Purpose: Description of a patient with a solitary cyst of the pupillary margin iris pigment epithelium (IPE). Methods: A 63-year-old man referred a suspected iris-ciliary body melanoma in his left eye. Based on both c...Purpose: Description of a patient with a solitary cyst of the pupillary margin iris pigment epithelium (IPE). Methods: A 63-year-old man referred a suspected iris-ciliary body melanoma in his left eye. Based on both clinical examination and ultrasound biomicroscopy, melanoma was considered unlikely. Surgery was under-taken to correct recurrent deterioration of vision due to movement of the lesion across the visual axis. Results: The lesion was excised completely. Ultrasound biomicroscopy and histopathological examination ruled out melanoma and allowed a final diagnosis of primary pupillary margin cyst of the IPE, characterized of pig-mented epithelium, with no connective tissue or vessels. No recurrences or fresh lesions appeared during a one-year follow-up. Conclusions: Primary epithelial iris cysts are usually benign. Treatment is required only in symptomatic patients and those with an uncertain diagnosis. Ultrasound biomicroscopy is indispensable to confirm the clinical diagnosis, follow the clinical course and intervene if surgery is required.展开更多
Purpose: To study the feasibility of adeno-associated virus mediated gene transfection tocultured human iris pigment epithelium (IPE) cells in vitro.Methods: Recombinant replication deficient adeno-associated viruses ...Purpose: To study the feasibility of adeno-associated virus mediated gene transfection tocultured human iris pigment epithelium (IPE) cells in vitro.Methods: Recombinant replication deficient adeno-associated viruses (AAV) expressingLacZ gene were produced without helper virus. The LacZ gene was transduced into culturedhuman IPE cells.Results: Cultured human IPE cells stained positively anticytokeratin, The titer ofrAAV-LacZ was 2.1 × 108 virus particles/ml, 42% cultured human IPE cells expressedβ-galactosidase 7 days after transfection and 67% after 14 days.Conclusions: Recombined AAV produced without helper virus can transfer a foreign geneinto human IPE cells with high efficiency in vitro.展开更多
Purpose; To study the human iris pigment epithelial cells cultivation in vitro. Methods: The iris pigment epithelial cells were isolated by scraping directly or by enzyme digestion and cultured in vitro. The cells wer...Purpose; To study the human iris pigment epithelial cells cultivation in vitro. Methods: The iris pigment epithelial cells were isolated by scraping directly or by enzyme digestion and cultured in vitro. The cells were observed under the transmission electron microscope and analized by means of immunohistochemical assay.Results: The cells obtained by scraping were easier to grow and to be passaged than those obtained by enzyme digestion. The primary cells appeared multigonal and arranged in mono-layer. Abundant pigment granules were in the cytoplasm and diminished in succeeding generations . The maculae occludentes and desmosomes and microvilli which were characteristics of epithelial cells could be found under the electronmicroscope. The immunohistochemical assay showed positive reaction of keratin antigen in cultured cells.Conclusion: The human iris pigment epithelial cells could be cultured in vitro and the cells in primary generation some characteristics be preserved. Eye Science 1997; 13: 137 -展开更多
文摘Purpose: Description of a patient with a solitary cyst of the pupillary margin iris pigment epithelium (IPE). Methods: A 63-year-old man referred a suspected iris-ciliary body melanoma in his left eye. Based on both clinical examination and ultrasound biomicroscopy, melanoma was considered unlikely. Surgery was under-taken to correct recurrent deterioration of vision due to movement of the lesion across the visual axis. Results: The lesion was excised completely. Ultrasound biomicroscopy and histopathological examination ruled out melanoma and allowed a final diagnosis of primary pupillary margin cyst of the IPE, characterized of pig-mented epithelium, with no connective tissue or vessels. No recurrences or fresh lesions appeared during a one-year follow-up. Conclusions: Primary epithelial iris cysts are usually benign. Treatment is required only in symptomatic patients and those with an uncertain diagnosis. Ultrasound biomicroscopy is indispensable to confirm the clinical diagnosis, follow the clinical course and intervene if surgery is required.
文摘Purpose: To study the feasibility of adeno-associated virus mediated gene transfection tocultured human iris pigment epithelium (IPE) cells in vitro.Methods: Recombinant replication deficient adeno-associated viruses (AAV) expressingLacZ gene were produced without helper virus. The LacZ gene was transduced into culturedhuman IPE cells.Results: Cultured human IPE cells stained positively anticytokeratin, The titer ofrAAV-LacZ was 2.1 × 108 virus particles/ml, 42% cultured human IPE cells expressedβ-galactosidase 7 days after transfection and 67% after 14 days.Conclusions: Recombined AAV produced without helper virus can transfer a foreign geneinto human IPE cells with high efficiency in vitro.
文摘Purpose; To study the human iris pigment epithelial cells cultivation in vitro. Methods: The iris pigment epithelial cells were isolated by scraping directly or by enzyme digestion and cultured in vitro. The cells were observed under the transmission electron microscope and analized by means of immunohistochemical assay.Results: The cells obtained by scraping were easier to grow and to be passaged than those obtained by enzyme digestion. The primary cells appeared multigonal and arranged in mono-layer. Abundant pigment granules were in the cytoplasm and diminished in succeeding generations . The maculae occludentes and desmosomes and microvilli which were characteristics of epithelial cells could be found under the electronmicroscope. The immunohistochemical assay showed positive reaction of keratin antigen in cultured cells.Conclusion: The human iris pigment epithelial cells could be cultured in vitro and the cells in primary generation some characteristics be preserved. Eye Science 1997; 13: 137 -
