μs, and 36.8μs, respectively, which are much longer than those in oxide single crystals. The effects of excitation wavelength and doping concentration on emission intensities and chromaticity coordinates are investigated. The optimal Pr3+ concentration is confirmed to be 0.5%.The temperature dependent emission shows that the emission intensity constantly decreases with the increase of temperature from 298 K to 443 K due to the enhancement of nonradiative quenching at high temperature. The 3P0→3H4 transition is the most vulnerable to temperature, followed by the 3P1→3H5 transition and 1D2→3H4 transition.

Engineering Leaf-Like UiO-66-SO3H Membranes for Selective Transport of Cations

Metal–organic frameworks(MOFs)with angstrom-sized pores are promising functional nanomaterials for the fabrication of cation permselective membranes(MOF-CPMs).However,only a few research reports show successful preparation of the MOF-CPMs with good cation separation performance due to several inherent problems in MOFs,such as arduous selfassembly,poor water resistance,and tedious fabrication strategies.Besides,low cation permeation flux due to the absence of the cation permeation assisting functionalities in MOFs is another big issue,which limits their widespread use in membrane technology.Therefore,it is necessary to fabricate functional MOF-CPMs using simplistic strategies to improve cation permeation.In this context,we report a facile in situ smart growth strategy to successfully produce ultrathin(<600 nm)and leaflike UiO-66-SO3H membranes at the surface of anodic alumina oxide.The physicochemical characterizations confirm that sulfonated angstrom-sized ion transport channels exist in the as-prepared UiO-66-SO3H membranes,which accelerate the cation permeation(~3×faster than non-functionalized UiO-66 membrane)and achieve a high ion selectivity(Na^+/Mg^2+>140).The outstanding cation separation performance validates the importance of introducing sulfonic acid groups in MOF-CPMs.

家族性地中海热伴PR3-ANCA阳性反复发作脑血管病1例

报道1例家族性地中海热伴PR3-ANCA阳性反复发作脑血管病患者的临床资料,并进行文献复习。

Rare earth doped CaCu_3Ti_4O_(12) electronic ceramics for high frequency applications

Ca1-xRxCu3Ti4O12(R=La,Y,Gd;x=0,0.1,0.2,0.3) electronic ceramics were fabricated by conventional solid-state reaction method.The microstructure and dielectric properties as well as impedance behavior were carefully investigated.XRD results showed that the secondary phases with the general formula R2Ti2O7 existed at grain boundaries of rare earth doped ceramics,which inhibited abnormal grain growth.The dielectric constant decreased from 4×105 in pure CaCu3Ti4O12(CCTO) ceramics to 2×103 with rare earth doping....

全自动流式点阵免疫发光法测定抗MPO和抗PR3抗体的性能评估

目的评估全自动流式点阵免疫发光法(MBIA法)测定抗髓过氧化物酶(MPO)和抗蛋白酶3(PR3)抗体的检测性能。方法按照美国临床实验室标准化委员会(CLIS)EP15-A3文件进行精密度验证,按照EP28-A3文件验证参考区间。比较MBIA法和ELISA法检测抗PR3和抗MPO结果,结合间接免疫荧光法(IFT)评价不同方法对抗中性粒细胞胞浆抗体(ANCA)相关性血管炎(AAV)的诊断效能。结果 MBIA法检测抗PR3和MPO抗体批内、批间不精密度分别为3.65%、4.10%和2.28%、3.32%,小于厂家声明允许不精密度批内4.70%、批间8.60%,符合要求。MBIA和ELISA两种方法检测抗PR3和抗MPO的阳性、阴性、总符合率分别是40.00%、90.89%、90.72%和80.49%、97.65%、96.10%。MBIA法和ELISA法的敏感性、特异性以及阳性预测值分别是53.85%、91.69%、67.31%和55.38%、79.71%、46.45%。与IFT结果结合后,阳性预测值可分别提高至83.56%、73.48%。结论 MBIA法检测性能符合要求,其诊断AAV的效能要优于ELISA法,与IFT法结果结合可提高对AAV诊断的阳性预测值。